Purwantiningsih, Purwantiningsih
Unknown Affiliation

Published : 3 Documents
Articles

Found 3 Documents
Search

Major Metabolites of Methylripariochromene-A, a Bioactive Substance in The Leaves of Eupatorium Riparium Reg. in Male Rats Urine Wahyuono, Subagus; Vairappan, Charles S.; Mohd., Datin Maryati; Fauziati, Vera; Purwantiningsih, Purwantiningsih; Marchaban, Marchaban
Majalah Farmaseutik Vol 14, No 1 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.007 KB) | DOI: 10.22146/farmaseutik.v14i1.41928

Abstract

Methylripariochromene-A (MRC) (1) is the principal bioactive compound isolated from Eupatorium riparium Reg. collected from the Mt. Merapi National Park. MRC was isolated from the chloroform extract of the leaves by using bioassay (BST) guided isolation method. MRC causes diuretic, reduces the blood pressure and excretes mineral from the body. This activity is comparable to a clinically used diuretic drug, furosemide. MRC is a chromene type of compound, having 2 ?OCH3 groups on the aromatic ring that are possibly demethylated in vivo to give phenolic compounds well known for having antiseptic properties. This study was aimed to evaluate diuretic properties compared to furosemide, isolate and identify MRC major metabolites present in urine. If it is true, then MRC probably can be used also as anti-infectious diseases in the tractus urinary. n-Hexane extract (81% MRC) of E. riparium (1.25 g/kg bw) was applied p.o on male rats (+ 200g) and furosemide was used as a positive control of diuretic activity. Urine was collected during 48 hours, partitioned with EtOAc for 15 minutes to give EtOAc fraction at the upper layer. The EtOAc fraction was then checked by tlc with MRC as the reference standard. The major metabolites were isolated by fractionating the EtOAc fraction followed by purification using preparative tlc (fr. 4-5 and 6-7). The major metabolites obtained were identified based on their 1H- and 13C-NMR data. Diuretic effect of n-hexane extract (1.25 g/kg bw) was comparable to furosemide (8.83 mL/rat vs 8.70 mL/rat for 24 hours). The 1H- and 13C-NMR data of fr 4-5 indicated that there was not major changing in the 1H- and 13C-NMR spectra appearance compared to that of MRC, except that 1 ?OCH3 group was demethylated became demethyl MRC (2) . Based on HMBC and HMQC experiments, -OCH3 at C-8 was the one demethylated. The 1H-NMR data of fr. 6-7 was similar to fr. 4-5, demethylation of one ?OCH3 group on C-8 but the ether linkage in the furan ring system was broken to give 2 tentative structures (3 and 4).
ESTIMASI ALIRAN SUNGAI BAWAH TANAH DI DAERAH DENGOK DAN NGREJOK WETAN GUNUNG KIDUL, MENGGUNAKAN METODE VLF-EM DAN VLF-EM-VGRAD Anita, Febria; Purwantiningsih, Purwantiningsih
Sainstek : Jurnal Sains dan Teknologi Vol 9, No 2 (2017)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.934 KB) | DOI: 10.31958/js.v9i2.607

Abstract

A baseline river flow estimation has been conducted in the area of Duncok and Ngrejok Wetan Gunung Kidul, using VLF-EM and VLF-EM-VGRAD methods to find out VLF response of tilt angle mode. Interpretation is done from VLF and VLF-EM-VGRAD data of tilt angle and ellipticity to estimate underground stream that is interpreted qualitatively and quantitatively. Measurements were made on July 22-26, 2014. The data were processed by using uses MATLAB R2014a computational language. This qualitative interpretation is done by processing data filtered by Moving Average through Karous-Hajelt filter and Fraser filter so as to show the anomaly of underground conductive object. Of the two methods used, the most effective method is with VLF EM, it can be seen from the result of the Karous Hjelt filter shows the contrast resistivity in VLF EM compared to VLF-EM-Vgrad
ANALISIS SEBARAN DOSIS PAPARAN RADIASI PESAWAT C-ARM TERHADAP JARAK PADA RUANGAN OPERASI Purwantiningsih, Purwantiningsih
Sainstek : Jurnal Sains dan Teknologi Vol 9, No 2 (2017)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.884 KB) | DOI: 10.31958/js.v9i2.1152

Abstract

Analysis Of Radiation In The Operating Room From C-Arm Radiation By Thermoluminiscence Dose (TLD).Measurement of C-arm radiation exposure in the surgery room is very important to know the amount of radiation scattered, so that we can ascertain the amount of radiation received by the doctor, nurse or radiology operator in the room. C-armRadiation dose was meansured in the surgery room at Awal Bross Hospital, Bekasi, by using Thermoluminenscence Dosimeters. Radiation dose in the operation room was meansured at 12 points in the room and 1 point at the center of the room. From this study, the highest radiation dose is at the center of the room, 1,021 mSv. The maximum radiation dose at 1 meter from the center is 0,047 mSv and the minimum dose is 0,013 mSv. The maximum dose at 2 metrs from the center of the room is 0,039 mSv and the minimum dose is 0,028 mSv. This all result is still in dose limit value for one examination.