Mahdi Abrar
Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh

Published : 42 Documents
Articles

DETEKSI ANTIBODI SERUM TERHADAP VIRUS AVIAN INFLUENZA PADA AYAM BURAS Darmawi, Darmawi; Manaf, Zakiyah Heryawati; Darniati, Darniati; Fakhrurrazi, Fakhrurrazi; Abrar, Mahdi; Erina, Erina
Jurnal Agripet Vol 12, No 1 (2012): Volume 12, No. 1, April 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v12i1.283

Abstract

Detection on Serum Antibodies of Native Chickens to Avian influenza VirusABSTRACT.  An important approach of controlling against Avian Influenza should be determined to detect the antibody titres of bird flu caused by Influenza virus H5N1 in Indonesia. The aim of the present study was to detect the antibodies to Avian Influenza in serum of native chickens. This study utilized 123 serum samples collected from the axilaris vein (left or right) of native chickens. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that indication of natural infection by Avian Influenza (H5N1) in native chickens, as shown that out of 123 serum samples, 16 (13,01%) were tested positive by HI, while only 10 (8,13%) were tested protective to Avian influenza infection. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus stimulated variety level of formation antibody titres in native chickens.
ISOLASI DAN KARAKTERISASI HEMAGLUTININ STAPHYLOCOCCUS AUREUS PENYEBAB MASTITIS SUBKLINIS PADA SAPI PERAH Abrar, Mahdi; Wibawan, I Wayan Teguh; Priosoeryanto, Bambang Pontjo; Soedarwanto, Mirnawati; Pasaribu, Fachriyan Hasymi
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i1.347

Abstract

Dalam penelitian ini isolasi dan karakterisasi hemaglutinin Staphylococcus aureus dilakukan dengan teknik afinitas kromatograf Karakterisasi hemaglutinin yang dihasilkan dilajutkan dengan teknik elektroforesis menggunakan metode sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) dan dilanjutkan untuk melihat pengaruh suhu dan enzim terhadap aktivitas hemaglutinin. Hasil penelitia menunjukkan bahwa komponen hemaglutinin Staphylococcus aureus yang telah diisolasi memiliki berat molekul 46 kDa. Aktivit Staphylococcus aureus dalam meghemaglutinasi hilang pada pemanasan 60° C dan pengaruh enzim proteolitik. Hasil ini mengindikasika bahwa hemaglutinin Staphylococcus aureus adalah protein.
PERANAN HEMAGLUTININ STAPHYLOCOCCUS AUREUS DALAM PROSES ADHESI PADA SEL EPITEL AMBING SAPI PERAH Abrar, Mahdi; Wibawan, I Wayan Teguh; Priosoeryanto, Bambang Pontjo; Soedarwanto, Mirnawati; Pasaribu, Fachriyan Hasymi
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.564

Abstract

Tujuan penelitian ini adalah mengetahui kemampuan hemaglutinin pada Staphylococcus aureus (S. aureus) dalam proses adhesi. Uji adhesi pada sel epitel ambing dilakukan dengan menggunakan metode Velentine-Weiggand. Hasil penelitian ini menunjukkan bahwa S. aureus yang memiliki hemaglutinin menunjukkan kemampuan adhesi yang jauh lebih baik (±380 bakteri/20 sel) dibandingkan dengan yang tidak memiliki hemaglutinin (±56 bakteri/20 sel).
ISOLATION OF ENTEROBACTERIACEAE BACTERIA SPECIES FROM FECES OF SUMATRAN ORANGUTANS (PONGO ABELII) Darmawi, .; Rahmi, Erdiansyah; Dewi, Maryulia; Hutabarat, Joharsyah; Abrar, Mahdi; Fakhrurrazi, .
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The porpouse of this research aimed to isolate enterobacteriaceae bacteria from feces of Pongo abelii. The samples of feces were collected from 15 captive orangutans in Orangutan Sumatera Batu Mbelin Sibolangit Qurantine, North Sumatra. Of each sample was cultured in nutrient broth media using sterilecotton swabs or Pasteur pipettes, and incubated at 37°C temperature for 24 hours. Culture was spared on Methylene Blue Agar (Oxoid), examined by Gram staining, and tested by biochemically. The result showed that significantly more common appear Escherichia sp. (93,33%) and fewer Edwardsiella sp. (66,67%) wereisolated from feces samples of P. abelii. Others enterobacteriaceae found in feces of P. Abelii were Shigella sp. (46,67%), Klebsiella sp. (33,33%), Citrobacter sp., and Salmonella sp. (13,33%), respectively
ACEH CATTLE FOLLICLE DYNAMIC UNDER ENVIRONMENTAL HEAT STRESS Armansyah, Teuku; Siregar, Tongku Nizwan; Aliza, Dwinna; Melia, Juli; Meutia, Nellita; Panjaitan, Budianto; Hafizuddin, Hafizuddin; Adam, Mulyadi; Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.6377

