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PAT-6 BRAIN HISTOPATHOLOGY OF CYNOMOLGUS MONKEY (MACACAFASCICULARIS) WITH MEMORY IMPAIRMENT INDICATED BY ALZHEIMER TYPE NEURODEGENERATIVE DISEASE Kusumaputri, Vinka Aftinata; Darusman, Huda Shalahudin; Agungpriyono, Dewi Ratih
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

The study is about the degenerative lesion found in nine cynomolgus monkeys that showed decline cognitive function during theirold age life. In previous studies, the brain of these cynomolgus monkeys has been tested positive for amyloid deposits by using the ELISA test.
ULTRASTRUCTURAL AND IMMUNOHISTOCHEMICAL STUDIES OF TRANSPLANTED CANINE LUNG CARCINOMA CELL TO SEVERE COMBINED IMMUNODEFICIENCY MICE (STUDI ULTRASTRUKTUR DAN IMUNOHISTOKIMIA TRANSPLANTASI SEL KANKER PARU-PARU ANJING PADA MENCIT SEVERE COMBINED IMMUNODEFF Sari, Dwi Kesuma; Priosoeryanto, Bambang Pontjo; Agungpriyono, Dewi Ratih; Yamaguchi, Ryoji; Uchida, Kazuyuki; Tateyama, Susumu
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Primary lung cancers, or tumors originating in the lung, are relatively uncommon in dogs. The objectiveof this study was to describe the canine lung carcinoma that serially transplanted into severe combinedimmunodeficiency (SCID) mice, in order to established cell line from this tumor cell.  Morphology andcharacteristic of this canine lung carcinoma in SCID mice by histopathological and ultrastructuralexaminations with metastatic lesion in lung were also examined.  Histopathologically, the tumor masswere consisted of cuboidal to columnar cells with papillary pattern, uniform in size, the nuclei were oftenvariable in size, and some cells have vacuole on their cytoplasms.  Glandular forms were predominant withlobulated pattern, ductal pattern with papillary injected into tube-like structure were also encountered.Mitotic figures commonly found with inflammatory reaction were sometimes present in the interstitiumand lumen gland.  Ultrastructural analysis of the tumor cells showed round to oval cells with one or moreprominent nucleoli.  The cells possessed numerous mitochondria, smooth endoplasmic reticulum, andindividual cells which were interconnected via desmosomes.  Tonofilament characterize by long cytoplasmicmaterial was encountered.  Positive reaction of the round to oval tumor cells to anti keratin antibodyconfirmed that their epithelial cell nature.  Lung metastatic lesions were found in SCID mice aftertransplantation and this phenomenon indicated that canine lung carcinoma is tumorigenic to SCID mice.
Liver and kidney histopathological test of mice received Pauh Kijang (Irvingia malayana Oliv ex A. Benn) extract ., Praptiwi; Agungpriyono, Dewi Ratih; Rahayu, Esti
INDONESIAN JOURNAL OF PHARMACY Vol 19 No 4, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.625 KB) | DOI: 10.14499/indonesianjpharm0iss0pp172-177

Abstract

The background of this study was to determine the safety of pauh kijang extract which is orally given to mice while the aim of the study was to evaluate the liver and kidney histopathological changes after receiving various dose of the extract mango (Irvingia malayana Oliv. Exe Benth.) ethanol extract. There were five group of treatments in this study : mice received extract with the concentration of (I) 1000mg /kg BW, (II)100 mg/kg BW, (III) 10 mg/kg BW, (IV) 1 mg/kg BW, and (V) received carboxy methyl cellulose-sodium (CMC-Na) as negative control. Each treatment consisted of five mice. After seven days of treatment, mice were sacrified. The liver and kidney were sampled and processed to prepare histopathology slides. The histopathology parameters were examined by counting the degeneration and necrotic cells of hepatocytes and renal epithelial tubule. The lesion of glomerulus such as atrophy and protein sedimentation were also observed. The result also showed that extract concentration of > 10 mg/kg BW result in damaging liver cells significantly, while kidney cell damage occurred significantly in the exposure of extract > 1 mg/kg BW.Key words : Irvingia malayana, liver, kidney
PENGOPTIMALAN KINERJA MOTORIK PADA PENUAAN FISIOLOGIS DAN PENUAAN AKIBAT STRES OKSIDATIF DENGAN ALANIN-GLUTAMIN DIPEPTIDA DAN HUBUNGANNYA DENGAN PERBAIKAN FUNGSI HIPOKAMPUS s, Sunarno; Manalu, Wasmen; Nastiti, Kusumorini; Agungpriyono, Dewi Ratih
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i1.354

