Dwinna Aliza
Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh

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HISTOPATHOLOGICAL CHANGES OF ERYTHROCYTE AND QUANTITY OF IMMATURE ERYTHROCYTES IN TEGAL DUCKLING (ANAS JAVANICA) INDUCED BY LEAD (PB) Budiman, Hamdani; Aliza, Dwinna
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9792

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The aim of this research is to find out the histopathological changes of erythrocyte and the quantity of immature erythrocyte of tegal duckling treated with lead (Pb). Twenty five young female ducks with the age of 7 days and 60.55.07 g body weight alloted into 4 treatment groups and 1 control group (Ko) with 5 replication each. The treatment group fed with K1 (5.7), K2 (11.4), K3 (17.1), and K4 (22.8) mg Pb/kg body weight for 10 days, respectively. Young ducks showed clinical symptoms such as the color of feces were brown to black, green color of diarrhea, anemic and decreasing body weight. Histopathologically, erythrocyte showed that immature and abnormal in shape (like teardrops, dumb bell, and bottles). The result of variant analysis followed by linier regression shows that the higher the dosage of Pb in diet the higher the quantity of immature erythrocyte andabnormal erythrocyte shape found (P0.01).
EFFECT OF STORAGE TIME OF EPIDIDYMIS AT 5 OC ON SPERMATOZOA QUALITY OF ACEH LOCAL GOAT Hamdan, Hamdan; Panjaitan, Budianto; Sutriana, Amalia; Aliza, Dwinna; Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9811

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The objective of this study was to evaluate the effect of storage time of epididymis at 5 oC on spermatozoa quality of Aceh local goat. This research was conducted at Reproduction Laboratory, FKH Unsyiah from May to June 2009. Nine epididymis from local goat at the age of 1.5-2.0 years were used in this study. The epididymis were collected from abattoir in Banda Aceh. The experimental design used in this study was completely randomized design with 3 treatments and 3 replications. Spermatozoa quality which consist of sperm concentration, motility, amount of life sperm, and sperm abnormality were examined after the collection of epididymis on day 0 (H-0), day 1 (H-1), and day 3 (H-3) post storage at 5 oC. Data were analyzed using one-way analyses of variance (ANOVA). The result showed that no significant difference (P 0.05) seen in the spermatozoa quality after different storage time. The average of spermatozoa concentration on H-0, H-1, and H-3 were 318x107/ml, 282x107/ml, and 241x107/ml respectively. On the average, the percentage of spermatozoa motility on H-0, H-1, and H-3 were 82,27±2,75; 80,25±2,83; and 78,07±0,92%, respectively. Life spermatozoa observed on H-0, H-1, and H-3 were 82,29±2,71; 80,63±1,87; and 80,09±3,31%, respectively. Observation on spermatozoa abnormality showed that the average of spermatozoa abnormality on H-0, H-1, and H-3 were 7,23±0,27; 8,21±0,55; and 10,75±3,14%, respectively. It could be concluded that the spermatozoa quality were not affected by the storage time at temperature of 5 oC.
The Effect of Dipping Time in Natural Methyl Testosterone Hormone on Male Forming Succesiveness Process of Tilapia (Oreochromis niloticus) Larvae Rosmaidar, Rosmaidar; aliza, dwinna; Ramadhanita, Jesica
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.041 KB) | DOI: 10.21157/j.med.vet..v8i2.3361

