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PENURUNAN KADAR GULA DARAH PADA MONYET EKOR PANJANG OBES DENGAN PEMBERIAN NIKOTIN DOSIS RENDAH (DECRESE IN BLOOD SUGAR LEVEL IN LONG TAILED OBESE MACAQUES GIVING WITH LOW DOSE NICOTINE) Choliq, Chusnul; Sajuthi, Dondin; Suparto, Irma Herawati; Astuti, Dewi Apri; Wulansari, Retno
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to evaluate the role of low dose nicotine on the profile of blood glucose and  â-cell of pancreatic islets.  Fourteen adult (aged 6 ? 8 years) male cynomolgus monkeys grouped based ontheir Body Mass Index (BMI) into preobese (BMI=23.65 ? 25.00) and obese (BMI e? 26.00) were used inthis study. Subsequently animals were grouped into four:  (i) preobese monkeys with nicotine (pOb+), (ii)obese monkeys with nicotine (Ob+), (iii) preobese monkeys without nicotine (pOb-), and (iv) obese monkeyswithout nicotine (Ob-). Animals in the nicotine groups were fed with high fat diet mixed with nicotine dose0.5?0.75mg/kg body weight/day for three months and the others were fed Monkey Chow® only as thecontrol group. Blood samples were collected every month for glucose analysis and necropsy was performedat the end of study. Pancreas tissues were processed histologically and stained using  immunohistochemicalmethod. The results showed that the blood glucose either preobese (28.37%) or  obese (33.72%) animals inthe nicotine groups significantly decrease (p<0.05) during the study period in comparison to the controlgroup.  Based on brown color intensity of granules cytoplasm of insulin producing cells or immunoreactiveâ-cells, it is shown that the cells of animals in the non nicotine group were more reactive than those in thenicotine groups. In conclusion, there was positive effect of low dose nicotine in maintaining the bloodglucose level in normal range by stimulation of islet cells proliferation to maintain the production ofinsulin in the pancreatic islet.
PREVALENSI DAN FAKTOR RISIKO INFEKSI PARASIT SALURAN PENCERNAAN PADA KERBAU LUMPUR DI KABUPATEN BREBES, JAWA TENGAH Nurhidayah, Nanis; Satrija, Fadjar; Retnani, Elok Budi; Astuti, Dewi Apri; Murtini, Sri
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2019.20.4.572

Abstract

Kerbau lumpur merupakan ternak multiguna yang memiliki kedekatan dengan kehidupan masyarakat di kawasan lumbung ternak khususnya di Kabupaten Brebes, Jawa Tengah. Infeksi parasit saluran pencernaan pada kerbau lumpur bedampak negatif terhadap kesehatan ternak sekaligus menyebabkan kerugian ekonomi. Data epidemiologi infeksi saluran pencernaan pada kerbau lumpur di Kabupaten Brebes belum tersedia. Penelitian ini bertujuan untuk mengukur prevalensi, derajat infeksi serta analisis faktor risiko infeksi parasit saluran pencernaan pada kerbau lumpur di Kabupaten Brebes. Sebanyak 240 sampel tinja diperoleh dari kerbau di empat kecamatan, yaitu; Salem, Bantarkawung, Tonjong dan Brebes. Hasil pemeriksaan mikroskopik dengan teknik Mc Master menunjukkan bahwa 27.5% kerbau terinfeksi oleh parasit saluran pencernaan. Infeksi disebabkan oleh Toxocara vitulorum (1.67%; telur tiap gram tinja [TTGT] 174.4), cacing Strongyle (Cooperia, Nematodirrus, Trichostrongylus) (4.17%; TTGT 140.18), Trichuris spp. (3.33%; TTGT 64.84), Moniezia expansa (9.17%; TTGT 122.47) dan Eimeria spp (21.25%; TTGT 162.37). Infeksi lebih tinggi tercatat pada kelompok jantan, umur muda (>12?30 bulan), kerbau di Kecamatan Salem dan yang dipelihara pada daerah bertipe iklim D4. Variabel umur merupakan satu-satunya faktor risiko infeksi yang ditunjukkan dengan ­P-value 0.03. Nilai odd ratio kelompok muda dan pedet adalah 3.424 dan 2.529, artinya, kelompok muda dan pedet memiliki risiko 3.424 dan 2.529 lebih tinggi dibandingkan kerbau dewasa (referensi). Dari penelitian ini dapat disimpulkan, baik prevalensi maupun derajat infeksi parasit saluran pencernaan di Kabupaten Brebes terjadi dalam taraf yang rendah dan dipengaruhi oleh faktor umur ternak.
PHYSIOLOGICAL STATUS, BLOOD PROFILE AND BODY COMPOSITION OF SHEEP FED WITH CA-SAPONIFIED LEMURU OIL COATED BY HERBS Astuti, Dewi Apri; Sudarman, Asep
Buletin Peternakan Vol 39, No 2 (2015): BULETIN PETERNAKAN VOL. 39 (2) JUNI 2015
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (715.034 KB) | DOI: 10.21059/buletinpeternak.v39i2.6716

