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BIOCHEMICAL STUDY OF STRIPED CATFISH PANGASIANODON HYPOPHTHALMUS BROODSTOCK INDUCED BY PMSG HORMONE + ANTI‒DOPAMINE AND TURMERIC ADDITION Arfah, Harton; Sudrajat, Agus Oman; Suprayudi, Muhammad Agus; Junior, Muhammad Zairin
Jurnal Akuakultur Indonesia Vol. 17 No. 2 (2018): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3416.018 KB) | DOI: 10.19027/jai.17.2.191-198

Abstract

ABSTRACT This study aimed to evaluate biochemical changes (cholesterol, triglyceride, HDL, LDL, glucose, and plasma protein) on striped catfish Pangasianodon hypophthalmus broodstock induced with PMSG hormone and turmeric addition. An observation was also done to blood glycogen content. The striped catfish broodstock was fed on commercial feed without any addition (control) and with turmeric addition (HKu). In control treatment, there was a decreasing on cholesterol, meanwhile, the triglyceride (TG) value was increased. The HDL concentration was decreased in 2nd sampling and increased in 4th sampling. In 1st until 4th sampling, glucose was quite stable, while LDL was on extremely low concentration. In HKu treatment, the cholesterol value was higher than the control treatment. The TG concentration also higher than control in 3rd sampling and decreased in 4th sampling. The HDL concentration was increased and higher than the control treatment, while LDL concentration was lower. The liver glycogen content on the control and HKu treatment were 0.015 (mg/100 mL) and 0.181 (mg/100 mL) respectively; while in the flesh of the control and HKu treatment were 0.76 (mg/100 mL) and 1.19 (mg/100 mL) respectively; and in the gonad of control and HKu treatment were 0.10 (mg/100 mL) and 0.70 (mg/100 mL) respectively. It was shown that the glycogen content in the liver, flesh, and gonad on experimental fish was higher than control treatment. Keywords : biochemistry, hormone, turmeric, channel catfish, reproduction  ABSTRAK Penelitian ini bertujuan untuk mengkaji perubahan biokimia (kolesterol, trigliserida, HDL, LDL, glukosa dan protein plasma) induk ikan patin Pangasianodon hypophthalmus yang diberi perlakuan hormon PMSG dan kunyit (HKu). Pengamatan juga dilakukan terhadap glikogen dalam darah induk patin. Induk ikan patin diberi pakan tanpa penambahan kunyit (kontrol) dan pakan yang diberi HKu. Hasil penelitian pada kontrol menunjukkan adanya penurunan kolesterol, sedangkan pada TG mengalami peningkatan. HDL menurun pada sampling ke?2 dan meningkat pada sampling ke?4. Sementara itu, pada LDL rendah sekali dan pada glukosa terlihat stabil dari sampling ke?1 sampai ke?4. Pada perlakuan HKu terlihat bahwa pada kolesterol menghasilkan nilai lebih tinggi dibanding kontrol. Pada TG terlihat juga nilai lebih tinggi dibanding kontrol pada sampling ke?3 dan menurun pada sampling ke?4. Konsentrasi HDL meningkat dan lebih tinggi dibanding kontrol, sedangkan nilai LDL lebih rendah. Data yang diperoleh pada kadar glikogen hati perlakuan kontrol adalah 0,015 (mg/100 mL) dan HKu 0,181 (mg/100 mL); sedangkan pada daging kontrol sebesar 0,76 (mg/100 mL) dan HKu 1,19 (mg/100 mL); serta gonad kontrol 0,10 (mg/100 mL) dan HKu 0,70 (mg/100 mL). Hal ini menunjukkan kadar glikogen pada hati, daging, dan gonad ikan yang diberi perlakuan bernilai lebih tinggi dibanding kontrol.           Kata kunci : biokimia, hormon, kunyit, ikan patin, reproduksi  
EFFECT OF ELECTRICAL FIELD ON GONADAL DEVELOPMENT OF GOLDFISH IN SALINE MEDIA Nirmala, Kukuh; Habibie, Ahmad; Arfah, Harton
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2938.394 KB) | DOI: 10.19027/jai.14.9-17

