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DETEKSI GEN COA PADA STAPHYLOCOCCUS AUREUS YANG DIISOLASI DARI SUSU SAPI MURNI Wahyuni, Rizka Ayu; Darmawati, Sri; Prastiyanto, Muhammad Evy
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tujuan penelitian ini untuk mendeteksi gen Coa pada S. aureus yang diisolasi dari susu sapi murni di Kelurahan Gedawang. Jenis penelitian ini adalah penelitian deskriptif. Bakteri S. aureus di isolasi dari 15 sampel susu sapi murni. Deteksi gen Coa S. aureus menggunakan primer spesifik  Forward (5´-ATAGAGATGCTGGTACAGG-´3) dan primer Coa Reverse (5´GCTTCCGATTGTTCGATGC-3´).Jumlah isolat S. aureus yang didapatkan dari Isolasi Bakteri sebanyak 3 sampel positif yang memiliki gen Coa dengan hasil produk 756 bp. Hasil pada penelitian ini menunjukkan hasil positif gen Coa sebesar 100%.   Keywords: Staphylococcus aureus, Susu, gen Coa
MOLECULAR CHARACTERIZATION AND HEMAGGLUTINATION ACTIVITIES OF FLAGELLIN PROTEIN OF SALMONELLA TYPHI Darmawati, Sri; Santosa, Budi; Prastiyanto, Muhammad Evy; Saptaningtyas, Ragil
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2016: Proceeding of International Seminar on Education Technology (ISET) 2016
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Abstract. The purposes of this research are for molecular characterization and hemagglutination activity test of flagellinprotein of Salmonella typhi. The research samples consist of 7 strains of S. typhi isolates from Central Java (5 strains from Semarang city, 1 strain from Salatiga and 1 strain from Magelang) and 2 strains of S. typhi from Yogyakarta (Doctor Sardjito Hospital and Bethesda Hospital). The undertaking procedures are: 1) PCR and sequencing of fliC genes using primer LPW 1856 and LPW 1857.2) Isolation and separation of flagellin protein using SDS-PAGE. 3) Hemagglutination Activity Test upon human erythrocytes of blood group A, B, AB and O.The results show that 8 strains of S. typhi have a fliC gene size of 1452 to 1488 bp including serovar H1-d, and 1 strain with the size of 1267 bp including serovar H1-J. Flagella protein resulted from SDS-PAGE protein consists of 1-2 major proteins and 1-3 minor proteins with a molecular weight of 16-116 kDa. The results of hemagglutination activity test of flagellin protein show that there are 3 strains of S. typhi (MG-1, SA02.2 and BET) which are able to agglutinate human erythrocytes of bloodgroup A, B, AB and O (2-64HA), 6 other strains show various hemagglutination activities varied
AKTIVITAS ANTIBAKTERI MADU TERHADAP BAKTERI MULTI DRUG RESISTANT SALMONELLA TYPHI DAN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS Panjaitan, Rahma Asriani; Darmawati, Sri; Prastiyanto, Muhammad Evy
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

This study aimed to analyze the antibacterial activity of Palm oil Honey against Multi  Drug  Resistant  Salmonella  typhi  and  Methicillin-Resistant Staphylococcus aureus by concentration 50%, 60%, 70%, 80%, 90% and 100%. This  research  was  an  experimental  test  with  Posttest  Only  Control  Group Design in an in vitro manner using well diffusion method techniques. The well diffusion method used MHA which made by the well of 5 mm diameter and inserted 200 µ L sample then incubated 35 ± 20C for 24 hours. The results of the study showed the antibacterial activity of Palm oil Honey with zone inhibition against MDR S.typhi 11.4 mm (concentration 90%), 13.4 mm (concentration 100%) and against MRSA 11.7 mm (concentration 100%). The results of zone inhibition of Palm oil honey with concentration 100% against MDR S.typhi was larger than the  zone inhibition  against MRSA. The palm oil honey inhibition zone  against  S.typhi  MDR  bacteria  compared  with  sulfamethoxazole  (SXT) antibiotics with 25 mm inhibition zones was included in the intermediate category and the palm oil honey inhibition zone against MRSA bacteria compared with Tetracycline (TE) antibiotics with 23 mm inhibition zone was included in resistant category. Keywords: Antibacterial Activity, MDR S. typhi, MRSA, Honey, Inhibition Zone
KEANEKARAGAMAN DAN POLA RESISTENSI BAKTERI PADA PASIEN YANG TERDIAGNOSA SEPSIS Batara, Murni; Darmawati, Sri; Prastiyanto, Muhammad Evy
Jurnal Labora Medika Vol 2, No 2 (2018): Jurnal Labora Medika
Publisher : Jurnal Labora Medika

