EDUARDUS BIMO AKSONO
Tropical Disease Diagnostic Center (TDDC) – Institute of Tropical Disease, Universitas Airlangga.

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QUICK DIAGNOSIS OF JAPANESE ENCEPHALITIS FOR NEW DIAGNOSED EMERGING DISEASE USING PCR TECHNIQUE IN SURABAYA, INDONESIA Matondang, Muhammad Qushai Y.; Nasronudin, Nasronudin; Aksono, Eduardus Bimo; Lusida, Maria Inge; Nastri, Aldise Mareta; Fajar, Nur Syamsiatul; Jannah, Lilis Mundri
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.4 KB)

Abstract

Background: Japanese enchepalitis (JE) is a viral disease that considered as zoonotic disease, which transmitted through mosquito vectors that had JE virus. Mainly caused by the mosquito C. Tritaeniorhynchus (the most important vector is the mosquito Culex, which feeds on cattle in preference to human). JE virus disease can also cause disturbances in the central nervous system eg. brain, bone marrow, and meninges which has serious impact on public health. This disease has been reported from Japan, Korea, Taiwan, India, Myanmar, Thailand, Western Pacific and Southeast Asia to Indonesia. However, the incidence of this disease in Indonesia has not been well known in various animal species or humans. Aim: The purpose of this study is to develop rapid diagnostic examinations on patient diagnosed JE virus in Surabaya by using PCR (Polymerase Chain Reaction). Because, JE disease can lead to dead-end at the patient if not treated immediately. Method: The research methods, extraction method, PCR (1st RT-PCR and 2nd Nested PCR) are conducted using Japanese encephalitis PCR detection kit. Result: The results of the examination showed that 2 out of 17 people (11,765%) are positive with PCR bands 227 bp (basepair). This diagnostic technique to determine and to deal with early onset of the disease. Solutions for preventive actions can be started from the termination of the cycle vectors to vaccination measures. Conclusion: For his own medical factors given to reduce fever and swelling and reduce the pain.
QUICK DIAGNOSIS OF JAPANESE ENCEPHALITIS FOR NEW DIAGNOSED EMERGING DISEASE USING PCR TECHNIQUE IN SURABAYA, INDONESIA Matondang, Muhammad Qushai Y.; Nasronudin, Nasronudin; Aksono, Eduardus Bimo; Lusida, Maria Inge; Nastri, Aldise Mareta; Fajar, Nur Syamsiatul; Jannah, Lilis Mundri
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.4 KB) | DOI: 10.20473/ijtid.v4i4.232

Abstract

Background: Japanese enchepalitis (JE) is a viral disease that considered as zoonotic disease, which transmitted through mosquito vectors that had JE virus. Mainly caused by the mosquito C. Tritaeniorhynchus (the most important vector is the mosquito Culex, which feeds on cattle in preference to human). JE virus disease can also cause disturbances in the central nervous system eg. brain, bone marrow, and meninges which has serious impact on public health. This disease has been reported from Japan, Korea, Taiwan, India, Myanmar, Thailand, Western Pacific and Southeast Asia to Indonesia. However, the incidence of this disease in Indonesia has not been well known in various animal species or humans. Aim: The purpose of this study is to develop rapid diagnostic examinations on patient diagnosed JE virus in Surabaya by using PCR (Polymerase Chain Reaction). Because, JE disease can lead to dead-end at the patient if not treated immediately. Method: The research methods, extraction method, PCR (1st RT-PCR and 2nd Nested PCR) are conducted using Japanese encephalitis PCR detection kit. Result: The results of the examination showed that 2 out of 17 people (11,765%) are positive with PCR bands 227 bp (basepair). This diagnostic technique to determine and to deal with early onset of the disease. Solutions for preventive actions can be started from the termination of the cycle vectors to vaccination measures. Conclusion: For his own medical factors given to reduce fever and swelling and reduce the pain.
NO ASSOCIATION OF RECURRENT RESPIRATORY PAPILLOMATOSIS AGGRESSIVENESS AND HUMAN PAPILLOMAVIRUS TYPE 6 AND 11 Perdana, Rizka Fathoni; Herawati, Sri; Suroso, Bakti; Aksono, Eduardus Bimo
Indonesian Journal of Tropical and Infectious Disease VOL 6, NO 5 (2017)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.917 KB) | DOI: 10.20473/ijtid.v6i5.3705

