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ENHANCEMENT OF COLOUR QUALITY, GROWTH, AND HEALTH STATUS OF RAINBOW KURUMOI FISH MELANOTAENIA PARVA THROUGH DIETARY SYNTHETIC CAROTENOIDS SUPPLEMENTATION Meilisza, Nina; Suprayudi, Muhammad Agus; Jusadi, Dedi; Junior, Muhammad Zairin; Artika, I Made; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3529.969 KB) | DOI: 10.19027/jai.18.1.54-69

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ABSTRACT Carotenoids were known as pigment sources, the precursor of vitamin A, potential antioxidant and can improve the health status of fish. Furthermore, there are also studies that reveal the role of carotenoids in bone formation and metabolism. This study was conducted to determine the effect of different carotenoids at two different doses in the feed on growth, pigmentation, vitamin A conversion, blood profile, antioxidant activity, and calcium bone of the rainbow fish. Twenty-one aquariums with a volume of 20 L each stocked with 20 fish (1.08 ± 0.03 g of body weight and 4.56 ± 0.07 cm of body length). The experiment applied seven experimental diets (in triplicates) consisted of three types of carotenoids were astaxanthin (AS), canthaxanthin (CS), and lutein (LS) and two doses (130 and 260 mg/kg), i.e. AS-130, AS-260, CS-130, CS-260, LS-130, LS-260 and basal (without carotenoids) as the control. The fish were fed for 56 days of experimental period. The results showed that carotenoid diets were able to increase growth, total carotenoids, percentages of chromatophores, vitamin A conversion, erythrocyte, leukocytes, packed cell volume (PCV), neutrophils, and hemoglobin (Hb) compared to the control. Fish fed dietary astaxanthin at a level of 260 mg/kg was superior compared to other diets. Dietary carotenoids were also capable of decreasing the endogenous antioxidant activity of superoxide dismutase (SOD) and malonyl dialdehyde (MDA) and increased the calcium level in fish bone than basal diet. Keywords: carotenoids, growth, health status, Melanotaenia parva, pigmentation  ABSTRAK Karotenoid diketahui sebagai sumber pigmen, prekursor vitamin A, antioksidan potensial dan dapat meningkatkan status kesehatan ikan. Selain itu, karotenoid juga memiliki peran dalam formasi dan metabolism tulang. Penelitian ini dilakukan untuk mengevaluasi pengaruh jenis dan dosis karotenoid yang berbeda terhadap pertumbuhan, pigmentasi, konversi vitamin A, gambaran darah, aktifitas antioksidan dan kalsium tulang ikan rainbow Kurumoi. Sebanyak 20 ekor ikan (bobot tubuh rata-rata 1,08 ± 0,03 g dan panjang total rata-rata 4,56 ± 0,07 cm) dan diberi makan pakan yang mengandung karotenoid. Pakan uji terdiri atas tiga jenis karotenoid dengan tiga ulangan yaitu astaksantin (AS), cantaksantin (CS), dan lutein (LS) dan dua dosis (130 dan 260 mg/kg) dikodekan dengan AS-130, AS-260, CS-130, CS-260, LS-130, LS-260 dan basal (tanpa karotenoid) sebagai kontrol. Ikan diberi makan selama 56 hari pemeliharaan. Hasil penelitian menunjukkan bahwa karotenoid dapat meningkatkan pertumbuhan, total karotenoid, persentase kromatofora, konversi vitamin A, eritrosit, leukosit, hematokrit, neutrofil, dan hemoglobin dibandingkan dengan kontrol. Ikan yang diberi pakan mengandung astaksantin 200 mg/kg lebih baik dibandingkan denga pakan uji lainnya. Pakan yang mengandung karotenoid juga mampu menurunkan antioksidan endogenus superoxide dismutase (SOD) dan malonyl dialdehyde (MDA), serta meningkatkan kalsium tulang ikan dibandingkan pakan kontrol. Keywords: Melanotaenia parva, karotenoid, pertumbuhan, pigmentasi, status kesehatan. 