Abstract

The objective of this research is to know Aceh cattle follicle dynamic under environmental heat stress condition. This research was conducted on December 2012 until August 2013. Animals used in this research were 20 Aceh cattle aged 5-8 years old, weighing of 150-250 kg, and had at least 2 regular cycles. All cattle used were clinically in good body condition score. The cattle were divided into two groups of 10, used in two different period of times (December-January and July-August) to examine the effect of environmental heat to their follicle dynamic. Each group is separated into two different keeping management; one group was kept in pens while the others in the pasture, 5 cattle in each management group. Research timing was based on information acquired from Indrapuri Agency for Meteorology, Climatology, and Geophysics (BMKG) which predicted that extreme weather would last from July to August while December to January would be relatively normal in Aceh region. All cattle?s estrous cycle were synchronized by 5 mg/mL of PGF2? intramuscularly (Lutalyse?, Pharmacia Upjohn Company, Pfizer Inc.). Ultrasonography examination was performed to monitor ovary follicle?s growth and dynamic during one cycle. Days during ovulation marked by standing heat was regarded as Day 0 of estrous cycle. Follicular dynamic examination during estrous cycle on December-January and also on July-August, both penned or pastured cattle showed the follicle waves that was 3 follicle growth waves. The size of follicle growth on first wave (1st DF) on cattle kept in pen on July-August and December-January were relatively similar. The size of follicle growth on first wave for cattle kept in pasture on July-August and December-January were relatively similar. To conclude, Aceh cattle?s follicle dynamic is not change during environment heat stress condition and is not affected by different cattle-keeping management.
INVASIF DAN PERSISTEN PASTEURELLA MULTOCIDA SETELAH DIINOKULASI SECARA INTRATRACHEAL PADA KALKUN Abrar, Mahdi; Fakhrurrazi, Fakhrurrazi
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9815

Abstract

The objective of this study was to evaluate the invasiveness and persistence of Pasteurella multocida after intratracheal inoculation on turkeys. Four strains of Pasteurella multocida, P-1059, T-325, CU, and M-9 were used in this study and intratracheally (IT) inoculated into turkeys. The number of viable bacteria in the trachea, lung, liver, spleen, and the blood were enumerated during periods of 6 and 9 hrs post inoculation (PI). The result showed that four strains of organism were present in the organ and the tissues observed. In the liver, spleen, and blood, the virulent P-1059 strain showed significant increases within 6 or 9 hrs PI. Strain CU showed significant increase only in the spleen. Two other strains did not show significant increase in any organs; strain M-9, on the other hand, showed significant decrease in the trachea. These results indicated that P-1059 and three other strains showed their ability to invade the tissues or organs when they are intratracheally inoculated.
PERANAN HEMAGLUTININ ESCHERICHIA COLI DALAM PROSES ADHESI Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 3, No 1 (2009): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i1.3076

Abstract

Adhesi merupakan tahap inisiasi dari proses kolonisasi bakteri. Kemampuan adhesi ini diperantarai oleh keberadaan hemaglutinin pada permukaan bakteri. Tujuan penelitian ini adalah melihat kemampuan hemaglutinin pada Escherichia coli dalam proses adhesi. Uji adhesi pada sel epitel pipi dilakukan dengan menggunakan metode Velentine-Weiggand. Hasil penelitian menunjukkan bahwa Escherichia coli yang memiliki hemaglutinin menunjukkan kemampuan adhesi yang jauh lebih baik yakni rata-rata 67,6 bakteri/sel dibandingkan dengan   yang tidak memiliki hemaglutinin rata-rata 7,2 bakteri/sel. Kemampuan adhesi ini berkaitan dengan keberadaan hemaglutinin di permukaan bakteri.
CORRELATION BETWEEN PROGESTERONE HORMONE CONCENTRATION AND NUMBER OF FETUSES OF ETAWA CROSSBRED GOAT IN DIFFERENT GESTATION PERIOD Syafruddin, Syafruddin; Rizal, Khairul; Rosmaidar, Rosmaidar; Melia, Juli; roslizawaty, roslizawaty; Zuhrawati, Zuhrawati; Rahmi, Erdiansyah; Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.8687

