Sobir ,
Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti Kampus IPB Darmaga, Bogor 16680, Indonesia

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PENGEMBANGAN KRITERIA SELEKSI UNTUK PERAKITAN TERUNG (SOLANUM MELONGENA L.) BERDAYA HASIL TINGGI Putri, Faradila Danasworo; ,, Sobir; Syukur, Muhamad; Maharijaya, dan Awang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 2 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.173 KB) | DOI: 10.24831/jai.v45i2.13077

Abstract

Eggplants (Solanum melongena L.) are consumed worldwide in a variety of colors, shapes and sizes, thus vegetable plant breeders place great interest in developing high yielding genotypes in response to the population growth and the need to increase vegetable production. This research was aimed to determine the proper character as selection character for breeding high yielding eggplants by using estimated heritability value, coefficient of genetic diversity, characters correlation and path analysis. The experiment was conducted during 2014 to August 2015 at Cikabayan Experimental Field, Tajur Experimental Field and Pasir Sarongge Experimental Field, IPB, West Java. A total of 12 characters was observed in the 25 genotypes. Eleven characters has a high estimated heritability value and high coefficient of genetic diversity value. Those characters were time of flowering, time of harvest, fruit length, fruit weight, fruit diameter, plant height, stem length, fruit apical scar length, calyx length, the number of fruits per plant and fruit weight per plant. Results of correlation and path analysis showed that fruit length, fruit weight, fruit diameter, stem diameter, calyx length and number of fruits per plant have a direct positive effect on fruit weight per plant. Fruit weight, fruit diameter and number of fruits per plant are suitable characters in determining the selection criteria for developing high productivity eggplant.Keywords: path analysis, correlation, heritability, genetic diversity coefficient
IDENTIFICATION OF GENE RELATED TO HARD BUNCH PHENOTYPE IN OIL PALM (ELAEIS GUINEENSIS JACQ.) ,, Roberdi; ,, Sobir; Yahya, Sudirman; Toruan-Mathius, Nurita; Liwang, Tony
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.814 KB) | DOI: 10.24831/jai.v43i2.10421

Abstract

ABSTRACTMolecular genetic analysis of hard bunch phenomenon in oil palm was done in order to elucidate the role of genetic factor underlying hard bunch in oil palm plantation. The aim of this study was to identify the AFLP primer combination that co-segregates with hard bunch phenotype related gene in oil palm. Molecular analysis was done by bulk segregant analysis approach. DNA was isolated from leaves of the normal and hard bunch palm. DNA from ten individual palms from each category were pooled and used as a template. A total of 56 AFLP primer combinations were selected for selection of polymorphic primer, and as a result it was found that 22 AFLP primer combinations (39.28%) were polymorphic. A total of 48 individual of palm DNA containing 24 individual for each group were further genotyped by those 22 polymorphic markers. Of these, one AFLP primer combination (E-ACC/M-CTG) was obtained as a co-segregated marker that distinguished the hard bunch DNA from the normal one. Based on the analysis of the target sequence aligned to the oil palm DNA sequences available in database, we found that our sequence has similarity with Ty-1 copia retrotransposon. This sequence distribute in all 16 linkage group of oil palm genome.Keywords: abnormal fruits, AFLP, oil palm, Ty-1 copia retrotransposon
IDENTIFIKASI SPESIES CABAI RAWIT (CAPSICUM SPP.) BERDASARKAN DAYA SILANG DAN KARAKTER MORFOLOGI ,, Undang; Syukur, Muhamad; ,, Sobir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (687.223 KB) | DOI: 10.24831/jai.v43i2.10413

Abstract

ABSTRACTThe chili consists of several species, five of which have been cultivated, namely C. annuum, C. chinense, C. frutescens, C. baccatum, and C. pubescens. The classifications of these species are based on: 1) morphological characters, especially floral morphology, 2) crossability between species, and 3) fertile hybrids between species. Species C. baccatum and C. pubescens can be easily identified and distinguished from one another, because there are obvious differences in the two species. However the species C. annuum, C. chinense, and C. frutescens has many common characteristics, so it is difficult to distinguish morphologically. The purpose of this study was to identify capsicum species based on crossability and morphological characters. The crossability experiment was done in housing area TDP 2 Ciampea Bogor from January to December 2013 and the morphological characteristics at Leuwikopo experimental station, IPB. The morphological characters experiment was arranged in randomized complete block design (RCBD) with three replications. Data were collected on successful crosses, seed viability of successful crosses and morphological characters based on descriptor for capsicum IPGRI. The percentage of successful crosses involving IPBC10 and IPBC145 (C. annuum) as female parents and 20 genotypes as male parents ranged between 0-90%; and using IPBC295 (C. frutescens) as female parent was 40%. Genotypes which had successful results equals 40% were alleged as C. frutescens species (IPBC61, IPBC139, IPBC63, IPBC163, IPBC289, IPBC288, IPBC294 and IPBC285). Principal component and of clusters analysis suggests that there are two species groups, i.e. C. annuum and C. frutescens.Keywords: capsicum, cluster analysis, crossing, genotypes, principal component analysis
IDENTIFIKASI PENANDA SSR YANG BERASOSIASI DENGAN BOBOT TANDAN BUAH KELAPA SAWIT (ELAEIS GUINEENSIS JACQ.) Yono, Dwi; Wahyu, Yudiwanti; ,, Sobir; Toruan-Mathius, dan Nurita
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.647 KB) | DOI: 10.24831/jai.v45i1.14014

