Teuku Armansyah
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Published : 13 Documents
Articles

Found 13 Documents
Search

TAMPILAN REPRODUKSI KAMBING BETINA LOKAL YANG INDUKSI BERAHINYA DILAKUKAN DENGAN SISTEM SINKRONISASI SINGKAT Nizwan Siregar, Tongku; Armansyah, Teuku; Sayuti, Arman; -, Syafruddin
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this study is to determine the reproduction performance of local does in which theirestrous was induced by short synchronization system. In this study 10 healthy female, unpregnant andhave relatively homogenous body weights were used. All does were devided into 2 experiment groups.Group 1 consisted of 4 does as control and group II consisted of 6 does as treatment. Group I, were injectedwith 125 ?g cloprostenol intramuscular, twice in 11 days interval. Group II, was treated with shortsynchronization using 125 ?g cloprostenol intramuscular, and then followed by injection of 300 IU hCGand 0.5 mg estradiol benzoate after 12 hours later. The does were inseminated 10 hours after onset ofestrous and repeated 12 hours later. Parameters measured were oestrous percentage, pregnancy, andlitter size. Results showed that all does (100%) from both groups showed estrous. Percentage of pregnantdoes group I and II were 75.00% and 83.33% respectively, and the averages litter size were 2.0 ± 1.0 and 1.4± 0.3, respetively. Treatment with short synchronization can increase pregnancy and delivered percentagealthough litter size not affected.
MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (PENGUKURAN TESTOSTERON SERUM KAMBING KACANG DENGAN TEKNIK ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA): PENTINGNYA VALIDASI KIT) G, Gholib; Wahyuni, Sri; Kadar, Okta Hilda; Adam, Mulyadi; Lubis, Triva Murtina; A, Azhar; Akmal, Muslim; Siregar, Tongku Nizwan; Armansyah, Teuku; Nugraha, Taufiq Purna
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3367

Abstract

This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) inanalytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks ( 2 years), six kids ( 6 months), and six does ( 2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.65±4.27%, 10%, 15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P 0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat
THE IMAGE OF EMBRYO AND FETAL OF ETAWA CROSSBRED GOATS USING ULTRASONOGRAPHY AT DIFFERENT GESTATIONAL AGES Melia, Juli; Fauziah, Fauziah; Hamdan, Hamdan; Panjaitan, Budianto; Syafruddin, Syafruddin; Armansyah, Teuku; Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.3768

Abstract

This study aimed to estimate the gestational age of Etawa crossbred goats based on ultrasound images of embryo and fetal usingtranscutaneous ultrasonography. Etawa crossbred does (n= 5) were synchronized using double PGF2? injection with 10 days interval and matednaturally. Early pregnancy was diagnosed on 20th day after mating base on isoechogenic image of the embryo (2.65±0.75 mm) andhypoechogenic appearance of gestational sac. On 30th day of the first trimester, the embryo size was 8.31±0.42 mm. The embryo reached10.44±0.51 mm on 35th day. On 60th day, the existence of the fetal were clearly appeared along with isoechogenic of umbilical cord,hyperechogenic os cranium (25.5±0.1 mm), and body length (51.83±1.59 mm). On 75th day, the diameter of the os cranium was 33.03±0.20 mm,body length of 110±1.73 mm, and bones structures appeared hyperechogenic for os thoracalis and os vertebrae. The placenta was isoechogenoicwith diameter of 23.3±0.2 mm. On 90th day, the placenta dominated the uterus (22.67±1.70 mm) and reached its maximum diameter on 145th day(33.73±2.91 mm). In conclusion, early pregnancy detection of Etawa crossbred goats using transcutaneous ultrasonography could be performedon 20th day after mating
PERBANDINGAN INTENSITAS BERAHI SAPI ACEH YANG DISINKRONISASI DENGAN PROSTAGLANDIN F2 ALFA DAN BERAHI ALAMI Hafizuddin, Hafizuddin; Siregar, Tongku Nizwan; Akmal, Muslim; Melia, Juli; rizal, Husnur; Armansyah, Teuku
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.296