PENERAPAN PENDEKATAN READER RESPONS DALAM MENINGKATKAN KEMAMPUAN MEMBACA NOVEL SISWA SMP MUTIARA SINGARAJA Harsana, Ngurah; Artika, I Made; Sudiana, I Nyoman
Jurnal Pendidikan Bahasa dan Sastra Indonesia Undiksha Vol 8, No 1 (2018)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpbs.v8i1.20590

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Penelitian tindakan kelas yang dilakukan di SMP Mutiara Singaraja ini bertujuan (1) mengidentifikasi langkah-langkah penerapan pendekatan reader respons dalam meningkatkan kemampuan membaca novel siswa SMP Mutiara Singaraja, (2) mengetahui peningkatan kemampuan membaca novel siswa dengan menggunakan pendekatan reader respons di SMP Mutiara Singaraja, dan (3) mengetahui respons siswa terhadap penerapan pendekatan reader respons dalam pembelajaran memahami buku novel. Penelitian ini dilaksanakan dengan dua siklus dan menggunakan lima tahap penelitian tindakan kelas, yakni (1) refleksi awal, (2) perencanaan tindakan, (3) pelaksanaan, (4) observasi dan evaluasi, dan (5) refleksi. Data dikumpulkan dengan metode observasi, tes, angket/kuisioner, serta wawancara dan dianalisis secara deskriptif kuantitatif dan kualitatif. Hasil penelitian menunjukkan nilai rata-rata siklus I adalah 69,05 atau mengalami peningkatan 8,35% dari nilai sebelumnya 60,70. Nilai rata-rata siklus II adalah 79,85 atau meningkat sebesar 10,8% dari siklus I dan meningkat 19,15% dari nilai rata-rata prasiklus. Perolehan rata-rata respons siswa pada siklus I sebesar 43,05 dalam kategori positif dan pada siklus II mengalami peningkatan sebesar 1,6%, sehingga menjadi 44,65 dalam kategori positif.
OPTIMASI PRODUKSI ENZIMATIS DIASILGLISEROL MELALUI GLISEROLISIS KONTINU [OPTIMIZATION OF ENZYMATIC DIACYLGLYCEROL PRODUCTION THROUGH CONTINUOUS GLYCEROLYSIS] Tri-Panji, -; Palilingan, Septiany C.; Artika, I Made
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.346 KB) | DOI: 10.6066/jtip.2014.25.1.16

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OPTIMASI PRODUKSI ENZIMATIS DIASILGLISEROL MELALUI GLISEROLISIS KONTINU[Optimization of Enzymatic Diacylglycerol Production through Continuous Glycerolysis]Tri-Panji1)*, Septiany C. Palilingan2) dan I Made Artika2)1) Balai Penelitian Bioteknologi Perkebunan Indonesia, Jl. Taman Kencana No. 1 Bogor2) Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor Diterima 24 Juni 2013 / Disetujui 10 Februari 2014ABSTRACT  Diacylglycerol (DAG) produced from crude palm oil (CPO) is one of the healthy oils that can be consumed for daily human diet. DAG production in Indonesia is constrained by the high cost of the mostly imported lipase. To overcome this problem, research of DAG production has been carried out using crude extracts of lipase produced by local species of fungi Rhizopus oryzae. This study aims to develop a continuous process of enzymatic glycerolysis of CPO for DAG production; to establish optimum conditions of DAG production which includes flow rate of CPO and glycerolysis time; and to test the performance of lipase from the local mold R. oryzae in catalyzing continuous process of glycerolysis for the production of DAG. Lipase isolation was carried out by acetone precipitation and lipase was used as a catalyst in the continuous glycerolysis process. The glycerolysis was conducted by reacting CPO with glycerol continuously at various time periods. The optimum condition of automatic continuous glycerolysis process was achieved at a CPO flow rate of 3 mL/min with a glycerolysis time at the 18 cycles (9 hours). The conversion of DAG was 29%. The performance of lipase was proven to remain stable up to 3 times changes of CPO substrate for 9 hours of glycerolysis process with the best condition at the 3 cycles and can improved conversion of DAG until 37%.