Abstract

ABSTRACT The aim of this study was to evaluate the effect of electric field exposure duration at the voltage of 10 volt on goldfish Carassius auratus auratus gonadal development maintained in 3 ppt salinity media. The experiment consisted of four treatments in triplicates i.e. control, two, four, and six minutes of electrical-field exposure. The experiment design used was completely randomized design. Fish used was female goldfish at the density of 4 fish/aquarium with an average total length of 12.27±0.05 cm and average body weight of 22.29±0.54 g. Result of study showed that the electrical-field exposure at 10 volt for all duration treatments in 3 ppt of media salinity did not give significant effect on gonadosomatic index (GSI) and gonadal development of goldfish. Keywords: electrical field, Carassius auratus auratus, gonad, salinity  ABSTRAK Tujuan penelitian ini adalah untuk mengevaluasi pengaruh lama waktu pemaparan medan listrik dengan voltase 10 volt terhadap perkembangan gonad ikan komet Carassius auratus auratus yang dipelihara pada media bersalinitas 3 ppt. Perlakuan pada penelitian ini terdiri atas empat perlakuan, yaitu: perlakuan kontrol, dua, empat, dan enam menit. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Ikan uji yang digunakan adalah ikan komet betina. Jumlah ikan yang digunakan adalah 4 ekor/akuarium dengan panjang total rata-rata 12,27±0,05 cm dan bobot tubuh rata-rata 22,29±0,54 g. Hasil penelitian menunjukkan bahwa pemberian medan listrik sebesar 10 volt dengan lama waktu pemaparan medan listrik pada semua perlakuan durasi di media pemeliharaan bersalinitas 3 ppt tidak memberikan pengaruh yang nyata terhadap parameter indeks gonadosomatik (GSI) dan perkembangan gonad ikan komet. Kata kunci: medan listrik, Carassius auratus auratus, gonad, salinitas
UTILIZATION OF MS 222 IN TRANSPORT OF CATFISH (PANGASIUS SUTCHI) SEED Arfah, Harton; Supriyono, Eddy
Jurnal Akuakultur Indonesia Vol. 1 No. 3 (2002): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.028 KB) | DOI: 10.19027/jai.1.119-122

Abstract

ABSTRACTProblems faced in supplying seed out of Java especially Bogor to Sumatera, for instance, are it only contains few seed (100 seeds/l) and it has a high mortality rate because of stress during transport.  This experiment was conducted to know the effect of MS 222 in keeping fish metabolism activity down while taking note of its survival rate and change of water quality. The experimental design used here was factorial design 3x5 with 3 repetitions.  Dosages of MS?222 were 0, 25 and 50 ppm and the fish densities were 100, 300, 400, 500 and 600 per liter.  As container was plastic bag (volume 10 l).  After putting the fish into the plastic bag, it was filled up with oxygen, which the volume was 3 times than water volume, than the plastic bag was closed by tying it.  After 18 hours of treatment the survival rate and water quality were checked.  Based on evaluation of survival rate, it was concluded that the combination between 25 ppm of MS 222 and fish density, which was 500 per liter, gave an optimum result.Keywords: Catfish, Pangasius sutchi, live transport, stress, MS?222, survival rate ABSTRAKKendala yang dihadapi dalam pemasokan benih ikan patin dari pulau Jawa terutama Bogor, keluar pulau Jawa terutama Sumatera adalah jumlah ikan yang terangkut masih sedikit (± 100 ekor/l) dan tingkat kematian yang cukup tinggi, karena stres selama perjalanan. Penelitian ini dilakukan untuk mengetahui pengaruh penggunaan MS?222 dalam menekan aktivitas metabolisme ikan dengan memperhatikan tingkat kelangsungan ikan dan perubahan kualitas air. Penelitian ini menggunakan rancangan faktorial 3x5 dengan 3 ulangan. Dosis MS?222 yang digunakan: 0,25 dan 50 ppm dan kepadatan ikan: 100, 300, 400, 500 dan 600 ekor/l. Wadah pengangkutan berupa kantong plastik (volume 10 l). Ikan yang diangkut dimasukkan ke dalam kantong plastik, kemudian dipompakan oksigen sebanyak 3 kali volume air, selanjutnya dilakukan pengikatan. Setelah 18 jam perlakuan dilakukan perhitungan terhadap tingkat kelangsungan hidup ikan dan perubahan kualitas air media. Hasil penelitian ini menunjukkan kombinasi penggunaan MS?222 25 ppm dan kepadatan ikan 500 ekor/l memberikan tingkat kelangsungan hidup yang optimal.Kata kunci: Ikan patin, Pangasius sutchi, pengangkutan hidup, stres, MS?222, tingkat kelangsungan hidup.
SEX REVERSAL ON CONGO TETRA FISH (MICRALEPTUS INTTERRUPTUS ) LARVAE Arfah, Harton; Alimuddin, ,; Sumantadinata, K.; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.65 KB) | DOI: 10.19027/jai.1.69-74

