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PENGARUH PERBEDAAN PADAT TEBAR LARVA TERHADAP PERTUMBUHAN DAN SINTASAN PADA IKAN UCENG (NEMACHEILUS FASCIATUS) Subagja, Jojo; Prakoso, Vitas Atmadi; Arifin, Otong Zenal; Kristanto, Anang Hari
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3561

Abstract

Proper stocking density on larval rearing of barred loach (Nemacheilus fasciatus Valenciennes, 1846) is very important in terms of obtaining the best growth and survival rate. This study was aimed to observe the growth and survival rate of barred loach subjected to different larval stocking density. Fish larvae were obtained by artificial spawning from in aquarium. Larvae were stocked on aquariums (50 × 40 × 30 cm) with three stocking density treatments (5, 10, and 15 individuals/L) and three replications. The experimental fish larvae were fed by Artemia and commercial feed (crude protein content: 30%) and feeding frequency twice per day (ad libitum). The length and weight were reassured every 10 days during 70 days of rearing period. Temperature, pH, and dissolved oxygen were measured at each treatment. Data obtained from measurements were analysed to assess the length gain, weight gain, specific growth rate (SGR), biomass gain, and survival rate. The results showed length gain of 3.35 ± 0.01, 2.94 ± 0.10, and 2.82 ± 0.15 cm and weight gain of 0.573 ± 0.023, 0.436 ± 0.018, and 0.417 ± 0.043 g in larval stocking density of 5, 10 and 15 individuals/L, respectively. Specific growth rates and biomass growth were of 6.22 ± 0.41, 5.93 ± 0.21, and 5.68 ± 0.41 %/day and 94.04 ± 5.39, 146.80 ± 3.81, and 167.24 ± 17.48 g in larval stocking density of 5, 10 and 15 individuals/L, respectively. Meanwhile, the survival rates obtained in this study were 89.13 ± 7.25, 93.13 ± 5.13, and 76.50 ± 3.54 % in larval stocking density of 5, 10 and 15 individuals/L, respectively. Increased stocking density has a negative impact on the growth and survival rate of barred loach.
KERAGAMAN GENETIK HlBRIDA BEBERAPA STRAIN IKAN NILA {Oreochromis niloticus Bleeker) Gustiano, Rudhy; Soelistyowati, Dinar; Fauzan, Agung Luthfi; Arifin, Otong Zenal
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.839 KB) | DOI: 10.14203/beritabiologi.v10i6.1951

Abstract

Objectives of this study are to determine the genetic variability among progeny of intraspecific crossing between three strains of Oreochromis niloticus Bleeker (BEST, Nirwana, and Red NIFI) using Randomly Amplified Polymorphic DNA (RAPD) and truss morphometric. The results showed that hybridization enabled to increase genetic variability respectedly on progeny of Nirwana male crossed to BEST female (27,93%) as well as to Red NIFI female. An analysist of truss morphometric indicated that BEST population had the lowest similarity and largest genetic distance (0,6) to the Nirwana and Red NIFI which were closer to the hybrid populations.
STUDI BIOMETRI BERDASARKAN MERISTIK DAN MORFOMETRIK IKAN GURAMI GALUR BASTAR DAN BLUESAFIR [Biometrical Study Based on Meristic and Morphometric of Giant Gouramy Strain Bastar and Bluesafir] Radona, Deni; Nafiqoh, Nunak; Arifin, Otong Zenal; Arifin, Otong Zenal
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3222.906 KB) | DOI: 10.14203/beritabiologi.v16i2.2758

