Budijono, Budijono
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ISOLATION AND IDENTIFICATION OF BIOSURFACTING BACTERIA FROM THE CONTACT POND OF WWTP PALM OIL INDUSTRY POND Sembiring, Raynaldo Empindonta; M. Hasbi, M. Hasbi; Budijono, Budijono
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractBiosurfactants are surfactants produced by bacteria capable of degrading oil in water. This study aims to obtain bacteria that can produce biosurfactants from the Contact Pond of IPAL palm oil industry PT. Eka Dura Indonesia, Kota Lama, Rokan Hulu Regency, which was held from April to July 2019. The procedure for the implementation, first, the sample was diluted and enriched using BHI (Brain Heart Infusion) media for 24 hours. Based on the results of the study found 3 isolate of bacteria. The three bacteria are bacteria that can produce bio surfactants with an emulsification index of Isolate D1 41.81%, Isolate D2 43.6%, and Isolate D3 32.72%. Keywords: Bio surfactants, Proteus vulgaris, Proteus mirabilis, and Enterobacter aerogenes 
ISOLATION AND IDENTIFICATION OF BIOSURFACTANT PRODUCING BACTERIA FROM CONTACT POND PONDS OF PALM OIL INDUSTRY WWTP Br Sembiring, Elma Damayanti; M. Hasbi, M. Hasbi; Budijono, Budijono
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract Biosurfactants are surfactant compounds produced by microorganisms, and have the ability to reduce surface tension, and can break oil. This study aims to obtain biosurfactant producing bacteria from the pond contact ponds of the palm oil industry WWTP in PT. Sawit Asahan Indah, Ujung Batu, Rokan Hulu Regency. This research was conducted in March-May 2019. First, samples were enriched and diluted with BHI media for 24 hours. Then bacteria are identified by biochemical methods and 18S rDNA. The results revealed that the total number of colonies was 8.7 x 106 Cfu / ml. Morphological and biochemical characteristics obtained 3 types of bacteria, namely Proteus vulgaris, Proteus mirabillis, and Escherichia coli. Two of them are biosurfactant bacteria with the Proteus vulgaris emulsification index 58.3%, and Proteus mirabillis 61.6%. Keywords: Biosurfactant, Proteus vulgaris, Proteus mirabilis, Emulsification Index, 18S rDNA, Biodegradation
THE EFFECT OF BIORAFIA DENSITY ON DECREASING NITRATE ON THE SAIL RIVER Sitompul, Dorlan Rotua; Budijono, Budijono; M.Hasbi, M.Hasbi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT The Sail River has been contaminated with nitrate can be reduced by using biorafia (interweaving of several raffia knot masks that are mashed with shapes resembling duster as a place to attach to microorganisms). This study aimed to determine the effect of the density of biorafia in reducing levels of nitrate contained in the water of the Sail River. The study was conducted in March-April 2019 on the Sail River. This study uses a Completely Randomized Design (CRD) with a single factor, namely biorafia. Using three treatments, namely different densities (P1 = infrequent / 50simple, P2 = moderate / 75impul and P3 = meeting / 100impul), and three replications. Sampling was carried out before and after passing biorafia on artificial trenches with a one-month interval. The results showed that biorafia was able to reduce the levels of nitrate with a decrease in average not much different. The decrease in nitrate ranges from 0.1375-0.33473 mg / L to 0.0750-0.1167 mg / L (35-44%). Biorafia can be used in reducing levels of nitrate. Keywords: Biorafia, Nitrate,raffia
The EffectivenessOf BiorafiaIn Reducing Biological Oxygen Demand (BOD5) Content In The Sail River’s Water Andini, Rizka Novtri; Budijono, Budijono; Purwanto, Eko
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTOne of the pollutions that enters the Sail River is organic pollutants derived from domestic waste. Efforts to reduce organic pollutants can be conducted by using biocords. Biocord is a rope in the form of three-dimensional fine fiber webbing that is able to hold microorganisms in large quantities. This research used biorafia media (material made from raffia-based materials). The method used was CRD with 3 different biorafia densitieslow density (P1), medium density (P2) and  high density (P3) with 3 replications each. Biorafia is placed in a ditch drained from the Sail River. Biorafia functions as a place attached to microorganisms (biofilms). The degradation process of organic matter occurs when in contact with biofilms that are present in biorafia. The purpose of this study was to reduce the levels of BOD5. The results showed a decrease in BOD5 levels with a time interval of 1 month. The effectiveness of the decrease in the average BOD5 was P1 71,4,%, P2 74,1% dan P3 69,5%. Keywords:biocord, biorafia, organic pollutants, microorganisms 
Pb and Znconcentration in the water, sediment and Tubifex sp living in the Sail River, Pekanbaru Yohana R, Desita; M. Hasbi, M. Hasbi; Budijono, Budijono
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractSail River is one of polluted rivers in Riau. High Pb and Zn concentrations in the water and sediments may affects organisms living in the  rivers, including Tubifex. A study aims to understand the Pb and Zn concent in the water, sediment and Tubifexin that river was conducted in October - November 2017. There were 3 stations, in the upstream (St1), in the residential area (St2) and in the downstream (St3). Samplings were conducted 3 times,once/2 weeks. Results indicate that the concentration of Pb in the water, sediment and  Tubifex were 0.276 to 0.776 mg/L, 1.249 to 3.643 mg/kg and 3.643 to 5.613 mg/kg. While the Zn concentration in the water, sediment and  Tubifex were 0.597 to 0.785 mg/L, 62.165 to 124.329 mg/kg and 83.982 to 94.995 mg/kg respectively.  It was concluded that the water, sediments and Tubifex worms from the Sail River were already contaminated with Pb and Zn.Keywords :  Heavy metals, polluted rivers, residential area, contaminated
