Articles

Found 17 Documents
Search

ANALISIS KANDUNGAN FENOLIK EKSTRAK DAUN JATI (TECTONA GRANDIS L.) DENGAN WAKTU DEKOMPOSISI YANG BERBEDA Astiti, Ni Putu Adriani
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p18

Abstract

Teak (Tectona grandis L.) is a plant with strong wood and is resistant to pests and diseases. In addition, its leaf extract is widely used by public as an additive in foods both as a preservative and as an alternative food coloring. The objective of this research was to determine phenolic compounds of teak leaves extracted with three different decomposition times (one, two and three weeks of decomposition). Extraction was conducted by using maceration method in which water was used as solvent. Identification of Phenolics was done by using Gas Chromatography. The results showed that the compounds of vanilic acid, salicylic acid, ferulic acid, cumaric acid, gallic acid, benzoic acid and cafeic acid were identified in the analysis. The concentration and composition of phenolic compounds in the extract was determined by decomposition.
EFEKTIVITAS EKSTRAK KULIT BATANG AMPUPU (EUCALYPTUS ALBA REINW. EX. BLUME) DALAM MENGHAMBAT PERTUMBUHAN JAMUR FUSARIUM SP. PENYEBAB BUSUK TONGKOL JAGUNG (ZEA MAYS L.) Metboki, Bernadina; Astiti, Ni Putu Adriani; Proborini, Meitini Wahyuni
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i02.p01

Abstract

The fungal-related diseases were responsible as one of many reasons for low production of maize in Indonesia. Controlling fungal contaminants using chemical is hazardous for human nowadays. Thus the need of environmental friendly vegetative-based fungicide such as from the extract of Ampupu tree bark is important. The objective of this study was to investigate the antifungal effect from Ampupu tree bark crude extract in response to growth of Fusarium sp, which responsible as the causal agent of corncob rot. The inhibition test on growth of Fusarium sp. by Ampupu tree bark crude extract was performed with diffusion well method on PDA as media. The process involved the infusion of crude extracts at 1.5%, 3.0% and 4.5% concentration, respectively, into diffusion well on each Petri disk. The result showed that by in vivo treatment, crude extract of Ampupu tree bark with concentration of 1.5%, 3,0% and 4.5%, were able to inhibit the fungal growth of Fusarium miniliforme as the causal agent of corncob rot with diameter of inhibition zone 0.18 mm, 1.85 mm and 2.01 mm, respectively. The higher the concentration of the crude extract given, the larger the diameter of inhibition zone formed.
PEMBERIAN KOLKHISIN DENGAN LAMA PERENDAMAN BERBEDA PADA INDUKSI POLIPLOIDI TANAMAN PACAR AIR (IMPATIENS BALSAMINA L.) Wiendra, Ni Made Sastriyani; Pharmawati, Made; Astiti, Ni Putu Adriani
Jurnal Biologi Udayana Vol 15 No 1 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this research was to induce polyploidy on Impatiens balsamina L. Seedlings of I. balsamina was immersed in 0,01% colchicines solution for 4, 6, 8, and 24 hours. Chromosome of I. balsamina was visualized using squash method stained with aceto-orcein. Morphological observations were conducted on plant height, stem circumference, leaf length and width, number of branches flowering time and size of flower. The result revealed that 0,01% solution of colchicine was able to induce polyploidy on I. balsamina. Obsevation on morphological characteristic showed that colchicine treatment increased plant height, stem circumference, leaf length and number of branches. Colchicine treatment induced earlier flowering time, but flower size was unaffected. Twelve chromosomes (2n = 2x = 12) were observed in the diploid seedlings while 25 chromosomes were observed in the tetraploid (2n = 4x = 24 seedlings.
DAYA HAMBAT EKSTRAK DAUN RAMBUTAN RAPIAH (NEPHELIUM LAPPACEUM L.) TERHADAP PERTUMBUHAN TANAMAN TEMULAWAK Dharmadewi, A. A. Istri Mirah; Astiti, Ni Putu Adriani; Wrasiati, Luh Putu
Jurnal Biologi Udayana Vol 18 No 2 (2014): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rambutan rapiah (Nephelium lappaceum L.) merupakan salah satu tanaman yang dapat digunakan sebagai obat yang termasuk kedalam family Sapindaceae. Rambutan mengandung senyawa alelopati yang dapat menghambat pertumbuhan tanaman yang tumbuh disekitarnya. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun rambutan rapiah terhadap pertumbuhan temulawak serta mengetahui kemampuan tanaman temulawak untuk bertahan terhadap pemberian ekstrak daun rambutan. Parameter yang diamati adalah tinggi tanaman, panjang daun, lebar daun, jumlah daun, jumlah tunas dan berat rimpang yang diamati pada akhir pengamatan. Pemberian ekstrak daun rambutan dengan konsentrasi 5%,10%,15%, 20% mengakibatkan tinggi tanaman, panjang daun, lebardaun, jumlah daun dan berat rimpang lebih rendah jika dibandingkan dengan kontrol.
ELUSIDASI AWAL DAYA HAMBAT EKSTRAK ETANOL DAUN KETAPANG (TERMINALIA CATAPPA L.) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS ATCC25923 PENYEBAB GINGIVITIS Purwaningsih, Putu Pande; Darmayasa, Ida Bagus; Astiti, Ni Putu Adriani
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p08