PENERAPAN TEKNOLOGI NANOPARTIKEL UNTUK SEDIAAN OBAT (ANTIBIOTIK BERBASIS BAHAN ALAM, PROPOLIS TRIGONA SPP) Hasan, H. A. E. Zainal; Artika, I Made; Fahri, Vita Rosaline; Sari, Nurmala
CHEMISTRY PROGRESS Vol 5, No 1 (2012)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.5.1.2012.655

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Zainal Hasan dkk., 2012. Penerapan teknologi nanopartikel untuk sediaan obat (antibiotik berbasis bahanalam, Propolis Trigona spp.). Propolis Trigona spp telah diteliti dapat menghambat pertumbuhan bakteri patogen seperti Escherichia coli.Senyawa Flavonoid yang terkandung dalam propolis diduga berperan sebagai antibakteri. Jika ukuran partikelmakin kecil maka luas permukaan partikel makin besar sehingga laju dari larutan semakin meningkat danmempercepat penyerapan obat melalui peredaran darah sehingga efek terapeutiknya lebih cepat tercapai.Salah satu cara yang dapat dilakukan untuk memperkecil ukuran partikel guna meningkatkan kelarutan danpenyerapan suatu sediaan farmasi adalah dengan menggunakan nanoteknologi.Pada penelitian ini telah dirancang sediaan propolis dalam bentuk nanopartikel. Tujuan penelitian ini adalahmembuat nanopartikel dari propolis Trigona spp. Proses pembuatan nanopropolis yaitu dengan cara penyalutandan homogenizer pada kecepatan tinggi. Telah dilakukan uji aktivitas dari nanopartikel propolis sebagaiantibakteri dan menentukan konsentrasi hambat tumbuh minimum propolis nanopartikel terhadap bakteriEscherichia coli secara in vitro. Dari hasil penelitian ini menunjukkan bahwa propolis Trigona spp dapat dibuatdalam bentuk nanopartikel. Hasil Scanning Electron Microscopy menunjukkan adanya ukuran diameternanopropolis sebesar 100 ? 322 nm. Hasil uji aktivitas antibakteri menunjukkan pada konsentrasi 10% - 0,02%nanopropolis aktif terhadap E. coli sedangkan propolis bukan nanopartikel aktif sampai konsentrasi 0,15%.Dengan demikian KHTM ?propolis bukan nanopartikel? dicapai pada konsentrasi 0,15%, sedangkannanopropolis KHTM nya lebih rendah dari 0,02%. Propolis nanopartikel lebih efektif dibandingkan ?propolisbukan nanopartikel? dalam menghambat pertumbuhan E. coli.Kata kunci : propolis, nanopartikel, E.coli, KHTMZainal Hasan et al., 2011. Application of nanoparticle technology for medicine preparate (Antibiotic base onnatural product, Propolis Trigona spp.). Trigona spp propolis has been evaluated can inhibit Escherichia coli. Flavonoid in propolis suggested act asantibacterial. If particle size smaller, then its surface area is bigger, so the rate of drugs absorption wereincreased trough blood circulation and its therapy effect can be achieved faster. One of resize way to reduceparticle size to increase its solubility dan absorption pharmaceutical is using nanotechnology.This research has been designed propolis in nanoparticle size. The aims of this research is to makenanoparticle propolis from Trigona spp. The process to make nanopropolis was covered it and homogenize inhigh speed. Nanopropolis activity evaluation has been done as antibacterial and determined its minimuminhibition concentration on Escherichia coli (in vitro). Trigona spp propolis can be made to nanoparticle. ElectronMicroscopy scanning shows nanopropolis diameter was 100 ? 322 nm. Antibacterial activity evaluation showson nanoproposis concentration 10% - 0,02% active on E. Coli, although nonnanopropolis active onconcentration 0,15%. Nanopropolis is more effective than non nanopropolis to inhibit E. coli.Keywords : propolis, nanopartikel, E.coli, KHTM
Antibacterial Activity and Phytochemical Analysis of Geranium homeanum Turez Leaves Rahmawati, Fri; Bintang, Maria; Artika, I Made
Current Biochemistry Vol 4, No 3 (2017)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/13-22