Abstract

Penelitian ini bertujuan membuktikan pengaruh alanin-glutamin dipeptida 7% terhadap perbaikan fungsi hipokampus melalui pengamatan beberapa parameter kinerja motorik seperti jarak tempuh (JT), waktu stereotipe (WT), waktu ambulatori (WA), dan waktu istirahat (WI). Penelitian ini dilakukan dengan memberikan alanin-glutamin dipeptida 7% selama 12 hari pada tikus umur 12 dan 24 bulan yang mengalami penuaan fisiologis dan penuaan akibat stres oksidatif. Pengamatan terhadap semua parameter kinerja motorik dilakukan dengan alat Opto-Varimex yang dikoneksikan dengan software Autotract. Hasil penelitian menunjukkan alanin-glutamin dipeptida 7% berpengaruh terhadap perbaikan kinerja motorik pada kondisi penuaan fisiologis dan penuaan akibat stres oksidatif.
PATOGENESITAS VIRUS GUMBORO ISOLAT LOKAL PADA AYAM PEDAGING Wahyuwardani, Sutiastuti; Agungpriyono, Dewi Ratih; Parede, Lies; Manalu, Wasmen
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Study of the pathogenicity of gumboro virus (very virulent Infectious Bursal Disease virus, vvIBDV) oflocal isolates was performed in broilers. The chickens were grouped into four: i) infected with vvIBDV; ii)vaccinated with commercial vaccine + challenge with vvIBDV; iii) vaccinated using locally produced vaccine+ challenged with vvIBDV; and iv) control group (unvaccinated animals). Pathogenecity was analyzedbased on the sequence of infection; the distribution and degree of gross pathology and histopathologylesions on bursa Fabricius, spleen and thymus in relation to the presence of the antigen (IBDV Ag) whichwas detected using immunohistochemistry technique. During the acute phase (1-3 days following theexperimental infection) hyperemia and exudation on the bursa Fabricius were observed in the infectedgroup and the two vaccinated-challenged groups. Similarly, hyperemia was also observed on the spleenand thymus. Whilst during the chronic stages (at 7 and 14 days following the experimental infection) thebursa showed atrophy. Upon histological examination, the bursa showed interstitial edema, hemorrhage,infiltration of heterophyl cells and fibroblast hyperplasia. The bursa lymphoid follicles were depleted dueto cells necrosis and apoptosis. Plica epithelial layer was wrinkled and the Goblet cells metaplasia formedhyper plastic cysts. In the spleen and thymus severe reticulo endothelial cells proliferation was observedduring the acute stage and tended to decline during the chronic stage. During the acute and chronic stagescells containing IBDV Ag were detected in the bursa Fabricius, distributed within the mantle zone andgerminal center of lymphoid follicles in the two vaccinated-challenge groups. Whereas in the infectedgroup, the IBDV antigen were detected up to 7 days post the infection. Nevertheless, neither IBDV antigenwas detected in the spleen nor in the thymus in all the experimental groups. The results of this studyclearly explained the ferocity of vvIBDV of local isolates.
PENGARUH PEMBERIAN BUBUK EKSTRAK CU-TURUNAN KLOROFIL DAUN CINCAU (PREMNA OBLONGIJOLIA MERR.) TERHADAP PROFIL LIPID DARAH KELINCI Nurdin, Nurdin; Khomsan, Ali; Marliyati, Sri Anna; Anwar, Faisal; Kusharto, Clara Meliyanti; Agungpriyono, Dewi Ratih
Media Gizi dan Keluarga Vol. 32 No. 1 (2008): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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Abstract