Abstract

Identification of Gouramy is required to obtain phenotype characteristics information of the population for sustainable aquaculture  practices. In this study phenotype diversity of giant gouramy strain bastar and bluesafir was analyzed based on  meristic and morphometric measurement. Sample, are taken from 30 individuals of each population. The result showed that there were differences in colour patterns between the two populations, bastar population had yellowish brown body and red fins, whilst bluesafir population had brown blackish brown body and fins. The canonical function of truss morphometric analysis on 21 characteristics showed the measurement distribution of that both populations were in different quadrants. The intrapopulation similarity index showed that population of strain bluesafir had higher genetic similarity (80%) with interpopulation similarity index of 23.3%, while the intrapopulation similarity index of strain bastar was 76.7%.
STUDI BIOMETRI BERDASARKAN MERISTIK DAN MORFOMETRIK IKAN GURAMI GALUR BASTAR DAN BLUESAFIR [Biometrical Study Based on Meristic and Morphometric of Giant Gouramy Strain Bastar and Bluesafir] Radona, Deni; Nafiqoh, Nunak; Arifin, Otong Zenal; Arifin, Otong Zenal
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3222.906 KB) | DOI: 10.14203/beritabiologi.v16i2.2758

Abstract

Identification of Gouramy is required to obtain phenotype characteristics information of the population for sustainable aquaculture  practices. In this study phenotype diversity of giant gouramy strain bastar and bluesafir was analyzed based on  meristic and morphometric measurement. Sample, are taken from 30 individuals of each population. The result showed that there were differences in colour patterns between the two populations, bastar population had yellowish brown body and red fins, whilst bluesafir population had brown blackish brown body and fins. The canonical function of truss morphometric analysis on 21 characteristics showed the measurement distribution of that both populations were in different quadrants. The intrapopulation similarity index showed that population of strain bluesafir had higher genetic similarity (80%) with interpopulation similarity index of 23.3%, while the intrapopulation similarity index of strain bastar was 76.7%.
Evaluasi Pertumbuhan Empat Populasi Ikan Nila (Oreochromis niloticus) di Kolam Percobaan Cijeruk, Bogor Gustiano, Rudhy; Kurniasih, Titin; Arifin, Otong Zenal
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 3 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.3.177

Abstract

The objective of the present study was to obtain good populations of Oreochromis niloticus based on their growth performances. Four populations, i.e GIFT-6, Tempe, Local, and GIFT-3 were used in this study. Sixteen 16 cages laid in four earthen ponds in the Cijeruk Research Installation, Bogor, were used as replication. The results showed that there were no significant difference among four populations for biomass, but a significant difference among populations was observed on length parameter (F < 0.01),in which the local population was the best.
Feminisasi Nila (GIFT), Oreochromis sp. Menggunakan Hormon Estradiol 17-β Kurniasih, Titin; Arifin, Otong Zenal; Marizal, Marizal
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.166

Abstract

Research to find out the effect of oral administration of estradiol 17-β  on feminization of Oreochromis sp. (GIFT) fry was carried out at Cibalagung Research Station. Five different concentrations of estradiol 17-β  i.e.: 60, 80, 100, 120 and 0 mg/kg feed, were given to larvae of 6 days old twice a day for 21 days in triplicates. The result indicated that there was significant difference of average percentage of female between control and treatments.The highest percentage of female was obtained from fish treated with 100 mg estradiol /kg feed (86.6%), whereas the lowest was obtained from fish control (51.7%). The survival rate and length growth were not significantly different. Survival rate of larvae ranged from 94 to 97.7% and the average of length growth ranged from 10.08 to 13.32 mm.
KERAGAAN PERTUMBUHAN IKAN TAMBAKAN (HELOSTOMA TEMMINCKII CUVIER, 1829) DENGAN KEPADATAN BERBEDA Arifin, Otong Zenal; Cahyanti, Wahyulia; Prakoso, Vitas Atmadi
Media Akuakultur Vol 14, No 2 (2019): (Desember, 2019)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.14.2.2019.83-87