Abstract

Daun Ketapang (Terminalia catappa L.) dapat dimanfaatkan sebagai obat tradisional. Penggunaan bahan alam sebagai obat secara umum dinilai lebih aman serta memiliki efek samping yang relatif lebih kecil dibandingkan penggunaan obat modern. Penelitian ini bertujuan untuk mengetahui elusidasi awal daya hambat ekstrak etanol daun ketapang terhadap pertumbuhan Staphylococcus aureus ATCC25923 penyebab gingivitis,dan untuk mengetahui senyawa aktif yang terdapat pada Daun Ketapang.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan konsentrasi ekstrak daun ketapang yaitu 1%, 2%,3%, 4%, 5%, kontrol positif (chlorexidine) dan kontrol negatif (etanol). Penentuan daya hambat ekstrak daun ketapang terhadap bakteri S. aureus menggunakan metode Kirby Beuer ditandai dengan terbentuknya zona bening akibat pemberian senyawa antibakteri yang berdifusi pada media tumbuh bakteri. Hasil Penelitian menunjukkan bahwa semakin besar pemberian konsentrasi ekstrak (5%) maka semakin besar zona hambat yang terbentuk.Nilai efektivitas antibakteri daun ketapang tidak lebih baik daripada antiseptik yang diujikan. Hasil uji profil fitokimia menunjukkan bahwa ekstrak daun ketapang mengandung tannin, saponin, terpenoid dan flavonoid. Hasil uji GC-MS teridentifikasi lima senyawa diantaranya yaitu Alpha Terpinolene, Cyclohexanol 5-methyl, Hexadecanoic acid, Octadecanoic acid, 1.2Benzenedicarboxylic acid. Kelima senyawa aktif tersebut berpotensi sebagai antibakteri.
PERBANYAKAN ANGGREK DENDROBIUM HETEROCARPUM LINDL. SECARA IN VITRO DENGAN MEDIA YANG BERBEDA Setiawati, Yuli; Astarini, Ida Ayu; Astiti, Ni Putu Adriani
Metamorfosa: Journal of Biological Sciences Vol 2 No 1 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Dendrobium heterocarpum Lindl. is one of the orchid species which is often seeked. It is because this species has a mystique appearance and special fragrance. The research aimed to observe the growth and developing responses of protocorm like bodies (PLBs) of D. heterocarpum after 12 weeks in three different medium, i.e. Murashige and Skoog (MS), Kursor C and Western 3 (W3). The D. heterocarpum seeds were planted in MS, Kursor C and W3 medium. Qualitatively the orchid growth were observe including growth score and colour score. The result showed that growing time of MS was 4 weeks, a week faster than the growing time on Kursor C and W3 medium. The growing phase on MS medium and Kursor C medium reached phase 1 whereas on W3 medium reached phase 2. PLB?s colour after 12 weeks on MS medium reached phase E, strong yellowish green phase (142A). Meanwhile, on Kursor C and W3 was on phase D, moderate yellowish green (143D).
KANDUNGAN FITOKIMIA EKSTRAK DAUN KAMBOJA (PLUMERIA SP.) DAN PENGARUHNYA TERHADAP PERTUMBUHAN TANAMAN JAHE EMPRIT (ZINGIBER OFFICINALE VAR. AMARUM) Budaya, Putu Yayun Antari; Astiti, Ni Putu Adriani; Kriswiyanti, Eniek
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tanaman kamboja mengandung senyawa alelopati yang menghambat pertumbuhan tanaman di sekitarnya.  