Abstract

ABSTRACTExperiment was performed to assess the effect of 17a-methyltestosterone (MT) treatment on Congo tetra fish larvae.  To evaluate the optimal pattern of MT treatment, three different treatments were administrated.  Three months old larvae were submerged in three different doses of MT; 1, 2 and 4 mg/l.  These studies showed that the highest percentage of male fish was obtained by 4 mg/l MT treatment, 87,17%.  The 2 mg/l and 1 mg/l MT treatments obtained 77,53% and 69,86% male respectively, two times higher than control, 38,96%.  On the other hand, the 4 mg/l MT treatment also resulted the highest percentage of hermaphrodite fishes, 17,58%.  The highest survival rate was shown by 1 mg/l MT treatment, 62,77% and the lowest was shown by the 4 mg/l MT treatment, 47,20%.  The highest rate of fish length and weight was shown by the 4 mg/l MT treatment, 4,4 cm and 1,65 gram respectively.  These findings suggest that MT treatment offers an advantage in growth of  tetra Congo larvae. Key word :  Sex reversal, methyltestosterone, Congo tetra fish, Micraleptus intterruptus. ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh perendaman larva di dalam larutan hormon 17a-metiltestosteron pada dosis 1, 2 dan 4 mg/l larutan.  Persentase tertinggi ikan jantan dihasilkan  oleh perlakuan 4 mg/l, yaitu 87,17%.  Perlakuan 2 mg/l dan 1 mg/l masing-masing menghasilkan 77,53% dan 69,86% sedangkan kontrol menghasilkan 38,96% jantan.  Efek lain dari perlakuan MT ini adalah hermafroditisme.  Perlakuan 4 mg/l menghasilkan persentase hermafrodit tertinggi yaitu 17,58%, sedangkan pada kontrol kelangsungan hidup tertinggi diperoleh pada perlakuan 1 mg/l (62,77%) dan terendah pada perlakuan 4 mg/l (47,20%).  Hasil tersebut menunjukkan adanya pengaruh dosis hormon terhadap kelangsungan hidup ikan.  Pengukuran bobot dan panjang ikan pada setiap perlakuan menunjukkan nilai tertinggi dihasilkan oleh perlakuan 4 mg/l  yaitu 1,65 gram dan 4,40 cm.  Hal ini diduga bahwa hormon MT merangsang pula pertumbuhan ikan.Kata kunci :  Pergantian kelamin, metiltestosteron, ikan tetra Kongo, Micraleptus intterruptus.
QUALITATIVE AND QUANTITATIVE CHARACTERS OF THREE COMMON CARP (CYPRINUS CARPIO L.) STRAINS IN INDONESIA Arfah, Harton; Soelistyowati, Dinartri
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 11 No. 2 (2004): Desember 2004
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.652 KB)

