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AKTIVITAS ANTI MYCOBACTERIUM TUBERCULOSIS STRAIN H37RV DAN MDR (MULTI DRUG RESISTANT) DARI EKSTRAK RIMPANG CURCUMA MANGGA VAL.: ANTI MYCOBACTERIUM TUBERCULOSIS ACTIVITY STRAIN H37RV AND MDR (MULTI DRUG RESISTANT) FROM CURCUMA MANGGA VAL. RHIZOME EXTRACT Pratiwi, Ritha; Alam, Gemini; Rante, Herlina; Massi, Nasrum
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 6 No. 1 (2020): (March 2020)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2020.v6.i1.13102

Abstract

TB is an infectious disease caused by the bacterium Mycobacterium tuberculosis. Empirically the plants that have long been used in medicine are Curcuma mangga  rhizome, which shows pharmacological activity as an anti Mycobacterium tuberculosis drug. The objective of the research is to determine the ability of hexane and methanol extracts of Curcuma mangga in inhibiting the growth of Mycobacterium tuberculosis Strain H37Rv and MDR. The extraction of Curcuma mangga rhizome was conducted by maceration methode using hexane and methanol as the solvents, respectively.  Both of extracts were made in 1000 dan 500 ppm concentration, then tested for anti Mycobacterium tuberculosis activity using MODS (Microscopic Observation Drug Suspectibility) method. Determination of anti M.tuberculosis activity was based on microscopic observation on Mycobacterium tuberculosis colony in cord formation. The results showed that hexane and methanol extract in 1000 ppm concentration inhibited the growth of Mycobacterium tuberculosis Strain H37Rv dan MDR. However in 500 ppm only n-hexane  extract showing the inhibiton activity. In conclusion, n-hexane extract have the ability to inhibit the growth of Mycobacterium tuberculosis Strain H37Rv dan MDR. 
ANALISIS HUBUNGAN KUANTITATIF STRUKTUR-AKTIVITAS (HKSA) DARI SENYAWA AKTIF ANTIMALARIA DITERPEN KASSAN HASIL ISOLASI DARI BIJI BAGORE (Caesalpinia crista Linn.) DENGAN PARAMETER ELEKTRONIK Ahmad, Islamudin; Alam, Gemini
Journal of Tropical Pharmacy and Chemistry Vol 1 No 2 (2011): Journal of Tropical Pharmacy and Chemistry
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.953 KB) | DOI: 10.25026/jtpc.v1i2.19

Abstract

ABSTRACT   Quantitative Structure-Activity Relationship (QSAR) study have been conducted to  antimalarial active compounds of cassane-type diterpenes isolated from seed kernels of Bagore (Caesalpinia crista Linn.), using the atomic net charges data resulted from AM1 method of semi-empirical calculations. The aim of this research is to determine the mathematic equation model of quantitative structure-activity relationship from antimalarial active compounds of cassane-type diterpenes isolated from seed kernels of Bagore (Caesalpinia crista Linn.) using the electronic parameter of AM1 method. The best QSAR equation model was determined by multiple linier regression analysis and tested by leave-one-out type of cross validation methods. The result indicates that the biological activity is represented by a linier function of activity versus atomic net charges of C1,  C2, C3, C4, and C6 which is expressed by : Log (1/C) = -7,362(+ 4,251) qC1 +3,258 (+ 4,590) qC2 + 11,038 (+4,485) qC3 + 29,421  (+20,542) qC4 + 19,849 (+6,171) qC6 + 8,541 (+2,256) The equation is significant at 95% level. The cross validation analysis give minimal value of PRESS=64,372 and SEP = 2,93; where Log (1/IC50) = biological activity and q = atomic net charges Key words : QSAR, Atomic Net Charges, Cross Validation Methods   ABSTRAK   Studi hubungan kuantitatif struktur-aktivitas (HKSA) telah dilakukan terhadap 42 senyawa aktif antimalaria turunan diterpen kassan hasil isolasi dari biji Bagore (Caesalpinia crista Linn.) menggunakan data muatan bersih atom hasil perhitungan semiempiris metode (Austin Model 1) AM1. Tujuan penelitian ini adalah menentukan model persamaan matematis hubungan kuantitatif struktur-aktivitas dari senyawa aktif antimalaria turunan diterpen kassan furano hasil isolasi dari biji Bagore (Caesalpinia crista Linn.) dengan parameter elektronik menggunakan metode AM1. Pemilihan model persamaan HKSA terbaik ditentukan dengan analisis regresi multilinier dan diuji dengan metode validasi silang leave-one-out. Hasil penelitian menunjukkan bahwa aktivitas biologis merupakan fungsi linier dari muatan bersih atom C1,  C2, C3, C4, dan C6 yang dinyatakan dalam persamaan : Log (1/C) = -7,362(+ 4,251) qC1 +3,258 (+ 4,590) qC2 + 11,038 (+4,485) qC3 + 29,421  (+20,542) qC4 + 19,849 (+6,171) qC6 + 8,541 (+2,256) Persamaan signifikan pada tingkat kepercayaan 95%. Hasil analisis validasi silang memberikan data parameter pengujian relatif minimal yaitu PRESS = 9,645; dimana Log (1/IC50) = aktivitas biologis dan q = muatan bersih atom   Kata kunci : HKSA, Muatan Bersih Atom, Metode Validasi Silang  
SKRINING DAN ANALISIS KLT-BIOAUTOGRAFI SENYAWA ANTIMIKROBA BEBERAPA EKSTRAK SPONS ASAL PERAIRAN LAUT PULAU BARRANG LOMPO, SULAWESI SELATAN Yulianty, Risfah; Rante, Herlina; Alam, Gemini; Tahir, Akbar
Majalah Obat Tradisional Vol 16, No 2 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.514 KB) | DOI: 10.14499/mot-TradMedJ16iss2pp88-94

