I Ketut Mudite Adnyane
Departemen Anatomi, Fisiologi, dan Farmakologi Fakultas Kedokteran Hewan Instititut Pertanian Bogor

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STUDI MIKROANATOMI PANKREAS KODOK LEMBU MENGGUNAKAN METODE PEWARNAAN BAKU DAN IMMUNOHISTOKIMIA (MICROSCOPICAL STUDY OF PANCREAS OF BULLFROG USING CONVENTIONAL AND IMMUNOSTAINING METHODS) Adnyane, I Ketut Mudite; ., Supratikno; Winarto, Adi; Agungpriyono, Srihadi
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Morphology, distribution and relative frequency of endocrine cells in the pancreas Bullfrog (Ranacatesbeiana) were studied using conventional and immunohistochemical methods. Samples pancreas takenfrom ten adult Bullfrogs (five males and five females). In general, pancreas of the Bullfrog consists ofexocrine portion, endocrine portion (Langerhans islets) and ducts. The Langerhans islets were distributedamong the exocrine portion of pancreas. Endocrine cells in the pancreas of Bullfrog were polimorph, round,oval or triangular in shapes with bipolar cytoplasmic granules. Glucagon cells were distributed mainly inthe peripheral, insulin cells in the center while the somatostatin cells in the area between glucagon andinsulin cells of Langerhans islet. The number of the glucagon cells were higher compare to the number ofinsulin and somatostatin cells.
COMPARATIVE MICROANATOMY OF THE LOCAL GOAT AND SHEEP PANCREAS ISLETS WITH A SPECIAL REFERENCE TO THE DISTRIBUTION AND RELATIVE FREQUENCY OF GLUCAGON PRODUCING CELLS Adnyane, I Ketut Mudite; Novelina, Savitri; Sari, Dwi Kesuma; Wresdiyati, Tutik; Agungpriyono, Srihadi
Media Veteriner Vol. 8 No. 1 (2001): Media Veteriner
Publisher : Media Veteriner

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Abstract

Penelitian ini memanfaatkan teknik pewarnaan standardan khusus, impregnasi perak Grimelius, untukmenggambarkan morfologi komparasi pankreas kambing dandomba lokal dengan tinjauan khusus pada distribusi danfrekuensi sel-sel penghasil hormon glukagon pada bagianendokrin pankreas. Pankreas domba mempunyai lobulasiyang lebih jelas daripada pankreas kambing ditandai dengansepta interlobaris yang jelas, tetapi batas antara bagianendokrin (pulau Langerhans) dan bagian eksokrin tidak jelaspada domba. Sebaliknya pankreas kambing mempunyaibagian endokrin yang jelas batasnya dengan bagian eksokrin.Pulau Langerhans tersebar diantara eksokrin pankreas,dengan frekuensi terbanyak didapatkan pada pankreas bagiankanan (head), diikuti bagian kiri (tail) dan tengah (body).Pankreas kambing mempunyai bagian endokrin yang lebihbanyak dibanding dengan pankreas domba. Sel-sel penghasilhormon glukagon pada pankreas berbentuk polimorfik, bulat,oval, segitiga atau seperti tetes air dengan butir-butirsitoplasma yang terletak bipolar. Sel-sel ini berdistribusipada bagian perifer dari pulau Langerhans. Jumlah sel-selglukagon berbanding lurus dengan jumlah pulau Langerhanspada pankreas. Perbedaan yang diamati, mencakupperbedaan morfologis, sebaran serta jumlah pulauLangerhans dan sel-sel glukagon, sangat mungkindisebabkan oleh perbedaan dalam jenis dan pola makankedua hewan tersebut.
STUDI HISTOKIMIA SEBARAN KARBOHIDRAT USUS BIAWAK AIR (VARANUS SALVATOR) Wahyuni, Sri; Zuchri, .; Hamny, .; Jalaluddin, Muhammad; Adnyane, I Ketut Mudite
Acta VETERINARIA Indonesiana Vol. 3 No. 2 (2015): Juli 2015
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1034.948 KB) | DOI: 10.29244/avi.3.2.77-84

