Muhammad Agil
Bagian Reproduksi dan Kebidanan Fakultas Kedokteran Hewan Institut Pertanian Bogor

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DETEKSI UMUR PUBERTAS MUNCAK (MUNTIACUS MUNTJAK MUNTJAK) BETINA BERDASARKAN ANALISIS METABOLIT ESTROGEN DAN PROGESTERON PADA FESES (THE AGE OF PUBERTY DETECTION IN FEMALE BARKING DEER (MUNTIACUS MUNTJAC MUNTJAC) BASED ON FAECAL ESTROGEN AND PROGESTERONE A Pudjirahaju, Asri; Supriatna, Iman; Agungpriyono, Srihadi; Agil, Muhammad
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Knowledge and information about the age of puberty in muntjac (Muntiacus muntjac muntjac) isindispensable to the interests of females breeding in conservation efforts. The aims of this study were todetermine the age of puberty and age at first mated females muntjac kept in captivity through the analysisof estrogen and progesterone metabolites in feces. This study used 155 fecal samples that were collectedfrom three female muntjacs. Sample collection was began when muntjac aged three months, four monthsand six months. Total of 10-20 g fecal samples were collected every 2-4 days. Analysis of steroid hormonemetabolites was performed by using enzyme immunoassay (EIA) method with specific antibodies.Determination of the age of puberty was based on the appearance of the first time estrus and ovulation,which was indicated by the appearance of the highest estrogens secretion, on hormone metabolites profile.Hormone metabolites data then were tabulated in the average and standard deviations were presentedwith graphs and analyzed descriptively. The results showed that the age of puberty detection based onanalysis of the estrogens and progesterone metabolite in the feces can be applied in muntjac. Muntjacfemales kept in captivity flats reached puberty at age 5±1 month or 4-6 months range. It is recommendedthe first mated in the muntjac is at least after the female experienced two period of oestrous or has reachedat age of six months.
THE EFFECTIVENESS OF PROGESTERONE HORMONE OR PGF2α AND HCG APPLICATION TO INCREASE REPRODUCTIVE EFFICIENCY OF POSTPARTUM OF ANESTRUS PE GOATS Syawal, Muhammad; Yusuf, Tuty L; Agil, Muhammad
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.7678

Abstract

This study aimed to improve reproductive condition of postpartum anestrus of Etawa crossbreed (PE) goats using PGF2? hormone or progesterone (controlled internal drug release/CIDR-G) and improve pregnancy success from artificial insemination (AI) with human chorionic gonadotrophin (hCG) hormone administration. Administration of progesterone increased estrus by 100%, which was higher than the 70% produced from prostaglandin F2 alfa (PGF2?) administration. Estrus onset in CIDR group (35.80±13.33 hours) was faster than PGF2? group (45.6±9.2 hours). Clinical signs of vulva (redness and swollen) were more intense in CIDR-G group (80% and 70%, respectively) compared to PGF2? group (70% and 57%, respectively). Estrus duration in PGF2? group (52.17±3.34 hours) was longer than CIDR-G group (49.61±3.56 hours). Pregnancy success in group given hCG was 66.7% while in non-hCG group was 50%. In conclusion, CIDR-G and PGF2? administration is effective to induce estrus of postpartum anestrus PE goats. Administration of hCG at the onset of estrus is effective to improve pregnancy success.
ANATOMI DAN GAMBARAN ULTRASOUND ORGAN REPRODUKSI SELAMA SIKLUS ESTRUS PADA KUDA GAYO BETINA (ANATOMY AND ULTRASOUND IMAGING OF REPRODUCTIVE ORGANS OF GAYO MARES DURING ESTROUS CYCLE) Melia, Juli; Agil, Muhammad; Supriatna, Iman; Amrozi, Amrozi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5026