Abstract

The second generation of gynogenetic diploid common carp of majalaya, sinyonya and mirror strains have been produced in 1992. Some of them were sex-reversed by oral administration of 17 ?-methyltestoteronne. The females and sex-reversed males were matured on early 1994 and mated to produce progenies ofpure strains. This research aimed to measure certain quantitative characters since the early stage of developmentto the market size. Three different conditions of rearing (i.e. rice field, ponds and concrete tanks) were applied during the nursery phase, while the earthen pond and running water system with intensive feeding were used for the grow-out stage. The results showed that average fecundity of sinyonya carp was higherthan mirror and majalaya strains, but its performances of the early development (i.e. fertilization rate, hatching  rate and survival rate at 14 days old) was lowest. At the nursery phase, the survival and growth rates of majalaya carp was relatively better than the other two strains, but the mirror carp had highest feed efficiency and lowest protein retention. During culture period, the growth rates of these three common carp strains were relatively similar (1%), but sinyonya carp, among strains had the highest percentage of fillet (38.5%) with the  lowest total number of muscular bones (69), the highest level of protein content (77.5%) and the lowest level  of lipid content (16.6%) among the three strains. Keywords: Cyprinus carpio L, gynogenesis, growth rate, product quality.
MOLECULAR MARKER IS USEFUL IN THE DEVELOPMENT OF TESTICULAR CELLS TRANSPLANTATION FOR DETECTING DONOR-DERIVED GERM CELLS IN THE RECIPIENT GONAD. IN THIS STUDY, A GIANT GOURAMI (OSPHRONEMUS GORAMY) VASA-LIKE GENE (GGVLG) WAS CLONED AND CHARACTERIZED FOR USE AS A MOLECULAR MARKER FOR GERM CELLS IN THIS SPECIES. NUCLEOTIDE SEQUENCE ANALYSIS REVEALED THAT GGVLG COMPRISES 2,340 BPS WITH AN OPEN READING FRAME OF 1,962 BPS ENCODING 653 AMINO ACIDS. THE DEDUCED AMINO ACID SEQUENCE CONTAINED 17 ARGININE- ALIMUDDIN, .; ANDRIYANI, IRMA; JUNIOR, MUHAMMAD ZAIRIN; ARFAH, HARTON; OCTAVERA, ANNA; CARMAN, ODANG; YOSHIZAKI, GORO
HAYATI Journal of Biosciences Vol. 18 No. 3 (2011): September 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.3.135

Abstract

Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.
MORPHOLOGICAL CHARACTERISTIC OF SPERMATOGONIA AND TESTES DISSOCIATION : A Preliminary Study for the Germ Cell Transplantation in Giant Gouramy (Osphronemus gouramy) Andriani, Irma; Djuwita, Ita; Sumantadinata, Komar; Junior, Muhammad Zairin; Arfah, Harton; Alimuddin, Alimuddin
Indonesian Aquaculture Journal Vol 5, No 2 (2010): (December 2010)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.767 KB) | DOI: 10.15578/iaj.5.2.2010.163-172

Abstract

The recent study were attempting to develop spermatogonial germ cell transplantation as a tool to preserve and propagate male germ-plasm from endangered fish species, as well as to produce surrogate broodstock of commercially valuable fish. Spermatogonia identification and testes dissociation were the first necessary steps to obtain highly amount and viable population of spermatogonia as donor cells for transplantation. Using giant gouramy testes as a model, spermatogonia was histological characterized and two methods of testes dissociations were compared (i.e. medium A contained 0.5% trypsin in PBS and medium B contained 0.5% trypsin and DNase 10 IU/μL in PBS complemented with CaCl2, Hepes and FCS). Optimal incubation times (1, 2, 3, 4 and 5 hours) in dissociation medium were also determined. Freshly isolated testes of immature giant gouramy were minced in dissociation medium and then incubated to get monodisperce cell suspension. Parameters observed were number and viability of spermatogonia (ø > 10 μm). The viability was analyzed using trypan blue exclusion dye. The results showed that the average number of spermatogonia observed in medium B was higher than in medium A (P<0.05), meanwhile the viability of spermatogonia between medium A and B were not significantly different (P>0.05). The viability of spermatogonia decreased by the increasing duration time of dissociation. The viability of spermatogonia started to decrease significantly in 2 hours incubation time in medium A and 4 hours incubation time in medium B (P<0.05). In conclusion, application of dissociation medium B yielded higher number of viable spermatogonia than dissociation medium A.
PENGARUH PEMBERIAN HORMON ITe METILTESTOSTERON SECARA ORAL PADA INDI.,K IKAN GAPI (Poecilia retidlau Pcters) STRAIN TUXEDO TERHADAP JENIS KELAMIN KETURUNANNYA Arma, Nur Rachmawaty; Mulyati, Lilis; Sumantadinata, Komar; Jr, Muhammad Zairin; Arfah, Harton
Jurnal Penelitian Perikanan Indonesia Vol 1, No 3 (1995): (Vol.1 No.3 1995)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (789.28 KB) | DOI: 10.15578/jppi.1.3.1995.74-81