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Abstract

Sepsis atau Systemic Inflammatory Response Syndrome (SIRS) adalah kondisi klinis akut dan serius yang muncul akibat adanya infeksi bakteri di dalam darah. Penelitian ini bertujuan untuk mengetahui keanekaragaman bakteri dan pola resistensi terhadap antibiotik pada sampel darah pasien yang terdiagnosa sepsis  di Laboratorium Klinik Swasta di Semarang pada bulan Mei-Juni tahun 2018. Metode penelitian ini yaitu deskriptif. Hasil penelitian didapatkan sebanyak 42 sampel darah yang ditanam pada media Bact Alert, 12 sampel dinyatakan positif tumbuh. Dari ke 12 sampel tersebut didapatkan 6 jenis bakteri penyebab sepsis yaitu Staphylococcus haemolitycus (25%), Staphylococcus aureus (16,66), Staphylococcus hominis (16,66%), Staphylococcus epidermidis (16,66%), Staphylococcus saprophyticus (8,33%),  Pantoea sp (8,33%), dan Bordetella hinzii (8,33%). Bakteri gram positif resisten terhadap antibiotik Benzylpenicillin, Oxacillin, Penicyline, dan antibiotik betalaktam lainnya. Sedangkan bakteri Pantoea sp resisten terhadap antibiotik Pipericillin/Tazobactam dan Bordetella hinzii resisten terhadap antibiotik Pipericillin/Tazobactam dan Nitrofurantoin
ANTIBACTERIAL ACTIVITY OF PARIJOTO FRUIT EXTRACT (MEDINILLA SPECIOSA) ON BACTERIA EXTENDED SPECTRUM BETALACTAMASE (ESBL) ESCHERICHIA COLI AND METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) Wahyuni, Rizka Ayu; Putri, Inesya Yuliana; Jayadi, Eden Lambang; Prastiyanto, Muhammad Evy
Jurnal Media Analis Kesehatan Vol 10, No 2 (2019): JURNAL MEDIA ANALIS KESEHATAN
Publisher : poltekkes kemenkes makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1262.478 KB) | DOI: 10.32382/mak.v10i2.1250

Abstract

Infection is one of the causes of death and pain that occurs mainly in Indonesia. Infections can be caused by Gram-negative and gram-positive bacteria such as the Extended Spectrum Beta Laktamase, Escherichia coli (E. coli) and Methicilin Resistant Staphylococcus aureus (MRSA), which are one of the Strain of S. aureus. The study aims to determine the antibacterial activity of Aquadest extracts, Methanol, Ethyl acetat and N-hexane of Parijoto (M. speciosa), MIC and MBC values against ESBL E. coli and MRSA bacteria. The samples in this study were Parijoto fruit extracts concentration 100 mg/mL. In this research antibacterial activity using the method of diffuse with the MHA media, while to know the value of MIC using methods dilution with MHB and MBC media using BAP media. The results were characterized by the formation of the largest barrier zone on the 11.6 mm methanol extract, the smallest MIC yield on N-hexane and aquadest extracts with a value of 50 mg/mL, whereas on MBC tests each extract resulted in a value of 50 mg/mL and While the hate zone against the MRSA bacteria is obtained the largest diameter of the hate zone in the N-hexane solvent, which is 23.17 mm in average, the largest MIC value in the aquadest solvent at a concentration of 50 mg/ml, and the largest MBC value in methanol solvent Concentrations of 50 mg/ml. So that it can be concluded Parijoto fruit extract has the potential as an alternative substitute for chemical antibiotics against MRSA bacteria whereas for bacteria ESBL E. coli has no potential as an alternative to antibiotic substitute.Keywords : Antibacterial, Escherichia coli, Methicilin Resistant Staphylococcus aureus, Medinilla speciosa
ANTIBACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF AVERRHOA BILIMBI AGAINST MULTIDRUG RESISTANT BACTERIA Prastiyanto, Muhammad Evy; Wardoyo, Fandhi Adi; Wilson, Wildiani; Darmawati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020 Articles in press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23600

Abstract

The multi-drug resistance (MDR) bacteria is a global health problem that causes high mortality every year. Therefore, novel antibacterial agents are needed from natural biological sources. This research aimed to investigate the antibacterial activities of various crude extracts of Averrhoa bilimbi against MDR bacteria. The antibacterial activity was calculated based on the use agar well diffusion assay and the minimum bactericidal concentration (MBC) using Mueller?Hinton broth in a microdilution method. Bacteria from wells were subcultured using inoculating loop onto a 5% sheep BAP. The best antibacterial activity, calculated as the most widely inhibitory zone and the smallest MBC values. The ethanolic extract showed antibacterial activity against the all MDR bacterial test in the agar well diffusion assay (10-14.5 mm inhibition diameter). The MBC of water extract against ES?L + CR Pseudomonas aeruginosa showed the best antibacterial activity (12.5 mg/mL). The fruit of bilimbi was shown to be potentially developed as antibacterial agents, especially for MDR strains. Further in vivo research and discovery of action mode are needed to shed light on their antibacterial effects. This study can provide new information about the benefits of bilimbi as a source of natural antibacterial againts MDR-bacteria