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Peningkatan Aktivitas Belajar dan Hasil Belajar Biologi Siswa melalui Strategi Pembelajaran Everyone Is a Teacher Here pada Kelas X SMA Negeri 3 Sengkang Amir, Suharyanti; Ali, Alimuddin; Azis, Andi Asmawati
Biology Teaching and Learning Vol 1, No 2 (2018): Desember
Publisher : Universitas Negeri Makassar

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Abstract

Abstract.  This research is a classroom action research. The purpose of this study improves the activity and student learning outcomes through application of learning strategy Everyone is a Teacher Here. Research subjects are students of class X.I SMA Negeri 3 Sengkang. This research carried out during two cycles. Data collection implemented in the second semester of academic year 2016/2017. This research measure learning activity and learning outcomes. Learning activity measure with observation sheet and learning outcomes measure with written test consist of multiple-choice question as much as 20 items. Student learning activities collected during the learning process observed by 3 observers using observation sheet, then learning outcomes data obtained through evaluation when teaching’s time first and second meeting was done and on third meeting was evaluation.  This matter occurs on each cycle. All the data obtained were analyzed through three ways: qualitative analysis for student learning activities, quantitative for student learning outcomes. Results of research on student learning activity that has been analyzed shows the average in the first cycle of 55.6%, while in the second cycle of 67.2%, so the difference in the increase of 11.6%. Student learning outcomes also increased where the average value on observation data of 69.6, 64.4 the first cycle, and the second cycle 74.8. It can be concluded that the application of learning strategy Everyone is a Teacher Here can increase the activity and student learning outcomes. Keywords: everyone is a teacher here learning strategy, learning activities, learning outcomes.
PURIFIKASI DAN KARAKTERISASI KROM REDUKTASE BACILLUS SP LKA9 Ali, Alimuddin; Soetarto, Endang Sutariningsih; Widada, J. Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.2625

Abstract

Chromate reductase is one of the potential enzymes for hexavalent chrom detoxification. Most of the enzyme is produced by bacteria, especially Bacillus. The aim of this research was to study chromate reductase activity isolated from Bacillus sp LKA9. Bacillus sp LKA9 was isolated from leather tannery liquid waste and used as a model in the experiment. Bacillus sp LKA9 was isolated through enrichment culture using Salt Base Solution containing 3 mM K2CrO4. Chromate reductase was isolated from bacteria by growing on a liquid medium containing chrom hexavalen (Cr VI) through several steps. The first step of the isolation process was to use the precipitated process using ammonium sulphate (30-80%). The next step, crude enzymes from the first step was partially purified through DEAE-Cellulose of Ion Exchange Chromatography Column. Diphenylcarbazide methods was used to examine the activity of enzyme fractions. The result of the experiment revealed that all protein could be precipitated by ammonium sulphate, and the cytoplasmic fraction at saturation of 50-70% showed high enzyme activity. Purified enzymes showed an increase activity 69,385 times to that of crude enzymes. The enzyme optimal had temperature and pH were 350C and 5; respectively. KM of enzyme was 0,0075 mM, and Vmax was 2500 ?mol/minute/mg protein. Enzyme activity was not inhibited by Cu2+, but an ion Ag2+ and Hg2+ inhibited the enzyme activity un-competitive. The activity of enzyme was demonstrated on SDS-PAGE by appearing typically band with molecular weight 29,26 kDa, it was assumed as chromate reductase.
Karakterisasi Mikrobia Rizosfer asal Tanaman Ginseng Jawa (Talinum triangulare) berdasarkan Gen Ribosomal 16S rRNA dan 18S rRNA Ali, Alimuddin; Rante, Herlina
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Publisher : Jurnal Biologi Papua

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Abstract

The rhizosphere is a biologically active zone of the soil around plant roots that contains soil-borne microbes including bacteria and fungi.  The microbes were isolated from rhizosphere soil roots of Java ginseng. The population of microbes was estimated by plate count method. The isolates were identified based on a great variety of morphological, and cultural characteristics. The total of rhizosphere soil microbe population were 20.91(106 cfu.g−1soils) and showed that 12 isolates of bacteria, 15 isolates of actinomycetes, and 10 isolates of fungi which were found in all of soil samples. The molecular analysis of the ribosomal genes showed that the bacterial isolate, actinomycetes and fungi were closely related to of Staphylococcus sp. DGM  (JF923460), Streptomyces avidinii (EU593640) and fungi Aspergillus niger (HQ379853), respectively.Key words: rhizosphere, Java ginseng, 16S rRNA gene, 18S rRNA gene
Karakterisasi Mikrobia Rizosfer asal Tanaman Ginseng Jawa (Talinum triangulare) berdasarkan Gen Ribosomal 16S rRNA dan 18S rRNA Ali, Alimuddin; Rante, Herlina
JURNAL BIOLOGI PAPUA Vol 3, No 2 (2011)
Publisher : JURNAL BIOLOGI PAPUA