ABSTRACT. The aim of this research was to produce cincau copper-derivative chlorophyll powder and to observe its phYSico-chemical properties and the effect on blood lipid profile of5 month male New Zealand White rabbits. The blood lipid profile observed were total cholesterol, triglyceride, HDL (high-denSity lipoprotein) cholesterol, and LDL (low-density lipoprotein) cholesterol. A two months treatment was conducted A randomblock design was applied with the following treatment on each experimental unit: PO (basal diet, negative control), PI (basal diet with cholesterol 0.1% (wlw) added, positive control), P2 (basal diet with cholesterol 0.1% (wlw) and 16.7 mglkg-body weight/d natural chlorophyll powder added), P3 (basal diet with cholesterol 0.1% (wlw) and 16.7 mglkgbody weight/d copper-derivative of chlorophyll powder added), P4 (basal diet withcholesterol 0.1% (wlw) and 50.1 mglkg-body weightld copper-derivative of chlorophyll powder added}, P5 (basal diet with cholesterol 0.1% (wlw), and 16.7 mglkg-body weight/dcommercial chlorophyll powder added The results of study showed that cincau leaves chlorophyll extract solution with Cu1+ 100 mglL level produce the cincau copper-derivate chlorophyll powder with highest pH, solubility, and greenness compared to other copper levels. Feeding with copper-derivative of chlorophyll cincau powder (P3) most likely decreased total cholesterol, triglycerides, LDL cholesterol increased the HDL cholesterol,and decreased atherosclerosis risk after 2 month as compare to feeding contain of natural chlorophyll (P2). Keywords: Premna oblongifolia Merr., copper-derivate chlorophyll powder, totalcholesterol, triglyceride. HDL cholesterol, LDL cholesterol
KOMUNITAS MAKROZOOBENTOS DAN AKUMULASI KROMIUM DI SUNGAI CIMANUK LAMA, JAWA BARAT Gitarama, Arbi Mei; Krisanti, Majariana; Agungpriyono, Dewi Ratih
Jurnal Ilmu Pertanian Indonesia Vol. 21 No. 1 (2016): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (601.419 KB) | DOI: 10.18343/jipi.21.1.48

Abstract

The residue of human activities and batik industrial waste water surrounding the river will be able to increase the accumulation of chromium and to disrupt macrozoobenthic communities in the river. The aims of this study was to assess the impact of human activities surrounding the river to the macrozoobenthic communities and the accumulation of chromium in Cimanuk Lama River, Indramayu District, West Java. The study has been conducted from April?May 2015 based on three times sampling at three different sampling stations. The result of this study shows that the chromium accumulated in the waters of all station still meet the standard quality ranging from 0,010-0,016 mg/l, but only station 1 fulfills the standard quality for chromium accumulated in the river sediment with the range of all stations was about 11,72-46,63 mg/kg. The results also show that the community index analysis describes the change of macrozoobenthic community at all stations caused by environmental pressure, especially at the station 2 which is indicated by the highest score of Family Biotic Index. The accumulation of the chromium in the waters and the change of macrozoobenthic community structure are mostly influenced by the use of the Cimanuk Lama river long for agricultural and fisheries activities, and also batik home industry.
DETEKSI DINI PENYAKIT TUMOR SEL DARAH MYELOSIT LEUKOSIS MELALUI PEMERIKSAAN ULAS DARAH Agungpriyono, Dewi Ratih; Huminto, Hernomoadi; Estuningsih, Sri; Satyaningtijas, Aryani Sismin
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Myelocytes blood cell tumor in chicken is a disease caused by retrovirus, avian leukosis virus subgroup J (ALV-J). The virus has the same group as human retrovirus (HIV) which caused AIDS, but the avian type possesses oncogenic properties, that could induce cell transformation and tumor formation. ALV-J stimulates the bone marrow's myelocytes and transforms them into tumor cell myelocytoma. The tumor cells then metastasis through the circulatory system causing myeloid leukosis and tumor cells accumulation in various internal organs or myelocytomatosis. This study was done on the base of the leukosis behavior of the tumor. The finding of the metastasis tumor cell in the blood smear examination is thought could be use as the diagnostic clue of the disease. Blood smear from sick chickens are collected and stained with some chemical staining substance such as may grunwald-giemsa, hematoxyllin eosin, periodic acid Schiff, congo red, toluidine blue, and sudan black B. Cytochemistry character of the blood cells was observed using light microscope. The result showed that myelocytes granules were best observed using hematoxyllin eosin, periodic acid Schiff, congo red and toluidine blue while may grunwald-giemsa, and sudan black B could not differentiate the granules. By this method, the field veterinarian will able to screen the suspected chicken flock for myeloid leukosis earlier than the occurrence of tumor formation.
GROWTH AND EXTRACELLULER POLYSACCARIDE PRODUCTION OF PORPHYRIDIUM CRUENTUM IN VARIOUS PHOTOPERIOD Prasetyo, Himawan; Setyaningsih, Iriani; Agungpriyono, Dewi Ratih
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 18 No. 2 (2015): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (777.701 KB) | DOI: 10.17844/jphpi.v18i2.12882

