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Evaluation of Sucrose for In Vitro Germination and Growth of the Entomopathogenic Fungus Beauveria bassiana (BALSAMO) VUILLEMIN and Paecilomyces sp. (DEUTEROMYCETES, MONILIALES) Afandhi, Aminudin; Chailani, Siti Rasminah; Agustiawati, Agustiawati
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

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Abstract

AbstractIn order to examine some possible effects of granulated sugar and sucrose in B. bassiana conidiasuspension, we examined the effects of sucrose on germination dan growth of Beauveria bassiana and Paecilomyces sp. in vitro. We hoped to provide some information relevant to mass production methods for B. bassiana and Paecilomyces sp. The conidia germination and growth of B. bassiana and Paecilomyces sp. were increased by addition of sucrose in medium. The addition sucrose maybe a useful carbohydrate source for use in mass production and carrier ingredient into conidiasuspension as bioinsecticide.Keywords: Beauveria bassiana, Paecilomyces sp., conidia germination, mycelial growth, sucrose
MULTIPLE TACTICS OF INTEGRATED PEST MANAGEMENT FOR THRIPS ON STRAWBERRY PLANTATION Mudjiono, Gatot; Prasman, Yahya; Afandhi, Aminudin
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i2.520

Abstract

In management of thrips on strawberry, farmers usually use synthetic insecticides. Synthetic insecticide is not effective to control thrips and produce residual effect on crops. Insecticidal residue is dangerous for human, natural enemies and environment widely. Therefore, it is necessary to find an alternative thrips pest control on strawberries, through integrated pest manage-ment (IPM) implementation. Research was aimed to determine the effect of IPM technology for Thrips sp. on strawberry plantation. Research was conducted on strawberry plantation by comparing IPM and conventional plots, in Kusuma Agrowisata Batu from April to July 2009. Variables were used such as the number of larvae, adult population and damage intensity caused by Thrips sp., and fruit production. Data was analyzed by t-test on 5%. The results showed that the average larva and adult population of Thrips sp. on IPM plot were higher than conventional plot, 1.12 and 0.50 for larva and 0.87 and 0.27 for adults respectively. The damage intensity of strawberry crops on IPM plot was higher than conventional plot, 11.69 and 5.98% respectively. Based on average fruit production, IPM plot was higher than conventional plot, 48.32 g and 39.24 g respectively.
Efektifitas Pestisida Biologis Bacillus Cereus dan Bacillus Megaterium. sebagai Pengendali Spodoptera litura Fabr (Lepidoptera: Noctuidae) Agastya, I Made Indra; Afandhi, Aminudin; Aini, Luqman Qurata
Jurnal Penelitian Pertanian Terapan Vol 17, No 2 (2017)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.936 KB) | DOI: 10.25181/jppt.v17i2.294

Abstract

This research was conducted in vitro in the laboratory. Studies conducted in the Laboratory of Bacteriology Department of Plant Pests and Diseases of the Faculty of Agriculture, University of Brawijaya, on the effectiveness of the bacteria Bacillus cereus and Bacillus megaterium as biological pesticides controlling Spodoptera litura. The objective of this study was to determine the effectiveness of the bacteria Bacillus sp as biological control. Effectiveness pesticide was measured by testing the incubation period and mortality in larvae of S. litura instar 3. This study used a completely randomized design (CRD), conducted observations every 6 hours until the larvae dead. The results showed that the percentage of mortality of S. litura reached 94.66% compared to the control, while the incubation time of the bacteria B. cereus cause disease until 29.84 hours. B. cereus and B. megaterium have the ability to incubate third instar larvae of S. litura up to 29.84 hours and caused the death of larvae up to 94.66%. Keywords: Biological pesticides, Bacillus cereus, Bacillus megaterium, entomopatogen, Spodoptera litura
Types of Insects in Organic and Semi-Organic Citrus Plantation Pora, Misykat Sulthana; Leksono, Amin Setyo; Afandhi, Aminudin
Indonesian Journal of Environment and Sustainable Development Vol 7, No 2 (2016)
Publisher : Graduate Program