Abstract

The present study examines anatomy of Gayo mare reproductive organs. This study used three sample of Gayo mare reproductive organs (n= 3) for observation of morphology and morphometric of the mare reproductive organs. The ovarium was fixed in 4% paraformaldehyde solution then followed by histological method and stained using hematoxylin and eosin (HE) and Masson?s trichome (MT). Three mares were observed for diameter and changes overview of uterus during estrous cycle in real time using ultrasound. The results showed that, in general, the anatomy of Gayo mare?s reproductive organs was similar to other mares, but smaller in morphometry. The total length of the Gayo mare?s reproductive tract from labia to apex cornua was 48.00±1.00 cm. Weight of Gayo mare?s left ovary was 19.07±7.70 g and the right was 24.43±0.83 g. Histologically, there was no difference between Gayo mare?s structure and other mares. In cortex uteri there were some follicles surrounded by capillary, various development stages of follicles, healthy follicles, atretic follicle, and corpus albican; while in medulla there were a lot of connective tissues. Ultrasound of the uterus showed the change in diameter during estrous cycle with the largest diameter of corpus uteri was 4.43±0.10 cm in horses with estrous cycle of 21 days and 6.30±0.93 cm in horses with 24 days estrous cycle. In conclusion, the morphometry of Gayo mare reproductive organs are smaller than the other horses and there are differences in diameter of the uterus during the estrous cycle due to the changes of endometrium thickness.
MORFOLOGI KELENJAR AKSESORI KELAMIN MUNCAK (MUNTIACUS MUNTJAK MUNTJAK) JANTAN Wahyuni, Sri; Manik, Lidya Elizabeth M.; Agungpriyono, Srihadi; Agil, Muhammad; Yusuf, Tuty Laswardi; Hamny, .; Adnyane, I Ketut Mudite
Acta VETERINARIA Indonesiana Vol. 1 No. 2 (2013): Juli 2013
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (10818.482 KB) | DOI: 10.29244/avi.1.2.80-90

Abstract

Penelitian ini bertujuan untuk mempelajari morfologi kelenjar aksesori muncak jantan secara makroanatomi dan mikroanatomi. Seekor muncak jantan dewasa berumur 4-5 tahun dengan bobot badan 19 kg digunakan pada penelitian ini. Muncak terlebih dahulu di-exanguinasi untuk dikoleksi kelenjar aksesori kelaminnya. Untuk memperoleh gambaran mikroanatomi, sampel kelenjar aksesori diproses dengan teknik histologi dan diwarnai dengan pewarnaan hematoksilin-eosin (HE). Hasil pengamatan makroskopis menunjukkan bahwa kelenjar aksesori muncak jantan terdiri atas ampula, duktus deferens, kelenjar prostat, kelenjar vesikularis, dan kelenjar bulbouretralis. Karakteristik histologi kelenjar aksesori muncak adalah ditemukannya kelenjar prostat yang berbentuk pars diseminata dengan kelenjar-kelenjar sekretori tersebar di sekeliling lumen uretra pars pelvina dimana secara makroskopis kelenjar tersebut tidak dapat diamati. Tipe kelenjar sekresi pada ampula, kelenjar vesikularis, dan pars diseminata prostat adalah tubuloalveolar, sedangkan pada kelenjar bulbouretralis tipe tubular. Dapat disimpulkan bahwa morfologi kelenjar aksesori muncak jantan memperlihatkan kemiripan dengan kelenjar aksesori pada ruminansia kecil lainnya seperti kambing, domba, reeves muntjak, dan pampas deer.
FAECAL GLUCOCORTICOID MEASUREMENT AS INDICATOR STRESS IN WILD CRESTED MACAQUES (MACACA NIGRA): THE IMPORTANCE OF VALIDATION AND SAMPLE PROCESSING TECHNIQUES Gholib, .; Nugraha, Taufiq P.; Agil, Muhammad; Supriatna, Iman; Purwantara, Bambang; Engelhardt, Antje
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Monitoring of physiological stress in wild and/or endangered animals living in their natural habitat can be generated via measuring faecal glucocorticoid metabolites (FGCMs) through enzyme immunoassay technique. However, a careful validation of each enzyme immunoassays (EIAs) and methodological issues such as samples preservation, and extraction procedures must be a significant concern and validated because  it may influence FGCMs levels. In this present study, the aim was to carry out a biological validation to find reliable EIAs that could be used for non-invasive measurement of glucocorticoid levels in crested macaques. Moreover, we compared three different techniques of preservation: lyophilisation, oven drying and field extraction and three different solvents for extraction: 80% methanol, 90% pure ethanol and 90% commercial alcohol (isopropyl alcohol) to find the simple and practical techniques for sample processing. For hormone analysis, we colIected 189 faecal samples from wild crested macaques living in the Tangkoko-Batuangus Nature Reserve, North Sulawesi, Indonesia. The results show that biological validation can be used to validate measurement of faecal glucocorticoid which 3?,11?-dihydroxy-etiocholanolone was the most reliable EIAsfor this species. Different preservation and solvent extraction were not significantly influenced levels of FGCMs (p 0.05). In conclusion, Our study highlights the importance of biological validation of FGCMassays and presents practical techniques for the non-invasive monitoring of physiological stress in crested macaques.
REPEATED FREEZE-THAW CYCLES BUT NOT SHORT-TERM STORAGE OF FECAL EXTRACTS AT AMBIENT TEMPERATURE INFLUENCE THE STABILITY OF STEROID METABOLITE LEVELS IN CRESTED MACAQUES Gholib, Gholib; Agil, Muhammad; Supriatna, Iman; Purwantara, Bambang; Heistermann, Michael; Engelhardt, Antje
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i2.6830