Abstract

Penelitian ini bertujuan mengetahui perbedaan intensitas berahi sapi aceh antara yang disinkronisasi berahi dengan prostaglandin F2 alfa (PGF2?) dan berahi alami. Dalam penelitian ini digunakan 20 ekor sapi aceh betina yang dibagi atas dua kelompok. Kriteria sapi yang digunakan adalah umur 5-8 tahun, mempunyai bobot badan 150-250 kg, dan mempunyai minimal dua siklus reguler. Sapi yang digunakan mempunyai skor kondisi tubuh dengan kriteria baik, yaitu 3 atau 4 pada skala skor 5. Pada Kelompok I (KI) sapi disinkronisasi berahi mengunakan PGF2? sebanyak 5 mg/ml secara intramuskular. Pada kelompok II (KII) sapi dibiarkan memperlihatkan gejala berahi alami. Penilaian intensitas berahi dilakukan dengan memberi skor 1, 2, dan 3, berdasarkan kriteria yang dibuat oleh Kune dan Solihati (2007). Hasil penelitian menunjukkan tidak ada perbedaan intensitas berahi sapi aceh baik yang disinkronisasi berahi dengan PGF2? dan sapi yang mengalami berahi alami dengan skor intensitas berahi masing-masing adalah 2,40±0,84 dan 2,70±0,48.
LEVEL STEROID SAPI ACEH YANG DIINDUKSI DENGAN PREGNANT MARE’S SERUM GONADOTROPIN (PMSG) DAN FOLLICLE STIMULATING HORMONE (FSH) A, Amiruddin; Siregar, Tongku Nizwan; Armansyah, Teuku; H, Hamdan; munandar, Aris; Rifki, Muhammad
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.923

Abstract

Penelitian ini bertujuan mengetahui pengaruh induksi superovulasi dengan pregnant mare?s serum gonadothropin (PMSG) dan follicle stimulating hormone (FSH) terhadap peningkatan level steroid sapi aceh. Penelitian ini menggunakan 6 ekor sapi aceh betina dengan status tidak bunting, minimal 2 bulan pasca partus, sudah pernah beranak, dan sehat secara klinis. Sapi dibagi atas dua kelompok, masing-masing 3 ekor untuk tiap kelompok. Pada kelompok I, sapi diinjeksi dengan 1.500 IU PMSG pada hari ke-9 yang diikuti dengan penyuntikan 5 ml prostaglandin pada hari ke-11. Pada kelompok II, hari ke-9 sampai hari ke-12, sapi diinjeksi dengan FSH dua kali sehari (pagi dan sore, 08.00 dan 16.00 WIB) menggunakan dosis bertingkat yakni 3-3, 2-2, 1-1, dan 0,5-0,5 ml. Pada hari ke-11 sapi diinjeksi dengan 2,5 ml prostaglandin (pagi dan sore, 08.00 dan 16.00 WIB). Koleksi darah untuk pemeriksaan estrogen dilakukan ketika sapi memperlihatkan gejala berahi (saat inseminasi) setelah pemberian PMSG dan FSH yang diikuti dengan pemberian prostaglandin (berahi sesudah superovulasi) sedangkan koleksi darah untuk pemeriksaan konsentrasi progesteron dilakukan pada hari ke-7 setelah inseminasi. Pengukuran konsentrasi estrogen dan progesteron dilakukan dengan metode enzymelinkedimmunosorbanassay (ELISA). Konsentrasi estrogen pada saat estrus setelah induksi superovulasi dengan PMSG dan FSH masing-masing adalah 89,46±2,46 dan 54,62+9,91 pg/ml sedangkan konsentrasi progesteron pada hari ke-7 setelah inseminasi masing-masing adalah 14,78±2,33 dan 17,40±5,8 ng/ml. Hormon PMSG mempunyai kemampuan yang lebih baik dalam meningkatkan konsentrasi estrogen pada saat berahi tetapi hormon FSH mempunyai kemampuan yang lebih baik dibandingkan dengan hormon PMSG dalam meningkatkan konsentrasi progesteron hari ke-7 setelah inseminasi.
ACEH CATTLE FOLLICLE DYNAMIC UNDER ENVIRONMENTAL HEAT STRESS Armansyah, Teuku; Siregar, Tongku Nizwan; Aliza, Dwinna; Melia, Juli; Meutia, Nellita; Panjaitan, Budianto; Hafizuddin, Hafizuddin; Adam, Mulyadi; Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.6377