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Abstract

Using pesticide within semi-organic plantation system results in declining number and type of insects. Within the ecosystem, insect functions as herbivore, carnivore, detrivore and pollinator. The purpose of the study is to describe the structure and function of insect composition in organic and semi-organic orange plantation. The study used the blue and yellow trap pan method. The trap pan is installed one meter above the ground. 10 settlers are needed for 5 times of replication. The findings show that there are 1910 insects that belong to 5 orders and 24 families; formicidae family from hymenopetra is the most frequently found insect in the plantation. The Shannon Diversity Index showed difference between the organic and semi-organic plantation.The findings suggested that type of insects in the organic and semi-organic plantation is related to type of technology in each of the orange plantation.Keywords:  insect, organic, type
Analysis of Infiltration Well Dimensional in Kedungkandang District, Malang City, Indonesia Jifa, Azarine Nabila; Rachmansyah, Arief; Afandhi, Aminudin
Indonesian Journal of Environment and Sustainable Development Vol 9, No 1 (2018)
Publisher : Graduate Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1047.693 KB) | DOI: 10.21776/ub.jpal.2018.009.01.06

Abstract

Kedungkandang District is one of the fastest growing areas in Malang City and become a urban area. The implication this development is dcreasing of urban open space area by 1 - 2% per year, and followed by increased surface flow during rain. Infiltration well is one of the efficient rainwater utilization to reduce run-off. In Kedungkandang district three are different soil types which affects the permeability coefficient value for determination the dimension of infiltration well. This study aimed to determine the dimension of infiltration wells suitable for application individual infiltration wells for housing type 36, 45, 54, 70, 90 and 110.  In order to determine dimension of infiltration wells would be used, there were some analysis done: hydrology and soil permeability coefficient. The conclusion from this research was the bigger the house size, it would need a bigger dimension of infiltration well as well. From the three types of soil in Kedungkandang, the houses in alluvial fan ground type and tuff volcanic soil require a smaller size of infiltration wells than the houses located in Buring volcano mountain sediments.Key words: infiltration well, run-off 
The Influence of The Environment And Mother’s Behaviour Towards Toddlers’ Diarrhea in Banjarbaru, South Kalimantan Faisal, Nopy; Sriwahyuni, Endang; Afandhi, Aminudin
Indonesian Journal of Environment and Sustainable Development Vol 7, No 2 (2016)
Publisher : Graduate Program

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Abstract

Diarrhea is a disease that causes high fatality in toddlers all over the world. 1.5 million children die each year due to suffering from diarrhea. Diarrhea is still a major health problem in Banjarbaru, it is included in the top 10 diseaseas in every year. Bad water quality, bad sanitation facility and mother’s unhygienic behaviour are the major cause of diarrhea in toddlers. This research aims to analyze the influence of the environment quality and mother’s behaviour towards toddlers’ diarrhea in Banjarbaru. The research variable consists of the well quality, family latrines, wastewater disposal facility, bacteriological quality of the well water, mother’s clean and healthy living behaviours (PHBS or Perilaku Hidup Bersih dan Sehat) as well as the incidence of diarrhea in toddlers. It is conducted in Landasan Ulin Public Health Center and North Banjarbaru Public Health Center with total sample of 50 toddlers. The data analysis used is univariate, bivariate and multivariate. The results showed that the mother’s PHBS (p = 0.005; OR = 13,077) and the bacteriological quality of the well water (p = 0,041; OR = 6,966) became the most influential variables of this toddlers’ diarrhea. The activities that are required in order to control the diarrheal diseases in Banjarbaru are change the mothers’ behaviour in the prevention of toddlers’ diarrhea, improve the environment quality with Community Lead Total Sanitation Program (STBM or Sanitasi Total Berbasis Masyarakat), making a Local Regulation for wastewater management, an enhancement of officers for prevention and control of diarrhea and awarding donations for low-income citizens.Key words: diarrhea, mother’s behaviour, sanitation, water, 
Correlation of Soil Environmental to Diversity the Entomopathogenic Fungi Handoko, Rose Novita Sari; Afandhi, Aminudin; Leksono, Amin Setyo
Indonesian Journal of Environment and Sustainable Development Vol 8, No 1 (2017)
Publisher : Graduate Program

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Abstract

Ecosystem rice fields that have high diversity, able to control the pest. Habitat entomopathogenic fungi in the soil have been examined on cabbage plants but has not been reported in the rice field. The study was conducted through surveys of crops and paddy fields by the application of IPM in Kasembon Malang. A total of 5 points soil samples were determined diagonally used in this study. Isolation of entomopathogenic fungi from the rhizosphere of paddy is done by plate dilution method. Entomopathogenic fungi were identified to genus level by observing the macroscopic and microscopic characteristics. Kochs postulates done on Tenebrio molitor, then observe the symptoms and mortality of Spodoptera litura time pathogenicity test. The results showed that the genus of entomopathogenic fungi in the rhizosphere of rice is Penicillium sp., Aspergillus sp. and has not been identified. Chemical analysis of soil for pH is 4.00 to 5.00. Soil organic matter is 1.89% to 3.20%. Keywords: diversity, entomopathogenic fungi, integrated pest management, rhizosphere
The Utilization of Skipjack Tuna (Katsuwonus pelamis) Availability in Prigi Sustainable Fisheries Management, East Java Genti, Jonathan Graydam; Marsoedi, Marsoedi; Afandhi, Aminudin
Indonesian Journal of Environment and Sustainable Development Vol 7, No 2 (2016)
Publisher : Graduate Program

