Luqman Qurata Aini
Jurusan Hama dan Penyakit Tanaman, Fakultas Pertanian, Universitas Brawijaya

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IDENTIFICATION OF BACTERIAL WILT AND LEAF BLIGHT DISEASE ON MAIZE (ZEA MAYS) FOUND IN KEDIRI, INDONESIA Aini, Luqman Qurata; Suryani, Lilis; Sugiharto, Arifin Noor; Abadi, Abdul Latief
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.245

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Recently, a new bacterial disease of maize (Zea mays) was observed in Kediri, East Java, Indonesia. Infected plants showed wilt symptoms occasionally accompanied by leaf blight. This study aims to characterize the causal agent of bacterial wilt and leaf blight of maize observed in Kediri. Gram-negative, facultative anaerobic bacteria were isolated from the diseased tissues. All bacterial strains (KD1A, KB2A, KD1, KD4, KB1) gave positive result both in the hypersensitive response and pathogen-nicity assays. However, only KD1 and KB1 strains could be re-isolated from the diseased tissues. Based on several physiological and biochemical assays, the bacteria resembled Pantoea agglomerans. Moreover, the strains showed negative result on PCR amplification using HRP1d and HRP3r, a primer pair specific for detection of P. stewartii subsp. stewartii. Analysis of 16S rRNA gene sequence of KD1 and KB1 showed highest homology at 88% and 90%, respectively to P. agglomerans strain DSM 3493 (NR 0419781).  The homology values were too low to conclude that the bacteria were similar to P. agglomerans. These results suggest that bacterial pathogens isolated from maize in Kediri were strains of Pantoea sp. Keywords: maize, Pantoea sp., wilt, leaf blight
KARAKTERISASI BAKTERI PENYEBAB PENYAKIT HAWAR DAUN EDAMAME DI JEMBER Masnilah, Rachmi; Abadi, Abd Latif; Astono, Tutung Hadi; Aini, Luqman Qurata
Berkala Ilmiah Pertanian Vol 1, No 1: AGUSTUS 2013
Publisher : Berkala Ilmiah Pertanian

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[ENGLISH] One among important diseases in Edamame soybean is bacterial bligh. The disease is relatively new and causes significant loss of soybean yield. This research was aim to know the characterize the pathogen of bacterial blight in Jember. Survey was one in several soybean field in Jember and the plant sample was culture on Kings B medium to grow the pathogen. Identification was done through pysiological and biochemistry assay. The result showed that the pathogen of bacterial blight disease on Edamame soybean di Jember (Panti, Sukorambi, Wirolegi, Ajung, and Sumbersari) was Pseudomonas syringae pv. glycinea with properties as Gram negative, produce fluorescent pigment on Kings B medium, grew on 20-40O C, pH range of 4.5-8.5, tolerant on 0.5-2% NaCl and also pathogenic and virulent on Edamame while reinoculated to Edamame. Keywords: Edamame, bacterial blight, soybean  [INDONESIAN] Salah satu penyakit penting pada kedelai khususnya edamame ialah hawar daun bakteri, penyakit ini merupakan penyakit yang relatif baru dan menyebabkan kerugian yang cukup signifikan. Penelitian ini bertujuan untuk mengetahui karakteristik bakteri penyebab penyakit hawar daun edamame di daerah Jember. Penelitian diawali dengan pengamatan gejala di lapangan di beberapa lokasi di daerah Jember. Bagian tanaman yang sakit dibawa ke laboratorium, selanjutnya dilakukan isolasi pada medium King B. Identifikasi dilakukan dengan serangkaian pengujian fisiologi dan biokimia. Berdasarkan pengujian yang telah dilakukan disimpulkan bahwa bakteri penyebab penyakit hawar daun edamame di Jember (Panti, Sukorambi, Wirolegi, Ajung, dan Sumbersari) ialah Pseudomonas syringae pv. glycinea yang bersifat gram negatif, mampu membentuk pigmen fluoresen pada medium king B, mampu tumbuh baik pada kisaran suhu 20-400 C, mempunyai kisaran pH 4,5-8,5, toleran pada kandungan NaCl 0,5-2%, serta bersifat patogenik dan virulen pada tanaman edamame. Keywords: Edamame; Hawar Daun; Kedelai How to citate: Masnilah R, AL Abadi, TH Astono, LQ Aini. 2013. Karakterisasi bakteri penyebab penyakit hawar daun edamame di Jember. Berkala Ilmiah Pertanian 1(1): 10-14.
APLIKASI AGENS HAYATI DAN BAHAN NABATI SEBAGAI PENGENDALIAN LAYU BAKTERI (Ralstonia solanacearum) PADA BUDIDAYA TANAMAN TOMAT Maharina, Khoirun Enisa; Aini, Luqman Qurata; Wardiyati, Tatiek
Jurnal Produksi Tanaman Vol 1, No 6 (2013)
Publisher : Jurusan Produksi Tanaman Fakultas Pertanian Universitas Brawijaya