Abstract

The objective of this research is to know Aceh cattle follicle dynamic under environmental heat stress condition. This research was conducted on December 2012 until August 2013. Animals used in this research were 20 Aceh cattle aged 5-8 years old, weighing of 150-250 kg, and had at least 2 regular cycles. All cattle used were clinically in good body condition score. The cattle were divided into two groups of 10, used in two different period of times (December-January and July-August) to examine the effect of environmental heat to their follicle dynamic. Each group is separated into two different keeping management; one group was kept in pens while the others in the pasture, 5 cattle in each management group. Research timing was based on information acquired from Indrapuri Agency for Meteorology, Climatology, and Geophysics (BMKG) which predicted that extreme weather would last from July to August while December to January would be relatively normal in Aceh region. All cattle?s estrous cycle were synchronized by 5 mg/mL of PGF2? intramuscularly (Lutalyse?, Pharmacia Upjohn Company, Pfizer Inc.). Ultrasonography examination was performed to monitor ovary follicle?s growth and dynamic during one cycle. Days during ovulation marked by standing heat was regarded as Day 0 of estrous cycle. Follicular dynamic examination during estrous cycle on December-January and also on July-August, both penned or pastured cattle showed the follicle waves that was 3 follicle growth waves. The size of follicle growth on first wave (1st DF) on cattle kept in pen on July-August and December-January were relatively similar. The size of follicle growth on first wave for cattle kept in pasture on July-August and December-January were relatively similar. To conclude, Aceh cattle?s follicle dynamic is not change during environment heat stress condition and is not affected by different cattle-keeping management.
The Potency of Sernai (Wedelia biflora) Leaf Water Extract as Antipyretic Compared to Amino Fenol Derivate and Salicylic Acid on Mice (Mus Musculus) Rinidar, Rinidar; Armansyah, Teuku; Putri, Tesha Aprilya
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.479 KB) | DOI: 10.21157/j.med.vet..v8i2.3358

Abstract

The study was conducted to screen the antipyretic activity of aqueous of extract of the leaves of sernai (Wedelia biflora) with split-plot design. Eighteen healthy mice weighing between (20-25 g), aged 2-3 months were divided into six groups of three animals each: negative control group were given distilled water (P0), P1, and P2 as a positive control respectively given salicylic acid 42.25 mg/kg body weight, the amino phenol 25 mg/kg body weight, group P3, P4, and P5 given of sernai leaf aqueous extract at a concentration of 50, 75, and 100% respectively. The initial rectal temperature of each animal was recorded by digital thermometer. The pyrexia was induced by injecting a solution of 12.5% peptone. The difference in temperature was observed every 30 minutes for 3 hours and respective time interval was found out by statistical method. The results showed that the water extract of leaves sernai with various concentrations significantly different (P<0.01) with an observation time. Average temperatures in the P5 mice was significantly different (P<0.05) with P0. P3 and P4 is not significantly different from P0, but P3 and P4 significantly different (P<0.05) with P2 and not significantly different from P1. P5 was not significantly different from the P2 and P1 significantly different. It was concluded that the aqueous extract of leaves sernai potential as an antipyretic in mice and at concentrations of 50 and 75% effective in reducing fever in mice tend equivalent to the amino phenol, while the concentration of 100% with salicylic acid.Key words: amino fenol, antipyretic, salicylic acid, sernai
ANALISIS ISOZIM UNTUK MENGETAHUI VARIASI GENETIK SEBAGAI UPAYA PEMURNIAN RAS SAPI ACEH Armansyah, Teuku; -, Al-Azhar; Siregar, Tongku Nizwan
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study was performed to investigate genetic variation of Aceh?s cattle based on analysis of malatedehydrogenase (MDH) and esterase isozymes. Blood samples were collected from 30 out of 345 Aceh?scattles raised at the Center for Breeding Prime Animals (BPTU) of Indrapuri, Aceh Besar. The phenotypecharacteristics of each cattle were recorded before blood collection, and then analyzed based on theRegulation of Agricultural Minister of Indonesia No. 54/permentan/ot.140/10/2006 whereas isozymesanalysis was done by vertical acrylamide gel electrophoresis under constant current of 10 mA at 4°C for 4hours and appropriate staining systems. The pattern band which showed the genotype of the testedcattles, were then used as the basis to determinate the heterozygosity and proportion of polymorphic locus.The results showed that phenotype variation was found among Aceh?s cattle raised at the BPTU Indrapuri,Aceh Besar. Two locus detected in this research were MDH 1 and MDH2, whereas esterase locus (Est1 andEst2) were not detected due to the unsuccesful of the electrophoresis. MDH1 and MDH2 loci in the redblood cells of Aceh?s cattle were polymorphic. On the basis of malate dehydrogenase isozymes usingMVSP 3.1 Program and Simple Matching Coefficient it could be concluded that there was individualgenetic variation among Aceh?s cattles raised at the BPTU Indrapuri, Aceh Besar.
DAYA LARVASIDA EKSTRAK ETIL ASETAT DAUN KEMUNING (Murraya paniculata (L) Jack) TERHADAP LARVA NYAMUK Aedes aegypti Minarni, Emi; Armansyah, Teuku; Hanafiah, Muhammad
Jurnal Medika Veterinaria Vol 7, No 1 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.691 KB) | DOI: 10.21157/j.med.vet..v7i1.2915

