Kurniasih .
Bagian Patologi Fakultas Kedokteran Hewan UGM

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PELACAKAN GEN AEROLYSIN DARI AEROMONAS HYDROPHILA PADA IKAN MAS YANG DIBERI PAKAN EKSTRAK BAWANG PUTIH (DETECTION OF AEROLYSIN GEN FROM AEROMONAS HYDROPHILA IN COMMON CARP FED WITH GARLIC EXTRACT) Lukistyowati, Iesje; ., Kurniasih
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Aeromonas hydrophila is a gram negative and opportunistic bacteria, which could cause fish mortalityin a short time from 80%-100%. One virulent factor of A. hydrophila on common carp (Cyprinus carpio L)that could cause fish mortality is aerolysin. This research used a synthetic primers of oligonukleotide todetect aerolysin, a specific genomes of A. hydrophila on common carp (Cyprinus carpio L). The commoncarps have been feed a woof that contain garlic extract during 30 days before they challenged with A.hydrophila. Polymerase Chain Reaction (PCR) was used to detect an aerolysin gen from A. hydrophila. Theelectrophoresis result showed aerolysin gene of Aeromonas hydrophila from Veterinary Faculty of GadjahMada University (FKH-UGM) isolate was amplified with 462 bp of molecule weight. While the aerolysingen was detected in the fish kidney with 900 bp of molecule weight. Further, DNA sequence analysis of thePCR product of A. hydrophila from FKH ? UGM isolate showed homolog with isolate A. hydrophila subsphydrophila ATCC 7966 complete genome with score 55.4 (71%).
STUDI RESPON IMUN PADA AYAM YANG DIIMUNISASI DENGAN ANTIGEN EKSKRESI SEKRESI SPOROZOIT EIMERA TENELLLA Prastowo, Joko; Nurcahyo, Wisnu; ., Kurniasih; ., R. Wasito
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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HISTOPATOLOGIS DUGAAN EDWARDSIELLA TARDA SEBAGAI PENYEBAB KEMATIAN IKAN MASKOKI (CRASSIUS AURATUS) : POSTULAT KOCH Ratnawati, Atik; Purwaningsih, Uni; ., Kurniasih
Jurnal Sain Veteriner Vol 31, No 1 (2013): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4537.304 KB) | DOI: 10.22146/jsv.3259

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ABSTRACT              The aim of the study was to know the influence experimental infection of Edwardsiella tarda  was isolated from sick koi fish (Cryprinus caprio) to the histologic features of liver, kidney, gill, brain, intestine and muscle in Goldfish (Carassius auratus) based on Koch Postulate. Three goldfishes (KK) were used in this reseach. KK was infected by Edwardsiella tarda as much as 0.1ml i.m.. KK1 died on the 4th day and KK2 and KK3 died on the 25th day. All the fishes were then necropsied. The livers, kidneys, gills, brains, intestines and muscles were taken for making the histopathologic preparation. Bacteriologic observation of KK1 was conducted by cultivating the bacteria into Brillliant Green Agar (BGA), Triple Sugar Iron Agar (TSI Agar), Urea Agar, Motility Agar, Peptone Broth, sugars and oksidation test.      Result of histopathologic observation showed that there was necrosis, congestion and macrofag limpocytes and neutrophisl infiltrations in the livers,colloid mass and melanomacrofages in the kidney, congestion and proliferation of secondary lamellae, limfosit and macrofag infiltration of the brain, a melanomacrofag of the muscle and the intestine showed there was limphocytes, and macrofages infiltration in the brain, and melanomacrofages in the muscles as well. It was suspected that those bacteria was  Edwardsiella tarda.   
IDENTIFIKASI CLINOSTOMUM COMPLANATUM SECARA MOLEKULER PADA IKAN AIR TAWAR DI YOGYAKARTA DAN RIAU (IDENTIFICATION OF CLINOSTOMUM COMPLANATUM FROM FRESHWATER FISH IN YOGYAKARTA AND RIAU BASED ON MOLECULAR STUDY) Riauwaty, Morina; ., Kurniasih; Prastowo, Joko; ., Windarti
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of study was, to identify Clinostomum complanatum (Digenea: Clinostomidae) infectingfreshwater fish in Yogyakarta and Riau on the bases of their molecular profiles in the internal transcribedspacer region (ITS1). Samples of climbing gouramy (Anabas testudineus) infected by Clinostomum sp. wereobtained from Kali Progo River, Yogyakarta. Whereas the climbing perch (Trichogaster trichopterus) wereobtained from the Sail River, Riau. Metacercariae of Clinostomum sp. found in the gills and visceralorgans were aseptically removed using needle, preserved in absolute ethanol. Molecular examination wasperformed by Polymerase Chain Reaction method consisted of extraction, amplification, electrophoresisand sequencing of DNA sample. The DNA sewuences of the samples were analysed by maximum parsimonyand neighbour-joining method. Phylogenetic analysis showed that Clinostomum sp. from Yogyakarta wasgenetically idential to Clinostomum complanatum, whereas Clinostomum sp. from Riau was geneticallysuspected as a new species (difference > 2%) which is included in one cluster to Clinostomum phalacrocorasis.
DAYA OVICIDAL EKSTRAK KULIT BUAH MUDA (CALOTROPIS PROCERA) TERHADAP HAEMONCHUS CONTORTUS SECARA IN VITRO Wirawan, I Gusti Komang Oka; Nurcahyo, Wisnu; Prastowo, Joko; ., Kurniasih
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1127.23 KB) | DOI: 10.22146/jsv.17891