Abstract

Recurrent Respiratory Papillomatosis (RRP) is the most common benign neoplasm of the larynx among children as a result of HPV infection mainly type 6 and 11. RRP is still considered as serious problem since papilloma in the airway can cause hoarseness and obstruction which later described as aggressive and non aggressive types. Patients underwent multiple surgeries to keep airway patency and in order to communicate vocally. Previous studies reported that HPV-11 was associated with a more aggressive disease course. This study aim is to determine the association of RRP aggressiveness with HPV-6 and 11. Papilloma specimens were taken from patients who underwent surgical treatment of RRP patients and subjected HPV typing. All 17 patients with completed data and epidemiologic questionaires were defined for their staging to disease severity. Informations in the database were analyzed to identity statistically significant relationship with HPV type and knowing the HPV type is to predict the disease course. 17 patients RRP (12 males and 5 females) with age at onset 3,15 + 2,28 years and age at diagnosis 3,81 + 2,51 years. Surgery was performed 2,71 ± 1,16 times per year and 9 patients treated more than 3 surgeries per year. 10 patients had distal papilloma and 11 patients had tracheotomy. Agrressive disease was show found in 14 patients among 17 patients. HPV-11 was detected in 10 patients, HPV-6 was detected in 7 patients. According to the statisitcal analysis (Chi square test), there is no relationship between HPV type and disease aggressiveness. HPV-6 and 11 are not the only cause that affect the aggressiveness of Recurrent Respiratory Papillomatosis.
Bakteri Legionella pneumophila Terdeteksi pada Air Kolam Renang di Kota Surabaya dengan Nested Polymerase Chain Reaction (LEGIONELLA PNEUMOPHILA BACTERIADETECTED IN SWIMMING POOL WATER OF SURABAYA BY USING NESTED POLYMERASE CHAIN REACTION) Aksono, Eduardus Bimo; Farahdiba, Ana Adelina; Hestianah, Eka Pramyrtha
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.466 KB) | DOI: 10.19087/jveteriner.2017.18.2.221

Abstract

Legionella pneumophila is a Gram-negative bacillus that causes nosocomial and community-acquired pneumonia. The aim of this research was to detect the presence of bacteria of L. pneumophila species in the swimming pools water of Surabaya city by using nested Polymerase Chain Reaction (PCR) assay of a specific gene for L. pneumophila (mip gene). This study used purposive sampling method. A total of 10 water samples were collected from five swimming pools consisting of 200 mL water for each swimming pool. The results showed that of 10 samples tested by nested PCR, one sample was positive for L. pneumophila, and nine samples were negative. L. pneumophila were found in pool water samples with a higher temperature (>30ºC).Serogrouping analysis of positive sample that L. pneumophila bacteria detected in the water sample of swimming pool in Surabaya was L. pneumophila serogroup 9 (98%) and serogroup 10 (98%). L. pneumophila detection of bacteria is expected to raise the awareness of physician and microbiologists about the transmission of L. pneumophila and will also be useful for controlling the agents. ABSTRAK Legionella pneumophila adalah bakteri Gram-negatif berbentuk batang yang dapat menyebabkan penyakit nosokomial dan pneumonia. Tujuan penelitian ini adalah untuk mendeteksi keberadaan bakteri L. pneumophila pada air kolam renang di Kota Surabaya dengan menggunakan nested Polymerase Chain Reaction (PCR) berbasis gen spesifik L. pneumophila (mip gene). Penelitian ini menggunakan metode purposive sampling. Sebanyak sepuluh sampel diambil dari lima kolam renang. Sampel diambil sebanyak 200 mL dari air kolam renang di setiap lokasi. Hasil dari 10 sampel yang diuji menggunakan nested PCR, satu sampel menunjukkan hasil positif untuk L.pneumophila, dan sembilan sampel menunjukkan hasil negatif. Bakteri L. pneumophila ditemukan pada sampel air kolam dengan suhu yang lebih tinggi (>30ºC). Satu sampel positip tersebut ketika dilanjutkan terhadap analisis serogrup terlihat bahwa bakteri L. pneumophila yang terdeteksi pada air kolam renang di Kota Surabaya termasuk L. Pneumophila serogrup 9 (98%) dan serogrup 10 (98%). Terdeteksinya bakteri L. pneumophila ini diharapkan dapat meningkatkan kesadaran dokter dan ahli mikrobiologi tentang penyebaran L. pneumophila dan juga bermanfaat untuk mengontrol agen Legionellosis.
PREVALENCE OF MONOGENEAN HELMINTH ECTOPARASITES ON CATFISH (CLARIAS GARIEPINUS) CULTURE PONDS IN LABAN VILLAGE, MENGANTI DISTRICT, GRESIK REGENCY, EAST JAVA PROVINCE Bendryman, Sri Subekti; Grace, Adelina; Aksono, Eduardus Bimo
Journal of Parasite Science Vol 1, No 1 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (742.648 KB) | DOI: 10.20473/jops.v1i1.16222

Abstract

The aims of this study are to know the prevalence of monogenean helminth ecto-parasites infection on catfish culture, to know determine the number of monogenean infection on 100 samples consists of 1 and 3 months old of catfish. This study was conducted on May to June, 2011 in catfish culture ponds, Laban Village Menganti District and Gresik Regency, East Java Province.   The methods were used in this research were skin and gill scrapping. The data were analyzed by using chi square test with SPSS program version 18.0 for windows. The results showed that prevalence of monogenean in catfish culture ponds, Laban Village Menganti District Gresik Regency, East Java Province was 32%. The highest infection was in the young catfish compared with the older ones with percentage of 23% and 9% respectively and based on chi square test showed that there were a very significantly differences (p < 0.01).