Marlina Achmad
Jurusan Perikanan, Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin, Makassar, Sulawesi Selatan

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Karakteristik Jaringan Secara Histologi dari Strain Rumput Laut (Kappaphycus alvarezii) yang Terinfeksi Penyakit Ice-Ice Maulani, Resi Kumala; Achmad, Marlina; Latama, Gunarto
TORANI: Journal of Fisheries and Marine Science VOLUME 1 NOMOR 1, DESEMBER 2017
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v1i1.3796

Abstract

 Salah satu yang menyebabkan kegagalan budidaya rumput laut Kappaphycus alvarezii  yaitu terserangnya penyakitice – ice . Penyakit ice-ice merupakan penyakit yang umum terjadi pada rumput laut kappaphycus  dengan gejalaterjadinya pemutihan pada bagian thallus  dan penyakit ini dapat menyebabkan kematian massal pada produksirumput laut kappaphycus alvarezii . Salah satu cara yang dapat dilakukan untuk menganalisa bagian thallus  rumputlaut Kappaphycus alvarezii  yaitu uji histopatologi. Penelitian ini bertujuan untuk mengetahui perubahan bentuksel pada kondisi jaringan thallus  rumput laut K.alvarezii  yang terserang penyakit ice- ice  menggunakan metodehistopatologi. Pengambilan sampel rumput laut yang digunakan jenis Kappaphycus alvarezii  varietas coklat danhijau dengan 1 titik masing – masing varietas. Sampel rumput laut terdiri atas 2 sampel yaitu bagian thallus  yangterserang penyakit ice-ice  dan bagian thallus  tanpa gejala ice-ice . Sampel berumur 15 hari. Sampel yang dipilihuntuk pemeriksaan, dipotong setebal 0,5 - 1 cm. Sampel dimasukkan kedalam botol sampel untuk difiksasi ± 1minggu, yang berisi formalin 10%.Tahapan pembuatan preparat histopatologi terdiri atas fiksaksi, dehidrasi,clearing , impregnasi , embedding , cutting , staining , dan pemeriksaan dibawah mikroskop. Penelitian ini dianalisissecara dekskriptif kualitatif dengan melakukan pengamatan gambar histopatologi pada jaringan rumput laut K.alvarezii . Jaringan thallus  yang yang terserang penyakit ice-ice  mengalami perubahan bentuk sel, bagian lapisanluar terlihat tidak utuh, hancur, sehingga mengalami kematian sel dan bagian korteks dalam dinding sel terlihattidak teratur dan renggang, tidak terbentuk bulat atau lonjong, sebagian sel mengecil. Jaringan thallus  yang tidaknormal mengalami gejala Hipoplastik (terhambatnya atau terhentinya pertumbuhan sel) dan Nekrotik (kematiansel).Kata kunci : Kappaphycus alvarezii, penyakit ice – ice, Histopatologi, Hipoplastik, Nekrotik
Use of Probiotics to Fight Bacterial Populations of Vibrio sp. on Vaname Shrimp Cultivation (Litopenaeus vannamei) Mustafa, Muhammad Fadhil; Bunga, Margaretha; Achmad, Marlina
TORANI: Journal of Fisheries and Marine Science VOLUME 2 NOMOR 2, JUNI 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v2i2.7056

Abstract

The consequence of super intensive shrimp farming is an increase in aquaculture waste and disease transmission. Vibrio sp. Bacteria is a disease-causing agent in shrimp larvae when the shrimp is in a state of stress and weakness, and can cause death. The application of probiotics, namely beneficial microorganisms can degrade organic matter, reduce disease, and help accelerate the process of nutrient cycles as a consequence of super intensive shrimp farming. Commercial probiotics currently available are a mixture of several types of beneficial microorganisms, namely Bacillus sp., Pseudomonas sp., Nitrosomonas sp., Aerobacter sp., and Nitrobacter sp. The purpose of this study was to evaluate the use of probiotics with different time intervals to produce a decrease in the population of Vibrio sp. The research method applied was the treatment of commercial probiotics at different intervals of three, five, and seven days and without the administration of probiotics as controls. Furthermore, the bacterial population was calculated by total plate count (TPC). The results showed that the administration of probiotics had a significant effect on the decrease in the population of Vibrio sp. Provision of probiotics three days apart showed a decrease in the number of bacterial populations that were significantly different from the controls, but the same as the administration of intervals of five and seven days. The decrease in the number of bacterial populations in the treatment interval of three, five, and seven days in a row is 0.05 x , 0.41 x , and 0.61 x  CFU / ml. This study recommends that the use of commercial probiotics intervals of seven days for super intensive vaname shrimp farming. The use of probiotics for three days indicates an impact on the increase in expenditure costs for probiotics. Commercial probiotic studies modified with natural microorganisms are thought to be able to streamline the operational costs of shrimp farming.Keywords: Vibrio sp. bacteria, probiotics, vaname shrimp, super intensive cultivation
Identifikasi Ektoparasit Pada Ikan Koi (Cyprinus carpio) Di Makassar Bunga, Margaretha; Achmad, Marlina
TORANI: Journal of Fisheries and Marine Science VOLUME 1 NOMOR 1, DESEMBER 2017
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v1i1.3794

Abstract

 Penelitian bertujuan untuk mengidentifikasi ektoparasit pada ikan mas koi (Cyrinus carpio) di Makassar. Penelitiandilakukan dengan pengambilan sampel sebanyak tiga kali di bulan April hingga Mei 2017. Sampel ikandimasukkan ke dalam kantong plastik yang telah diisi dengan oksigen. Jumlah sampel adalah 45 ikan denganpanjang 5,5-11,8 cm dan berat 5,12 – 13,2 g. Identifikasi parasit dan penghitungan jumlah parasit dilakukan diLaboratorium Parasit dan Penyakit Ikan, Departemen Perikanan, Fakultas Ilmu Kelautan dan PerikananUniversitas Hasanuddin. Data dianlaisis secara deskriptif. Serangan ektoparasit menyerang ikan koi di bagianinsang yakni 34,21%, sirip dorsal 34,21%, dan pada mukus mengandung 31,58%.Kata kunci : ektoparasit, identifikasi, ikan koi
Penggunaan Gen GH sebagai Marka Molekuler DNA Gurami, Osphronemus goramy dalam Pengembangan Teknologi Surrogate Broodstock Achmad, Marlina; Alimuddin, Alimuddin; Carman, Odang; Arfah, Harton; Zairin, Muhammad
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.7

Abstract

The technology of fish germ cell transplantation had been established to create broodstock systems by which a target offspring can be produced from a surrogate parent. Donor cell for transplantation is derived from transgenic fish carrying green fluorescent protein gene functions as a marker to distinguish the donor from recipient cell. In this study, an alternative technique was developed for identifying gouramy-derived donor cell and Nile tilapia as recipient by PCR amplification method using growth hormone (GH) gene as a molecular marker.  Specific primer for GH gouramy was designed by using Genetyx version 7 software.  ß-actin gene was used as an internal control of DNA loading. The result showed that a specific PCR amplification product of 340 in length was obtained when the DNA template was from gouramy, while no PCR product from Nile tilapia. The minimum concentration of genomic DNA of gouramy mixed with a 700 ng/µl of Nile tilapia that could be detected by PCR was 1 ng/µl. Thus, PCR method with specific GH primer may be useful to detect the incorporation of donor cell in recipient gonad towards development of surrogate broodstock technology.