Abstract

Padat tebar merupakan salah satu faktor yang berpengaruh terhadap keragaan pertumbuhan dalam budidaya ikan. Penelitian ini dilakukan untuk mengetahui kepadatan optimum untuk pertumbuhan ikan tambakan. Penelitian ini dilakukan di Instalasi Riset Plasma Nutfah Perikanan Air Tawar, Cijeruk, Balai Riset Perikanan Budidaya Air Tawar dan Penyuluhan Perikanan (BRPBATPP), Bogor. Ikan tambakan generasi ke-2 dengan panjang total 2,10 ± 0,18 cm dan bobot 4,83 ± 0,17 g yang diperoleh melalui program domestikasi dipelihara di kolam bak beton yang diberi sekat (ukuran 3 m x 3,5 m x 1 m). Pakan komersial berupa pelet terapung (protein 39%-41%; lemak 5%) diberikan sebanyak 3% per hari dari biomassa dengan frekuensi pemberian sebanyak tiga kali. Perlakuan kepadatan yang dilakukan pada penelitian adalah 25, 50, dan 75 ekor/m2 dengan tiga ulangan. Pengambilan data dilakukan setiap 30 hari sebanyak 30 ekor dari setiap ulangan selama 150 hari masa pemeliharaan terhadap panjang, bobot, kelangsungan hidup, biomassa, dan rasio konversi pakan. Pengukuran kualitas air juga dilakukan sebagai data pendukung. Hasil penelitian menunjukkan bahwa pertumbuhan panjang baku, panjang total, bobot, dan sintasan tertinggi diperoleh pada kepadatan 25 ekor/m2.Stocking density is one of the influencing growth performance indicators in aquaculture. This study was conducted to determine the optimum stocking density in optimizing the growth rate of kissing gourami. The study was carried out at the Research Station for Freshwater Fisheries Germplasm, Cijeruk, RIFAFE Bogor. A second generation of kissing gourami with total length of 2.10 ± 0.18 cm and body weight of 4.83 ± 0.17 g produced from a domestication program were reared in separated-nets concrete ponds (pond division: 3 m x 3,5 m x 1 m) and fed using commercial floating pellets (protein: 39%-41%; lipid: 5%). Feed was given 3% of biomass per day with a feeding frequency of three times per day. Stocking density treatments in this study were 25, 50, and 75 fish/m2 with three replications. Data collection was conducted every 30 days from 30 fish in each replicate during 150 days of rearing period on growth parameters including length, weight, survival rate, biomass, and feed conversion ratio. Water quality measurement was also conducted as supplementary data. The results showed that the highest values on standard length gain, absolute length gain, weight gain, and survival rate were found in the fish population with a stocking density of 25 fish/m2.
KERAGAMAN GENETIK POPULASI IKAN NILA {Oreochromis niloticus) DALAM PROGRAM SELEKSI BERDASARKAN RAPD Arifin, Otong Zenal; Nugroho, Estu; Gustiano, Rudhy
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (442.087 KB) | DOI: 10.14203/beritabiologi.v8i6.826

Abstract

Objectives of the study was to discover genetic variability and genetic relationship of paternal half sib population of nile tilapia (Oreochromis niloticus) under Selection Program Scheme at Research Institute for Freshwater Aquaculture, in Bogor, West Java.Four populations from unrelated selected breeders were observed for genetic variability using RAPD. The analysis of amplification from each locus and fragments were used to estimate DNA polymorphisms, heterozygosity, fst and genetic distance.The range of heterozygosity of four examined populations was 0.1760-0.2168 with level polymorphic between 47.66% and 64.86%.The highest heterozygosity and polymorphic was on population 1 and the lowest one was on population 2. Fst test showed significance among the populations. The closest relationship was between population 1 and 4 (0.1978) and the furthest was population 2 and 3 (0.3289).
ANALISIS KERAGAMAN GENETIK TIGA STRAIN NILA MERAH (Oreochromis sp) DENGAN ANOVA RAPD Iskandariah, Iskandariah; Arifin, Otong Zenal; Gustiano, Rudhy
Jurnal Sains Natural Vol 1, No 1 (2011): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.626 KB) | DOI: 10.31938/jsn.v1i1.8

