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Pengaruh Jarak Penyekat dalam Fotobioreaktor Tubular terhadap Pertumbuhan Kultur Mikroalga Ankistrodesmus convulutus Chrismadha, Tjandra; Rustiani, Tenni; Rosidah, Rosidah; Mardiati, Yayah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.514 KB) | DOI: 10.24002/biota.v12i1.2530

Abstract

A series of partition was constructed inside a vertical tubular photobioreactor to control the culture distribution in obtaining intermittent exposure to light area in the surface of the tubular column. A microalga, Ankistrodesmus convulutus, was grown in a batch mode in the reactor with PHM medium, light sources of fluorescent lamp 3 x 40 watt (I = 5.500 lux on the surface of the tubular) and room temperature of 27 – 310C. The experiment was carried out to study the influence of partition density which was constructed at varied distance, which were 1, 2 and 3 inch on the growth and biochemical composition of the alga. A vertical tubular photobioreactor without partition was applied as the control. The partition density did not significantly affect the optical density achievement of the culture, but it significantly affected the biomass and chlorophyll concentration, as well as the protein/carbohydrate ratio of the culture. It is believed that the intermittent exposure of algal cell to the light area leads to sincronization of the light and dark reaction of the photosynthesis, as well as to vapor the catalitic proccesses to develop the functional cell structure.
PENYISIHAN NUTRIEN DENGAN KULTUR Eichornia Crassipes DALAM AIR LIMBAH KOLAM IKAN RESIRKULASI SEMI TERTUTUP Satya, Awalina; Chrismadha, Tjandra; Sulawesty, Fachmijany; Yoga, Gunawan P.; Mardiyati, Yayah
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 21, No 2 (2014)
Publisher : Research Center for Limnology

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Abstract

Air Limbah kolam ikan resirkulasi memiliki karakteristik yang mirip dengan perairan eutrofik sehingga tidak aman untuk dibuang ke perairan umum secara langsung. Pada penelitian ini dilakukan evaluasi kemampuan tiga variasi kepadatan eceng gondok (Eichornia crassipes) untuk memperbaiki kualitas air limbah kolam ikan resirkulasi tersebut. Tujuan penelitian adalah untuk 1) mengungkapkan dinamika kualitas air dalam ?batch culture? 2) kinetika laju reduksi nutrien nitrogenik-fosforik dan konstituen pencemar lainnya, dan 3) efektifitas penyisihan nutrien. Eksperimen terdiri atas empat bak plastik, B1, B2, B3 dan B4. Bak B1 merupakan kontrol, hanya berisi air limbah tanpa eceng gondok. Bak B2, B3 dan B4 berisi eceng gondok dengan kepadatan awalnya berturut-turut adalah 1.618,40 gram/m2; 2.436,51 gram/m2 ; dan 3.243,93 gram/m2. Percobaan dilakukan selama empat hari. Pengukuran pH,Oksigen terlarut (Dissolved Oxygen,DO), konduktifitas, suhu, Total Dissolved Solid (TDS), dan % DO saturation dilakukan tiga kali sehari pada jam 09.00-09.30 ; 12.00-12.30 dan 16.00-16.30, sedangkan Senyawa nutrien nitrogenik (N-NH3+; N-NO2-; N-NO3-, Total Nitrogen (TN)), fosfor (TP), Total Suspended Solid (TSS) dan Total Organic Matter (TOM) dianalisis dua kali sehari pada pagi dan sore hari. Reduksi parameter konduktifitas,TDS, nutrien, nitrogenik, dan fosforik dalam bak-bak yang ditumbuhi eceng gondok mengikuti model kinetika order pertama. Kepadatan eceng gondok ideal adalah 2.436,51 gram/m2 (bak B3) karena menghasilkan pertambahan densitas yang paling tinggi (147,13 gram/m2). Bak berisi eceng gondok paling efektif menyisihkan turbiditas (94,28%-100%), N-nitrit (98,21%-98,93%), TP (92,86%-93,62%), N-nitrat (58,33%-83,33%), TN (59,46%-66,06%), N-ammonia (18.82%-46,88%) dan konduktifitas (16,34%-23,54%). Seluruh perlakuan dan kontrol terbukti tidak efektif untuk menyisihkan material organik.
LAJU PERTUMBUHAN IKAN MAS (Cyprinus carpio L) DENGAN PEMBERIAN PAKAN LEMNA (Lemna perpusilla TORR.) SEGAR PADA KOLAM SISTEM ALIRAN TERTUTUP Sulawesty, Fachmijany; Chrismadha, Tjandra; Mulyana, Endang
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 21, No 2 (2014)
Publisher : Research Center for Limnology