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Geranium homeanum Turez is a herbaceous plant used as an empirical medicine. This research was carried out to test the antibacterial activity and determine minimum inhibition concentration (MIC) to Staphylococcus aureus and Pseudomonas aeruginosa with agar diffution method and phytochemical analysis of geranium leaves  by Harbone method. The young and old geranium leaves were blended then filtrated. The obtained filtrate was divided into two parts, one part heated by autoclave and the other was unheated. Each filtrate was tested against to Staphylococcus aureus and Pseudomonas aeruginosa. Geranium leaves filtrate having the highest antibacterial activity was heated at 500C until it  become dry powder.  The powder was used to measure MIC and phytochemical analysis.The results showed that the antibacterial activity of young leaves filtrate was higher than the old leaves filtrat, and the unheated leaves filtrate was higher than heated filtrate one. MIC of S. aureus and P. aeruginosa, were as follows 15 mg/ml and 20 mg/mL respectively. The antibacterial activity of powder geranium’s leaves  filtrate was weaker than 100 µg/mL ampicilline. Phytochemical analysis of geranium leaves showed positive contents of alkaloid and triterpenoid.
AKTIVITAS KITINASE DAN PEROKSIDASE DARI EKSTRAK PROTEIN DAUN, AKAR, KALUS DAN TUNAS IN VITRO TRICHOSANTHES TRICUSPIDATA LOUR. Sukma, Dewi; Poerwanto, Roedhy; Sudarsono, ,; Khumaida, Nurul; Wiyono, Suryo; Artika, I Made
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.991 KB) | DOI: 10.24831/jai.v36i1.1347

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A number of Trichosanthes species has been reported as a source of bioactive protein associated with defense mechanisms such as chitinase. Chitinase and peroxidase of crude protein extracted from leaves, roots, in vitro calli and shoots of T. tricuspidata had been analysed. Calli were induced on MS medium containing combinations of 1 µM NAA + 1 µM BA  (K1), 2 µM NAA + 2 µM BA (K2), 3 µM NAA + 3 µM BA (K3), or 4 µM NAA + 4 µM BA (K4). Shoots were cultured in MS with 1 mg/l of BA, while leaves and roots were harvested from six-month old plants grown on the field. Results of the experiment suggested that K1-K4 medium could be used to induce calli although weight of calli from all medium composition  was not significantly different (0.19-0.31 g/explant/4 weeks). Calli from K1 medium had the highest of total crude protein content (3.24 mg/ml). The highest of chitinase activity was found in in vitro shoots (6.51 mM pNP/hour/mg protein) and the highest peroxidase activity was in the plant roots (0.25 ? 420/minute/mg protein).   Key words:  in vitro calli, shoots, crude protein, chitinase and peroxidase activities
Isolation and Selection of Thermophilic Bacteria as Hexavalent Chromium Reducer from Batik Processing Waste Water Wijiastuti, Wijiastuti; Artika, I Made; Nurhidayat, Novik
Current Biochemistry Vol 2, No 1 (2015)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/22-31

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Hexavalent chromium (Cr VI) in the oxidized state is carcinogenic in humans. Chromium is widely used in various industries, and therefore Cr (VI) can be found in wastes from the industry. Heavy metal Cr (VI) waste, is one type of hazardous wastes, due to the high toxicity of Cr (VI) which is much higher than that of Cr (III). This study was conducted to isolate naturally occurring bacteria from batik wastewater with ability to reduce Cr (VI). Identification of chromate reductase gene was carried out using qPCR method. Results showed that three isolates Bacillus sp.1a, Pseudomonas sp.1b, dan Geobacillus sp.1c have chromate reductase-coding genes. Based on the results of qPCR analysis, the isolate Bacillus sp.1a was predicted to have the highest reduction activity. Hence, this isolate was then subjected to Cr(VI) reduction activity test.
