Anak Agung Ayu Mirah Adi
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HISTOPATOLOGYCAL BRONKIOLUS AND BLOOD VESSEL OF MICE LUNG POST EXPOSURE OF ELECTRIC CIGARETTE SMOKE Monica, Mia; Adi, Anak Agung Ayu Mirah; Winaya, Ida Bagus Oka
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.988 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p08

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This study aims to determine the histopathological changes of bronchioles and blood vessel of mice (Mus musculus) lung post exposed of electric cigarette smoke (vaping). This study was used 24 mice that were divided into two treatment groups. Group I (P0) as a control without electronic cigarette smoke exposured and group II (P1) consistied of 12 mice as a treatment group, were exprosure with electric cigarette smoke. This study used a complete randomized design (RAL) split in time pattern with three 1st, 2nd and 3rd post-treatment sampling times. Four mice of each group were taken at each observation time. Lung tissue was taken to make preparations and stained with Hematoxylin-eosin (HE) staining. The results showed that there were significant differences (P <0.05) between the treatment group and the control group, but the duration of exposure did not have an effect. Mucosal thickness in the treatment group was higher than in the control group (P <0.05). Vessel lung endothelins and thrombosis. It can be concluded, exposure to electric cigarette smoke can cause degeneration, cell necrosis and increase the mucosal thickness of the bronchioles. The vascular system experiences endotheliosis, endothelial cell necrosis and thrombosis
GAMBARAN HISTOPATOLOGI HATI DAN GINJAL ANJING TERDUGA LEPTOSPIROSIS DI KOTA DENPASAR (HISTOPATHOLOGICAL CHANGES OF LIVER AND KIDNEY DOG SUSPECTED CANINE LEPTOSPIROSIS IN DENPASAR CITY) Winaya, Ida Bagus Oka; Berata, I Ketut; Kardena, I Made; Adi, Anak Agung Ayu Mirah; Rompis, Aida Louise Tenden
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.475 KB) | DOI: 10.19087/jveteriner.2018.19.2.298

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This study aims to determine the pathological changes in dog suspected of being infected by Leptospira spp. This research used a retrospective study design.  A total of 210 canines sample were examined at Faculty of Veterinary Medicine The Udayana of University between January 2008 to January 2010. Of the 210 specimens, five canines with clinical sign anorexia, fever, vomiting, polyurea, dyspnea and only one are noted icteric in the sclera. Macroscopically : the anemic to petechial haemorrhagis  was find in pulmo, icterus in liver with gall blader distention, kidney swollen with black color in hillus, spleen rather swollen with black color on end and mild hemorrhagis on intentines. The infected organ is inserted into a pot filled with neutral buffer formalin 10%. Then stained with haematoxyllin-eosin for the microscopic preparation. Microscopic examimination revealed  the presence of spiral bacterial cells on the necrotic liver and kidneys tissue.  Infiltration neutrophils and macrophages was also find in pulmonary and spleen tissue.  Besided that intertubuler edematous were also observed under  microscopic examination. It can be concluded changes associated with Leptospira spp infection can be found in liver and kidney tissue with changes such as necrosis, proliferation of kuffer cells and intertubular edematous. 
LAPORAN KASUS: PATOLOGI BALANTIDIOSIS PADA BABI Purnama, Komang Andika; Kardena, I Made; Berata, I Ketut; Winaya, Ida Bagus Oka; Adi, Anak Agung Ayu Mirah
Indonesia Medicus Veterinus Vol 8 (1) 2019
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (748.518 KB) | DOI: 10.19087/imv.2019.8.1.1

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Balantidium coli (B. coli) adalah salah satu dari parasit protozoa pencernaan yang dapat menginfeksi hewan ternak terutama babi. Hewan yang dijadikan studi kasus adalah seekor babi landrace yang berumur sekitar dua bulan, berasal dari Desa Yeh Gangga Kabupaten Tabanan, Bali. Babi kasus didapatkan dalam keadaan sakit selama tiga hari dengan tanda klinis diare berwarna agak putih dan berlendir, lemas, tidak mau makan, kurus dan akhirnya mati. Perubahan patologi anatomi yang teramati peritonitis, pada kolon dan usus halus mengalami distensi, edema, dan pada mukosa usus perdarahan, terutama pada kolon dan sekum. Pada pemeriksaan histopatologi ditemukan infiltrasi sel radang limfosit dan polimorfonuklear dan ditemukan adanya protozoa B. coli di dalam tunika mukosa sampai tunika sub mukosa kolon. Simpulannya adalah babi terserang penyakit balantidiosis.
