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PENAPISAN BEBERAPA PADI LOKAL DARI PULAU ENGGANO TERHADAP KETAHANAN SALINITAS Astuti, Dwi; Nurhasanah, Ade Nena; Nugroho, Satya; Estiati, Amy
Jurnal Lahan Suboptimal: Journal of Suboptimal Lands Vol 6, No 2 (2017): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.544 KB) | DOI: 10.33230/JLSO.6.2.2017.282

Abstract

Astuti et al, 2017. Screening of Several Local Rice from Enggano Island to Salinity Tolerance. JLSO 6(2):134-141. Enggano Island, Bengkulu Province, has a lot of local rice germplasm that has been cultivated by people in Enggano Island that is more than 30% as farmer. Identification of local rice from Enggano Island against biotic and abiotic stresses, especially salinity stress is necessary. The identification can be used, among other things, as the information of the local rice planting that related to the rising sea level in Enggano Island which impact on the condition and the area of rice cultivation land, and as a source of finding for resistance genes against salinity stress for improvement of those local rice varieties. Aim of this study is preliminary screening of some local rice from Enggano Island to salinity stress in seed and germination stages. The analysis used Factorial Randomized Design, two factors, the first factor  is salt concentration and and the second is local variety. The salt concentrations used were 0 millimolar NaCl, 250 millimolar NaCl, NaCl 275 millimolar and NaCl 300 millimolar and the rice sample used were 11 local rice of Enggano Island, NiponBare varieties as control of rice plants, Pokali as resistant control and IR29  varieties as susceptible controls. Rice seeds that used in this test are seed in seed stage and germination stage of 10-14 days old. This preliminary screening shows three local genotype of Enggano Island that tolerance to Salinity.
Overexpression of OsHox-6 Gene Enhanced Tiller Number in Rice But Induced Yield Penalty Rahmawati, Syamsidah; Chairunnisa, Chairunnisa; Erdayani, Eva; Nugroho, Satya; Estiati, Amy
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (712.857 KB) | DOI: 10.14203/ann.bogor.2019.v23.n1.30-40

Abstract

OsHox-6, belongs to the transcription factor homeodomain leucine zipper (HD-Zip) protein sub-family I, has unknown function. This study was aimed to characterize the phenotypes of two homozygous transgenic rice lines (S29-62-2 and S.40.4-158-1) containing an extra copy of OsHox-6 gene under the control of a rice constitutive promoter, OsLEA3, and to evaluate their tolerance to water stress. A real-time quantitative PCR (qRT-PCR) showed that the transcript expression of OsHox-6 gene in the transgenic lines increased 5-10 folds under a normal irrigation and 10-20 folds after exposure to water stress conditions as compared to its wild type control. Transgenic plants overexpressing OsHox-6 exhibited phenotypic alteration at the normal irrigation by inducing tiller formation, suggesting a decrease in the apical dominance. Transgenic plants also showed significant enhancement in the total grain number, however, the number of empty grains  also increased significantly (~16-22%).  After imposed to the water stress, the number of empty grains in the transgenic lines was even higher (up to 83% in average). Furthermore, observations on the water loss rates, relative water contents and drought resistance indices (DRI) suggested that the overexpression of OsHox-6 did not significantly increase tolerance to water stress.  Further research is required to reveal the detailed mechanisms of OsHox-6 in response to water and other abiotic stresses.