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Penelitian ini bertujuan mengetahui sebaran karbohidrat pada usus biawak air dengan metode histokimia. Organ usus dari satu ekor biawak jantan dewasa dikoleksi melalui prosedur perfusi dan selanjutnya difiksasi dengan larutan paraformaldehid 4%. Usus biawak dibagi menjadi enam bagian, yaitu bagian I sampai VI dan selanjutnya diproses menjadi preparat histologi. Deteksi sebaran karbohidrat pada lapisan mukosa usus dilakukan dengan pewarnaan alcian blue (AB) pH 2,5 untuk karbohidrat asam dan periodic acid schiff (PAS) untuk karbohidrat netral. Hasil penelitian menunjukan bahwa sebaran karbohidrat asam ditemukan pada sel goblet pada keenam bagian usus dengan intensitas reaksi sedang (++) sampai kuat (+++), dan tidak ditemukan pada struktur usus lainnya. Sebaran karbohidrat netral ditemukan di seluruh permukaan jaringan usus dengan intensitas reaksi lemah (+) sedangkan intensitas reaksi sedang (++) sampai kuat (+++) ditemukan pada sel goblet. Jumlah sel goblet penghasil karbohidrat asam dan netral pada usus bagian I-IV lebih sedikit (+~++) dibandingkan usus bagian V-VI (+++). Berdasarkan hasil penelitian dapat disimpulkan bahwa sebaran karbohidrat asam dan netral ditemukan diseluruh bagian usus dengan intensitas pewarnaan yang bervariasi. Jumlah sel goblet yang terdeteksi menghasilkan kedua jenis karbohidrat tersebut lebih banyak ditemukan pada usus bagian kaudal.Kata kunci: jaringan usus, sel goblet, karbohidrat asam dan netral, Varanus salvator. (Histochemical Study of Intestinal Carbohydrates Distribution in the Water Monitor (Varanus salvator))The objective of this study was to elaborate the distribution of carbohydrate in intestine tissue of water monitor using histochemical method. Intestinal organ from an adult male water monitor was collected after perfused and subsequently fixed in paraformaldehyde 4%. Intestinal organ was divided to six regions e.g. I to VI and then processed to histological slides. The carbohydrate distribution on the mucosal surface of intestinal tissue was stained with alcian blue (AB) pH 2.5 for detect the acid carbohydrates and periodic acid Schiff (PAS) for the neutral carbohydrates. The results showed that the distribution of acid carbohydrates found in the goblet cells at the I to VI regions with vary intensity of staining reaction with good staining (++) to intense staining (+++), whereas other intestinal structures did not contain the acid carbohydrates. Furthermore, the distribution of neutral carbohydrates was found in the whole intestinal tissue with weak reaction (+), while good staining (++) to intense staining (+++) was appeared in the goblet cells. Additionally, the number of goblet cells containing acid and neutral carbohydrates at the I-IV region was fewer (+~++) than at V-VI region (+++). Conclusion of this study is the distribution of acid and neutral carbohydrates appeared in all regions of the intestinal tissue. The large number of goblet cells that secreting both of carbohydrate type was found at the caudal of intestinal tissue.Keywords: intestinal tissue, goblet cells, acid and neutral carbohydrates, Varanus salvator.
MORFOLOGI KELENJAR LUDAH KAMBING, KUCING DAN BABI: DENGAN TINJAUAN KHUSUS PADA DISTRIBUSI DAN KANDUNGAN KARBOHIDRAT (THE MORPHOLOGY OF SALIVARY GLANDS OF GOAT, CAT, AND PIG: WITH SPECIAL REFERENCE TO THE DISTRIBUTION AND CARBOHYDRATE CONTENT) Adnyane, I Ketut Mudite
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i2.3826

Abstract

This research aimed to compare the morphology and carbohydrate content of parotid andmandibular glands of goat, cat and pig (herbivore, carnivore and omnivore). The microscopic observationwas done using histochemical method with hematoksilin eosin (HE), alcian blue (AB) pH 2.5 andperiodic acid Schiff (PAS) staining methods.The results showed that the parotid and mandibular glandsconsist of acinar cells and ducts. The parotid gland of goat and pig were serous while mixed in the cat.The mandibular gland of goat, cat and pig were mixed type. The acinar of mandibular gland is composedof serous and mucous cells in which the mucous cell predominates. The AB and PAS stain showed weakto high concentration of acid and neutral carbohydrates found in the mucous cells of mandibular gland.However, these carbohydrates were not found in the serous cells of both glands, while acid carbohydratewas found in the parotid gland of cat. The present result suggested that the carbohydrates in the salivaof the goat, cat and pig might be produced mainly by the mucous cells.Keywords: parotid gland, mandibular gland, PAS, AB pH 2.5
MORFOLOGI KELENJAR AKSESORI KELAMIN MUNCAK (MUNTIACUS MUNTJAK MUNTJAK) JANTAN Wahyuni, Sri; Manik, Lidya Elizabeth M.; Agungpriyono, Srihadi; Agil, Muhammad; Yusuf, Tuty Laswardi; Hamny, .; Adnyane, I Ketut Mudite
Acta VETERINARIA Indonesiana Vol. 1 No. 2 (2013): Juli 2013
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (10818.482 KB) | DOI: 10.29244/avi.1.2.80-90