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Abstract

Buah tomat merupakan salah satu produk hortikultura yang dibutuhkan untuk konsumsi rumah tangga. Berdasarkan hasil laporan BPS (2013), terjadi penurunan produksi buah tomat pada tahun 2012 sebesar 6,96 %. Kegiatan pengendalian terpadu (PHT) menggunakan agens hayati dan pestisida nabati dapat menjadi suatu upaya meningkatkan produksi khususnya di dataran menengah. Tujuan penelitian ini ialah untuk mengetahui potensi agens hayati dan bahan nabati sebagai pestisida alternatif dalam menekan populasi bakteri Ralstonia solanacearum, serta pengaruhnya terhadap pertumbuhan dan perkembangan tanaman tomat di dataran menengah, serta untuk mengetahui pengaruh interval aplikasi pestisida terhadap perkembangan populasi Ralstonia solanacearum di dalam tanah. Hasil penelitian menunjukkan bahwa aplikasi pestisida alternatif dan interval aplikasi berpengaruh terhadap parameter tinggi tanaman, jumlah daun, jumlah bunga, jumlah buah, bobot buah, persentase tanaman sakit, dan populasi Ralstonia solanacearum di dalam tanah. Aplikasi agens hayati berpotensi menekan serangan penyakit layu bakteri dan populasi Ralstonia solanacearum. Agens hayati juga dapat meningkatkan produksi buah tomat. Pestisida nabati ekstrak daun sirih dapat meningkatkan pertumbuhan tanaman dalam hal tinggi tanaman dan jumlah daun. Kata kunci: Tomat, Layu bakteri, Agen hayati, Daun sirih, Kubis
The effect of PGPR (Plant Growth Promoting Rhizobacteria) Pseudomonas fluorescens and Bacillus subtilis On Leaf Mustard Plant (Brassica juncea L.) Infected by TuMV (Turnip Mosaic Virus) Diyansah, Bogi; Aini, Luqman Qurata; Hadiastono, Tutung
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

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Abstract

AbstractOne problem that causing leaf mustard yield loss is the infection of Turnip Mosaic Virus (TuMV). Thevirus causes mild mosaic leaf with vein clearing, blister, malformation and stunting. The use of Plant Growth Promoting Rhizobacteria (PGPR) such as Pseudomonas fluorescens and Bacillus subtilis is oneeffort that could be used to solve the problem. Through the mechanism of induced resistance, thesebacteria can elicit the defense signal in plant for the defence against pathogens. In this study the use of Pseudomonas fluorescens and Bacillus subtilis was performed to test their benefit on leaf mustard planthealth against TuMV infection. This study was conducted with a randomized block design (RBD) byusing 8 treatments and 4 replications. On the experiment of the effect of PGPR on the root length ofleaf mustard plants, the design used was a completely randomized design (CRD) with 4 treatments and4 replications. Leaf mustard plants inoculated with Pseudomonas fluorescens and Bacillus subtilis hadlonger roots than those without the inoculation of Pseudomonas fluorescens and Bacillus subtilis. Inaddition, Pseudomonas fluorescens and Bacillus subtilis was also able to reduce the incubation periodand disease intensity of TuMV on the leaf mustard plant. The activity of catalase enzyme and phenolcontent was elevated in the leaf of leaf mustard plant inoculated with PGPR. The results suggested thatcatalase and phenol production probably play a role in plant defense of leaf mustard against theinfection of TuMV.Key word : Mustard, Turnip Mosaic Virus (TuMV), PGPR
Efektifitas Pestisida Biologis Bacillus Cereus dan Bacillus Megaterium. sebagai Pengendali Spodoptera litura Fabr (Lepidoptera: Noctuidae) Agastya, I Made Indra; Afandhi, Aminudin; Aini, Luqman Qurata
Jurnal Penelitian Pertanian Terapan Vol 17, No 2 (2017)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.936 KB) | DOI: 10.25181/jppt.v17i2.294