Abstract

Oil palm is a perennial oil crop that contribute the most important source of vegetable oil in the world. Oil palm breeding cycle take a long period, therefore molecular marker-assisted selection (MAS) is required to shorten the selection time. This MAS require the associated marker to desired trait, particularly for yield and the component that as a complex trait and depend on genetic background. The aim of this study was to obtain SSR marker that associated with average of bunch weight (ABW). Plant material was used in this study is Tenera population derived from Deli Dura x AVROS Pisifera. Seven SSR markers from public database were associated to ABW trait. Molecular data analysed for SSR markers profile which consisted of allelic diversity, heterozygosity level, and polymorphism information content (PIC). Association between SSR markers to ABW traits was performed with single marker analysis using one way analysis of variance. The results showed that SSR markers were able to amplify DNA with two to three number of alleles with the average of 2.3 alleles per locus. ABW trait for this population was significantly associated with mEgCIR3428 marker. This marker may be used in the selection of Deli Dura and AVROS Pisifera palm on the next selection cycle.Keywords: Average of bunch weight, AVROS Pisifera, Deli Dura, single marker analysis
INTERAKSI GENOTIPE X LINGKUNGAN HASIL DAN KOMPONEN HASIL 14 GENOTIPE TOMAT DI EMPAT LINGKUNGAN DATARAN RENDAH Dewi, Suprayanti Martia; ,, Sobir; Syukur, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.1 KB) | DOI: 10.24831/jai.v43i1.9592

Abstract

Genotype x environment interaction (GxE) information is needed by plant breeders to assist the identification of superior genotype. Stability analysis can be done if there is a GxE interaction, to show the stability of a genotype when planted in different environments. This study aimed to estimate the effects of genotype x environment interaction on yield and yield components of fruit weight per plant as well as to look at the stability of 14 tomato genotypes at four lowland locations. The study was conducted at four locations, namely Purwakarta, Lombok, Tajur and Leuwikopo. Experiments at each location was arranged in a randomized complete block design with three replications. Stability analysis was performed using the AMMI model. Fruit weight, fruit diameter, number of fruits per plant and total fruit weight per plant characters showed highly significant genotype x environment interactions. Variability due to the effect of GxE interaction based on a AMMI2 contributed by 88.50%. IPBT3, IPBT33, IPBT34, IPBT60 and Intan were stable genotypes under AMMI model.Keywords: AMMI, multilocation trials
ANALISIS GENETIK SIFAT KETAHANAN MELON (CUCUMIS MELO L.) TERHADAP VIRUS KUNING Hermawan, Entit; ,, Sobir; Efendi, Darda
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.511 KB) | DOI: 10.24831/jai.v42i2.8823

Abstract

ABSTRACTResistance to yellow virus (YV) is an important breeding trait in melon. However information regarding genetic inheritance pattern of the character are limited. This study aimed to provide information on genetic control for resistance to YV in melon caused by Tomato Yellow Leaf Curl Virus (TYLCV). Twenty genotypes from three major melon groups (dudaim, cantaloupe, and inodorous) were evaluated using controlled inoculation method. The results revealed that one line, MEV1 from the dudaim group, showed high resistance to YV; while other lines belong to cantaloupe and indororus indicated as highly susceptible lines. Screening of the F1 from crossing between resistant and susceptible parents resulted in resistant F1 after inoculation and planted in endemic location. Subsequently, evaluation on F2 population revealed a non-normal distribution for disease severity score, indicating that resistance to YV in melon was controlled by major genes. Chi-square (?2) test resulted in 13:3 ratio and indicated that the resistance to YV was controlled by 2 genes pair with dominant and epistasis recessive actions.Keywords: dominant and epstasis recessive action, major gene, Bemicia tabaci, Begomovirus
KERAGAMAN MORFOLOGI DAN KANDUNGAN TANIN PADA TANAMAN LEUNCA [SOLANUM NIGRUM (L.)] Nadila, Dea; ,, Sobir; Syukur, dan Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.751 KB) | DOI: 10.24831/jai.v47i1.19554

Abstract

Black nightshades [Solanum nigrum (L.)] is an indigenous vegetable originatied from tropical and subtropical regions. In Indonesia, black naightshades widely spread in Java, especially West Java. Black nightshades has a bitter taste caused by the content of tannins. The objective of this study was to identify and analyze the morphological and tannin content of 20 black nightshades accessions. The research was started with germplasm exploration in November 2015-February 2016, followed by planting and analysis until September 2016 at a laboratory of the Center for Tropical Horticultural Studies (PKHT) IPB. The experiment was arranged in a single-factor randomized complete block design with three replications. The results from cluster analysis based on morphological variability showed that the accessions of black nightshades can be grouped into two groups. The accessions had different fruit tannin contents, and some accessions can be selected for breeding material.Keywords: group analysis, indigenous vegetable, qualitative character, quantitative character