Articles

Found 39 Documents
Search

IMMOBILIZATION OF GLUCOSE OXIDASE ENZYME FROM PENICILLIUM SP-3 LOCAL STRAIN Ahmad, Ahyar; Syaiful, Andi; Firman AP, Firman AP; Patong, Abdul Rauf
Indonesian Journal of Chemistry Vol 7, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21721

Abstract

Immobilization of glucose oxidase (GOD) enzyme from Penicillium sp-3 local strain has been carried out using ionotropically entrapping method in  Ca-alginate membrane coupled with Na-polyacrilate. The entrapment of the enzyme in diffusion membrane occur spontaneously by cross-linking between Na-alginate/Na-polyacrilate and CaCl2. The GOD enzyme immobilizedby addition of 1 % Na-polyacrilate has the highest encapsulation efficiency, that is 87.13 % with the smallest percentage of diffusion, i.e. 23.37% and the relative activity of 50%. The GOD immobilized enzyme had good stability at the pH range 4 - 7 during 30 minutes of storage and was stable at a temperature of 20 oC. The activity of the GOD enzyme after being utilized continuously for 5 times only decrease up to 47,06 % compared to that in the initial utilization.
PROFILE OF AMINO ACIDS OF FOREST HONEYS FROM MALLAWA SOUTH SULAWESI Mahmud, Mahdalia; Noor, Alfian; Ahmad, Ahyar
Marina Chimica Acta Vol 19, No 1 (2018): Volume 19, No. 1 (2018)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271 KB) | DOI: 10.20956/mca.v19i1.4210

Abstract

Mallawa is one of the areas that has production forests as the second largest honey producer in Maros. The aim of this research determined the profile of amino acids in honey.  Amino acids of two forest honeys were analyzed using ultra performance liquid chromatography (UPLC). The main amino acids detected honeys were glutamic acid (1060.66 mg/kg, 928.85 mg/kg), histidine (700.37 mg/kg, 478.02 mg/kg), and arginin (683.11 mg/kg, 665.08 mg/kg), but tyrosine and serine not detected. Some other amino acids, including threonine, leucine, valine, phenylalanine and proline, displayed similar  values contents of 239.36 mg/kg, 268.15 mg/kg; 225.81 mg/kg, 293.44 mg/kg; 170.12 mg/kg, 226.03 mg/kg; 174.74 mg/kg, 288.11 mg/kg; and 161.58 mg/kg, 257.44 mg/kg, respectively.
PLANKTON ABUNDANCE BETWEEN DRY AND RAINY SEASON IN TALLO FRESHWATER AND PANGKEP RIVER Armus, Rakhmad; Noor, Alfian; Ahmad, Ahyar; Lukman, Muhammad
Marina Chimica Acta Vol 20, No 2 (2019): Volume 20, No. 2 (2019)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.933 KB) | DOI: 10.20956/mca.v20i2.9271

Abstract

Plankton abundance is influenced by seasons, sea surface currents (SSC), nutrients and surface temperature (SST). Is a marine organism has a function to stabilize the surface temperature of the earth by regulating the concentration of Dimetyl sulfide (DMS) due to exposure to direct sunlight. There is the ability of the ocean to regulate warming and to regulate the distribution of water vapor in the atmosphere which is controlled by sea surface temperature. Sampling using the transeck system in September and January represents the rainy season and the dry season which is closest to the Tallo river estuary and the Pangkep river estuary represents the estuary region, towards the coast of the spermonde (Litoral) islands and far from the coast (offshore) from freshwater sources, but the relationship of nutrient entry into estuary areas by sea surface currents (SSC) is still poorly known. The dominant aspect of phytoplankton is determined by the amount of nutrients flowing (runoff) from land to estuary to spermonde waters
EFFECT OF HEATING AND STORAGE TIME LEVELS 5- (HYDROXY METHYL) FURAN-2- KARBALDEHIDA (HMF) IN HONEY ORIGIN MALLAWA Suhaela, Suhaela; Noor, Alfian; Ahmad, Ahyar
Marina Chimica Acta Vol 17, No 2 (2016): Volume 17 No. 2 (2016)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.839 KB) | DOI: 10.20956/mca.v17i2.1124

