. ANDI
Faculty of Mathematics and Natural Sciences, Universitas Indonesia

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Archaeal Life on Tangkuban Perahu- Sampling and Culture Growth in Indonesian Laboratories HANDAYANI, SRI; SANTOSO, IMAN; FREISLEBEN, HANS-JOACHIM; HUBER, HARALD; ANDI, .; ARDIANSYAH, FERY; MULYANTO, CENMI; LUTHFA, ZESSINDA; SALEH, ROSARI; FREISLEBEN, SERUNI KUSUMA UDYANINGSIH; WANANDI, SEPTELIA INAWATI; THOMM, MICHAEL
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.597 KB) | DOI: 10.4308/hjb.19.3.150

Abstract

The aim of the expedition to Tangkuban Perahu, West Java was to obtain archaeal samples from the solfatara fields located in Domas crater. This was one of the places, where scientists from the University of Regensburg Germany had formerly isolated Indonesian archaea, especially Thermoplasma and Sulfolobus species but not fully characterized. We collected five samples from mud holes with temperatures from 57 to 88 oC and pH of 1.5-2. A portion of each sample was grown at the University of Regensburg in modified Allen’s medium at 80 oC. From four out of five samples enrichment cultures were obtained, autotrophically on elemental sulphur and heterotrophically on sulfur and yeast extract; electron micrographs are presented. In the laboratories of Universitas Indonesia the isolates were cultured at 55-60 oC in order to grow tetraetherlipid synthesizing archaea, both Thermoplasmatales and Sulfolobales. Here, we succeeded to culture the same type of archaeal cells, which had been cultured in Regensburg, probably a Sulfolobus species and in Freundt’s medium, Thermoplasma species. The harvested cells are documented by phase contrast microscope equipped with a digital camera. Our next steps will be to further characterize genetically the cultured cells from Tangkuban Perahu isolates.
KONDISI KIMIA TANAH PADA LAHAN AGROFORESTRI DAN KEBUN CAMPURAN DI NGATA KATUVUA DONGI-DONGI KECAMATAN PALOLO KABUPATEN SIGI SULAWESI TENGAH Andi, .; Wardah, .; Toknok, Bau
Jurnal Warta Rimba Vol 6, No 3 (2018)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.373 KB)

Abstract

This study aims to determine the soil chemical properties in cacao agroforestry and cacao mixed garden. The research was conducted for three months, October – December, 2017, at Ngata Katuvua Dongi-Dongi, Palolo Sub-district, Sigi District, Central Sulawesi. The study used a survey method, soil samples collected on five points at 0-30cm and 30-60cm depth for each plot, and then compiled into one sample. The soil samples collection are 4 soil sample. The soil samples were analyzed at the Laboratory of Soil Science Unit of the Faculty of Agriculture, Tadulako University. The study results showed that soil chemical properties on cacao agroforestry tend to be better than soil on cacao mixed garden. The Soil chemical properties on cacao agroforestry: pH 6.42, N-total 0.27%, P-available 13.79ppm, K-available 0.26 (cmol (+) kg-1, C-organic 3.69% and CEC 22,34 (cmol (+) kg-1. While the soil chemical properties on the cacao mixed garden: pH 6.20, N-total 0.11%, P-available 6.67ppm, K-available 0.12 (cmol (+) kg-1, C-organic 1.84%, and CEC 13.45 (cmol (+) kg-1.Keywords: soil chemistry, agroforestry, mixed garden.