Abstract

Penelitian ini bertujuan mengetahui efektivitas larvasida ekstrak etil asetat daun kemuning terhadap larva Ae. aegypti. Materi yang digunakan dalam penelitian ini adalah ekstrak etil asetat daun kemuning (EEADK). Rancangan penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri atas enam perlakuan yaitu P1 (100 ml aquades), P2 (abate 10%), P3 (EEADK 10 ppm), P4 (EEADK 50 ppm), P5 (EEADK 100 ppm), dan P6 (EEADK 1000 ppm). Pada masing-masing perlakuan ditambahkan 25 larva nyamuk Ae. Aegypti dan ragi sebagai makanan. Masing-masing perlakuan diulang sebanyak 3 kali ulangan. Hasil yang diperoleh dianalisis dengan menggunakan analisis varian (ANAVA). Rata-rata jumlah larva yang mati 24 jam setelah pemberian ekstrak pada kelompok P1; P2; P3; P4; P5; dan P6 masing-masing adalah 25,00±0,00; 0,00±0,00; 0,33±0,39; 1,00±0,00 ; 2,33±0,94; dan 3,33±0,47. Berdasarkan analisis uji Puncan menunjukkan bahwa terdapat perbedaan yang nyata (P<0,05) antara kelompok perlakuan dengan kelompok kontrol. Pemberian ekstrak etil asetat daun kemuning dapat menurunkan jumlah larva Ae. Aegypti.
PENINGKATAN AKTIVITAS LUTEOLITIK SETELAH PEMBERIAN EKSTRAK VESIKULA SEMINALIS SAPI PADA TIKUS PUTIH (RATTUS NORVEGICUS) r, Rahmandi; Siregar, Tongku Nizwan; Akmal, Muslim; Armansyah, Teuku; s, Syafruddin
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.576

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak vesikula seminalis terhadap penurunan konsentrasi progesteron serta diameter korpus luteum pada tikus putih. Dalam penelitian ini digunakan 30 ekor tikus putih (Rattus norvegicus) betina dewasa, galur Wistar, berumur 3-4 bulan dengan berat badan antara 200-250 g dan dibagi atas dua kelompok (K1 dan K2) masing-masing diberi 25 ?g cloprostenol dan 0,2 cc ekstrak vesikula seminalis secara intravaginal pada hari ke-7 kebuntingan. Tiga ekor tikus masing-masing kelompok dibunuh pada jam ke-0, 3, 6, 12, dan 26. Pemeriksaan progesteron dilakukan menggunakan metode enzymelinkedimmunosorbantassay (ELISA). Konsentrasi progesteron pada kelompok perlakuan PGF2? dan ekstrak vesikula seminalis pada lima periode waktu pengukuran yakni jam ke-0, 3, 6, 12, dan 26 memperlihatkan perbedaan yang signifikan (P 0,05). Ekstrak vesikula seminalis mempunyai kemampuan yang sama dengan PGF2? komersial dalam menurunkan diameter korpus luteum yang ditandai secara mikroskopis dengan berkurangnya vaskularisasi darah menuju ovarium (P 0,05). Disimpulkan bahwa ekstrak vesikula seminalis mempunyai kemampuan yang lebih baik dibandingkan dengan PGF2? komersial dalam menurunkan konsentrasi progesteron tikus putih dan mempunyai kemampuan yang sama dengan PGF2? komersial dalam menurunkan diameter CL.