Abstract

Porphyridium cruentum is a kind of red microalgae (Rhodophyta) that can grow and producepolysaccharides. Polysaccharides are produced on the cell, then released and accumulated byextracellular into medias. This study aimed to determine the optimum growth and productionof extracellular polysaccharides (EPs) from P. cruentum in various photoperiods. The cultivationwas done with the photoperiods at 24:0, 18:6, 12:12 and 06:18 h (light:dark) during 40 days usingmodified medium F/2. The growth is determined by measuring optical density (OD) and driedbiomasses, the productions of EPs are determined by dried weight polysaccharides as a resultof precipitation. The results showed that OD at photoperiod 24:0 has the highest value 0.876 ±0.0645 unit of absorbance, biomass concentrations are highest in the photoperiod 24:0 and 12:12respectively 716.12 ± 123 mg-l and 696.5 ± 74.5 mg-l, the highest production of EPs on thephotoperiod 12:12 is 1.310 ± 130.26 mg-l. 12:12 hours (light: dark) photoperiod is optimally usedin P. cruentum cultivations with high biomass and extracellular polysaccharide results.
Pengaruh Pemberian Ekstrak Kulit Batang Ailanthus altissima terhadap Perubahan Sel Hati dan Ginjal Mencit Praptiwi, Praptiwi; Kuncari, Emma Sri; Agungpriyono, Dewi Ratih; Chairunnisa, Chairunnisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.777 KB) | DOI: 10.24002/biota.v15i1.2654

Abstract

The aim of this study was to determine the toxicopathological effect of Ailanthus altissima extract in liver and kidney of the mouse. The treatments in this study were: (1) negative control (carboxy methocellulose), extracts with concentration of (2) 1 mg/kg BW, (3) 10 mg/kg BW, (4) 100 mg/kg BW, and (5) 1000 mg/kg BW. Every treatment consisted of 5 mice. After receiving A.altissima extract for seven day consecutively, the mice were then euthanized with overdose of ether. The liver and kidney were taken and then fixed within 10% solution of Buffer Neutral Formalin (BNF). Histopathology slides of liver and kidney were processed and stained with Hematoxylline Eosin. The cell degeneration and cell death were observed with video photo microscope. The lesion of cell death and degeneration of hepatocytes and epithelium of kidney proximal tubule were counted. The data were analyzed statistically with ANOVA test continued with Duncan test. The result showed that cell necrosis of hepatocytes was not significantly different (P>0.05) compared to control treatment, while cell necrosis was increasing in the treatment of ? 10 mg/kg BW in tubuli of the kidney.