Abstract

The purpose of this research was to know the influence of dipping time in natural methyl testosterone hormone (MT) on successful male forming process of tilapia (Oreochromis niloticus) larvae. This research used 360 7 days old tilapia larvae, purchased from cultivation of brackish water, Ujung Batee, Banda Aceh. The samples were divided into 4 treatment groups with three repetitions, they were P0 (control group without treatment), PII (Tilapia larvae were dipped in natural MT for 10 hours), PII (Tilapia larvae were dipped in natural MT for 20 hours), and PIII (Tilapia larvae were dipped in natural MT for 30 hours). All tilapia larvae were maintained until the age of 60 days, before the sex of the fish examined. The average percentage of male tilapia in group P0, PI, PII, and PIII were 50.28, 87.77, 90.06, and 95.05%, respectively. Statistical analysis showed that there were significant differences (P<0.05) among P0, PI, PII, and PIII. Based on result of this research, it can be concluded that the longest dipping time the highest the percentage of male tilapia.Key words: larvae, tilapia, natural hormone methyl testosterone, the male sex percentage
IDENTIFICATION OF COPPER-INDUCIBLE GENES IN SWORDTAIL FISH (XIPHOPHORUS SPP.) USING DIFFERENTIAL DISPLAY Aliza, Dwinna; Muhammad, Tengku
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i1.3498

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Differential display technique was used to identify differentially expressed gene ofswordtail fish (Xiphophorus spp.) that induced by 1 µg/ml copper for 24 hours. From eightprimers that have been used for differential display, seven of them showed the differentialgenes which were not present in the control. The reamplification of these fragments rangingfrom below 100 to 200 bp. But only two fragments were successfully cloned and sequenced,those are H-AP10 and H-AP11. Sequence analysis showed that both of the sequences revealedhigh similarity with Homo sapiens stress-associated endoplasmic reticulum protein, a signalgenerated that induces apoptosis.Keywords: differential display, swordtail fish, copper pollution, mRNA expression, biomarker 
CHARACTERIZATION OF INHIBIN FROM CULTURE AND NON CULTURE OF GRANULOSE CELLS FOR MONOCLONAL ANTIBODY OF INHIBIN PRODUCTION Amiruddin, Amiruddin; Nizwan Siregar, Tongku; Sutriana, Amalia; Aliza, Dwinna; Armansyah, T.
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9789

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This study has long-term objectives to obtain immunogenic prototype that can be used to induce multiple ovulation in goats. Working steps of this study were begun with the collection of ovarium from goats, collection of granulose cells, culture of granulose and characterization of molecular weight and isoelectric point (pI) of inhibin protein of granulose cells obtained from culture and non-culture of granulose cells, and followed by preparation of monoclonal antibody toward inhibin. The results showed that inhibin isolated either from culture or non-culture of granulose cells produced a 32 kDa band. Molecular weight of inhibin was measured by Western Blot. The 32 kDa band of SDS PAGE product appeared on Western Blot result was inhibin molecules produced by granulose cells collected fom culture and non-culture of granulose cells that can be identified by Mab-inhibin. Product of IEF gel electrophoresis suggested that inhibin molecule collected from culture of granulose cells has no charge at isoelectric points ranging from 5-6, depends on its total amino acid composition.
DIFERENSIAL LEUKOSIT DAN KETAHANAN HIDUP PADA UJI TANTANG AEROMONAS HYDROPHILA IKAN NILA YANG DIBERI STRES PANAS DAN SUPLEMENTASI TEPUNG DAUN JALOH DALAM PAKAN S, Sugito; N, Nurliana; Aliza, Dwinna; S, Samadi
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2652