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Abstract

The rhizosphere is a biologically active zone of the soil around plant roots that contains soil-borne microbes including bacteria and fungi. The microbes were isolated from rhizosphere soil roots of Java ginseng. The population of microbes was estimated by plate count method. The isolates were identified based on a great variety of morphological, and cultural characteristics. The total of rhizosphere soil microbe population were 20.91(106 cfu.g−1soils) and showed that 12 isolates of bacteria, 15 isolates of actinomycetes, and 10 isolates of fungi which were found in all of soil samples. The molecular analysis of the ribosomal genes showed that the bacterial isolate, actinomycetes and fungi were closely related to of Staphylococcus sp. DGM (JF923460), Streptomyces avidinii (EU593640) and fungi Aspergillus niger (HQ379853), respectively. Key words: rhizosphere, Java ginseng, 16S rRNA gene, 18S rRNA gene
PEMANFAATAN JAMUR ENDOFIT DARI DAUN MURBEI (Morus alba L.) SEBAGAI ANTIBIOTIK Kursia, Sukriani; Ali, Alimuddin; Sami, Fitriyanti Jumaetri; Adhawiyah, Rabiatul
Jurnal Ilmiah As-Syifaa Vol 9, No 1 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

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Abstract

Endophytic fungi are fungi colonize internally plant tissue, without giving detrimental effect to the host plant. They act as symbiont, mediated plant resistance against biotic stress i.e. pests and diasease and abiotic stress such as drought and extreme of temperature. Endophytic fungi are one of the most creative groups of secondary metabolite producers that play important biological roles for human life. Fungi are among the most important groups of eukaryotic organisms that are well known for producing many novel metabolites which are directly used as drugs or function as lead structure for modification.Mulberry (Morus alba L.) is one of the genus species known to have secondary metabolite compound that can be used as an antioxidant, antibacterial, antiviral, antidiabetic, antihypertensive, hypolipidemic, fever, flu, cough, antiemetic and gastrointestinal disorders. This research aimed to determine antibacterial activity of Mulberry Fungi from the leaves against the bacteria Escherichia coli and Staphylococcus aureus. Isolation of endophytic fungal carried by the direct isolation techniques. Isolates which produced as much as 3 isolates the MUR1, MUR2 and MUR3. Further isolates fermented using PDY medium and extracted using ethyl acetate. The ethyl acetate extract qualitative test the activity against bacteria Escherechia coli and Staphylococcus aureus. The result of the antibacteria activity, ethyl acetat extract from fermentation medium PDY of Mulbery (Morus alba L.) inhibited growth of Escherechia coli and Staphylococcus aureus at > 12 mm Keywords: Endophytic fungi, Mulberry (Morus alba L.), Antibiotic.
EFEK AIR REBUSAN KAYU CINA (Lannea coromandelica) TERHADAP HISTOPATOLOGI MUKOSA LAMBUNG MENCIT (Mus musculus) YANG DIINDUKSI ASAM KLORIDA (HCl) Mu'nisa, Andi; Ali, Alimuddin; Junda, M.; Muflihunna, Andi; Istiqamah, Nur
Indonesian Journal of Fundamental Sciences Vol 5, No 1 (2019)
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.74 KB) | DOI: 10.26858/ijfs.v5i1.9370

Abstract

Penelitian ini bertujuan untuk mengetahui efek air rebusan kayu cina terhadap histopatologi mukosa lambung mencit (mus musculus) yang diinduksi asam klorida (HCl). Penelitian ini menggunakan mencit jantan strain ICR sebanyak 45 ekor yang dibagi dalam 3 kelompok perlakuan, yaitu kelompok mencit normal, kelompok mencit gastritis, dan kelompok mencit dengan pemberian air rebusan kayu cina selama 7 dan 14 hari sebanyak 0.3 cc. Hasilnya menunjukkan bahwa setelah pemberian air rebusan kayu cina ada perbaikan sel-sel mukosa lambung mencit. Kesimpulan dalam penelitian ini adalah bahwa air rebusan kayu cina menunjukkan adanya perbaikan secara histopatologis mukosa lambung mencit yang diinduksi asam klorida (HCl).
PENGARUH VARIASI KONDISI FERMENTASI TERHADAP PRODUKSI METABOLIT ANTIBAKTERI EKSTRAK ISOLAT I5 FUNGI ENDOFIT Anredera cordifolia (Ten.) Steenis Ismail, Ismail; Megawati, Megawati; Ali, Alimuddin; Ningsih, Fuji Asmiati
Jurnal Ilmiah Manuntung Vol 5 No 2 (2019): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

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Abstract

Endophytic fungi are one of the microorganisms that live in plants and symbiotic with their host plant produce secondary metabolites. This study aims to determine the optimum growth profile of endophytic fungus isolated from the leaf of Anredera cordifolia (Ten.) Steenis namely isolate I5 as antibacterial. The fungus growth profile optimization analyzed by a correlation between fermentation time and mycelium weight, which collected every 24 hours for 15 days. The media were varied at pH conditions of 4, 7, and 8 in each PDYB medium (glucose and fructose substrates). The results showed that isolate I5 grew optimally on the 13th day on the PDYB medium pH 8. Ethyl acetate extract 5% of isolate I5 could inhibit bacteria growth with the largest inhibition zone diameter against Staphylococcus aureus of 9.96 mm (± 0.60) and Escherichia coli of 9.03 mm (± 0.46).