Krishanti, Ni Putu Ratna Ayu
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PRODUCTION OF CONIDIA BY ENTOMOPATHOGENIC FUNGI AND THEIR PATHOGENICITY AGAINST COPTOTERMES SP. Zulfiana, Deni; Zulfitri, Apriwi; Lestari, Anis Sri; Krishanti, Ni Putu Ratna Ayu; Meisyara, Dita
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22435

Abstract

Entomopathogenic fungi have the potential to infect most arthropods including termites which are economically important major insects pest of wood, wood products and building structures. However, the application of this fungus in the field has not shown satisfactory results yet, one of which is constrained in mass production of conidia. The purpose of this study was to evaluate 16 types of biodegradable products and waste as substrates for mass production of conidia using solid state fermentation method and two types of inoculum namely solid and liquid inoculum. Toxicity tests were carried out on subterranean termites (Coptotermes sp.) based on JIS K 1571, 2010. The parameters observed were the number and dry weight of the conidia produced, conidial viability, nutrient content of the substrate, and percentage of termite mortality. The results showed that rice, sorghum and corn were the best media for the growth of entomopathogenic fungi based on the number of conidia and dry weight of the conidia produced. Metarhizium sp. T4.B23 produced the highest number of conidia, 1.12 x 1011 conidia/100 g substrate and yielded 180.9 ± 0.623 g dry conidia/kg of rice; followed by Metarhizium sp. B2.2 grown on sorghum that resulted in 1.11 x 1010 conidia/100 g substrates and 8 ± 0.570 g /kg sorghum; and B. bassiana produced 8.3 x 109 conidia/100 g substrate and 31.24 ± 0.407 gr/ kg sorghum. Metarhizium sp. B2.2 showed the highest toxicity to termites with 100% mortality was observed within the second day of testing. Therefore the conidia of Metahizium sp. B2.2 is potential to be developed as a biopesticide using rice or sorghum substrate as a carrier.
Biological Control of Wood Destroying Organism Using Plant Extracts Collected from Mt. Merapi National Park, Indonesia Ismayati, Maya; Zulfiana, Deni; Tarmadi, Didi; Lestari, Anis Sri; Krishanti, Ni Putu Ratna Ayu; Himmi, Setiawan Khoirul; Fajar, Anugerah; Yusuf, Sulaeman
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20102

Abstract

Due to the climate change and global warming, the biodiversity database has gained the attention of the government. In line with the Indonesian Biodiversity Strategy and Action Plan (IBSAP), we have collected plants with insecticidal activity based on the local wisdom. This program aimed to protect Indonesian biodiversity from deforestation along with the loss of the number of species. This research?s goal was to evaluate termiticidal and antifungal properties from some plant extracts collected from the Turgo forest area, Mt. Merapi National park, Java. Three potential plants were evaluated. Based on specimen identification, the three plant samples were Kina (Cinchona sp.), Kamadoh (Dendrocnide stimulans (L.f.) Chew), and Keremi (Homalanthus populneus (Geiseler) Pax). The phytochemical test showed that Kina contained alkoloid, falvonoid, saponin, and tannin, whereas Kamadoh and Keremi contained saponin and tanin, respectively. Overall, all plant extracts have the termiticidal activities and able to inhibit wood- decay fungi with the inhibition percentage around 60% - 100%. Leaf extracts of Kina (Cinchona sp) and Keremi (Homalanthus populneus (Geiseler) showed the highest activity as wood-decay fungi inhibitor. The disclosure of the potential of bioinsecticides from some plants originating from Mount Merapi is very important before being lost due to deforestation and the Mount Merapi disaster. Thus, the potential bioinsecticide in these plants can increase its economic value as a substitute for synthetic insecticides that are friendly to the environment.