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Abstract

Skipjack tuna is one of the fishery commodities which has high economy value due to the high demand and high prices in the export commodities. The purpose of this research is to identify and analyze the potential resources of tuna and also determine its alternative management in Prigi Fishing Port (Pelabuhan Perikanan Nusantara Prigi). This research was carried out at Prigi Fishing Port, Trenggalek, East Java from February to April 2016. The method used in this research was descriptive method. The data analysis methods were the standardization of the fishing gear, the analysis of catch per unit effort (CPUE), total allowable catch (TAC), and the skipjack tuna fishery management concept at Prigi Fishing Port. The fishing gears which were used to catch skipjack tuna are payang seine, trolling line and purse seine. However, the standard fishing gear used to catch the skipjack tuna was trolling line. CPUE analysis results were obtained from the CPUEstd average value in 2005-2014 as many as 4.79 kg/unit. The allowed amount of the skipjack tuna fish farming was 3,028 tons/year and the result in 2014 was 460.82 tons, means that the skipjack tuna utilization was still under fishing. Meanwhile, the fishing attempt of 224 units was exceeding because it has passed the optimum limit of fishing by 102 units. The results of the study recommended several policies of the skipjack tuna management which are: (1) the amount limitations of the fish farming; (2) the management of the fishing attempts; and (3) the regulation of the fish farming closing season.Keywords: fisheries management, skipjack tuna (Katsuwonus pelamis), Prigi Fishing Port, TAC
Environmental Friendly Analysis on Fishing Gear of Trammel Net in Cilacap, Central Java Sutriyono, Sutriyono; Marsoed, Marsoed; Afandhi, Aminudin
Indonesian Journal of Environment and Sustainable Development Vol 8, No 1 (2017)
Publisher : Graduate Program

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Abstract

The purpose of this study was to analysis about the composition of the catch, the selectivity of fishing gear and fishing gear level of friendliness to the environment. The data collection is done by experimental fishing.  Selectivity analysis applied by  Shannon diversity index and Simpson dominance index. Analysis of scoring was used to rate the friendliness of fishing gear on the environment. The main target of the catch composition (33.93%) and the by catch (66.07%) of the catch species diversity index was 2,16 to 2,78 (high diversity index; the selectivity of fishing gear is low). Dominance index was 0,36 to 0,50 (did not happen dominance species catches). The total value of the scoring is based on the criteria of degree of friendliness of fishing gear on the environment is the 26 included in the category of less environmentally friendly fishing gear. The catch is dominated by small fish. If it lasts for a long time and continuously, it can adversely affect the conservation of fish resources. It should be a mechanism for management systems such as open and close an area to fishing operations or modifications to fishing gear mechanism which can reduce the yield of bycatch and small ones.Keywords: composition, diversity, dominance and environmentally friendly, selectivity
Distribution of the Endophytic Fungi in Apple Leaves Afandhi, Aminudin; Choliq, Fery Abdul; Anggrilika W.S., Havinda; Tarno, Hagus
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.1563

Abstract

Endophytic fungi have roles to protect the plant from herbivorous insects and diseases, and to support the absorption process of nutrients needed by plants for photosynthesis. Related to the important role of endophytic fungi, research was aimed to identify fungal endophytes associated with young, mature and old leaves on apple and to evaluate the effect of leaf ages on the abundance and diversity of endophytic fungi. The research was conducted in Biological Control Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya from May to September 2016. Apple leaves sampling was done diagonally, and taken from nine apple trees. Based on macroscopic and microscopic characteristics, 38 isolates were identified. In the old apple leaves, it obtained 17 isolates that consisted of 5 genera, mature leaves obtained 14 isolates consisted of 6 genera, and young leaves 7 isolates obtained consists of 2 genera. The mature leaves have highest abundance and diversity of endophytic fungi compared to young and old leaves. Endophytic fungi apple leaf dominated by Aspergillus. Mature and young leaves were different from old one based on Bray-Curtis similarity.