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KONDISI OPTIMUM UNTUK PRODUKSI KITINASE DARI STREPTOMYCES RKT5 DAN KARAKTERISASI PH DAN SUHU ENZIM Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.446 KB) | DOI: 10.24002/biota.v13i3.2571

Abstract

Chitinase is chitin degrading enzyme which is produced by Streptomyces Rkt 5 is isolated microorganism from peanut rhizosfer. This enzyme and its microorganism can be used in many agricultural, medicine and industrial purposes. The aim of the research was to find out the optimum condition for production of chitinase and to characterize of pH and temperature to chitinase activity. Optimalizing production the research had 4 treatments. The optimum conditions were achieved at mineral liquid medium containing with chitin 0,2% (w/v) as inducer, 10% (v/v) inoculum, pH 7 and 48 hours incubation. The crude enzyme was partially purified by salting out with 70% ammonium sulfate resulted in 3.31 time more purity enzyme than the crude one. This enzyme had maximum activity at 50oC and pH 5.5.
KEMAMPUAN KITINASE STREPTOMYCES RKT5 SEBAGAI ANTIJAMUR TERHADAP PATOGEN FUSARIUM OXYSPORUM Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.537 KB) | DOI: 10.31258/jnat.14.1.42-46

Abstract

The purpose of the reasearch is to determine of antifungal activity from chitinase from Streptomyces RKt5 to inhibite growth of Fusariumoxysporum. The chitinase of Streptomyces RKt5 produced in liquid chitin medium with optimum conditions (inoculum concentration, pHand incubation time) and then partially purified with ammonium sulphate. The enzyme products were tested the antifungal activity againstF.oxysporum. The results showed that mycelial growth of F.oxysporum can be inhibited by Streptomyces RKt 5 in dual culture test. Thepartial purified chitinase enzyme couldn?t inhibit the fungal growth. But if the mycellium fragmented, the enzyme could degrade the fungalcell wall in incubation time. The frequency of fungal cell wall lysis and levels of N-acetylglucosamine released that have been increasingalong with the length of incubation time.
EKSPLORASI DAN BIOASAI BERBAGAI ISOLAT BACILLUS THURINGIENSIS LOKAL TERHADAP LARVA BEBERAPA JENIS SERANGGA Suryanto, Dwi; Chairani, Chairani; Rusika, Deddy; Lubis, Nita A.; Yurnaliza, Yurnaliza
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.757 KB) | DOI: 10.24002/biota.v12i1.2538

Abstract

Nine isolates of Bacillus thuringiensis of North Sumatra have been isolated from soil and dead larvae of Plutella xylostella. Bioassay of these isolates to larva of Heliothis armigera, Plutella xylostella, Aedes aegypti, and Culex sp. showed that the isolates have different spectrum and ability in controlling insect larvae. Interestingly, TU1 has similar ability to that of Dipel (B. thuringiensis var kurstaki strain HD-7) in controlling Dipteran (A. aegypti and Culex sp.) and Lepidopteran (H. armigera and P. xylostella). The others were not effective to Dipteran, but have various abilities to kill Lepidopteran. This result indicated that local isolates of B. thuringiensis of North Sumatra were varied in ability to kill different larvae.
EFEKTIVITAS BERKUMUR LARUTAN EKSTRAK ETANOL KULIT KAYU MANIS DALAM MENURUNKAN AKUMULASI PLAK GIGI Waty, Syahdiana; Suryanto, Dwi; Yurnaliza, Yurnaliza
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol 12 No 1 (2017): Jurnal Ilmiah PANNMED Periode Mei - Agustus 2017
Publisher : Politeknik Kesehatan Kemetrian Kesehatan Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.175 KB)

Abstract

Kulit kayu manis merupakan salah satu obat tradisional yang diduga mengandung beberapa senyawa antibakteri seperti flavonoid, saponin dan cinnamaldehid yang dapat menghambat pertumbuhan bakteri di rongga mulut. Efek ekstrak kulit kayu manis secara in vitro terhadap bakteri di rongga mulut telah sering dilakukan namun pengaruhnya sebagai obat kumur dalam menurunkan indeks plak gigi perlu dikaji. Penelitian ini bertujuan untuk mengetahui efektivitas berkumur dengan larutan ekstrak etanol kulit kayu manis dalam menurunkan akumulasi plak gigi. Kulit kayu manis diekstrak secara maserasi dengan etanol 80%. Obat kumur kulit kayu manis dibuat pada konsentrasi 6.25%. Sampel dalam penelitian ini merupakan responden yang berkunjung di klinik Drg. Syahdiana Waty, Medan, Sumatera Utara. Pengukuran indeks plak gigi dilakukan dua kali yaitu sebelum berkumur dan sesudah berkumur dengan larutan ekstrak etanol kulit kayu manis. Analisis data yang digunakan adalah uji Wilcoxon untuk mengetahui perbedaan yang signifikan antara indeks plak gigi sebelum berkumur dan sesudah berkumur dengan larutan ekstrak etanol kulit kayu manis. Hasilnya menunjukkan bahwa ekstrak etanol kulit kayu manis mengandung senyawa metabolit sekunder golongan alkaloida, flavonoid, saponin dan glikosida. Perbedaan indeks plak gigi yang signifikan antara sebelum dan sesudah berkumur dengan larutan ekstrak etanol kulit kayu manis 0.000 (<0.05). Berdasarkan hasil penelitian ini dapat disimpulkan bahwa larutan ekstrak etanol kulit kayu manis efektif menurunkan akumulasi plak gigi.