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Penelitian ini dilakukan untuk mengetahui efek suplementasi tepung daun jaloh (Salix tetrasperma Roxb) (TDJ) dalam pakan terhadap nilai diferensial leukosit dan ketahanan hidup setelah uji tantang dengan Aeromonas hydrophila pada ikan nila (Oreochromis niloticus) yang dipelihara pada akuarium diberi peningkatan suhu lingkungan. Penelitian ini menggunakan rancangan acak lengkap faktorial 2x4. Faktor pertama adalah penambahan jumlah tepung daun jaloh dalam pakan yaitu: 0% (P1), 5% (P2), 10% (P3), dan 15% (P4) dari berat pakan dan faktor kedua adalah suhu air dalam akuarium yaitu 29±1? C (S1) dan 35±1? C (S2), sehingga didapat 8 kombinasi perlakuan yaitu: P1S1, P2S1, P3S1, P4S1, P1S2, P2S2, P3S2, dan P4S2 dengan ulangan 10 ekor ikan per perlakuan. Sebanyak 80 ekor ikan nila dengan bobot badan 40-50 g secara acak dibagi ke dalam 8 perlakuan. Perlakuan dilakukan selama 30 hari. Pada hari ke 31 dilakukan pengambilan sampel darah dan uji tantang. Hasil penelitian ini menunjukkan bahwa suplementasi pakan dengan TDJ pada konsentrasi 5-15% tidak berpengaruh terhadap nilai diferensial leukosit ikan nila yang dipelihara pada suhu lingkungan yang berbeda. Suplementasi TDJ dalam pakan sebanyak 5-10% dapat mengurangi kematian ikan akibat infeksi Aeromonas hydrophila terutama pada suhu 35±1? C. Dari hasil penelitian ini dapat disimpulkan bahwa ikan nila yang dipelihara pada akuarium dengan suhu air 35±1? C dan diberi pakan yang disuplementasi TDJ 5-10% dapat meningkatkan daya tahan tubuh ikan nila.
UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (PHYLLANTUS EMBLICA) TERHADAP MENCIT (MUS MUSCULUS). (ACUTE TOXICITY TEST OF ETHANOLIC EXTRACT OF MALAKA (PHYLLANTUS EMBLICA) LEAVES ON MICE (MUS MUSCULUS)) TR, T. Armansyah; Indriany, Sudi; Sutriana, Amalia; Rosmaidar, Rosmaidar; Asmilia, Nuzul; Panjaitan, Budianto; Aliza, Dwinna; Hamdan, Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5137

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ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.
EFFECT OF SALINITY CHANGES ON METHIONINE CONTENT IN TIGER GROUPER JUVENILE (EPINEPHELUS FUSCOGUTTATUS) Abbas, Muhammadar Abdullah; Abidin, Muchlisin Zainal; Putra, Dedi Fazriansyah; Firdus, Firdus; Batubara, Agung Setia; Sail, Asmawati Muhammad; Syafruddin, Syafruddin; Roslizawaty, Roslizawaty; Sutriana, Amalia; Aliza, Dwinna
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.12363

Abstract

This study was conducted to determine the effect of water salinity changes on methionine content in tiger grouper juvenile (Epinephelus fuscoguttatus). A total of 2,560 tiger grouper juveniles were used and divided into five groups consist of 1 control group (without exposed to salinity changes) and 4 treatment groups. The salinity was changed every 2, 3, 4, and 6 hours in treatment A, B, C, and D, respectively. Salinity levels were changed during 24 hours by lowering salinity level from 32 psu to 22 psu. Twenty five of fish were collected from each treatment for methionine content analysis. Data were analysed using one way analysis of variance (ANOVA). The results showed that there was a decrease in methionine content in all treatments. A significant decrease (P 0.05) of methionine content in treatment A, B, and C were observed after 20 hours (1.15%), 18 hours (1.27%), and 16 hours (1.24%), respectively. While at 0 hours (control), the methionine content was 2.02%. Methionine level in treatment D was not significantly different (P 0.05) compared to control group. As conclusion, rearing the tiger grouper juvenile with salinity fluctuations every 6 hours did not lead to methionine deficiency.
THE EFFECT OF MALACCA LEAVES (PHYLLANTUS EMBLICA) ETHANOLIC EXTRACT ON PLASMODIUM FALCIPARUM GROWTH IN VITRO Asmilia, Nuzul; TR, T Armansyah; Aliza, Dwinna; Melia, Juli; Rahmi, Erdiansyah; Daulay, Lingga Surya Maret
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.10215

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The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P 0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P 0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.
IDENTIFIKASI PROTEIN IMUNOGENIK TAKIZOIT DAN BRADIZOIT TOXOPLASMA GONDII STRAIN LOKAL UNTUK PENGEMBANGAN KIT DIAGNOSTIK Hanafiah, Muhammad; Aliza, Dwinna
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.559

