Supramana .
Institut Pertanian Bogor

Published : 4 Documents

Found 4 Documents

Identifikasi Spesies Meloidogyne spp. Penyebab Umbi Bercabang pada Tanaman Wortel di Jawa Timur Hikmia, Zalzilatul Hikmia; ., Supramana; Suastika, Gede
Jurnal Fitopatologi Indonesia Vol 8 No 3 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1002.756 KB) | DOI: 10.14692/jfi.8.3.73


Branched tuber is a new disease in carrot cultivation in Indonesia and has caused big losses for farmers. In 2010, several species of root-knot nematodes, Meloidogyne spp., were reported as the primary cause of disease and the yield loss due to the disease in the region of Agropolitan-Cianjur, West Java, reached 15% to 95%. Symptoms of tuber branching has also been reported in one of the centers of vegetable production in East Java, in the subdistrict of Bumiaji, Kota Batu. Research aimed at identifying  Meloidogyne species on carrot was carried out on carrot plantation. The study consisted of two activities, i.e. surveillance and identification. Surveillance was carried out at three different altitudes/elevations, i.e. 1600 m, 1700 m and 1800 m asl (above sea level). Identification was done by PCR ITS r-DNA using multiplex primer for mix spesies identification of M. hapla, M. fallax, and M. chitwoodi and the spesific primer for identification of M. arenaria, M. incognita, and M. javanica. Identification based on ITS r-NA by PCR gave positive results for four species, namely M. hapla, M. arenaria, M. incognita, and M. javanica.Key words: branched tuber disease, carrot, Meloidogyne, PCR ITS r-DNA
Identifikasi Meloidogyne Penyebab Penyakit Umbi Bercabang pada Wortel di Dataran Tinggi Dieng Taher, Muhamad; ., Supramana; Suastika, Gede
Jurnal Fitopatologi Indonesia Vol 8 No 1 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.899 KB) | DOI: 10.14692/jfi.8.1.16


Branched tuber disease is a new problem on carrot cultivation in Indonesia. The disease may reduce the quantity and quality of the tubers and causing significant yield losses. Meloidogyne spp. has been identified as the primary cause of branched or forked disease of carrot in West Java. Similar disease was observed in carrot field in Dieng Plateau, Central Java. The study was conducted at three locations in the Dieng Plateau having different elevation. Nematode species identification was done by observing the perinneal pattern of 150 samples of female nematode and obtained four Meloidogyne species, namely M. arenaria, M. hapla, M. incognita, and M. javanica. All four species were found associated with branched tuber disease in three locations with M. incognita as a dominant species having prevalence levels of 50% to 58%.Key words: branched tuber disease, carrots, Meloidogyne, species identification
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.681 KB) | DOI: 10.23960/j.hptt.21699-106


Effectivenes of Brassicaceae plant wastes to control the root knot nematodes (Meloidogyne spp.) at a field microplot scale. Meloidogyne spp. is a soil borne pathogen that infects plant roots and causes root galls. Root knot nematodes can reduce crop production by 15 to 95%, so that the control measures are needed. One of the control methods is using plants as biofumigant. Plants of the family Brassicaceae were reported contain glucosinolate (GSL). During decomposition, GSL is hydrolized to isothiocyanates (ITS) which is a highly toxic compound to soil organisms, including nematodes. The research objective was to determine the effectiveness of five Brassicaceous plant wastes, namely cabbage (B. oleracea var capitata), radish (Raphanus sativus), broccoli (B. oleracea var italica), chinese cabbage (B. chinensis) and pakcoy (B. rapa var parachinensis) to suppress root knot nematodes (RKN). The experiment was conducted on microplot scale in the field. The experimental design used was a 4x5 factorial CRD. The first factors are waste of Brassica and the second factors are the amount of Brassica wastes per microplot. The experiments were made in 6 replications. Application of 5 Brassica plant wastes at all doses tested effectively reduced the number of root knot by 45.65% to 94.43% and increased the average number of tomato fruits. Tomato plants grew better at microplots when treated with chinese cabbage and pakcoy wastes.
Pathogenicity and Effectiveness of Entomopathogen Nematode Heterorhabditis sp. to Potato Tuber Moth Phthorimaea operculella (Zeller)(Lepidoptera: Gelechiidae) PATOGENISITAS DAN KEEFEKTIFAN NEMATODA ENTOMOPATOGEN Heterorhabditis sp. TERHADAP PENGGEREK UMBI Rusniarsyah, Lufthi; Rauf, Aunu; ., Supramana; ., Samsudin
Jurnal Silvikultur Tropika Vol. 6 No. 1 (2015): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.183 KB)


Potato tuber moth, Phthorimaea operculella Zell. (Lepidoptera: Gelechiidae) is one of the important potato pest. Damage occurs in the field as well as in the storage warehouses. If control is not performed well, the potential loss will reach almost 100%. The effectiveness of entomopathogenic nematodes of the Heterorhabditis has been studied to control several crop pests. Infective juveniles (IJ’s) of nematodes are capable to seek and infect insects that live in soil and in plant tissues. The objectives of the research are to study the pathogenicity of the Heterorhabditis sp. against P. operculella in the laboratory in direct treatment and in the potato tuber. The experiment was employed a completely randomized design with 6 treatments and 4 replications. The treatment tested are the density level of nematodes Heterorhabditis sp. (100, 200, 300, 400, and 500 IJ/ml) and the control. The results showed that P. operculella infected by Heterorhabditis sp. has symptoms of decreasing motion and feeding activity. Dead insects have change color to dark brown and the body become soft. Heterorhabditis sp. can kill P. operculella within 12 hours after application (HAA). At density level 100 IJ/ml it can control P. operculella by 85% within 24 HAA. The mortality rates of P. operculella in the tubers was 35% at doses of 1000 IJ/tubers, although the ANOVA test was not significantly different.Key words: Phthorimaea operculella, entomopathogenic nematodes, Heterorhabditis sp.