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Abstract

Penelitian telah dilakukan untuk meningkatkan kinerja budidaya perikanan melalui pemanfaatan pakan alternative yang murah dan ramah lingkungan. Pakan alternatif yang dipilih adalah jenis tumbuhan air, lemna (Lemna perpusilla Torr) yang dikultur secaraintegratif pada suatu sistem produksi perikanan sehingga mempunyai fungsi sebagai sumberpakan alami sekaligus menyerap bahan pencemar kegiatan budidaya. Pengamatan inibertujuan untuk melihat pengaruh pemberian lemna segar terhadap laju pertumbuhan ikan mas(Cyprinus carpio L) pada kolam sistem aliran tertutup. Biomassa lemna segar digunakan untukmengantikan pellet pada porsi setara 50% dari berat kering pakan yang diberikan pada ikanmas sebanyak 3% dari berat badannya per hari. Pengamatan dilakukan selama 24 minggu.Ukuran berat awal ikan mas rata-rata 4,75 - 6,5 g dngan padat penebaran100 ekor per wadah.Parameter pengamatan adalah berat rata-rata ikan,, laju pertumbuhan spesifik, rasio konversipakan, faktor kondisi, dan tingkat kelangsungan hidup ikan. Pemberian campuran lemna segarpada pakan ikan mas memberikan nilai laju pertumbuhan spesifik lebih tinggi, yaitu sebesar2,00 %/hari, dibandingkan dengan yang hanya diberi pakan pellet (1,75 %/hari). Berat ratarata,nilai konversi pakan, dan tingkat kelangsungan hidup ikan yang diberi pakan campuranlemna segar bertutut-turut: 162,7 g, 2,00, dan 64 %, lebih tinggi dibandingkan dengan ikanyang diberi ransum pellet saja (berturut-turut 108,9 g, 3,34, dan 61 %). Pertumbuhan ikanbersifat allometrik negatif (nilai b < 3) dengan nilai b pada ikan yang diberi pakan campuranpellet dan lemna segar lebih tinggi (2,737) dibandingkan dengan ikan yang diberi pakan pelletsaja (2,537). Hasil ini mengindikasikan adanya pengaruh positif pemberian lemna segar yaitudapat meningkatkan pertumbuhan ikan mas. Pemanfaatan biomassa lemna segar untukcampuran pakan ikan mas dapat meningkatkan efisiensi produksi pada usaha budidaya ikanmas.
PENGARUH KONSENTRASI NITROGEN DAN FOSFOR TERHADAP PERTUMBUHAN, KANDUNGAN PROTEIN, KARBOHIDRAT DAN FIKOSIANIN PADA KULTUR SPIRULINAFUSIFORMIS Chrismadha, Tjandra; Panggabean, Lily M; Mardiati, Yayah
BERITA BIOLOGI Vol 8, No 3 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i3.792

Abstract

An experiment was carried out to find out the optimum nitrogen and phosphorous concentration for growth and phycocyanin production in Spirulina fusiformis culture. The cultures were grown in Zarouk medium at various nitrogen and phosphorous concentrations, which were 0.0 mM N, 7.5 mM N, 15.0 mM N, 22.5 mM N, and 30.0 mM N, as well as 0 raM P, 90 mM P, 180 mM P, 270 mM P, and 360 mM P, with four replications each. The result shows that optimal growth of the alga, which is expressed in terms of the biomass yield, was achieved at nitrogen and phosphorous concentration of 7.5 mM and 270 mM, respectively.At the same time, the highest phycocyanin content was obtained at nitrogen concentration of 22.5 mM, which was 1,2% of the biomass, and phosphorous concentration of 360 mM, which was 1.1% of the biomass. According to this result, it is suggested the optimum concentration of nitrogen and phosphorous in the media of Spirulina culture for phycocyanin production is 22.5 mM and 360 mM, respectively.
GROWTH AND PHYCOCYANIN PRODUCTITIVITY OF SPIRULINA FUSIFORMIS UNDER VARIOUS LIGHT REGIMES Chrismadha, Tjandra; Waluya, Rizky Agus
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9097.544 KB) | DOI: 10.14203/ann.bogor.2013.v17.n1.1-6

Abstract

A blue green alga Spirulina fusiformis was grown under various light regimes in a laboratory scale experiment. A 500 watt halogen lamp was employed as the light source, while the light variation of 2,000 lux, 4,000 lux, 6,000 lux, 8,000 lux, and 10,000 lux was obtained by placing a series of 2 L experimental bottles at various distance. The growth medium used was modified Zarrouk medium with initial pH of 8.72, and room temperature was 28-30oC. After inoculation the alga was let to grow for 30 days, and observation on the biomass, chlorophyll, crude protein, and phycocyanin content were carried out every 10 days. The algal biomass was determined gravimetrically, the chlorophyll, phycocyanin, and protein content were measured using spectrophotometer after extraction in 90% aceton, pH 7.0 buffered water, and folin-phenol dye binding, respectively. The result shows a remarkable effect of light intensity to the algal biomass as well as the biochemical content. The specific growth rate increased from 0.08 doubling/day at light intensity 2,000 lux to 0.14 doubling/dat at 10,000 lux, which was equivalent to an increase in the biomass productivity of more than 3 times. The highest algal chlorophyll content was observed at light intensity between 4,000-8,000 lux, indicating the optimum light condition at that irradiance range. The protein content was consistently lower with light intensity, from 42.96-52.91% DW at 2,000 lux to 33.71-41.08 % at 10,000 lux. A consistent drop in the protein content also observed with the culture growth phase, from 39.82-52.91% DW in the early growth stage down to 33.71-42.96% at day 30. Light intensity in concomitance with the growth phase remarkably increase the algal phycocyanin content. In the early growth stage the phycocyanin content was ranged from 0.16% DW at 2,000 lux up to 1.229% DW at 10,000 lux, whereas at the end of the experiment the algal phycocyanin content were 1.04% DW and 2.436% DW, at 2,000 lux and 10,000 lux, respectively. It gave a consequence of more than 7 times higher phycocyanin productivity, which was from 0.09 mg/L/day at 2,000 lux to 0.62 mg/L/day at 10,000 lux. This result shows the importance of light factor in producing phycocyanin from the blue green alga Spirulina fusiformis.