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Penelitian ini bertujuan untuk mempelajari morfologi kelenjar aksesori muncak jantan secara makroanatomi dan mikroanatomi. Seekor muncak jantan dewasa berumur 4-5 tahun dengan bobot badan 19 kg digunakan pada penelitian ini. Muncak terlebih dahulu di-exanguinasi untuk dikoleksi kelenjar aksesori kelaminnya. Untuk memperoleh gambaran mikroanatomi, sampel kelenjar aksesori diproses dengan teknik histologi dan diwarnai dengan pewarnaan hematoksilin-eosin (HE). Hasil pengamatan makroskopis menunjukkan bahwa kelenjar aksesori muncak jantan terdiri atas ampula, duktus deferens, kelenjar prostat, kelenjar vesikularis, dan kelenjar bulbouretralis. Karakteristik histologi kelenjar aksesori muncak adalah ditemukannya kelenjar prostat yang berbentuk pars diseminata dengan kelenjar-kelenjar sekretori tersebar di sekeliling lumen uretra pars pelvina dimana secara makroskopis kelenjar tersebut tidak dapat diamati. Tipe kelenjar sekresi pada ampula, kelenjar vesikularis, dan pars diseminata prostat adalah tubuloalveolar, sedangkan pada kelenjar bulbouretralis tipe tubular. Dapat disimpulkan bahwa morfologi kelenjar aksesori muncak jantan memperlihatkan kemiripan dengan kelenjar aksesori pada ruminansia kecil lainnya seperti kambing, domba, reeves muntjak, dan pampas deer.
PROFIL IMUNOHISTOKIMIA ANTIOKSIDAN COPPER, ZINC-SUPEROXIDE DISMUTASE (CU, ZN, -SOD) PADA GINJAL TIKUS PERINATAL DAN NEONATAL Wresdiyati, Tutik; Adnyane, I Ketut Mudite; Prabandari, Silvia Arin; Sofiawati, Sofiawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.2913