PERUBAHAN HISTOPATOLOGI TRAKEA MENCIT JANTAN PASCAPAPARAN ASAP ROKOK ELEKTRIK Wira, Amar; Winaya, Ida Bagus Oka; Adi, Anak Agung Ayu Mirah
Indonesia Medicus Veterinus Vol 7 (4) 2018
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.349 KB) | DOI: 10.19087/imv.2018.7.4.422

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Penelitian ini bertujuan untuk mengetahui perubahan histopatologi trakea mencit (Mus musculus) jantan pascaterpapar asap rokok elektrik (vaping). Penelitian ini menggunakan 24 ekor mencit yang dibagi menjadi dua kelompok perlakuan. Kelompok I (P0) sebagai kelompok kontrol yakni tanpa perlakuan pemaparan asap rokok elektronik dan kelompok II (P1) yang terdiri dari 12 ekor mencit adalah sebagai kelompok perlakuan yang diberi paparan asap rokok elektrik. Penelitian ini menggunakan rancangan acak lengkap (RAL) pola faktorial dengan tiga kali pengambilan sampel minggu ke -1, 2 dan 3 pascaperlakuan. Masing-masing kelompok dikorbankan 4 ekor mencit pada setiap waktu pengamatan. Jaringan trakea diambil dan dimasukkan ke dalam pot yang telah diisi cairan netral buffer formalin 10%, jaringan trakea kemudian diproses untuk pembuatan preparat. Berdasarkan hasil analisis statistik perlakuan tidak berpengaruh nyata P>0,05, begitu pula lama pemaparan juga tidak berpengaruh nyata P>0,05. Hal ini kemungkinan dikarenakan terlalu singkatnya waktu pemaparan dan kandungan nikotin yang rendah pada rokok elektrik.  Berdasarkan pengamatan histopatologi ketebalan mukosa trakea pada kelompok perlakuan lebih tebal dibandingkan dengan kelompok kontrol. Analisis statistik menunjukkan paparan asap rokok elektrik tidak berpengaruh terhadap ketebalan mukosa trakea, namun secara histologi paparan asap elektrik berpengaruh terhadap ketebaan mukosa trakea.
