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SEKUEN GEN SURFACE ANTIGEN-1 DAN BRADIZOIT ANTIGEN-1 TAKIZOIT TOXOPLASMA GONDII SEBAGAI KANDIDAT PEMINDAI DNA (SAG1 AND BAG1 GENE SEQUENCES ANALYSIS OF TOXOPLASMA GONDII TACHYZOITE AS PROBE CANDIDATE) Apsari, Ida Ayu Pasti; Artama, Wayan Tunas; ., Sumartono; Damriyasa, I Made
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Sag1 and bag1 is a gene specific-stage for Toxoplasma gondii tachyzoite and bradyzoite. The purposeof this study was analyze the sequences of sag1 and bag1 tachyzoite genes of local of Toxoplasma gondiiisolate as deoxyribonucleic acid probe candidate. Tachyzoite of local of Toxoplasma gondii isolate used onthis study. Gene sag1 and bag1 gene of Toxoplasma gondii were amplified by PCR, and then sequenced. Theresults showed sag1 fragment gene contained 612 bp and bag 1 contained 470 bp in length. BLAST analysisof sag1 and bag1 gene fragments as probe candidate showed that high specific for Toxoplasma gondii andno significant cross-reaction fragment with host and other parasites. The sequences 136 bp and 98 bpfragments as DNA probe candidate of Toxoplasma gondii sag1 and bag1 respectively.
KAJIAN SEMIOTIKA PADA INTERIOR GEREJA SANTO YAKOBUS SURABAYA Sutiono, Rezca Navtalia; ., Sumartono; Santosa, Adi
Dimensi Interior Vol 7, No 1 (2009): JUNI 2009
Publisher : Institute of Research and Community Outreach - Petra Christian University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.004 KB) | DOI: 10.9744/interior.7.1.pp. 40-51

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As can be seen from its ornamental elements, the interior of the Santo Yakobus Surabaya Church expressed meaningful signs. These signs serve as a representation of the Catholic liturgy with universal characteristics that become a reference for the Catholic church design in all over the world. This paper aims to reveal the meanings of these signs from the point of view of Peircean semiotics. In this case, these signs are analyzed through the categories of icon, index, and symbol respectively and then combined with the analyses of denotation, connotation and social aspect. The analyses result that the signs in this church convey meanings not only related to the Catholic liturgy but also local and social contexts.
CLONING OF THE GENE ENCODING SAGI OF LOCAL ISOLATE TOXOPLASMA GONDII IN ESCHERICHIA COLI DH5A Hartati, Sri; Widada, Sri; ., Sumartono; Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3103.953 KB) | DOI: 10.22146/jsv.503

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DEVELOPING FOREIGN LANGUAGE PROFICIENCY TROUGH SPEECH LEARNING IN EARLY CHILDHOOD ., Sumartono
CAKRAWALA Vol 4, No 7 (2010)
Publisher : CAKRAWALA

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Abstract. Childhood is generally regarded as the most cheerful phase in people life. Related to the language learning, in this period of time a child unconsciously learns how to speak his/her vernacular trough constant imitation and repetition of the utterances produced by the older people when they are speaking in their mother tongue language. This phenomenon takes place when the child is about six to eight years old. In the years after, a foreign language will be effectively taught, because they are supposed to be adult linguistically, which means that tha child is going to get ready to learn a new set of habits in speaking. Several characteristics of children as young learners of a foreign language should be considered as a guideline to decide several kinds of approach, methods, techniques, and models of foreign language teaching and learning to optimize their foreign language proficiency, considering that they have specific and unique characteristics contrasted to adult learners. To yield an intended result, appropriate, accurate and integrated materials involving pronounciation, vocabulary enrichment and sentence arrangement must be selected in such a way and adjusted to the learners’ needs. It’s not easy to achieve the intended purpose of the process due to several handicaps such as; lisping, slurring, stuttering, and cluttering. Key words : Childhood, vernacular, adult linguistically,foreign language proficiency, handicaps.
Analysis of Toxoplasma gondii Repeat Region 529 bp (NCBI Acc. No. AF146527) as a Probe Candidate for Molecular Diagnosis of Toxoplasmosis Pratama, Dyah Ayu Oktavianie A; ., Sumartono; Artama, Wayan T.
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