Abstract

The objective of this study was to examine the effects of repeated freeze-thaw cycles and short-term storage of fecal extracts at ambient temperature on the stability of fecal glucocorticoid (fGCM) and estrogen metabolite (fEM) levels from crested macaques.In total 100 aliquots of fecal extracts from fecal samples collected from female crested macaques (Macaca nigra) living at the Tangkoko-Batuangus Nature Reserve, North Sulawesi were used. We performed two different experiments: (1) An experiment to investigate if levels of fGCM and fEM measured from fecal extracts that were exposed to two, four, six and eight repeated freeze-thaw cycles (test groups) differ to control samples (i.e. fecal extracts always stored frozen); (2) An experiment to evaluate whether storing fecal extracts at ambient temperature for two, four, six, and eight days (test groups)affects the levels of fGCM and fEM compared to the control group (i.e. fecal extracts frozen immediately).Results showed that hormone levels were significantly increased (P 0.05) after four freeze-thaw cycles for fGCM and after eight freeze-thaw cycles for fEM. By contrast, there was no significant difference (P 0.05) in levels of fGCM and fEM between the test groups and the control group in fecal extracts stored at ambient temperature. In conclusion, our data show that more than two and six repeated freeze-thaw cycles should be avoided when measuring fGCM and fEM in crested macaque fecal extracts, respectively. We also demonstrate that storing fecal extracts at ambient temperature is possible for at least 8 days without taking a risk of affecting the stability of fGCM and fEM levels.
VALIDASI ANALITIK KIT ELISA KOMERSIAL UNTUK MENGUKUR METABOLIT ESTROGEN DAN PROGESTERON PADA FESES TARSIUS (TARSIUS SPECTRUM) Hidayatik, Nanik; Yusuf, Tuty Laswardy; Agil, Muhammad; Iskandar, Entang; Sajuthi, Dondin
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.547 KB) | DOI: 10.29244/avi.6.1.1-7

Abstract

Penentuan status reproduksi pada satwa liar atau satwa yang ditangkarkan merupakan faktor yang sangat penting dalam manajemen pengembangbiakan satwa. Evaluasi metabolit hormon estrogen dan progesteron secara non-invasive dari sampel feses untuk memonitor fungsi reproduksi telah dilakukan sejak lama pada beberapa spesies mamalia. Validasi asai pada Tarsius belum pernah dilaporkan sehingga validasi asai merupakan hal yang sangat penting sebelum digunakan dalam studi karena metabolit steroid bersifat spesifik spesies. Tujuan penelitian ini adalah melakukan validasi analitik kit enzyme linked immunoassay (ELISA) komersial untuk menganalisis metabolit hormon estrogen dan progesteron pada feses T. spectrum. Uji paralelisme dilakukan pada asai DRG® estradiol (E2), estron (E1), dan progesteron (P4) dengan pengenceran bertingkat (1:2?1:128) ekstrak feses dari beberapa status reproduksi yang berbeda  pada Tarsius yang dibandingkan dengan kurva standar dari masing-masing asai.  Hasil uji paralelisme terhadap kit DRG® estron menunjukkan hasil yang tidak paralel. Dari uji paralelisme DRG® estradiol dan progesteron, didapatkan hasil kurva sampel dengan standar yang tidak konsisten. Hanya ditemukan satu dari lima kurva sampel yang diuji yang paralel dengan kurva standar asai DRG® estrogen dan progesteron. Berdasarkan hasil tes paralelisme tersebut, kit komersial ELISA DRG® estron, estradiol, dan progesteron tidak dapat digunakan untuk mengukur metabolit  estrogen dan progesteron pada feses T. spectrum.
MORFOLOGI DAN MORFOMETRI PERTUMBUHAN RANGGAH VELVET MUNCAK JANTAN (MUNTIACUS MUNTJAK MUNTJAK) Wahyuni, Sri; Agungpriyono, Srihadi; Agil, Muhammad; Yusuf, Tuty Laswardi
Jurnal Kedokteran Hewan Vol 5, No 1 (2011): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i1.419