KARAKTERISTIK MOLEKULER VIRUS AVIAN ORTHOAVULAVIRUS 1 GENOTIPE VII YANG DIISOLASI DARI TABANAN BALI (MOLECULAR CHARACTERISTIC OF AVIAN ORTHOAVULAVIRUS 1 GENOTYPE VII ISOLATED FROM TABANAN BALI) Adi, Anak Agung Ayu Mirah; Astawa, I Nyoman Mantik; Wandia, I Nengah; Putra, I Gusti Agung Arta; Winaya, Ida Bagus Oka; Krisnandika, Anak Agung Keswari; Wijaya, Anak Agung Oka
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.964 KB) | DOI: 10.19087/jveteriner.2019.20.4.593

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Newcastle disease (ND) is a very harmful avian disease, endemic in Indonesia and various parts of the world. The causative agent is ND virus or Avian orthoavulavirus 1 (AOAV-1). This virus is an RNA virus with wide genetic variation. Based on the genome length, it can be classified into AOAV-1 Class I and II. Class I are generally avirulent whereas Class II are consist of both virulent and avirulent viruses, currently there are 18 genotypes of the class II. To find out the molecular characteristics of AOAV-1 currently circulating in the field, isolation and identification of viruses from laying hens that was suspected ND from Tabanan Bali in 2017, was performed. The isolated viruses hereafter named as Tabanan1/ARP / 2017. A one-step RT-PCR reaction was carried out to amplify NP, F and HN gene fragments from the virus using three specific pairs of AOAV-1 primers. The obtained nucleotide sequences are then used in phylogenetic analysis. For phylogenetic analysis several strains of AOAV-1 from class II representing genotype I-VII as well as one strain from Class I were accessed from GenBank. From the analysis of the F gene nucleotide sequences, it was found that Tabanan 1 / ARP / 2017 is a genotype VII virus with an amino acid sequence at the F protein cleavage site is 112 R-R-Q-K-R-F117, a typical virulent strain. Phylogenetic analysis using nucleotide sequences NP and HN genes also positioned this isolate in genotype VII. At the nucleotide level, genetic distance with virulent isolates that was isolated in 2007 and 2010 were 8.26% and 1.08% while at the amino acid level were 5.26% and 0.64%. There were found mutations in amino acids at positions 107 and 108 of  F protein. 
PERUBAHAN HISTOPATOLOGI HATI DAN PARU MENCIT PASCAINDUKSI DENGAN ZAT KARSINOGENIK BENZO(A)PIREN Bire, Ienoliski Rohi; Winaya, Ida Bagus Oka; Adi, Anak Agung Ayu Mirah
Indonesia Medicus Veterinus Vol 7 (6) 2018
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.286 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui gambaran histopatologi hati dan paru-paru mencit pascainduksi dengan zat karsinogenik benzo(a)piren. Penelitian ini menggunakan 10 ekor mencit jantan yang dipelihara pada lingkungan serta diet yang sama. Mencit kemudian dibagi menjadi dua kelompok. Kelompok I diinjeksi dengan benzo(a)piren sebanyak 0,3 mg dalam 0,1 mL oleum olivarium secara subkutan sedangkan kelompok II hanya diinjeksi dengan oleum olivarum dengan cara yang sama. Setelah satu bulan, semua mencit dikorbankan. Sampel jaringan hati dan paru dimasukan kedalam NBF 10% untuk proses pemeriksaan histopatologi lebih lanjut. Data pemeriksaan histopatologi hati dan paru kemudian dianalisis secara deskriptif. Hasil yang didapatkan menunjukkan adanya perbedaan gambaran histopatologi hati dan paru mencit antara kelompok I dibandingkan dengan kelompok II. Adapun gambaran mikroskopik yang dominan pada kelompok I adalah: pada hati terjadi kongesti pada vena sentralis serta nekrosis pada sel hepatosit. Pada paru terjadi nekrosis serta adanya penebalan septa alveoli dan infiltrasi sel radang sedangkan untuk kelompok II, tidak terdapat perubahan pada gambaran mikorskopiknya. Berdasarkan hasil penelitian, zat karsinogenik benzo(a)piren dapat menyebabkan terjadinya kongesti pada vena sentralis serta nekrosis pada sel hepatosit serta penebalan pada septa alveoli dan adanya infiltrasi sel-sel radang mononuklear pada paru.
