Agistiana, Seruni
Unknown Affiliation

Published : 2 Documents

Found 2 Documents

EQ-1 QUARANTINE ACTION FOR HORSES ORIGINATING FROM COUNTRIES WITH DIFFERENT HEALTH STATUS TO EDFZ JAKARTA FOR THE 18TH ASIAN GAMES 2018 Mujiatun, .; Silitonga, Risma Juniarti Paulina; Sinanto, Agus; Sriyanto, .; Munstermann, Susanne; Wahyuni, Tri; Deniswaty, .; Zaenuddin, Nuryani; Dewi, Arum Kusnila; Komara, Adi; Kalanjati, Woro Wulandari; Qurotaayunina, Yasmin; Agistiana, Seruni; Haeriah, .; Prastanti, Hestu; Anjarsari, Dwi Indah; Anastasya, Lidwina; Ashari, .
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.96 KB)


Indonesia hosted the 18th Asian Games on 18 August to 2 September 2018. In order to organize the equestrian competitions of these Asian Games, an Equine Disease Free Zone (EDFZ) was set up. EDFZ is a term used by the World Organisation for Animal Health (OIE) for a specific zone in which equestrian competitions can be organized. This system is based on the OIE?s concept of compartmentalisation and zoning. A specified area is shown to be free from a defined list of equine diseases that are otherwise present in the country or region. Horses within an EDFZ are protected from diseases that may occur in other parts of the country (OIE 2018).Once the EDFZ is established, implementation of biosecurity management, health certification standards and procedures have to be observed, including the setting up of a Registered Animal Quarantine Installation (RAQI) to prevent the spread of diseases to this EDFZ.Horses that came from countries that have a disease situation equal to the EDFZ with specific health requirement such as vaccination and testing, were taken directly to the EDFZ core zone, which was the venue for the equestrian events at the Jakarta Equestrian Park, Pulomas [DGLAHS, 2018].  Few horses came from countries with a health status not equal to the EDFZ. These horses had to be quarantined in their country of residence for 21-30 days and again in Indonesia for 14-21 days. Based to the regulations of the Indonesian Agriculture Quarantine Agency (IAQA) a RAQI was set up to observe the health status of these horses.General observation of horse health status in the RAQI consisted of inspection for clinical signs of infectious diseases and also a repeat of laboratory testing for specified diseases which, as per requirement of the ?Veterinary Certificate for the Temporary Importation of horses into Indonesia to compete in the equestrian events of the 18th Asian Games? (here: Asian Games Health Certificate), had already been tested in the country of residence.The purpose of this paper is to describe the quarantine action for horses in the RAQI at the 18th Asian Games 2018.
Application of Multiplex PCR Assay for Campylobacter fetus Sub sp. Detection and Differentiation in Bovine Preputial Wash Agistiana, Seruni; Setiyaningsih, Surachmi; Indrawati, Agustin
Buletin Peternakan Vol 42, No 1 (2018): BULETIN PETERNAKAN VOL. 42 (1) FEBRUARY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (25.883 KB) | DOI: 10.21059/buletinpeternak.v42i1.26114


The aim of this research was to evaluate the detection and differentiation of C. fetus subspecies through multiplex PCR (mPCR) method from the bovine preputial wash. The evaluation was done by comparing 2 extraction methods, which was by commercial kit extraction and boiling method, and also by measuring the effect of Transport Enrichment Media (TEM) addition as bacterial enrichment. Spiked bovine preputial washes with 2 extraction methods (commercial kit and boiling), with and without TEM addition were used as a sample. The results showed that the highest sensitivity/specificity was by boiling extraction without TEM addition, which reached 83.3%/100% for all or grouped sample. The results also supported by Poisson regression analysis which showed the comparison between sample with and without TEM addition which had 1.3 Incidence Rate Ratio (IRR). The obtained IRR score indicated that sample without TEM addition had 30% better result compared to the sample with TEM addition. Furthermore, a comparison between commercial kit and boiling extraction showed no significant difference (1.029 IRR). The research concluded that boiling extraction without TEM addition is the most accurate, fast and economical extraction method regarding its high sensitivity and specificity value, with less than 24 hours testing time and without any commercial kit requirements. However, a validation and verification with field sample were still needed before applied in the laboratory.