Hermana, Nabila Swarna Puspa
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MP-7 ISOLATION OF ANTIBIOTIC RESISTANCE GENE FROM STAPHYLOCOCCUS AUREUS FROM CHICKEN POULTRY FARM IN BOGOR WEST JAVA INDONESIA Hermana, Nabila Swarna Puspa; Indrawati, Agustin; Pasaribu, Fachriyan Hasmi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Staphylococcus aureus is an opportunistic pathogen in human and another animal species. It also associated with staphylococcosis, osteomyelitis, arthritis, and bumble foot disease in poultry. Antibiotics including penicillin, erythromycin and tetracycline are widely used for treating infection caused by S. aureus.Chicken poultry play important role in food supply as one of protein source in Indonesia. West Java province has largest population of chicken poultry, where Bogor has the largest population of chicken poultry with 19.75.850 chicken in 2018. In order to maintain the production, farmers used antibiotics for prophylaxis, growth promotor, and therapy. However, extensive use of antibiotic can be found in low income and developed country, caused the increasing number of antibiotic resistant bacteria.The aim of this study is to determine the antibiotic resistance pattern of S. aureus isolated from cloacal swab of chicken poultry in Bogor West Java Indonesia.
EKSTRAKSI PROTEIN DARI ESCHERICHIA COLI BL21 REKOMBINAN GEN MYCOBACTERIUM TUBERCULOSIS DENGAN VARIASI WAKTU INKUBASI INDUKSI ISOPROPHYL-β-D-THIOGALACTOSIDASE (IPTG) DAN METODE LISIS SEL Hermana, Nabila Swarna Puspa; Kusdiyantini, Endang; Suprihadi, Agung; Nuraini, Neny
Jurnal Akademika Biologi Vol. 4 No. 2 April 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacillus Calmete-Guerin vaccine is no more effective in preventing tuberculosis. The vaccine developed now is vaccine recombinant using Mycobacterium tuberculosis gen cloned in Escherchia coli to produce protein which is used as antigen compound in vaccine. The protein produced an intercellular protein which is needed cell lysis process so that the protein could be extracted. The aim of this research was to investigate the incubation time, and the optimum cell lysis method in protein recombinant extraction. Recombinant E. coli BL21 was incubated for 4, 8, 12, and 16 hours, and followed by, freeze thaw, sonication, and beads vortex lysis method. SDS PAGE quantification showed that the sample with 12 hour incubation with freeze thawing lysis method had the highest protein recombinant concentration by 4.327.870,4 pixel. Keywords: E. coli BL21 recombinant, protein extraction, enzymatic, freeze thaw, sonication, beads vortex