The Effectiveness of Biorafia in Reducing Total Suspended Solid Content in the River Sail Water Fradillah.S, Nailul; Budijono, Budijono; M. Hasbi, M. Hasbi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractSail River is one of the Siak watersheds which has high TSS levels. This study aims to determine the decrease in TSS levels using the different density of biorafia (plastic rope that functions as a filter). This research was conducted on the Sail River, from March to April 2019. The method used was CRD with 3 different biorafia densities: P1 (low density), P2 (medium density) and P3 (high density), with 3 replications. Sampling is done before passing the biorafia (inlet) and after passing through the biorafia (outlet). The highest reduction in TSS levels was found at moderate density (190 mg / L) with a decrease in effectiveness (EP) of 96,9%. The density of different biorafia does not significantly influence the decrease in TSS levels in the Sail River water. Keywords : Biocord, Biorafia, Suspended Solid and Bacteria
Isolation And Identification Of Biosurfactant Bacteria In Anaerobic Pond Wastewater In Palm Oil Industry WWTP Yolanda, Nadia; Hasbi, Muhammad; Budijono, Budijono
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
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AbstractPalm oil waste can have a negative impact on the environment and can stimulate gene mutations in certain bacteria. The bacteria can produce bio surfactants to reduce the level of oil pollution. This study aims to identify bacteria capable of producing bio surfactants from the anaerobic pond of the palm oil industry WWTP PT. Sawit Asahan Indah, Ujung Batu, Rokan Hulu Regency. The study was conducted from April to May 2019. Samples were diluted and enriched using BHI (Brain Heart Infusion) media for 24 hours. Furthermore, samples were identified in morphological, biochemical and RNA (18SR) methods. Based on the results of the study, three types of bacteria were obtained, namely Proteus Mirabilis, Proteus vulgaris and E. coli. The Proteus Mirabilis emulsification index is 46.6%, and Proteus vulgaris is 45%. This shows that all bacteria found are able / potentially produce bio surfactants. Keywords:palm oil pollution, bio surfactant, Proteus vulgaris, Proteus mirabilis, emulsification index, RNA (18SR) method, Biodegradation.
GROWTH OF Chlamydomonas sp. AS A PRELIMINARY STEP ON THE METAL TOXICITY TESTING Siahaan, Yoshua Nobel; Budijono, Budijono; Hindarti, Dwi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
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ABSTRACT             This study is aimed to learn the capabilities of Chlamydomonas sp. as biota qualifies for metal toxicity test. This research is done for 17 days by seeing an entire living phase of Chlamydomonas sp. in the growth curve. A cell density analysis from Chlamydomonas sp. calculated using a microscope.This result indicate that there are 4 living phases of Chlamydomonas sp. it?s composed of phases lag, an exponential phase, stationary phases and phases leading to death. Chlamydomonas sp. qualified as a test biota for a heavy metal toxicity test using phytoplankton according to Asean-Canada Cooperative Programme on Marine Science Phase II. This is seen from the growth of cell density that exceeds the minimum limit on the 4th day of inoculation growth curve or at the beginning of an exponential phase with density of cell 3,68 x 104 cell.mL-1.Kata Kunci : Phytoplankton, Chlamydomonas sp., Toxicity test
THE EFFECT OF BIOJUK TREATMENT ON DECREASING TOM AND BOD LEVELS CONTAINED ON RIVER SAIL WATER IN PEKANBARU CITY Putriningsih, Hawari; Budijono, Budijono; M. Hasbi, M. Hasbi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
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ABSTRACTSail River is one of the rivers in the city of Pekanbaru that has been polluted with organic pollutants. This study aims to determine the decrease in TOM and BOD levels by using different bioijuk densities. This research was carried out on the Sail River, in March-April 2019. The method used was an incomplete design (CRD), with 3 treatments of bioijuk density namely P1 (low density), P2 (medium density) and P3 (high density), and each -one with 3 replications. The sampling location was selected in the inlet section (before passing bioijuk) and the outlet section (after passing bioijuk) artificial ditch. The results showed that the highest decrease in TOM levels occurred in P2 (0.046 mg / L) with a decrease in effectiveness (EP) of 95%. The highest decrease in BOD levels was found in P2 (4.66 mg / L) with an EP of 53%. It turns out that the treatment of different bioijuk densities does not have a significant effect on decreasing levels of the TOM and BOD of the Sail River.Keywords: Bioijuk, TOM, BOD, bacteria, effective sperm
The Effect Of Biorafia Fiber Density On The Decrement Of Pb And Cr In Sail River’s Water Irde, Mira Kurnia; Budijono, Budijono; Purwanto, Eko
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTOne of the pollutants that insert to the Sail River is a heavy metal. The effort that will the researcher do to reduce the level of heavy metal (Pb and Cr) is using the biocord. Biocord is a rope from the three-dimension of woven fine fiber that can hold the microorganisms in a large quantity. This research uses the raffia media so that this research names biorafia. This research aims to determine the effect of biorafia density toward the decreasing level of heavy metal such as Pb and Cr on the water of the Sail River. This research has done in March - April 2019. In this research, the researcher uses a completely randomized design (CRD) method using 3 treatment levels, and 3 replications. 3 treatment levels of biorafia density are P1 (rare), P2 (moderate), and P3 (dense). The result shows us a decreasing level of heavy metal such as Pb and Cr. The decreasing values of the heavy metal level of Pb are P1 (0.071 mg / L), P2 (0.074 mg / L), and P3 (0.073 mg / L). Also, the decreasing values of the heavy metal level of Cr are P1 (0.055 mg / L), P2 (0.056 mg / L), and P3 (0.055 mg / L).Keywords:Biorafia density, heavy metals, lead, chromium