Abstract

Spons merupakan salah satu invertebrata filum Porifera yang menghasilkan senyawa aktif dengan berbagai variasi struktur dan salah satu aktivitas biologinya adalah sebagai antimikroba. Penelitian ini bertujuan untuk mengetahui ekstrak spons yang dapat menghambat pertumbuhan mikroba dan potensi senyawa aktif antimikroba dari spons asal perairan Pulau Barrang Lompo. Mikroba uji yang digunakan adalah Escherichia coli, Salmonella typhii, Staphylococcus aureus, dan Candida albicans. Bahan uji diperoleh dengan maserasi 14 buah sampel spons dengan metanol yang dilanjutkan dengan partisi berturut-turut menggunakan kloroform dan metanol, selanjutnya dilakukan uji KLT-bioautografi terhadap ekstrak yang aktif. Hasil penelitian menunjukkan bahwa ekstrak metanol dari sampel spons dengan kode BRLP-009 dan BRLP-010 mempunyai aktivitas paling potensial sebagai antimikroba.
PROTECTIVE EFFECT OF GREEN ALGAE ULVA RETICULATA AGAINST PSEUDOMONAS AERUGINOSA IN DROSOPHILA INFECTION MODEL Nainu, Firzan; Asri, Rangga Meidianto; Djide, M. Natsir; Ahsan, Muhammad; Arfiansyah, Rudi; Sartini, Sartini; Alam, Gemini
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.119 KB) | DOI: 10.4308/hjb.26.4.%x

Abstract

The emergence of antibiotic-resistant Pseudomonas aeruginosa is one of main health issues in global communities. To overcome such threat, the discovery of novel antibacterial agents is indispensable. This study aimed to evaluate the in vivo antipseudomonal activity of Ulva reticulata extract in Drosophila model of infection. Ethanolic extract of Ulva reticulata was prepared using maceration method and the extract was subsequently assessed for its in vivo antibacterial effect against P. aeruginosa using survival assay, bacterial load enumeration, and gene expression analysis in the wildtype Drosophila. Survival and bacterial load analysis were further performed in a similar fashion on the mutant flies devoid of component responsible in the activation of immune responses against P. aeruginosa. Decline in the survival of infected host accompanied by augmentation of bacterial proliferation was documented in the wildtype Drosophila upon infection with P. aeruginosa. These phenotypic events were further amplified in immune-deficient mutant Drosophila. Nevertheless, improvement of host survivorship and reduction of bacterial burden were demonstrated in both wildtype or immune-deficient mutant flies upon treatment with Ulva reticulata extract after bacterial challenge. Our data demonstrated in vivo antipseudomonal activity of Ulva reticulata extract and thus provide a valuable information about its future potential for health promotion.
MOLECULAR MODELING OF VITEOSIN-A, A TRACHEOSPASMOLYTIC COMPOUND ISOLATED FROM N-HEXANE EXTRACT OF THE LEAVES OF Vitex Trifolia L. Alam, Gemini; Sudarmanto, B.S. Ari; Astuti, Puji; Wahyuono, Subagus
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.572 KB) | DOI: 10.14499/indonesianjpharm0iss0pp180-184