Trametes versicolor as Agent for Delignification of Rice Husks Nurjanah, Laita; Falah, Syamsul; Azhari, Azmi; Suryani, Suryani; Artika, I Made
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

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the earth for the production of biofuels and other valuable products. It is comprised of the carbohydratepolymers, cellulose, hemicellulose, and an aromatic polymer, lignin. One of the methods for removing thelignin component of rice husks is by delignification using white-rot-fungi. The aim of the study was to carry outdelignification of rice husks using white-rot-fungi. The white-rot-fungi used here were Trametes versicolor andPhanerochaete chrysosporium. The study consisted of a biomass and microbial preparation, chemical assay ofthe rice husk, ligninase enzyme tests, and delignification of rice husks. Results showed that T. versicolor and P.chrysosporium have ligninase enzyme. The precentage of lignin from the total biomass rice husks was 23.61%w/w, and following the delignification process by T. versicolor for 20 days, the remaining lignin was 16.20%w/w, making the percentage of rice husks lignin degraded as 7.41% w/w. The biodelignification process alsodecreased the percentage of holocellullose, cellulose, and other extracted substances, and accordingly thisincreased the percentage of hemicellulose. Based on the ability of T. versicolor to degrade lignin of the ricehusk at room temperature (28ºC) as mentioned above, it can be concluded that T. versicolor has potential tobe used for delignification process
Isolation and Molecular Cloning of Cellulase Gene from Bovine Rumen Bacteria Pratama, Rahadian; Artika, I Made; Chaidamsari, Tetty; Sugiarti, Herti; Putra, Soekarno Mismana
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

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Cellulases are the enzymes that hydrolyze cellulosic biomass and are produced by the microorganismsthat grow over cellulosic matters. The objective of this research was to isolate and clone cellulasegene from cellulose-degrading bacteria of bovine rumen. Cellulose-degrading bacteria was isolated fromrumen fluid using a selective medium. Total RNA was isolated from selected colony having cellulose degradingactivity and was used as a template for cDNA construction using reverse transcriptase polymerasechain reaction (RT-PCR) technique. The resulted cDNA was employed as a template for PCR amplificationof cellulase gene using specific primers. The cellulase gene candidate obtained was cloned intothe pGEM-T-Easy vector followed by determination of its nucleotide sequence. The sequence was thenaligned with sequences of cellulase genes from GenBank. Results showed that a number of isolates of rumenbacteria exhibit cellulase activity and the CR-8 isolate was selected for further analysis. The successfulisolation of total RNA from CR-8 was indicated by the presence of two intense bands of ribosomal RNA(23S and 16S). The reverse transcription process was successful and the amplification of cellulase geneusing the specific primers F1 and R1 resulted in a DNA fragment of 1900 bp as a candidate of cellulasegene. The fragment was successfully cloned into the pGEM-T-Easy vector, and the resulted recombinantplasmid was successfully introduced into the E. coli cells. Nucleotide sequence analysis suggested that thecloned gene is cellulase gene and shares 99% homology with the endo-1,6-beta-glucanase of T. harzianum.