VAKSIN GUMBORO MENYEBABKAN IMUNOSUPRESIF PADA RESPONS PRIMER VAKSIN PENYAKIT TETELO AYAM PEDAGING Kencana, Gusti Ayu Yuniati; Adi, Anak Agung Ayu Mirah; Ardana, Ida Bagus Komang; Mahardika, I Gusti Ngurah Kade
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The variety of Gumboro live vaccine strains (hot, intermediate, and mild) are available inIndonesia. The immunosuppresive effect of these vaccines under field conditions is not known.This research was conducted to determine this devastating effect of such vaccine strains on theimmune response of chickens vaccinated againts Newcastle disease (ND). Sixty chickens werekept separately in five groups (i.e. V1, V2, V3, V4, and K). At the age of seven days, group V1, V2,and V3 were given hot, intermediate, and mild strains of Gumboro live vaccine respectively whilethe other two groups recieved no Gumboro vaccine (V4 and K). At the age of 14 days, all groups,except group K which were kept as a negative control, were vaccinated against ND. The level ofantibody produced in response to ND vaccination was measured in sera collected at day 0, 7, 14,and 21 post ND vaccination using a standard micro-haemaglutination inhibition test. Data of theantibody titers were analyzed using analysis of variance followed by Duncan?s multiple range test.The results showed that all Gumboro vaccine strains still retain its immunosuppressive nature onhumoral immune response in chickens that later vaccinated against ND. The geometric meantiter (GMT) of anti-NDV antibody of group V4 (unvaccinated againts Gumboro) was significantlyhigher than that of group V1, V2, and V3, i.e. groups of chickens that had been given varietystrains of Gumboro vaccines, at the first and second week after ND vaccination (p<0.05). Thedifference of this immunosuppressivenes among variety of Gumboro vaccine strains need furtherclarification.
SUSUNAN NUKLEOTIDA GEN HEMAGGLUTININ-NEURAMINIDASE DARI VIRUS NEWCASTLE DISEASE ISOLAT DENPASAR-03/AK/07 Widiarta, Lutfi; Adi, Anak Agung Ayu Mirah; Kardena, I Made
Indonesia Medicus Veterinus Vol 6 (3) 2017
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.476 KB)

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Virus Newcastle Disease (ND) merupakan salah satu agen penyakit unggas infeksius yang tersebar di Bali dan bersifat endemik. Virus ND merupakan virus dengan materi genetik RNA sehingga memiliki tingkat mutasi yang tinggi. Oleh karena itu evaluasi keragaman genetik virus di lapangan perlu diadakan secara berkala. Tujuan penelitian ini adalah untuk mengetahui susunan  nukleotida gen penyandi protein HN dari Virus ND isolat lapangan asal kota Denpasar. Isolat Denpasar-03/AK/07 diisolasi dari kasus penyakit ND di Denpasar, Bali. Isolasi RNA dilakukan dengan metode Trizol. Fragmen gen penyandi protein HN diamplifikasi dengan RT-PCR. Hasil RT-PCR divisualisasikan pada gel agarosa 1% kemudian produk PCR ini disekuensing di PT. Genetika Science, Jakarta. Untuk melihat jarak genetik dan variasi asam amino, sekuen nukleotida yang didapat kemudian dijajarkan dengan beberapa isolate Virus ND terdahulu yang diakses dari  Gen Bank. Analisis filogenetik  dilakukan dengan menggunakan aplikasi MEGA version 6.0. Dari hasil analisa filogenetik didapatkan bahwa jarak genetik isolate ini dekat dengan isolat Bali-1/07 yakni 2,3 %. Namun terhadap isolat Banjarmasin /010/10, Gianyar /013/10, Kudus /018/10, dan Sragen /014/10 jarak genetiknya adalah 8,5%. Sementara itu dengan isolat Badung-02/AK/14 adalah 9 %. Jarak genetik anatara isolat Denpasar-03/AK/07 dengan isolat vaksin LaSota/46 (genotipe II) adalah 84,8%. Dari analisis susunan asam amino didapatkan bahwa ada variasi antara isolat Denpasar-03/AK/07 dengan isolat terdahulu dari genotipe VII.