Abstract

Viteosin-A, a tracheospasmolytic compound, was successfully isolated from n-hexane extract of the leaves of Vitex trifolia L. With the concentration of 0.05 and 0.15 mg/ml viteosin-A inhibited a guinea pig tracheal contraction due to histamine (10-7 – 10-3 M) in vitro by 27.1 and 47.9 %, respectively. Confirmation of C-5 and C-6 configuration is necessary to determine the active reaction site of viteosin-A and its receptor for future development. This research was focused on a molecular modeling of viteosin-A using computational method with HyperChem Pro 4.0 for Windows as software. Based on spectroscopic data and molecular modeling, viteosin-A has S configuration at C-5 and C-6, and therefore was confirmed as (5S,10S)-6S-acetoxy-8R-methyl-9-hidroxy-labda-13Z-en-16,15-olide.Keywords: viteosin-A, molecular modeling
Purification and characterization of anti-multidrug resistances bacteria from actinomycetes associated sponge Rante, Herlina; ., Wahyono; Murti, Yosi B.; Alam, Gemini
INDONESIAN JOURNAL OF PHARMACY Vol 21 No 3, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.734 KB) | DOI: 10.14499/indonesianjpharm0iss0pp158-165

Abstract

Actinomycetes  are   one  kind  of  the  microorganisms  that  very  important producer  of   secondary  metabolites  for  drugs.Active substances  of  microbial origin  have  been  searched   through  a  series  of  screening  methos  to   obtain novel  compounds  and  strains.  The  purpose  of  this  research  was  to  characterize the  antibacterial  coumpound   from  actinomycetes  associated  sponge  and identification  of  actinomycetes  base  on  morfology  and  fisiology  characteristic. Isolation of actinomycetes from sponge were done bypour and spreap plate. A total  of   actinomycetes  strain  were  isolated  from  sponges  collected   at  Barrang Lompo  island,  Makassar,  South  Sulawesi,   Indonesia. One of  them  showed strong   activity  against  antibiotic  resistant  bacteria   with  concentration 0.0195µg.  Characterisation  of  antimicrobial  coumpound  base  on  IR  spectrum determined  derivate  of  carboxylic   acid.  The  result obtained  from  the morphological  and  physiological  characterisation,  determined  the  strain  a Streptomyces spKey words: sponges, actinomycetes, secondary metabolic, bacteria resistan
Cytotoxicity testing of alkaloid compounds isolated from sponge Petrosia sp: its potency for development of anticancer agent Astuti, Puji; Alam, Gemini; Hartati, Mae Sri; Sari, Dinar; Wahyuono, Subagus
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 1, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.509 KB) | DOI: 10.14499/indonesianjpharm0iss0pp58-62

Abstract

Cancer is still a major problem and common cause of death around the world. Various therapeutic agents have been developed to fight against cancer, but none of these agents give satisfactory results and without debilitating side effects. A number of researches have been conducted to search anticancer compounds with renewed vigour.Sponges, marine invertebrates, are known as rich sources of compounds which pronounced pharmacological activities. The aims of this study are to determine cytotoxic effect of two toxic compounds isolated from chloroform fraction of Petrosia sp sponges collected from Bunaken on myeloma cells.The two toxic compounds were isolated based on bioassay guidedisolation on brine shrimp larvae. Isolation was conducted using column chromatography followed by preparative TLC. Cytotoxic effect of the two compounds was conducted in 96 well plate using RPMI 1640 as medium. The number of viable cells was determined using MTT assay and LC50 (μg/mL) of the compounds was analysed using probit analysis.The results showed that the two compounds were alkaloid and toxic to larva A. salina with LC50 of 7.23 (compound 1) and 5.69 μg/mL (compound 2). These compounds were also toxic to myeloma cells with LC50 values of 16.95 μg/mL (compound 1) and 18.8 μg/mL (compound 2). The longer the incubation time, the compounds were more toxic as showed by the lower LC50 values .Key words: cytotoxic, Petrosia sp, Artemia salina Leach, myeloma
BIOACTIVITY SCREENING OF SPONGES COLLECTED FROM BUNAKEN, MENADO BY Brine Shrimp Lethality Test AGAINST Artemia salina Leach Astuti, Puji; Alam, Gemini; Wahyuono, Subagus
INDONESIAN JOURNAL OF PHARMACY Vol 14 No 1, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.569 KB) | DOI: 10.14499/indonesianjpharm0iss0pp238-243

