Articles

Found 1 Documents
Search

IMMUNOPROTECTIVE EFFECT OF MELATONIN ADMINISTRATION ON METHYLPREDNISOLONE-TREATED WISTAR MALE RAT (RATTUS NORVEGICUS) Manus, Widya Christine; Pangkahila, Wimpie; Aman, I Gusti Made
Berkala Ilmiah Kedokteran Duta Wacana Vol 4, No 2 (2019): Berkala Ilmiah Kedokteran Duta Wacana
Publisher : Faculty of Medicine Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/bikdw.v4i2.150

Abstract

Background: Immune system function decreases with age. Steroid use might accelerate this process, especially in adults in Indonesia. Melatonin is a natural hormone which show antioxidant effect and immunoprotective effect. Melatonin administration might help in reviving humane immune system due to aging and exogenous steroid exposure. Objective: This experiment is aimed to determine the effect of melatonin on leukocyte and lymphocyte count of methylprednisolone-induced immunosuppression on Wistar rat. Method: This study was true experimental with post-test only control group design using 32 male rats. Rats were divided into control and the intervention group. Control group was given methylprednisolone 0.144 mg/200 g rat 3 times a day and distilled water. The treatment group was given methylprednisolone 0.144/200 g rat 3 times a day and 0.054 mg/200 g melatonin once daily. The blood was taken after 14 days of treatment. Results: Intervention group shows higher lymphocytes count (4.95 ±1.58x103/?L) compared to the control group (3.09?1.33x103/?L), with p < 0.01. The intervention group also has higher leukocyte count (8.37± 2.02x103/?L) compared to the control group (6.70 ± 1.96x103/?L), with p