GAMBARAN HISTOPATOLOGI KULIT ANJING PENDERITA DERMATITIS (HISTOPATHOLOGICAL FEATURES OF DOG’S SKIN WITH DERMATITIS) Purnama, Komang Andika; Winaya, Ida Bagus Oka; Adi, Anak Agung Ayu Mirah; Erawan, I Gusti Made Krisna; Kardena, I Made; Suartha, I Nyoman
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.116 KB) | DOI: 10.19087/jveteriner.2019.20.4.486

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Complex dermatitis is a disorder of the skin caused by the combination of several agents such as fungi, ectoparasites, bacteria, and metabolic diseases with combined clinical symptoms of primary lesions and secondary lesions. This study aims to determine the histopathology of dog skin with complex dermatitis. There were 15 samples of dogs with complex dermatitis, skin biopsy and histopathology. The results showed that the incidence of complex dermatitis in young dogs was 60%, long-haired 67%, male sex 73%, and domestic race 73%. Macroscopic examination found a change in hyperkeratosis. Microscopically observed that infiltration of polymorphonuclear cells, monomorphonuclear cells and macrofag from moderate to severe in the dermis. In hair follicles and epidermis occured akantosis, and keratin proliferation. There were segment fragments from infectious agents such as Demodex canis, Sarcoptes scabiei, and yeast from Malasezia sp. Other changes were observed in the presence of hydrophic degeneration, necrosis, and ulcers. Dogs suffering from complex dermatitis have chronic infections with more than one infectious agent. Disabilities based on histopathological observation of dogs with complex dermatitis with moderate severity of 33%, and severe 67%. 
GAMBARAN HISTOPATOLOGI USUS HALUS TIKUS PUTIH PASCAPEMBERIAN SARANG SEMUT DAN PARASETAMOL DOSIS TOKSIK Darmawan, I Wayan Eka; Adi, Anak Agung Ayu Mirah; Sudira, I Wayan; Merdana, I Made
Indonesia Medicus Veterinus Vol 7 (5) 2018
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.699 KB) | DOI: 10.19087/imv.2018.7.5.466

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Sarang semut mengandung zat antioksidan yang mampu meminimalisir zat radikal bebas. Tujuan penelitian ini adalah untuk mengetahui efek pemberian sarang semut terhadap gambaran histopatologi usus halus tikus putih yang diberikan parasetamol dosis toksik. Penelitian ini menggunakan 24 ekor tikus putih jantan dengan berat 200-300 gram yang dibagi menjadi empat kelompok perlakuan. Setiap kelompok perlakuan diberi pakan dan minum standar. Tikus pada perlakuan P0 diberikan pakan dan minum standar, P1 diberikan parasetamol 250 mg/kg BB per oral, P2 diberikan  parasetamol 250 mg/kg BB dan ekstrak sarang semut 250 mg/kg BB per oral, dan P3 diberikan ekstrak sarang semut 250 mg/kg BB selama tujuh hari setelah itu diberikan parasetamol 250 mg/kgBB dan ekstrak sarang semut 250 mg/kg BB selama 10 hari per oral. Tikus pada semua kelompok dieuthanasia pada hari ke-18, dengan mengunakan ether. Sampel usus halus diambil untuk diproses menjadi preparat histopatologi dengan pewarnaan rutin Hematoxylin Eosin (HE) dan diperiksa di bawah mikroskop cahaya dengan pembesaran 100-400 kali. Dari hasil pengamatan didapatkan bahwa perubahan yang dominan pada usus halus adalah pendarahan dan nekrosis pada kelompok perlakuan P1, P2 dan P3. Uji Kruskall-Wallis menunjukkan adanya perbedaan yang bermakna pada rerata pendarahan dan nekrosis. Pada lesi pendarahan terdapat perbedaan yang sangat signifikan (P<0,01) antara kelompok kontrol positif (P1) dengan  P2 dan P3. Pada lesi nekrosis terdapat perbedaan yang sangat signifikan (P<0,01) antara kelompok kontrol  positif (P1) dengan  P2 dan P3. Dari penelitian ini dapat disimpulkan bahwa sarang semut dapat memperbaiki kerusakan mukosa usus halus akibat pemberian parasetamol dosis toksik pada hewan coba tikus putih.