Penelitian ini bertujuan untuk mengetahui kandungan fitokimia ekstrak daun kamboja dan pengaruhnya terhadap pertumbuhan tanaman jahe emprit (Zingiber officinale var. amarum). Penelitian dilaksanakan dalam dua tahap yaitu, tahap pertama uji fitokimia ekstrak daun kamboja merah dan daun kamboja putih, serta tahap kedua berupa uji respon pertumbuhan tanaman jahe emprit yang diberi perlakuan ekstrak daun kamboja. Variabel respon pertumbuhan yang diamati adalah jumlah tunas tumbuh, tinggi tanaman, jumlah daun, jumlah akar, berat rimpang, berat basah berangkasan, dan berat kering berangkasan. Hasil uji fitokimia menunjukkan bahwa pada ekstrak daun kamboja putih dan kamboja merah memiliki jenis golongan senyawa aktif yang sama yaitu triterpenoid, steroid, flavonoid, dan polifenol dalam intensitas yang berbeda. Ekstrak daun kamboja putih memberikan efekdaya hambat yang lebih kuat dibandingkan dengan ekstrak daun kamboja merah. Persentase daya hambat ekstrak daun kamboja putih terhadap tinggi tanaman jahe emprit sebesar 80,5%, berat basah berangkasan 64%, jumlah daun 54%, berat kering berangkasan 54%, jumlah tunas tumbuh 33,3%, dan berat rimpang 31,9%.
UJI AKTIVITAS EKSTRAK REBUNG BAMBU TABAH (GIGANTOCHLOA NIGROCILIATA BUSE-KURZ) TERHADAP PERILAKU KAWIN MENCIT JANTAN (MUS MUSCULUS L.) Padmiswari, A. A. Istri Mas; Sukmaningsih K, A. A. S. A; Astiti, Ni Putu Adriani
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitan ini bertujuan untuk mengetahui pengaruh ekstrak rebung bambu tabah terhadap perilaku kawin mencit jantan. Rancangan yang digunakan pada penelitian ini adalah rancangan eksperimental sederhana dengan4 kelompok perlakuan dan 6 ulangan, yaitu P0: Kontrol (Perlakuan  dengan pemberian CMC Na 0,5 %), P1: (Perlakuan dengan pemberian ekstrak 200 mg/kg bb), P2: (Perlakuan dengan pemberian ekstrak 300 mg/kg bb) dan P3: (Perlakuan dengan pemberian ekstrak 400 mg/kg). Perlakuan ekstrak rebung bambu tabah diberikan pada mencit jantan secara oral setiap hari selama 33 hari dan pengamatan perilaku kawin dilakukan 3 hari sekali. Variabel yang diamati untuk menilai perilaku kawin mencit jantan adalah jumlah mount. Data dianalisa dengan menggunakan uji One Way Annova. Hasil penelitian menunjukkan bahwa pemberian ekstrak rebung bambu tabah berpengaruh signifikan terhadap peningkatan perilaku kawin mencit jantan (P<0,05).
EFEKTIFITAS DAYA HAMBAT BAKTERI STREPTOMYCES SP TERHADAP ERWINIA SP PENYEBAB PENYAKIT BUSUK REBAH PADA TANAMAN LIDAH BUAYA (ALOE BARBADENSIS MILL) TASNIM, SARMILA; KAWURI, RETNO; ASTITI, NI PUTU ADRIANI
SIMBIOSIS Vol I, No 1, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Streptomyces sp was conducted from December 2010 - June 2011 at the Laboratoryof Microbiology, Biology Department, Math and Science Faculty, UdayanaUniversity Bukit Jimbaran-Bali. Implementation stages of the research consisted ofisolation and testing of the antibiotic activity Streptomyces sp to inhibit growthbacterial pathogens Erwinia sp as a cause of disease in plants fallen foul (Soft rot) ofAloe barbadensis Mill.The results of this study have eight isolates of Streptomyces spwith macroscopic and microscopic characters are varied. Furthermore, all isolateswere obtained and then tested against antibiotic activity to inhibit growth the bacteriaErwinia sp. Test results obtained by Streptomyces sp that has the most effective ininhibiting the ability of the bacteria Erwinia sp isolates are Streptomyces sp2for (45%).
IN VITRO CALLUS INDUCTION OF ‘SHIRAZ’ GRAPE (VITIS VINIFERA L.) USING DIFFERENT MEDIUM AND GROWTH REGULATOR COMBINATION Arieswari, Ni Nyoman Nila; Astarini, Ida Ayu; Astiti, Ni Putu Adriani; Pramana, Jeremy
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2018.v06.i01.p04

Abstract

One of grape cultivars widely used as raw material for wine production is ?Shiraz? cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ?Shiraz? callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ?Shiraz? and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.