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This research was conducted in vitro in the laboratory. Studies conducted in the Laboratory of Bacteriology Department of Plant Pests and Diseases of the Faculty of Agriculture, University of Brawijaya, on the effectiveness of the bacteria Bacillus cereus and Bacillus megaterium as biological pesticides controlling Spodoptera litura. The objective of this study was to determine the effectiveness of the bacteria Bacillus sp as biological control. Effectiveness pesticide was measured by testing the incubation period and mortality in larvae of S. litura instar 3. This study used a completely randomized design (CRD), conducted observations every 6 hours until the larvae dead. The results showed that the percentage of mortality of S. litura reached 94.66% compared to the control, while the incubation time of the bacteria B. cereus cause disease until 29.84 hours. B. cereus and B. megaterium have the ability to incubate third instar larvae of S. litura up to 29.84 hours and caused the death of larvae up to 94.66%. Keywords: Biological pesticides, Bacillus cereus, Bacillus megaterium, entomopatogen, Spodoptera litura
MICROBIAL COMMUNITY ASSOCIATED WITH AMBROSIA BEETLE, Euplatypus parallelus ON SONOKEMBANG, Pterocarpus indicus IN MALANG Tarno, Hagus; Septia, Erfan Dani; Aini, Luqman Qurata
AGRIVITA, Journal of Agricultural Science Vol 38, No 3 (2016): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i3.628

Abstract

Recently, most of sonokembang, Pterocarpus indicus trees are dying in Malang.  In 2012, the death rate of trees reached ca. 11%. In addition, death of trees spread to other regencies in East Java. Euplatypus parallelus is a specific species of ambrosia beetles that were the causal agents to the dying and wilting of sonokembang trees in Malang. Wilting is caused mainly by the pathogenic fungi carried by ambrosia beetles. To confirm the microbial communities related to E. parallelus that attack sonokembang, E. parallelus and some attacked trees were collected in Malang city. Isolation and identification of these species were conducted at the Laboratory of Mycology, Faculty of Agriculture, University of Brawijaya and Laboratory of Molecular Biology, Islamic State University, Malang. Results showed that there were nine microbes including five genera of fungi, two genera of yeasts and one genus of bacterium were identified. The microbial communities that were found namely Aspergillus spp., Penicillium spp., Trichoderma spp., Fusarium spp., Acremonium spp., Gliocladium spp. (fungi), Streptomyces spp. (bacteria), Saccharomyces spp., and Candida spp. (yeast).    
EKSPLORASI KAPANG ANTAGONIS DAN KAPANG PATOGEN TANAMAN APEL DI LAHAN PERKEBUNAN APEL PONCOKUSUMO Pradana, Galuh Setyanto; Ardiyati, Tri; Aini, Luqman Qurata
Biotropika: Journal of Tropical Biology Vol 1, No 1 (2013)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

Poncokusumo merupakan daerah pertanian di Kabupaten Malang yang memiliki komoditas utama yaitu apel. Serangan kapang patogen menyebabkan apel menjadi rusak dan busuk. Selama ini, pengendalian kapang patogen hanya dilakukan dengan menggunakan pestisida dan mampu menyebabkan terjadinya resistensi kapang patogen. Penelitian ini bertujuan mengetahui spesies kapang antagonis dan kapang patogen yang terdeteksi serta mengetahui tingkat dan mekanisme penghambatan kapang antagonis terhadap pertumbuhan kapang patogen dari lahan perkebunan apel Poncokusumo. Isolasi kapang antagonis dilakukan menggunakan sampel tanah top soil 10 cm, sedangkan isolasi kapang patogen berasal dari sampel organ tanaman yang terserang penyakit dengan melakukan sterilisasi menggunakan larutan NaOCl 5 %. Isolat kapang antagonis diuji tingkat penghambatannya terhadap kapang patogen tanaman apel dengan menggunakan metode dual culture dan slide culture. Persentase penghambatan masing-masing kapang antagonis dianalisis secara statistik menggunakan one-way ANOVA. Hasil eksplorasi didapatkan tiga genus kapang patogen yaitu Venturia sp., Colletotrichum sp., dan Monilia sp., sedangkan kapang antagonis yang didapat antara lain Trichoderma sp.(I), Trichoderma sp.(II), Trichoderma sp.(III), Aspergillus sp.(I), dan Aspergillus sp.(II). Penghambatan terbaik ditunjukkan kapang antagonis Trichoderma sp.(I) yaitu menghambat Venturia sp. sebesar 50,51%, Colletotrichum sp. sebesar 73,30%, dan Monilia sp. sebesar 66,97%. Hasil pengamatan mikroskopis diketahui bahwa mekanisme penghambatan kapang antagonis terhadap kapang patogen menggunakan metode slide culture diketahui bahwa isolat Genus Trichoderma yaitu kompetisi dan parasitisme, sedangkan isolat Genus Aspergillus dengan antibiosis. Kata kunci: antagonis, eksplorasi, patogen, penghambatan.
Leaf Extract of Mirabilis jalapa L. Induced Defense of Tomato Plant (Lycopersicum esculentum Mill.) Against Cucumber Mosaic Virus (CMV) Infection Kusumawati, Dian Eka; Hadiastono, Tutung; Aini, Luqman Qurata
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

