Sintho Wahyuning Ardie
Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti Kampus IPB Darmaga, Bogor 16680, Indonesia

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APLIKASI BERBAGAI KOMPOSISI DAN KONSENTRASI PUPUK MAJEMUK UNTUK PEMBENTUKAN KANTONG PADA NEPENTHESX VENTRATA Saputro, Romy Agus; Ardie, Sintho Wahyuning; Krisantini, .
Buletin Agrohorti Vol. 1 No. 1 (2013): Januari 2013
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.923 KB) | DOI: 10.29244/agrob.1.1.113-118

Abstract

Nepenthes is a genus of carnivorous plants in the monotypic family Nepenthaceae. The genus comprises around 130 species, numerous natural and many cultivated hybrids. Plants generally need fertilizer to promote their growth and development. However, in their original habitat Nepenthes forms pitchers under nutrition starvation condition. This study aimed at determining the effect of fertilization on the growth and pitcher formation of Nepenthesx ventrata. Experiment was conducted at Suska Nursery, Caringin, West Java, Indonesia from February until June 2011. Plants were treated with the N: P: K, 60-30-30, 32-10-10 and 10-55-10 and at different concentrations ie, 0.5, 1 and 2 g L-1. Fertilizer was applied weekly to the growing media. Weekly observations were conducted on number of leaves, plant height, time of pitchers initiation, the number of pitchers and pitcher diameter and length. Daily temperature, relative humidity and EC (electrical conductivity) of the growing media were also recorded during the experiment. The results showed that combination of fertilizer with different composition and concentration did not affect Nepenthes growth and development. Application of fertilizer resulted in smaller size pitchers compared to control plants. Future research on Nepenthes should be conducted in a longer period since Nepenthes is a slow growing plants.Keywords: Nepenthesx ventrata, fertilizer, pitcher plant
PEMBENTUKAN BUAH DAN PERKECAMBAHAN TANAMAN JARAK PAGAR PADA TRANSFORMASI LANGSUNG MELALUI JALUR TABUNG POLEN Zainudin, Agus; Purwoko, Bambang Sapta; Santoso, Tri Joko; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.449 KB) | DOI: 10.24831/jai.v46i1.12897

Abstract

The genetic transformation via pollen-tube pathway in jatropha is the first alternative method that was applied in this plant. The objective of the research was to study fruit set and germination of three genotypes of jatropha following direct transformations via pollen-tube pathways. The research was conducted during April 2014 until January 2015 at jatropa?s experimental field, Pasuruan, and at green house of ICABIOGRAD, Bogor. Three genotypes of jatropha i.e., IP3A, IP3P and JcUMM18 were used. In the first experiment, split plot design was used where 3 levels of DNA plasmid concentration as a main plot and 5 levels of stigma-drip time of DNA plasmid as a subplot. In the second experiment randomized block design was used with single factor consisting of 15 combinations of concentration and stigma-drip time of DNA plasmid as treatments and control. The results demonstrated that interaction between concentration and application time of DNA plasmid did not significantly affect fruit and seeds formation of three Jatropha genotypes. Combination of DNA plasmid concentration with time of stigma-drip had significant effect on seed germination rate of IP3A genotype, but not significant on the other variables. The concentration of 0.05-0.5 µg µL-1 and application time of DNA plasmid at 1-10 hours after pollination could be applied on jatropha genetic transformation via pollen-tube pathways.Keywords: DNA plasmid, Jatropha curcas, pCAMBIA1301, pollen-tube, stigma-drip
RADIOSENSITIVITAS DAN KERAGAMAN UBI KAYU (MANIHOT ESCULENTA CRANTZ) HASIL IRADIASI SINAR GAMMA Maharani, Sadewi; Khumaida, Nurul; Syukur, Muhamad; Ardie, Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.061 KB) | DOI: 10.24831/jai.v43i2.10412

Abstract

ABSTRACTHigh genetic variability is one of important determinants in successful breeding of clonally propagated crops such as cassava. Induced mutation, including mutagenesis using gamma irradiation, is one strategy to increase genetic variability. The objective of this research was to obtain information of lethal doses (LD20-LD50) from five cassava genotypes (Jame-jame, Ratim, UJ-5, Malang-4, and Adira-4), to analyze the variability, and to obtain potentially high yielding cassava mutant candidates. Cuttings of several cassava genotypes were irradiated by 0, 15, 30, 45, and 60 Gy gamma rays. The results showed that genotype Malang-4 had the lowest LD20 (7.53 Gy) and LD50 (18.47 Gy), while genotype Jame-jame had the highest LD20 (24.94 Gy) and LD50 (33.24 Gy). The highest phenotype variability was obtained in the cassava population irradiated by 15-30 Gy gamma rays. The highest phenotype variability was also determined by the source of tissue, i.e., cuttings from the middle stem resulted in the highest phenotype variability. This research found 32 high yielding potential candidate mutants.Keywords: gamma rays, LD20, LD50,mutant, mutation
PERANAN FOSFOR DALAM MENINGKATKAN TOLERANSI TANAMAN SORGUM TERHADAP CEKAMAN ALUMINIUM Lestari, Tri; ,, Trikoesoemaningtyas; Ardie, Sintho Wahyuning; Sopandie, dan Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.43 KB) | DOI: 10.24831/jai.v45i1.13814