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Penelitian ini bertujuan untuk mengetahui konsentrasi efektif ekstrak kulit buah muda Calotropis procera (C. procera) dalam menghambat perkembangan telur cacing Haemonchus contortus secara in-vitro. dari 0,2g/ml sediaan larutan ekstrak stok, albendazole konsentrasi 0,055%. Data hasil penelitian dianalisis secara deskriptif. Konsentrasi efektif ekstrak kulit buah muda C. procera (EKBMCP) dalam menghambat perkembangan daya tetas telur cacing Haemonchus contortus secarain-vitro adalah perlakuan EKBMCP konsentrasi 4,5% dengan daya hambat 88% sedangkan  perlakuan EKBMCP konsentrasi 2,5% dan 3,5% daya hambatnya secara berturut-turut adalah 70,5% dan 81%.
KEKERABATAN GENETIK CAPLAK RHIPHICEPHALUS (BOOPHILUS) MICROPLUS ASAL INDONESIABERDASARKAN SEKUEN INTERNAL TRANSCRIBED SPACER-2 (GENETIC RELATIONSHIP INDONESIAN RHIPHICEPHALUS (BOOPHILUS) MICROPLUS TICK BASED ON INTERNAL TRANSCRIBED SPACER-2 SEQUENSE ) Sahara, Ana; Prastowo, Joko; Widayanti, Rini; ., Kurniasih; Nurcahyo, Wisnu
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Rhiphicephalus (Boophilus) microplus is important obligatory blood feeding ectoparasites transmittingmany different viral, bacterial and protozoan and plays a role as a vector of Babesia sp., The leria sp. andAnaplasma sp. in cattle. The accuracy in identifying and distinguishing interspecies and intraspeciesdiversity among parasites is needed to understand the epidemiology, biology and capacity as a vector.Variations in the DNA base sequence of the internal transcribed spacer region2 (ITS 2) has been used asa molecular marker for identification in an effort to determine phylogenetic relationships. The aim of thisstudy was to determine the ITS 2 gene nucleotide sequence of R. microplus, which was expected to beuseful for accurate identification the parasite diversity and phylogenetic relationship among many differentspecies. DNA amplification was conducted using BOO2 forward dan BOO2 reverse primers. The DNAsamples containing ITS2 region fragment of 1099 nt were derived from the nucleotide sequence multiplealignments of R.microplus and other ticks genes obtained from Gene bank using Clustal W software, andthen analyzed using the MEGA program version 6. Genetic distances based on nucleotide sequence weredetermined with Kimura 2-parameter method producing the smallest genetic distance of 0 % and 1.2 %.Construction of phylogenetic trees using the Neighbor joining method showed that ticks from variousregions in Indonesia was species complex which have a closer with R.microplus isolates from India, Laos,South Africa, China and Australia R.australis origin.
DETEKSI BOVINE HERPESVIRUS-1 SECARA IMMUNOHISTOKIMIA PADA MEMBRAN KORIOALLANTOIS TELUR AYAM BEREMBRIO (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Kristianingrum, Yuli Purwandari; Tabbu, Charles Rangga; Sutrisno, Bambang; Widyarini, Sitarina; ., Kurniasih; Untari, Tri; Kusumawati, Asmarani
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.