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AbstractInfection of Cucumber Mosaic Virus (CMV) could reduce the tomato production up to 50%depending on the age of the plant during infection and tomato varieties. Until now the controlefforts against CMV infection have been made, but no consistent effective method was available.Recently, new approach by increasing the plant health to defense against plant disease wasincreased. It can be stimulated by inoculation of microbes associated with roots or by application of plant extract such as Mirabilis jalapa. In this study, the effect of M. jalapa leaf extract in differentconcentration to develop control against CMV infection in tomato plants was conducted. The results showed that the application of M. jalapa leaf extract could inhibit the development of CMV diseasein tomato plants. The higher the concentration of M. jalapa leaf extract applied on tomato plant, thelower disease incidence as well as the longer incubation period of CMV disease in tomato plants.The applications of M. jalapa leaf extract elevated the activity of peroxidase and catalase enzymes.Thus, the inhibition of CMV disease development in tomato plant by application of M. jalapa leafextract may related to the induction of peroxidase and catalase activity in tomato plants.Keywords: M. jalapa, CMV, plant defense, leaf extract, peroxidase, catalase
The Application of Beauveria bassiana and Lufenuron Could Reduce the Reproduction of Fruit Fly (Bactrocera carambolae) (Drew dan Hancock) (DIPTERA: TEPHRITIDAE) Hadi, Mochammad Syamsul; Himawan, Toto; Aini, Luqman Qurata
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

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Abstract

AbstractThis study was conducted to examine the effect of Beauveria bassiana and insect growth regulator lufenuron on the fecundity and reproductivity decline of fruit fly B. carambolae adults. The study was conducted at the Laboratory of Entomology, Department of Plant Pests and Diseases, Facultyof Agriculture, University of Brawijaya from March 2012 until December 2012. The density of B. bassiana spores used in this study was 108 spores/ml and the lufenuron concentrations used are 0.5, 1, and 1.5 ml/l. The results showed that the application of B. bassiana spores at density of 108 spores/ml combined with lufenuron at the concentration of 1.5 ml/l significantly reduced the fecundity, egg fertility and reproduction of B. carambolae up to 95.69%.Keywords: Beauveria bassiana, Bactrocera carambolae, Lufenuron.
SCREENING OF RHIZOSPHERE BACTERIA FROM CLOVE (SYZYGIUM AROMATICUM) IN TIDORE ISLAND AS PLANT GROWTH PROMOTING RHIZOBACTERIA Ishak, Ismat; Ardyati, Tri; Aini, Luqman Qurata
The Journal of Experimental Life Science Vol 8, No 3 (2018)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1176.245 KB) | DOI: 10.21776/ub.jels.2018.008.03.04

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Tidore Island in North Maluku Province is one of the clove (Syzygium aromaticum) producing regions. Clove plant fertility is maintained even though it is not given organic and inorganic fertilizers, it also keeps the fertility of the soil and the environment as a whole. Therefore, this study aims to explore bacteria in the rhizosphere of the clove trees planted in Tidore island as biofertilizer agents of Indol-3-Acetic Acid (IAA) production, phosphate solubilization, and ammonium production. Bacteria were isolated from the rhizosphere of clove trees. Screening of the isolates was done according to qualitative and quantitative methods. Data collection of the qualitative method were based on medium color changes for each variable while that the quantitative method (i.e. the concentrations of produced IAA, solubilized phosphate, and produced ammonium) were obtained based on spectrophotometry. The results obtained 110 bacterial isolates. Nineteen bacteria were potential as biofertilizer agents, of which isolate R11, R8P, and A1N showed the highest in IAA production (8.71 ?gL-1), phosphate solubilization (8.37 ?gL-1), and ammonium production (11.71 ?gL-1), respectively. The three isolates respectively have similarities to the genus of Enterobacter, Burkholderia, and Stenotrophomonas.Keywords: Biofertilizer, Clove, Rhizobacteria, Rhizosphere.