Abstract

The management of P nutrition can be a good solution for aluminum toxicity and P nutrient deficiency in acid soil. This study aimed to determine the role of phosphorus in improving the tolerance of sorghum to Al stress in nutrient culture. This research was conducted at green house of IPB Bogor, tissue culture laboratory in the Department of AGH IPB and Balai Besar Pasca Panen Cimanggu Bogor, from January to November 2014. A completely randomized factorial design was used in three experiments. The results revealed that addition of P improved the tolerance of two sorghum genotypes to Al stress as indicated by the reduction in root length inhibition by Al, where  P was more effective in tolerant genotype Numbu. Addition of P reduced the accumulation of Al in the root tissues as shown by the lighter intensity of hematoxylin staining, especially in Numbu. Aluminum stress increased the secretion of oxalate acid in both sorghum genotypes, where P lowered oxalate acid secretion in both genotypes. These facts showed that the role of P in improving the tolerance of sorghum to Al stress might be associated with the inhibition of Al absorption into the root tissues, although the mechanism is not yet known.Keywords: Al stress, organic acid secretion, role of P, root staining method, sorghum
KARAKTERISASI MORFOLOGI DAN SIFAT KUANTITATIF GANDUM (TRITICUM AESTIVUM L.) DI DATARAN MENENGAH Widowati, Sartika; Khumaida, Nurul; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.256 KB) | DOI: 10.24831/jai.v44i2.13485

Abstract

ABSTRACTIndonesia is one of the largest wheat importers. Suitable environmental condition for wheat needs to be studied if wheat is going to be widely cultivated in Indonesia. The adaptability of wheat grown in various climates and altitudes is one of the important aspects. The objective of this experiment was to study the quantitative and morphological character of wheat grown in middle land (540 m asl) in Bogor, West Java. The experiment was arranged in randomized complete block design with three replications. Wheat genotypes used were three national varieties (Nias, Selayar, and Dewata), four new improved varieties (Guri 3 Agritan, Guri 4 Agritan, Guri 5 Agritan, and Guri 6 Unand), and one introduced genotype (SBD). Data were collected for several quantitative variables and seventeen morphological characters based on UPOV descriptor. The result showed that wheat growth was restricted in Bogor. Genotype determined plant height, leaf number, ear length, root length, number of spikelet, harvest time, seed weight, number of tillers, and plant biomass. Based on ear length, grain weight, and plant biomass, Guri 3 Agritan had the highest production than the other genotypes.Keywords: diversity, genetic relationship, high temperature, introduced genotype, phylogenetic
POLA AKUMULASI PROLIN DAN POLIAMIN BEBERAPA AKSESI TANAMAN TERUNG PADA CEKAMAN KEKERINGAN Kurniawati, Siti; Khumaida, Nurul; Ardie, Sintho Wahyuning; Hartati, N. Sri i; Sudarmonowati, Enny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.049 KB) | DOI: 10.24831/jai.v42i2.8432

Abstract

ABSTRACT Drought stress is one of the major limiting factors for plant growth and production. Therefore, the mechanism of drought tolerance is important to be studied. Eggplant (Solanum spp.) is relatively tolerant to drought stress compared to other member of Solanaceae family. The objective of this study was to study the mechanism of drought tolerance in eggplant related to accumulation pattern of proline and polyamines. Seven eggplant accessions were drought stressed for 21 days and rewatered for the following next 7 days. Drought stress treatment significantly decreased the soil water content and reduced plant height of all accessions. Eggplant responded the drought stress by reducing stomatal density and stomatal opening to reduce transpiration rate. Drought stress also induced proline and polyamine (PA) accumulation in the leaf tissues. These accumulations started at 14-days after drought stress period when soil water content less than 20% and reached their peaks at 21 days after drought stress. The highest level of proline (134.70 ?mol g-1) and that of putrescine (20.836 ng g-1)  could  be used as indicators for eggplant drought stress condition. Keywords: physiological responses, polyamine (PA), proline, putrescine, stomata
UJI CEPAT TOLERANSI TANAMAN PADI TERHADAP CEKAMAN RENDAMAN PADA FASE VEGETATIF ,, Yullianida; Ardie, Sintho Wahyuning; Aswidinnoor, Hajrial
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.889 KB) | DOI: 10.24831/jai.v42i2.8423