Abstract

Percobaan dilakukan untuk mengetahui pengaruh pemberian hormon lzo-metiltestosteron secara oral pada induk ikan gapi (Poecilia reticulau Peters) strain tuxedo terhadap ienis kelamin keturunannya.
SUPPLEMENTATION OF ASTAXANTHIN AND VITAMIN E IN FEED ON THE DEVELOPMENT OF GONADS WHITE SHRIMP BROODSTOCK LITOPENAEUS VANNAMEI BOONE 1931 Maulana, Fajar; Arfah, Harton; Istifarini, Mita; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3478.477 KB) | DOI: 10.19027/jai.16.2.124-135

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ABSTRACT The quality of white shrimp Litopenaeus vannamei broodstock can be improved through the addition of astaxanthin and vitamin E in the diet. This study aimed to determine the effect of administration of astaxanthin and vitamin E with different doses in the feed on the maturity gonad of prospective Pacific white shrimp broodstock. Supplementation of 0 mg/kg feed astaxanthin + 0 mg/kg feed vitamin E (control/A),  500 mg/kg feed astaxanthin (B),  350 mg/kg  feed vitamin E (C), 500 mg/kg feed astaxanthin and 350 mg/kg feed vitamin E (D), and  250 mg/kg feed astaxanthin and 175 mg/kg feed vitamin E (E) were applied in feed formulation. Shrimp was fed 2% of body weight three times daily at 06.00 am, 13.00 pm, and 20.00 pm. The result showed that the optimum dose for survival, specific growth rate and maturity level of Pacific white shrimp broodstock was obtained in the combination of 175 mg/kg vitamin E and 250 mg/kg astaxanthin. The  survival of shrimp by that treatment was 100.00±0.00%, specific growth rate 1.07±0.26%/day, the first level of gonad maturity growth was reached at day 14 (19.45±4.81%), the fourth level of gonad maturity was obtained at day 41, spawning rate 33.33±8.33%, fecundity 87,000±2,000 eggs, and hatching rate reached 49.00±1.53%. Keywords: astaxanthin, Litopenaeus vannamei, vitamin E  ABSTRAK Peningkatan kualitas induk udang vaname Litopenaeus vannamei dapat dilakukan dengan penambahan vitamin E dan astaxanthin pada pakan. Tujuan penelitian ini adalah untuk mengevaluasi pengaruh pemberian astaxanthin dan vitamin E dengan dosis berbeda dalam pakan terhadap tingkat kematangan gonad calon induk udang vaname. Dosis yang digunakan adalah 0 mg/kg pakan astaxanthin + 0 mg/kg pakan vitamin E (kontrol/A),  500 mg/kg pakan astaxanthin (B),  350 mg/kg  pakan vitamin E (C), 500 mg/kg pakan astaxanthin and 350 mg/kg pakan vitamin E (D), and  250 mg/kg pakan astaxanthin and 175 mg/kg pakan vitamin E (E). Pemberian pakan dengan penambahan vitamin E dan astaxanthin dilakukan sebanyak tiga kali, yaitu jam 06.00, 13.00, dan 20.00 WIB sebanyak 2% dari bobot udang. Hasil penelitian menunjukkan bahwa dosis optimum untuk sintasan, laju pertumbuhan spesifik, dan tingkat kematangan induk udang vaname diperoleh dengan kombinasi 175 mg/kg vitamin E dan 250 mg/kg astaxanthin. Kelangsungan hidup udang dengan perlakuan tersebut adalah 100,00±0,00%, laju pertumbuhan spesifik 1,07±0,26%/hari, tingkat kematangan gonad pertama dicapai pada hari ke 14 (19,45±4,81%), tingkat kematangan gonad keempat diperoleh pada hari ke 41, tingkat pemijahan 33,33±8,33%, fekunditas 87.000±2.000 telur, dan tingkat penetasan mencapai 49,00±1,53%.  Kata kunci: astaxanthin, udang vaname Litopenaeus vannamei, vitamin E
Ekspresi gen aromatase pada pengarahan diferensiasi kelamin ikan nila (Oreochromis niloticus Linnaeus 1758) menggunakan madu [Aromatase gene expression of sex reversal Nile tilapia (Oreochromis niloticus Linnaeus 1758) using honey] Heriyati, Eny; Alimuddin, nFN; Arfah, Harton; Sudrajat, Agus Oman
Jurnal Iktiologi Indonesia Vol 15, No 1 (2015): Februari 2015
Publisher : Masyarakat Iktiologi Indonesia (MII )