Abstract

A research has been on determining the origin of forest honey quality Mallawa Maros based on the levels of 5-(hydroxymethyl)furfural-2-karbaldehida (HMF)is quantitatively based on the variation of heating temperature and duration of storage with White spectrophotometric method. The results showed that the levels of forest honey HMF origin Mallawa on heating temperature 35°C,90°C and 110°C respectively is 42.2023 mg/Kg, 46.0274 mg/Kg and 62.2520 mg/Kg. While the storage duration for 3,4, and 5 months had higher levels of HMF each 27.4309 mg/Kg, 42.5158 mg/Kg and 48.9405 mg/Kg. Heating temperature and storage time a big influence on levels of HMF and show a strong relationship to the water content, pH and free acid, reducing sugar content and diastase enzyme activity. Based on the research results, levels of HMF at the heating temperature of 110oC has passed the standards set by the SNI (up to 50 mg/Kg). Whereas the standard set by IHC for honey originating from thetropics (80 mg/ Kg)
PURIFICATION, ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE FRACTION FROM RED ALGAE GRACILARIA VERRUCOSA Muawanah, Muawanah; Ahmad, Ahyar; Natsir, Hasnah
Marina Chimica Acta Vol 18, No 2 (2017): Volume 18 No. 2 (2017)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/mca.v18i2.2688

Abstract

 it contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity of polysaccharide extract and fraction fromred algae Gracilariaverrucosa. The study was carried out by isolating the polysaccharide extract using aqueous 100 o C and methanol-ethanol precipitation .Purification of polysaccharide done by Column Chromatography DEAE-Cellulose and analysis polysaccharide concentration was using phenol-sulfuric acid. Antioxidant activity of crude extract and polysaccharide fraction was examined using DPPH method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results reveals that the highest polysaccharide concentration was fraction 7 with value of 0,267 mg/mL, and  the crude extract and fraction  4 have strongest antioxidant activity with IC50 values of 51,22 and 62,25 ppm, respectively. The result of BSLT assay showed that the crude extract and fraction 6 has a highest toxicity with LC50 values of 47,73and 58,48 ppm, therefore it is classified as toxic. The polysaccharide of crude extract and fraction 7 has a potential to be developed as an alternative antioxidant and antitumor agent. Identification with FT-IR shows the characteristic bands of agarocolloid.
ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE EXTRACT FROM RED ALGAE EUCHEUMA COTTONII AND EUCHEUMA SPINOSUM Muawanah, Muawanah; Ahmad, Ahyar; Natsir, Hasnah
Marina Chimica Acta Vol 17, No 2 (2016): Volume 17 No. 2 (2016)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.837 KB) | DOI: 10.20956/mca.v17i2.1123

Abstract

Red algae Eucheuma cottonii and Eucheuma spinosum which one alternative source of antioxidant and anticancer because contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity polysaccharide extract from red algae Eucheuma cottonii and Eucheuma spinosum . The study was carried out by isolating the polysaccharide extract using water and methanol-ethanol precipitation. Antioxidant activity of crude extract was examined using 1,1-Difenil-2-pikrilhidrazil (DPPH) method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results showed that the crude extract polysaccharide for Eucheuma cottonii and Eucheuma spinosum have strong antioxidant activity with IC50 value of 72,49 ppm and 75,98 ppm. The result of BSLT assay showed that the crude extract polysaccharide has a highest toxicity with LC50 value of 165,88 ppm and 337,21 ppm there are classified as toxic. The crude extract polysaccharide Eucheuma cottonii and Eucheuma spinosum has a potential to be developed as an alternative antioxidant and anticancer agent.
ANALYSIS OF DIMETHYLSULFIDE AS BIOGEOCHEMICAL CYCLES SULFUR ON REGIONAL ESTUARY SPERMONDE Armus, Rakhmad; Noor, Alfian; Ahmad, Ahyar; Lukman, Muhammad
Marina Chimica Acta Vol 19, No 1 (2018): Volume 19, No. 1 (2018)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.417 KB) | DOI: 10.20956/mca.v19i1.4208

Abstract

We developed a more accurate method of analysis for low-concentration volatile compounds used for the determination of free dimethylsulfide (DMS) in marine phytoplankton by using solid phase (SPME) gas chromatography - spectrometry Mass of GC-MS and head space method. DMSP, as a product of bacteria degradation has been scientifically recognized to be affected by climate change due to increased in sea surface temperature, and form DMS compunds.. The present study is to determine the DMSP concentration and degradation pathway in the oligotrophic region of Spermonde waters. The spatial and temporal correlation between DMSP degrading bacterial codes and biological potential controls for DMS formation in the open seas was examined using a statistical factorial analysis of variance and regression against the spatial (or distance) gradients of the Spermonde coastal waters, the concentration of chlorophyll-a, and diatom community structures. Seawater chemical properties, chlorophyll-a, and diatom samples were collected from several sites in the Tallo-Makassar river estuary, and the Pangkep river in September 2017 (transitional wet-to-dry-season). The result shows that the concentration of the Domination Index was higher in the transition season of 35.2 to 85.2 ?M than in other seasons. The abundance of diatoms during the transition season reached 16,534 plankter / mL.
TOXICITY ASSESSMENT ON NINE SPONGES SPECIES FROM SPERMONDE ARCHIPELAGO Sapar, Ajuk; Noor, Alfian; Soekamto, Nunuk Hariani; Ahmad, Ahyar; Hadi, Tri Aryono
Marina Chimica Acta Vol 16, No 2 (2015): Volume 16 No. 2 (2015)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/mca.v16i2.2542