Abstract

Analysis Genetic Variation of Three Strains of Red Tilapia by Anova of RAPD.          Study on the genetic variance of three strains of red tilapia (Oreochromis sp) had been conducted in the Biology Molecular Laboratory, Research Institute for Freshwater Aquaculture (RIFA) Bogor.  Three different strains, Red NIFI from Thailand,  Red Tilapia from Lido lake and Red Tilapia from Bogor?s farmer were analyzed in the study.  Observation used Random Amplified Polymorphism DNA (RAPD) with OPA-03, OPA-04, OPC 14 and OPC-15 primers.  The results showed that only OPA-03 primer was able to amplify numerous samples.  Further analysis showed that the percentage of polimorphic range was between 16.67 ? 38.89%, heterozygosity value 0.0378 ? 0.1536 and genetic distance among strain 0.3051 ? 0.6037.Keywords : RAPD, genetic, strain, nile tilapia, oreochromis ABSTRAK           Penelitian mengenai variasi genetik tiga strain nila merah dari 3 lokasi yang berbeda telah dilakukan di Laboratorium Molekuler Biologi, Balai Riset Perikanan Budidaya Air Tawar (BRPBAT) Bogor.  Strain yang diamati meliputi jenis nila Red NIFI dari Thailand, nila merah dari Danau Lido dan nila merah dari Petani Bogor. Penelitian menggunakan metode analisis Random Amplified Polymorphism DNA (RAPD), dengan menggunakan primer OPA-03, OPA-04, OPC-14 dan OPC-15.  Hasil pengamatan menunjukkan hanya OPA-03 yang dapat menghasilkan amplifikasi dalam jumlah sampel yang memadai.  Hasil analisis menunjukkan bahwa persentase polimorfik berkisar antara 20.00-40.00%, dengan nilai heterozigositas 0.0604?0.1516 dan jarak genetik antar strain 0.1770-0.4865.  Kata kunci : RAPD, genetik, strain, ikan nila, oreochromis
PRODUKSI IKAN UNGGUL DI LAHAN MINAPADI SECARA INTENSIF Cahyanti, Wahyulia; Prakoso, Vitas Atmadi; Arifin, Otong Zenal; Kusmini, Irin Iriana
Jurnal Sains Natural Vol 4, No 1 (2014): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.811 KB) | DOI: 10.31938/jsn.v4i1.72

Abstract

Superior Fish Production in Intensive Paddy Cum Fish Culture         Aquaculture development in Indonesia is expected to push forward the fish farmers entrepreneurship and competitiveness of aquaculture products in a sustainable manner through improved efficiency and cooperative advantages. Development of fisheries resources in accordance with the management and the optimal use or to provide added value of the fish itself also to provide other products. One technology that can be used is through technology in paddy cum fish culture. This study aimed to improve of land productivity through superior tilapia production in paddy cum fish culture system. In general, the best results obtained in treatment A (no treatment). Productivity of rice and fish could be enhanced by applying paddy cum fish culture  system by 4 to 5.5 million or 10-15% per hectare rice field.Keywords: Minapadi, Productivity, Nila, Fertilizer ABSTRAK          Pengembangan perikanan budidaya di Indonesia ke depan diharapkan dapat  mendorong masyarakat perikanan/pembudidaya untuk meningkatkan jiwa kewirausahaan dan daya saing produk perikanan budidaya secara berkelanjutan melalui peningkatan efisiensi dan keunggulan koperatif. Pengembangan sumber daya perikanan yang sesuai dengan pengelolaan dan pemanfaatan yang optimal dan dapat memberikan nilai tambah selain dari ikannya itu sendiri juga produk lainnya. Salah satu teknologi yang dapat digunakan yaitu melalui teknologi minapadi. Penelitian ini bertujuan untukmeningkatkan produktivitas lahan melalui produksi ikan nila unggul dalam sistem minapadi. secara umum, hasil terbaik diperoleh pada perlakuan A (tanpa perlakuan). Dari hasil penelitian, didapatkan kesimpulan bahwa Ikan nila lebih baik pertumbuhannya dibanding mas.Secara umum hasil terbaik diperoleh dari lahan kontrol atau tanpa perlakuan. Produktivitas padi dan ikan dapat ditingkatkan dengan menerapkan sistem minapadi senilai 4-5,5 juta atau 10-15% tiap hektar sawah.Kata kunci : minapadi, produktifitas, Nila, pupuk