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.035 KB) | DOI: 10.32491/jii.v15i1.74

Abstract

In tilapia aquaculture, all male populations are preferred because they achieve higher growth rates and prevent uncontrolled reproduction. Sex reversal techniques are largely used for the control of sex in fish farming and in fundamental studies on sex determinism mechanisms. The study was conducted to determine the effect of immersion Nile tilapia larvae in water containing different honey source on male percentage and aromatase gene expression. In experiment I, a total of 30 tilapia larvae at 12 days post hatch were immersed in water containing honey derived from the forest, cultured and mangrove bees, at a dose of 10 ml L-1 for 10 hours. Fish were maintained in the same condition for two months. The results showed that percentage of male fish was similar among honey treatments (p>0.05), and they were significantly different with the control (p<0.05). In experiment II, fish were immersed in two bioactive compounds of honey, namely chrysin and potassium solution in a dose of 20 mg L-1 and 0.026 g L-1, respectively, to verify the bioactive affects sex differentiation. Aroma-g expression was analyzed by RT-PCR method. Tissue was collected at 1, 6, 12, 24 and 48 hours after immersion, and 2-month-old fish. Size fragment DNA aroma-g of female 200 bp. Chrysin and potassium immersion increased male percentage (p<0.1), this indicated that both materials were involved in Nile tilapia sex differentiation. RT-PCR analysis showed that honey, chrysin and potassium down-regulated aroma-g expression at 12 hours post immersion. Thus, honey can be used for sex reverse of Nile tilapia, and the mechanism is most likely as aromatase inhibitors. AbstrakBudi daya ikan nila dengan populasi jantan semua (monoseks) lebih memberikan keuntungan karena laju pertumbuhan-nya lebih cepat dan dapat mencegah pemijahan liar.Teknik pengarahan diferensiasi kelamin(sex reversal) digunakan untuk mengarahkan pembentukan jenis kelamin pada budi daya ikan.Penelitian ini dilakukan untuk mengevaluasi pe-ngaruh perendaman larva ikan nila menggunakan tiga sumber madu berbeda terhadap persentase ikan jantan dan ekspresi gen aromatase. Pada percobaan satu, 30 larva ikan nila berumur 12 hari setelah menetas direndam menggunakan madu hutan, madu ternak dan madu bakau, dengan dosis 10 ml L-1 air selama 10 jam. Ikan dipelihara dalam kondisi yang sama selama dua bulan. Hasil penelitian menunjukkan bahwa persentase ikan jantan tidak berbeda nyata antar perlakuan madu (p>0,05), tetapi semuanya berbeda nyata dengan kontrol (p<0,05). Pada percobaan kedua, larva ikan nila direndam dalam air mengandung dua bahan bioaktif madu, yakni chrysin dan kalium dengan dosis masing-masing 20 mg L-1 dan 0,026 g L-1. Ekspresi gen aromatase tipe gonad (aroma-g) dan tipe otak (aroma-o) dianalisis menggunakan metode RT-PCR. Sampel jaringan diambil pada waktu 1, 6, 12, 24, dan 48 jam pascaperlakuan madu, chrysin, dan kalium, serta setelah ikan berumur dua bulan.Ukuran fragmen DNA aromatase pada gonad betina sekitar 200 bp. Perendaman chrysin dan kalium meningkatkan persentase ikan jantan (p<0,1). Analisis RT-PCR menunjukkan bahwa madu, chrysin, dan kalium dapat menekan ekspresi gen aroma-g pada jam ke-12 pascaperendaman. Dengan demikian dapat disimpulkan bahwa madu, chrysin dan kalium dapat digunakan untuk pengarahan diferensiasi ikan nila, dan mekanis-menya seperti penghambat aromatase.