Abstract

Toxicity assessment have been conducted on nine sponges species collected from Spermonde Archipelago namely Kapoposang Island, Samalona Island and Lanjukang Island. Toxicity assessment was using Brine Shrimp lethality Test (BST) method againts sponge extracts. Theresults test were expressed as 50% lethal concentration (LC50) to Artemia salina and correlated with prospecting extract potential as anticancer. All extracts obtained from smallscale extraction by methanol. Test results as follows: Acanthostrongylophora ingens (24.830 ?g/mL), Hytios erectus (46.140 ?g/mL), Stylissa massa (>1000 ?g/mL), Theonella sp (35.010 ?g/mL), Niphates olemda (87.500 ppm), Haliclona (Reniera) fascigera (78.160 ?g/mL), Petrosia (Petrosia) sp (3.008), Callyspongia (Euplacella) biru (0.515 ?g/mL). The test results showed that seven species are potential as anticancer with LC50 value ? 500 ppm and only Stylissa massa extracts was not potential as anticancer.
BIOACTIVITY OF PROTEIN FRACTION IN BROWN ALGAE, TURBINARIA DECURRENS, AS ANTIBACTERIAL AGENT BIOAKTIVITAS FRAKSI PROTEIN DALAM ALGA COKLAT, TURBINARIA DECURRENS, SEBAGAI AGEN ANTI BAKTERI Dali, Seniwati; Natsir, Hasnah; Usman, Hanapi; Ahmad, Ahyar
Marina Chimica Acta Vol 14, No 1 (2013): Volume 14 No. 1 (2013)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.944 KB) | DOI: 10.20956/mca.v14i1.2930

Abstract

Penelitian ini bermaksud mengeksplorasi dan mengkarakterisasi fraksi protein dari alga cokelat Turbinaria decurrens di Sulawesi Selatan. Senyawa protein bioaktif diperoleh melalui serangkaian proses ekstraksi, fraksionasi dengan kejenuhan amonium sulfat 0-20%, 20-40%, 40-60%, dan 60-80% yang dilanjutkan dengan proses dialisis lalu ditentukan kadar proteinnya dengan metode Lowry. Fraksi protein yang diperoleh diuji bioaktivitasnya terhadap bakteri Staphylococcus aureus dan Salmonella thypi dengan metode difusi agar pada medium MHA. Hasil penelitian menunjukkan bahwa kadar protein tertinggi ditemukan pada fraksi 0-20% yaitu sebesar 5,1 mg/mL. Hasil uji bioaktivitas antibakteri terbesar ditemukan pada fraksi protein 0-20% terhadap Staphylococcus aureus dan Salmonella thypi dengan zona daerah hambatan masing-masing sebesar 18,33 mm dan 13,30 mm yang bersifat bakteriostatik. Hasil penelitian awal ini akan dikembangkan pada cakupan penelitian yang lebih luas untuk mengkloning protein bioaktifnya dan uji pre-klinis dari protein antibakteri yang diperoleh.
SURFACE TEMPERATURE DISTRIBUTION ANALYSIS USING REMOTE SENSING SYSTEM IN SPERMONDE ESTUARY Armus, Rakhmad; Noor, Alfian; Ahmad, Ahyar; Lukman, Muhammad
Marina Chimica Acta Vol 19, No 2 (2018): Volume 19, No. 2 (2018)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.556 KB) | DOI: 10.20956/mca.v19i2.5388

Abstract

Research on the phenomenon of climate change on the region Makassar Strait waters that are part of the oceanographic activities that require observation of the sea surface temperature (SST), surface currents and ocean color imagery from satellite imagery. In this case, satellite remote sensing data using Aqua-Modis analyze both visually and raw-surface temperature parameter data in order to study a variety of other related phenomena that take place in the ocean. The existence of satellite imagery data for the observation of parameters and / or oceanographic phenomena will be more profitable in terms of time and cost and high accuracy. The purpose of this study was to review based on the description of the SST analysis using Aqua-Modis image data (non-commercial) and open source software in providing the information cepatdan applicable. Bodies Spermonde been considering the seas is quite unique because it is located right in the path of the current meeting of the Pacific Ocean through the sea of Sulawesi and spacious enough for the continuity of mesoscale oceanographic processes for coverage in the waters Spermonde in (islands) Indonesia