BIOFLOCCULANT PRODUCING-BACTERIA FROM TAPIOCA WASTE WATER WERE CHARACTERIZED. TWO BACTERIAL ISOLATES I.E. LT-5 AND LT-6 ISOLATES HAD HIGH FLOCCULATION ACTIVITY, WITH ACTIVITY OF 68.92 AND 71.38% RESPECTIVELY. THE FLOCCULATION ACTIVITY OF LT-5 ISOLATE INCREASED AT PH 2.0-4.0 (ACIDIC CONDITION), HOWEVER THE ACTIVITY OF LT-6 INCREASED AT PH 6.0-8.0 (NEUTRAL). ADDITION OF 0.05% OF ALCL3 AS CATION WAS THE MOST EFFECTIVE AND HAD IMPORTANT ROLE IN FLOCCULATION ACTIVITY. BASED ON THE MORPHOLOGICAL PROPE SURYANI, .; AMBARSARI, LAKSMI; ARTIKA, I MADE; SUSANTI, HARTUTIK EKA
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.193

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Bioflocculant producing-bacteria from tapioca waste water were characterized. Two bacterial isolates i.e. LT-5 and LT-6 isolates had high flocculation activity, with activity of 68.92 and 71.38% respectively. The flocculation activity of LT-5 isolate increased at pH 2.0-4.0 (acidic condition), however the activity of LT-6 increased at pH 6.0-8.0 (neutral). Addition of 0.05% of AlCl3 as cation was the most effective and had important role in flocculation activity. Based on the morphological properties, LT-5 isolate was identified as Chromobacterium violaceum and LT-6 isolate was identified as Citrobacter koseri.
Co-Authors , Sudarsono - Tri-Panji . SURYANI A. E. Zainal Hasan Abdul Choliq Abidin, Syaeful Agung Eru Wibowo AHMAD ENDANG ZAINAL HASAN Akhmad Endang Zainal Hasan Amanda, Nisa Widya Amanda, Nisa Widya AMIN FATONI Antonius Padua Ratu, Antonius Padua Apon Zaenal Mustopa Aprianti, Apipah Arendra, Arya Arian, Perkasa Azmi Azhari, Azmi Dedi Jusadi Denny Irawati Desi Purwaningsih DEWI SUKMA DIMAS ANDRIANTO DIMAWARNITA, Firda DWI N. SUSILOWATI Erismar Amri Evi Nur Qolbaini Febrianti, Fina H. A. E. Zainal Hasan Hami Seno, Djarot Sasongko HANIF, Hyakansa Harsana, Ngurah Hartono, Lusiana Kresnawati HARTUTIK EKA SUSANTI HASIM DANURI Herti Sugiarti, Herti I Nyoman Sudiana Ifa Manzila Iman Rusmana Inawati Inawati, Inawati K, Popi A Khusniati, Tatik kurnia agustini Kuswandi Kuswandi Laita Nurjanah, Laita LAKSMI AMBARSARI Lasmiyanti, Metty MARIA BINTANG Meilisza, Nina Mirza Dikari Kusrini MS, Yulia Atika MUHAMMAD AGUS SUPRAYUDI Muhammad Dailami, Muhammad MUHAMMAD ZAIRIN JUNIOR Norman Razief Azwar Novik Nurhidayat NUNUK WIDHYASTUTI Nur Bambang Priyo Utomo Nur Hasanah Nurmala Sari Nurul Khumaida PANJI, TRI - PUJI LESTARI Puspasari, Erna Putu Suwardike Rahadian Pratama, Rahadian Rahmawati, Fri Rahmi, Hayatul ROEDHY POERWANTO Safari, Dodi Septiany C. Palilingan Sheryn Sunni Albani Soekarno Mismana Putra, Soekarno Mismana SOGANDI, SOGANDI SUHARYANTO SUHARYANTO Suhyana, Jajang Sulfianti, Asri Sulistiani Sulistiani, Sulistiani Suryani Suryani SURYO WIYONO Sutoro Sutoro Syamsul Falah Tetty Chaidamsari, Tetty Tri Panji Trini Suryani Kadir Vita Rosaline Fahri Wahyuni, Putu Sri Waras Nurcholis Wasrin Syafii Wijiastuti, Wijiastuti Yadi Suryadi YUNITA ARIAN SANI ANWAR