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            Toxoplasmosis is a disease caused by protozoan parasite Toxoplasma gondii. The infection is commonly asymptomatic. The availability of confirmative and accurate detection system is really needed. This research was aimed to develop a molecular diagnosis based on the conserved and high copy number repeat region of Toxoplasma gondii with hibridization method. Nucleic acid was isolated from tachyzoites. The repeat region of T. gondii was amplified using PuRe Taq Ready To Go-PCR Beads (Amersham Bioscience),  forward primer 5’- GAC TCG GGC CCA GCT GCG  -3’ and reverse primer 5’- CCT CTC CTA CCC CTC CTC -3’. The amplicon was sequenced using ABI Prism 3100-Avant Genetic Analyzer (PT. Charoen Pokphand, Jakarta). Probe was labeled using digoxigenin-11-dUTP. Application of probe to detect it’s complementary nucloeic acid was done by hibridization method. The research concluded that probe toxo-103 bp was highly homolog with several strain of T. gondii and it has no homology either with host’s genome or other parasites which have close genetic relationship with T. gondii.   Hybridization analysis showed that probe could detect the complementary nucleic acid up to 10 ng/ul concentration.      
KLONING DAN EKSPRESI GEN PENYANDI SAGI TOXOPLASMA GONDII ISOLAT LOKAL PADA VEKTOR PGEX-2T Hartati, Sri; Wuryastuti, Hastari; Widada, Joannes Sri; ., Sumartono; Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1167.607 KB) | DOI: 10.22146/jsv.453

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Towards Communication Channels That Enlighten ., Sumartono
Communication Sphere Vol 1, No 1 (2015): November
Publisher : Communication Sphere

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ABSTRACTStudy on the channel of political communication (media) boils down to two thingsnamely, accuracy of channels selection, and utilization of existing channels. This understandingis important and affect the success of political activity that will be executed. Usually the failure ofthe political influence the choice of a constituent is not only caused by the Communicator ormessages to be delivered but also on political communication or media channels. In otherwords, political communication channel component is as important as the existence of a politicalCommunicator. This means that errors in choosing political influential communication channelssignificantly to selection or selectivity of society.Keywords: Channels, Political Communications, Enlighten
IMPLEMENTASI KEBIJAKAN PENANGGULANGAN KEMISKINAN MELALUI KURMIKRO PT. BRI UNIT SOEKARNO-HATTA KOTA MALANG Dharma, Buyung Adi; ., Sumartono; ., Sarwono
Jurnal Ilmiah Ilmu Administrasi Publik Vol 6, No 1 (2016)
Publisher : Program Pascasarjana Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26858/jiap.v6i1.2071

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Latar belakang penelitian ini adalah masih adanya permasalahan kemiskinan di Indonesia membutuhkan penanganan dari pemerintah untuk segera ditanggulangi. Perpres No. 15 Tahun 2010 tentang percepatan penanggulangan kemiskinan menjadi kebijakan yang secara khusus mengatur tentang percepatan penanggulangan kemiskinan, dimana diantaranya memuat tentang kebijakan pemberian Kredit Usaha Rakyat bagi pelaku usaha di Indonesia. Penelitian ini sebagai kajian khusus terhadap PT. BRI Unit Soekarno-Hatta kota Malang. Metode penelitian yang digunakan adalah metode kualitatif dengan pendekatan deskriptif. Hasil penelitian ini menunjukkan bahwa kinerja PT. BRI Unit Soekarno - Hatta dalam implementasi kebijakan Kredit Usaha Rakyat Mikro ditinjau dari aspek isi dan lingkungan dari kebijakan menunjukkan hasil yang sangat baik. Faktor yang mendukung adalah karakteristik PT. BRI Persero Tbk yang merupakan profesional dibidang perbankan. Permasalahannya adalah keterbatasan PT. BRI Persero Tbk dalam melakukan kegiatan monitoring dan evaluasi atas pengentasan kemiskinan sesuai dengan tujuan dari Peraturan Presiden No. 15 Tahun 2010. Kebijakan KUR Mikro membawa dampak yang positif bagi laju ekonomi dan usaha UMKM. PT. BRI dalam penyaluran KUR Mikro dengan target yang tinggi akan meningkatkan resiko terjadinya kerugian akibat munculnya kredit macet sehingga untuk menghindarinya maka permohonan kredit harus dinilai oleh sesuai profesionalisme lembaga perbankan. Kata Kunci:   Implementasi kebijakan, Penanggulangan kemiskinan, Kredit Usaha rakyat.
STUDI EKSPERIMENTAL SISTA JARINGAN TOXOPLASMA GONDII SECARA IN VIVO AN EXPERIMENTAL STUDY OF TOXOPLASMA GONDII TISSUE CYST IN VIVO ., M.Hanafiah; Nurcahyo, Wisnu; ., Sumartono
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2999.015 KB) | DOI: 10.22146/jsv.498

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ANALISIS HOMOLOGI SEKUEN FRAGMEN GENOM EIMERIA TENELLA ISOLAT JEPANG, JOGJA DAN SOLO ., Sumartono
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1599.446 KB) | DOI: 10.22146/jsv.490

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