Abstract

Penelitian ini bertujuan menggambarkan morfologi pertumbuhan ranggah velvet (RV) dua ekor muncak jantan (M1 dan M2) yang dipelihara di kandang penelitian secara terpisah. Tanggal lepas ranggah dan hari pertama percabangan RV serta awal pengelupasan kulit velvet diamati dan dicatat. Pengukuran meliputi panjang dan diameter ranggah velvet utama (RVU) dan ranggah velvet cabang (RVC) dexter et sinister, serta durasi pertumbuhan RV. Rataan ± SD panjang dan diameter maksimum RVU dan RVC muncak M1 adalah 148,25±1,12 mm; 46,26±0,68 mm (panjang), 19,21±2,35 mm; 9,12±0,21 mm (diameter) dengan durasi pertumbuhan 104 hari; sedangkan muncak M2 berturut-turut adalah: 146,44±1,58 mm; 46,94±1,97 mm (panjang); 13,65±1,27 mm; 8,49±0,29 mm (diameter) dengan durasi pertumbuhan 98 hari. Tidak terdapat perbedaan tahapan pertumbuhan RVU dan RVC antara muncak M2 dan M1, namun berbeda pada ukuran RV dan durasi pertumbuhannya. Muncak M2 memasuki ranggah keras setelah pengelupasan kulit velvet lebih cepat dibandingkan M1. Dapat disimpulkan bahwa ukuran pertumbuhan RV berkorelasi erat dengan faktor umur, berat badan, dan postur tubuh muncak jantan.
VALIDASI KIT ENZYME-LINKED IMMUNOSORBENT ASSAY KOMERSIAL UNTUK ANALISIS HORMON ESTRADIOL DAN PROGESTERON DARAH KAMBING KACANG (VALIDATION OF COMMERCIAL ENZYME-LINKED IMMUNOSORBENT ASSAYKIT FORANALYSIS OF ESTRADIOLANDPROGESTERONE HORMONE IN BLOOD OF KACANG Setiadi, Dedi Rahmat; Supriatna, Iman; Agil, Muhammad
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to evaluate the capability of two human commercial enzyme-linkedimmunosorbent assay (ELISA) kits(DRG International Inc.,Germany and GBC Taiwan) for measuringestradiol (E2) and progesterone (P4) in plasma of kacang goat. Three healthy and non pregnant femalekacang goats aged 2-3 years with regular estrous cycles were used in this study. Blood samples werecollected from the jugular vein using a 21G venoject every two days and it was intensified every day duringthe period before heat. Collected plasma were stored at -20ºC until the analysis. Capability validationwas conducted by measuring accuracy (parallelism test), sensitivity and precision.Parallelism test usingDRG commercial kit showed that sample curvewas parallel with standard curve of E2 and P4. In contrastit was not parallel with standard curve of GBC commercial kit. Sensitivity was measured from the lowesthormones concentration of E2 and P4 at 90% binding that were 25 pg/ml and 0.14ng/mL in DRG kit, whilein GBC kit were 5 pg/mL of E2 and 0.2 ng/mL of P4, respectively. Coefisien of variation of intra- andinterassay for both ELISA commercial kits were less than10%. It can be concluded that DRG commercialELISA kit E2 and P4 can be used to analyse female kacang goat blood plasma, while GBC commercialELISA kit E2 and P4 are not recommended.
FA-3 PRODUCTION TRAIT OF CROSSBREED CATTLE AND REPRODUCTIVE DISORDERS IN BRAHMAN CROSS (BX) BREEDING PROGRAM AT PT LEMBU JANTAN PERKASA Pardede, Berlin Pandapotan; Tamba, Bakti; Sutrisnak, Sutrisnak; Wisana, I Ketut Karya; Rahardjo, Harianto Budi; Agil, Muhammad; Yusuf, Tuty L
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

The feedlot industry in Indonesia has developed very rapidly, along with the increase in meat consumption. Brahman Cross (BX) is an imported beef cattle from Australia which is widely used by feedloters as broodstock. Feedloter chooses BX cattle because besides the price is quite cheap, this breed also has a very good growth factor [1].In order to support the self-sufficient of beef meat, feedloters who imported BX from Australia have to conduct breeding program as part of the Ministry of Agriculture decree no. 02/PERMENTAN/PK.440/2/2017 related to the import of ruminants into Indonesia. BX Cattle is often crossed with other breeds through artificial insemination programs, in order to provide calving ease and to produce high-weight cattle.Beside the target to produce good body weight and average daily gain (ADG) of the calf from crossbreeding, the feedloters have also to manage the breeding efficiently by taking care of the reproductive disorder that might be affected the production.  Good reproductive efficiency will have a positive impact on increasing livestock production [2]. In various breeding programs, there are many factors that can affect the reproductive efficiency of cattle, such as reproductive disorders. Reproductive disorders can be a major economic problem in a farm. These reproductive health problems can be the bottleneck in the production process and productivity in the livestock sector [3].Therefore, this study will discuss the production performance in various cross-breed cattle and reproductive disorders in a feedlot breeding program.