Abstract

This study aimed to investigate the correlation between progesterone hormone concentration and number of fetus as well as the difference in progesterone hormone concentration from different gestation periods in Etawah crossbred (EC). Five EC goats used in this study were injected with 2 mL of 5.5 mg prostaglandin F2? (PGF2?, Capriglandin) intramuscularly, with an interval of 10 days. Goats that showed symptoms of estrus were mated naturally with male goats. Blood samples were taken on the 7th, 14th, 21st, and 75th days of gestation. Progesterone hormone concentration was determined using enzyme linked immunosorbent assay (ELISA). The number of fetus was determined at 35th gestational day using ultrasonography (USG). Four out of 5 goats were found to have 1 fetus and 1 goat had 2 fetuses. The progesterone concentration on the 7th, 14th, 21st, and 75th days of gestation in goats with single fetus were 5.21, 7.78, 11.97, and 18.78 ng/mL, respectively, while in goat with two fetuses were 8.44, 14.53, 16.81, and 22.73 ng/mL, respectively. The correlation (r) between progesterone hormone concentration and number of fetus on the 7th, 14th, 21st, and 75th days of gestation were 0.442, 0.854, 0.592, and 0.757, respectively. It is concluded that progesterone concentrations are highly correlated to the number of fetus in each gestation period in EC goats.
SEROPREVALENSI AVIAN INFLUENZA H5N1 PADA UNGGAS DI KABUPATEN ACEH UTARA Darmawi, Darmawi; Darniati, Darniati; Dewi, Maryulia; Fakhrurrazi, Fakhrurrazi; Abrar, Mahdi; Erina, Erina
Jurnal Agripet Vol 13, No 2 (2013): Volume 13, No. 2, Oktober 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v13i2.815

Abstract

Seroprevalence of avian influenza H5N1 in birds in north aceh districtABSTRACT. Avian influenza virus H5N1 infections are an important cause of diseases in humans and several animal species, including birds. The present study conducted to investigate the seroprevalence Avian Influenza H5N1 in native birds from 15 sub-districts of North Aceh.  This study utilized 1108 serum samples collected from the axilaris vein (left or right) of birds. The standard Hemaglutination Inhibition (HI) assay was conducted at Microbiology Laboratory Faculty of Veterinary Medicine of Syiah Kuala University to determined serum antibody possitive or negative reaction against Avian influenza H5N1. The result showed that seroprevalence Avian influenza H5N1 virus was 4,7 % in North Aceh District. There were nine sub-districts were tested positively by HI test. However, the serum collected from six sub-districts did not react (negative) against Avian influenza H5N1. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus in native birds occured in part of North Aceh District.
ISOLASI BAKTERI Pseudomonas sp PADA IKAN ASIN TALANG- TALANG (Scomberoides tala) DIDESAPULOET KECAMATAN LEUPUNGKABUPATEN ACEH BESAR Arvina, Arvina; Fakhrurrazi, Fakhrurrazi; Abrar, Mahdi
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.49 KB) | DOI: 10.21157/jim vet..v1i3.3939