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Telah dilakukan penelitian untuk mengisolasi dan mengidentifikasi protein 28 kDa (GRA2) dari stadium takizoit dan bradizoit Toxoplasma gondii strain lokal. Cairan asites mencit galur Balb/c digunakan sebagai protein sampel melalui elektroforesis (SDS-PAGE), serum kambing untuk uji antigenitas protein 28 kDa (GRA2) dan serum darah kucing untuk melihat tingkat sensitivitas dan spesifisitas protein 28 kDa (GRA2). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa protein 28 KDa (GRA2) telah dapat diisolasi dari stadium takizoit dan bradizoit Toxoplasma sp. Nilai OD405 antigenesitas ES adalah sebesar 0,578 sedangkan pada antigen GRA2 (28 kDa) diperoleh nilai OD405 adalah sebesar 0,178. Tingkat sensitivitas protein 28 kDa (GRA2) terhadap antibodi dalam serum darah kucing terinfeksi adalah sebesar 61,53% dan nilai spesifisitasnya adalah 33,33%. Tingkat sensitivitas protein ES terhadap antibodi dalam serum darah kucing terinfeksi adalah 76,92% dan tingkat spesifisitasnya adalah 33,33%.
Co-Authors a, Aulanni?am Abbas, Muhammadar Abdullah Abidin, Muchlisin Zainal Amalia Sutriana Amiruddin A Amiruddin Amiruddin Arman Sayuti Asmarida -, Asmarida Basuki B. Purnomo Batubara, Agung Setia budianto panjaitan Dasrul Dasrul Daulay, Lingga Surya Maret Dessy Florenstina BR. G, Dessy Dian Masyitha Dian Pratiwi Eka Janni Haqqawiy, Eka Janni Erdiansyah Rahmi Firdus Firdus Frengki -, Frengki Gholib Gholib, Gholib Hafizuddin Hafizuddin, Hafizuddin Hamdan Hamdan Hamdani Budiman Hamny Hamny I. Ketut Mudite Adnyane, I. Ketut Mudite Iko imelda arisa, Iko imelda Ilham Gianda Tilas, Ilham Gianda Indriany, Sudi Irma Dewiyanti Ismail Ismail Jesica Ramadhanita, Jesica Juli Melia Luky Wahyu Sipahutar, Luky Wahyu M Nur Salim, M Nur M. Aris Widodo Mahdi Abrar Muhammad Hambal Muhammad Hanafiah Muhammad Jalaluddin, Muhammad Mulia, Hattanul Mulya Fahmi, Mulya Mulyadi Adam, Mulyadi Murliana, Baida Muslim Akmal Nazaruddin - Nazaruddin N Nazarudin, Nazarudin Nellita Meutia, Nellita Nova Lianda, Nova Nurliana N, Nurliana NURLIANA NURLIANA Nuzul Asmilia Oktarian, Alfin Putra, Dedi Fazriansyah R, Rahmiwati Rastina, Rastina Razali Daud Rika Anggraini Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar, Rosmaidar Rusli Rusli Saiful muna, Saiful Sail, Asmawati Muhammad Samadi S, Samadi Siska Mellisa, Siska Siti Aisyah Sofyatuddin Karina Sri Wahyuni Srihadi Agungpriyono Sugito - Sugito s Sugito Sugito Sutiman B. Sumitro Syafrizal S Syafruddin s Syafruddin Syafruddin T. Armansyah T. Armansyah TR, T. Armansyah Tarmizi Tarmizi Tengku Muhammad, Tengku Teuku Adelin, Teuku Teuku Armansyah Teuku Fadrial Karmil Tongku Nizwan Siregar Tongku Nizwan Siregar TR, T Armansyah Ummu Balqis Vara Tassa Sutari, Vara Tassa Winaruddin - Wisnu Nurcahyo Yakin Ikhwan, Yakin Yudha Fahrimal Yulanda, T. Elisa Zainal Muttaqin Zainuddin Z Zuhrawati Zuhrawati, Zuhrawati