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The aim of the research was to observe the profile of intracellular antioxidant, especially copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the kidney of perinatal and postnatal rats immunohistochemically. A total of twuelve Wistar rats were used for this research and they were divided into four groups that are 18 days (Fe18) and 20 days old fetus (Fe20), one day (N1) and three days neonatus (N3). There are three rats in each groups. Kidney from each group were taken and processed for routin paraffin embedding methods. The tissues sections were observed with Hematoxylin-eosin (HE) and immunohistochemical technique for Cu, Zn-SOD. Cu,Zn-SOD in the kidney were observed qualitatively in medulla, glomerulus dan cytoplasm of tubuli renalis proximalis and tubuli renalis distails, as well as quantitatively in the nucleus of tubuli renalis cells. The results showed that Cu, Zn-SOD increased in the kidney of perinatal rats, 20 days old fetus (Fe20), then it decreased in the tissues of the one days neonatus (N1), then were increased again in the kidney of three days neonatus (N3) group rats.
PENYUNTIKAN HUMAN WHARTON’S JELLY MESENCHYMAL STEM CELLS TERHADAP PERBAIKAN FUNGSI TESTIS PADA TIKUS TUA FISIOLOGIS (HUMAN WHARTON’S JELLY MESENCHYMAL STEM CELLS INJECTION AMELIORATE TESTICULAR FUNCTION ON PHYSIOLOGICAL AGING MALE RATS) Fitrianto, Alif Iman; Utami, Adkhilni; Astini, Wining; Dwijayanti, Adisti; Suyatna, Frans Dhyanagiri; Yaprianto, Kelvin; Bachtiar, Indra; Adnyane, I Ketut Mudite; Boediono, Arief
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The most common therapy on men who suffered fertility decline due to aging was called ?T Therapy?, but that?s therapy has long-term risks of sexual dysfunction, metabolic syndrome, prostate, and cardiovascular system. Stem cells are an alternative therapy can be used for ameliorate testicular fuction because of their ability to differentiate into various cell types. The aim of this study was to evaluate the injection of hWJ-MSC in physiologic aging male rats on testicular function. This study was used 3 young male rats (8-12 weeks) and 6 physiological aging male rats (22-24 months) which divided into 3 groups, (i) the young rats, (ii) physiological aging male rats, and (iii) physiological aging male rats that injected with hWJ-MSCs. The young rat group did not give any treatment, physiological aging male rats received NaCl (0.9%) 0.4 mL, and the treatment group received 1x106 cells/kg BW of hWJ-MSCs. The observations were performed on the macroscopical and histological analysis. The result indicates that the younger group had the lowest body weight (154.6 g) and the percentage of the testis weight on the body weight was highest (2.2%) compared to the other groups (P>0.05). The physiological aging rats group had the smallest tubule (9726.9 ìm2) with a largest interstitial area (1117.1 ìm2) compared the other groups (P>0.05). After injection of hWJ-MSC, the tubule area became wider followed by narrowing of the interstitial area (P>0.05). The difference in the body weights is due to the different age of the rat. Improvement of tubule area and interstitial area due to the ability of hWJ-MSCs to improve spermatogenic cells within the tubule. Injection of hWJ-MSCs has been shown to increase fertility in aging rats. 
MORFOLOGI KELENJAR ANAL MUSANG LUAK JANTAN (PARADOXURUS HERMAPHRODITUS) Maha, Inggrid Trinidad; Novelina, Savitri; Adnyane, I Ketut Mudite
Acta VETERINARIA Indonesiana Vol. 7 No. 2 (2019): Juli 2019
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1293.737 KB) | DOI: 10.29244/avi.7.2.33-41

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Penelitian ini bertujuan untuk mempelajari karakteristik morfologi kelenjar anal musang luak jantan secara makroskopis maupun mikroskopis.Organ kelenjar anal dari satu ekor musang luak  (Paradoxurus hermaphroditus) jantan digunakan dalam penelitian ini. Musang luak jantan memiliki sepasang kelenjar anal yang berbentuk bulat terletak di bagian ventrolateral anus. Masing-masing kelenjar anal memiliki saluran eksretorius sekretori yang terletak dorsolateral di bagian zona kutaneus anal kanal. Pengamatan mikroskopis tampak kelenjar sebaceous dan kelenjar keringat apokrin di dinding kantung anal.  Hasil sekreta kelenjar anal terkumpul dalam sebuah kantung anal yang akan bermuara di anal kanal.
AH-19 THE HEAVY METAL DEPOSIT VISUALIZATION ON ORGAN TISSUES AFTER SLAUGHTERING ON BEEF PRODUCTION Winarto, Adi; Adnyane, I Ketut Mudite; Pamungkasari, Yohana Paula Prihatmi
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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The Heavy Metal Deposit Visualization on Organ Tissues after Slaughtering on Beef Production
KARAKTERISTIK FISIK DAN MIKROSTRUKTUR OTOT SEMITENDINOSUS PADA SAPI LOKAL DAN SAPI IMPOR (PHYSICAL AND MICROSTRUCTURE CHARACTERISTICS OF SEMITENDINOSUS MUSCLE IN LOCAL CATTLE AND IMPORTED COWS) Safitri, Aldina; Priyanto, Rudy; Adnyane, I Ketut Mudite; Nuraini, Henny
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2018.19.4.488

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Semitendinosus muscle is widely used as raw material for traditional culinary in Indonesia. Studies on local beef cattle potency have been widely conducted, however there is limited information on physical and microstructures characteristics of semitendinosus muscle.  This study aimed to examine physical and microstructures characteristics of semitendinosus muscle from different breeds of local and import beef cattle.  This study used semitendinosus muscle from angus cross cattle, bali cattle, brahman cross cattle, PO cattle, and simmental X PO cattle, with age ranging from 18-30 months (I1-I2).  The results showed that semitendinosus muscle of local breed cattle could meet the criteria of physical characteristics of consumer demand in Indonesia. Based on perimysium thickness and fascicle area of the cross section of semitendinosus muscle, angus cross cattle and bali cattle had softer muscle texture compared to the other breeds.