Abstract

ABSTRACTThe major constraint of rice cultivation in the flood-prone area is the lack of tolerant varieties. A rapid screening method have to be developed under controlled environments, such as a green house, to improve the effectiveness and efficiency of the selection under submergence condition. The aim of this research was to study the correlation beetween selection methods in greenhouse and in the field. This experiment was conducted at a green house of Muara Experimental Station, Bogor in 2011/2012. The experimental designs used were randomized complete design for greenhouse experiment and randomized complete block design for field experiment, with three replicates respectively. There were four sub-experiments in the greenhouse experiment, grouped based on seedling age (10 days and 35 days after seeding) and duration of submergence (10 days and 14 days). Those sub-experiments in the green house were compared with the experiment in the field. The results showed that there was strong correlation between recovery percentage in the green house and percentage recovery in the field. The same correlation indeed happen between recovery percentage in the green house to grain yield. Complete submergence at 10-day-old seedlings in green house could be developed as rapid selection method for submergence tolerant rice.Keywords: aciotic stress, correlation, flooding, Oryza sativa
PERTUMBUHAN DAN PERKEMBANGAN UBI KAYU GENOTIPE LOKAL MANGGU PADA PANJANG SETEK BATANG YANG BERBEDA Siswati, Leni; Ardie, Sintho Wahyuning; Nurul Khumaida
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 3 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.116 KB) | DOI: 10.24831/jai.v47i3.26610

Abstract

Cassava plant is widely cultivated by Indonesian people. Research on the development of roots and tubers of cassava is still limited. The purpose of this study was to evaluate the effect of stem cut size on the root development system and tuber filling in the cassava local genotype ?Manggu?. This study consisted of two experiments namely experiments in polybag and in the field. The study was conducted in the IPB experimental field at Cikabayan in December 2017-July 2018. The experiment used a randomized completely block design (RCBD) with two treatments and three replications, namely cuttings of 15 cm and 30 cm. Observation of basal roots, number of nodal roots, length of basal roots, length of nodal roots, number of tubers, number of leaves, plant diameter, and plant height were carried out destructively every week. The results showed that the root bulking process occurred in the nodal root and basal roots and it was observed since 5 WAP. The results showed a positive correlation between the total number of tubers and the number of tubers formed from nodal and basal roots, the number of basal roots, and the length of basal roots and it was negatively correlated with the number of leaves. The number of tubers from nodal roots is positively correlated with the number of basal root tubers while it is negatively correlated with the length of the nodal root. The development of roots into cassava tubers occurred since 5 WAP. Cutting size treatments significantly did not affect root development. Keywords: basal root, nodal root, stem cutting, stem length
HERITABILITAS, KARAKTERISASI, DAN ANALISIS CLUSTERGRAM GALUR-GALUR PADI DIHAPLOID HASIL KULTUR ANTERA Anshori, Muhammad Fuad; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Ardie, Sintho Wahyuning; Suwarno, Willy Bayuardi; Safitri, dan Heni
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.968 KB) | DOI: 10.24831/jai.v46i2.18377

Abstract

Doubled haploid rice formation through anther culture becomes one of the alternatives in the plant breeding. The lines need to be characterized, clustered, and selected. This study aimed at determining heritability, characters, and cluster of doubled haploid lines obtained from anther culture. This experiment was conducted at IPB Rice Field Experiment Station, Darmaga, Bogor from October 2016 until January 2017. The experiment was managed according to randomized complete block design (RCBD) with 3 replications and genotype as the single factor treatment. Genotypes used were 60 genotypes consisting of 56 doubled haploid lines and 4 control varieties. The controls were Ciherang, Inpara 5, Inpari 29, and Inpari 34 Salin Agritan. The results showed that all characters have significant effect and high heritability (above 60%), so they could be used as a selection character. The tested genotypes could be grouped into three main groups. The first group consisted of 11 genotypes, the second group consisted of 44 genotypes, and the third group consisted of 5 genotypes. The main group determinant characters were productive tillers, percentage of filled grain number, plant height, panicle length, and weight of 1,000 grains. The number of filled grain and productive tillers can be used as selection characters together with yield potential.
EVALUASI HASIL DAN KANDUNGAN PATI MUTAN UBI KAYU HASIL IRADIASI SINAR GAMMA GENERASI M1V4 Subekti, Isnani; Khumaida, Nurul; Ardie, Sintho Wahyuning; Syukur, dan Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.196 KB) | DOI: 10.24831/jai.v46i1.17610

Abstract

Cassava is one of the important starch producer crops. Although Indonesia ranks as the third largest cassava producer country, Indonesia still imports cassava starch to meet the domestic demand in food and non-food industries. Gamma ray irradiation has been implemented to generate cassava mutants in order to support development of superior cassava variety, especially high yield and high starch content, especially high yield and high starch content. The objectives of this research were to evaluate the yield potential and the starch content of gamma irradiated 40 putative cassava mutants at M1V4 generation. This research was arranged in randomized complete block design with three replications and conducted at Cikabayan Experimental Field, IPB, Bogor, from May 2016 to March 2017. The starch content was measured using gravimetric methods. The results showed that five mutants, G2142, G3151, G2141, G1143, and G3111, had higher tuber weight per plant (6-7 kg per plant) compared to the background ?Gajah? genotype (4.7 kg per plant). ?G2112? mutant genotype showed higher starch content (23.86%) compared to the background ?Gajah? genotype (17.65%). The specific gravity (SG) was positively correlated with starch content (r = 0.905), indicated that the higher the SG, the higher the starch content of cassava.Keywords: gravimetric, mutant, specific gravity (SG), yield