Abstract

Indonesia is one of the countries rich of natural resources. Sponges are marine invertebrates widely found in Indonesia and known to have various bioactive compounds with unique chemical structures. This study was aimed to screen potentially bioactive extracts of sponges collected from Bunaken and identify on their toxicity level against larva Artemia salina Leach. Samples were macerated using acetone followed by partition using chloroform and methanol. The extracts were prepared at the concentration of 100, 250, 500 and 1000 mg/ml and their toxicity was tested using Brine shrimp Lethality Test. LC50 (mg/ml) of the extracts was calculated using probit analysis. From the 4 extracts tested, all of them showed toxicity to larva Artemia. Chloroform extract of MD-02 was found to be the most toxic with the LC50 of 48.15 mg/ml and considered as a potential candidate for new drug research. Further characterization is still needed for the development in the future. This active sponge was then identified as Petrosia sp.Key words: sponges, Bunaken, bioactivity, Artemia salina Leach
Inhibition of Xanthine Oxidase Activity by Ethanolic Extract of Piperomia pellucida L., Acacypha indica L. and Momordica charantia L. Parawansah, Parawansah; Nuralifah, Nuralifah; Alam, Gemini; Natzir, Rosdiana
The Indonesian Biomedical Journal Vol 8, No 3 (2016)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v8i3.194

Abstract

BACKGROUND: Uric acid is a final result of purine catabolism, the enzymatic reactions in the body cells from amino acids or ribonucleotide dinucleotide. Peperomia pellucida L. (P. pellucida), Acalypha indica L. (A. indica) and Momordica charantia L. (M. charantia) are plants which have efficacy to reduce levels of uric acid excess. The aim of this research is to find out the effect of ethanol extract of P. pellucida, A. indica and M. charantia in preventing the formation of uric acid excess by inhibiting the action of the enzyme xanthine oxidase and comparing the inhibition activity of xanthine oxidase on treatments.METHODS: The study design is experimental and conducted using the enzyme xanthine oxidase, xanthine (substrate), pH 7.5 phosphate buffer, samples (P. pellucida, A. indica and M. charantia ethanol extracts) and HCL as reaction breaker. Inhibition of xanthine oxidase was determined enzymatically and unreacted xanthine was measured by UV spectrophotometer at 290 nm. The data were expressed as percent inhibition and the inhibitory concentration (IC)50 were determined using linear regresion of inhibition activity vs. concentration.RESULTS: The IC50 of P. pellucida, A. indica and M. charantia ethanol extracts in inhibiting xanthine oxidase were 19.5 ppm, 77.6 ppm and 17.8 ppm, respectively. IC50 of allopurinol was 1.99 μg/ml, and negative control (combination of enzyme and substrate) has absorbance value of 0.75026.CONCLUSION: Ethanol extract of M. charantia showed the most potent inhibition toward xanthine oxidase compared to the other two extracts.KEYWORDS: xanthine oxidase, Peperomia pellucida L., Acalypha indica L., Momordica charantia L.
THE EFFECT OF TURMERIC AND GARLIC PHYTOBIOTIC ADDITION WITH DIFFERENT DURATION STORAGE ON THE FUNGAL COLONY IN FEED PURWANTI, SRI; ALAM, GEMINI; A. SYAMSU, JASMAL; AGUSTIN, LAILY; SAPSUHA, YUSRI; LISNAWATY, LISNAWATY
JURNAL PETERNAKAN Vol 13, No 2 (2016): September 2016
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v13i2.2421

Abstract

Turmeric and garlic phytobiotic contain bioactive substances, each of which has a function as an antifungal. The study aims to determined to test the ability turmeric and garlic phytobiotic pressed fungal colonies in the feed with different storage time. The data were analyzed by a factorial experimental design with 5 treatments 4 replications and 2 repetitions. The first factor types phytobiotic and the second factor was storage duration. R0 = basal ration (without phytobiotic), R1 = basal ration + 0.3% synthetic antifungal, R2 = basal ration + 2.5% turmeric, R3 = basal ration + 5% garlic, R4 = (basal ration + 2.5% turmeric+ 5% garlic. W0, W1, W2 and W3 respectively with storage time 0, 2, 4 and 6 weeks. Samples were taken from each experimental unit homogeneously. The results showed the average fungal colonies of garlic phytobiotic treatment (26.06±44.76 CFUx103/g) in 2-week storage time (25.20±22.52 CFUx103/g) lower than the provision of synthetic preservatives (57.75±52.03 CFUx103/g) and the control treatment (74.87±70.69 CFUx103/g). Fungal colonies increases rapidly with increasing length of time of storage. The conclusion of this study was that the additon of turmeric and garlic phytobiotic capable as an antifungal in feed.