Abstract

Penelitianini bertujuan mengisolasi bakteri Pseudomonas sp pada 7 ekor ikan asin Talang-Talang di Desa Puloet Kecamatan Leupung Kabupaten Aceh Besar. Isolasi bakteri dilakukan dengan cara menghaluskan daging ikan menggunakan blander steril, selanjutnyadipupuk pada media Nutrient Brooth (NB), dihomogenkan dan diinkubasi pada temperatur 370C selama 24 jam, setelah itu digoreskan pada media Tryticase Soya Agar (TSA). Hasilpenelitian tidak ditemukan pertumbuhan koloni bakteri Pseudomonas sp pada media TSA, dengan demikian dapat disimpulkan bahwa tidak ada bakteri Pseudomonas sppada sampel ikan asin Talang-Talang di Desa Puloet Kecamatan Leupung Kabupaten Aceh Besar.Penelitianini bertujuan mengisolasi bakteri Pseudomonas sp pada 7 ekor ikan asin Talang-Talang di Desa Puloet Kecamatan Leupung Kabupaten Aceh Besar. Isolasi bakteri dilakukan dengan cara menghaluskan daging ikan menggunakan blander steril, selanjutnyadipupuk pada media Nutrient Brooth (NB), dihomogenkan dan diinkubasi pada temperatur 370C selama 24 jam, setelah itu digoreskan pada media Tryticase Soya Agar (TSA). Hasilpenelitian tidak ditemukan pertumbuhan koloni bakteri Pseudomonas sp pada media TSA, dengan demikian dapat disimpulkan bahwa tidak ada bakteri Pseudomonas sppada sampel ikan asin Talang-Talang di Desa Puloet Kecamatan Leupung Kabupaten Aceh Besar. Kata kunci : Ikan asin Talang-Talang, Pseudomonas sp, di Desa Puloet Kecamatan Leupung Kabupaten Aceh Besar. ABSTRACT                     The aim of this study is to isolate Pseudomonas sp bactery on 7 Talang-Talang salted fish at Puloet village Leupung District Aceh Besar Regency. The bacterial isolation was done by blending the sample using the sample that had blender  then cultivated  in  Nutrient Brooth (NB), homogenated then incubated at temperature 370C for 24 hours, Then each sampel was fertilized on Tryticase Soya Agar (TSA) media and incubated at temperatur 370C for 24 hours. The result of this research shows that no Pseudomonas sp bacteria on Talang-Talang salted  fist at Puloet Village LeupungDistrict Aceh Besar Regency. Keywords: Talang-Talang salt fish, Pseudomonas sp, at Puloet village District Leupung Regency Great Aceh.
Co-Authors . Darmawi . Fakhrurrazi Admi, Masda Afriyani, Afriyani aini, hafitatul AK, M. Daud Amri, M. Chairul Arman Sayuti Arvina, Arvina Athaillah, Farida BAMBANG PONTJO PRIOSOERYANTO budianto panjaitan Darmawi -, Darmawi Darmawi Darmawi Darniati Darniati Daud AK, M Dwinna Aliza Erdiansyah Rahmi Erina Erina Erni Wulandari, Erni Fachriyan Hasymi Pasaribu Faisal Jamin Fakhrur Razi Fakhrurrazi - Fakhrurrazi Fakhrurrazi Ferasyi, T.Reza Gholib Gholib, Gholib Girsang, Florentina Magdalena Hafizuddin Hafizuddin, Hafizuddin Hamny Hamny Harahap, Abdullah Azmi Helmi, T. Zahrial Herrialfian, Herrialfian Hutasoit, Kabetty I WAYAN TEGUH WIBAWAN Ismail Ismail Joharsyah Hutabarat Joni, Liza Setia Juli Melia Juliani Juliani Khairiah Khairiah M. Nur Salim Maryulia Dewi maulita, riza Melawati, Bahagia Mirnawati Soedarwanto Mulyadi Adam, Mulyadi Muslim Akmal Muttaqien Bakri, Muttaqien Nanda Yuliansyah, Nanda Nellita Meutia, Nellita Nurliana N, Nurliana NURLIANA NURLIANA Nuryani, Srirezeki Handa Nuzul Asmilia Pratama Sekedang, M. Iqbal RAHMADIAN, CUT ADE Ramadhani, Devi Rastina, Rastina Razali Daud Razali Razali Rinidar Rinidar, Rinidar Rizal, Khairul Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar, Rosmaidar Salim, Muhammad Nur Sari, Nelma Siregar, Chairul Saputra Sitakar, Nurdiani Muliana Sri Wahyuni Sugito s Sugito Sugito Sulasmi Sulasmi Suyoto, Balqis Alrasyid Syafruddin - Syafruddin Syafruddin T. Armansyah T. Fadrial Karmil Teuku Armansyah Teuku Zahrial Helmi Tongku Nizwan Siregar udin, mukthar Ulfah, Izdaharra Mutia Vhodzan Adzima, Vhodzan Wiliana -, Wiliana Winaruddin Winaruddin Yanrivina S.V.S, Yanrivina Yudha Fahrimal Yulina Rahmi, Yulina Zahra, Amalia Fatma Zakiah Heryawati Manaf Zakiah Heryawati Manaf, Zakiah Zakiah Heryawaty Manaf, Zakiah Heryawaty Zakiyah Heryawati Manaf Zuhrawati Zuhrawati, Zuhrawati Zulfan, Muhammad