Abstract

This study was aimed to evaluate the physiological status, blood profiles and body composition of sheep fed with Ca-saponified lemuru oil coated by herbs. Twenty fat-tailed sheep (av. BW 23±1.2 kg) were used in this experiment by using Completely Randomized Design with five treatments ration and four replications of each. Sheep fed with concentrate containing 3% Ca-saponified lemuru oil and king grass (1:1) ad libitum. Treatments were control diet without herbs (R1); Ca-saponified lemuru oil coated by curcumae domestica (R2); coated by Zingiber officinale Rosc (R3); coated by Eugenia polyantha (R4) and coated by Pluchea indica Less (R5). Data of physiological parameters were measured three times a day, in the morning, at noon and afternoon. Blood samples were collected at the end of the experiment through jugular vein, together with zero sample for Urea Space measurement. The variables observed were physiological data (heart rate, respiration rate, and rectal temperature), blood profiles (erythrocyte, hemoglobin, packed cell volume (PCV), leucocytes) and body composition (water, protein and fat) measured using Urea Space technique. Results of the study showed that sheep fed with Ca-saponified lemuru oil coated by herbs was not significantly different on heart rate, respiration rate and rectal temperature among treatments. Meanwhile, total leucocytes, neutrophil cell, and lymphocytes significantly increased (P<0.05) by the treatment. Body composition percentage (water, protein and fat) were same in all treatments, except the total body fat and energy retained. In conclusion, supplementation of 3% Ca-saponified lemuru oil coated by Curcumae domestica, Zingiber officinale Rosc, Eugenia polyantha and Pluchea indica Less in fat-tailed sheep had no effect on physiological parameters, but improved the leucocyte and neutrophil cells. Total body fat and energy retained lower compared to control treatment.(Key words: Body composition, Ca-saponified, Herbs, Lemuru oil, Urea space)
PEMANFAATAN BUNGKIL BIJI JARAK PAGAR (JATROPHA CURCAS) TERFERMENTASI SEBAGAI PAKAN AYAM KAMPUNG Sumiati, .; Astuti, Dewi Apri; Suharti, Sri
Jurnal Ilmu Pertanian Indonesia Vol. 15 No. 2 (2010): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Jatropha curcas"meal (JCM) is very potential as protein source for poultry. The JCM contained high crude protein, i.e. 56,4-63,8% (without hull) and 22,39-31,41% (hulled JCM). JCM serves as a highly nutritious and economic protein supplement in animal feed, if the toxins and antinutrients contained in the JCM are removed. The toxic compounds isolated from jatropha seed include curcin, phorbolesters, and the antinutrients include antitrypsins, tannin, saponin, phytic acid, and high fiber. The objective of this research was to evaluate the effect of feeding fermented JCM on the performances of kampong chickens. In this study, tempeh fungi (fungi using in fermenting soybean) used to ferment the JCM. Two hundred kampung chickens were used in this experiment and reared from day old up to 10 weeks of age. The data analyzed with a Completely Randomized Design with 5 treatment diets and 4 replications, with 10 birds in each replicate. The experimental diets were: TO (the control diet, without Jatropha curcas meal), T1 (the diet contained 5°/o untreated Jatropha curcas meal), T2 (the diet contained 5% fermented Jatropha curcas meal + cellulase 200 ml/ton of feed), T3 (the diet contained 5°/o fermented Jatropha curcas meal + 1000 FTU phytase), and T4 {the diet contained 5% fermented Jatropha curcas meal + cellulase 200 ml/ton + 1000 FTU phytase). The results showed that there were no significant difference on the parameters observed due to the treatments. Feeding fermented Jatropha curcas meal supplemented with cellulase+ phytase{T4) yielded the final body weight and feed conversion ratio similar to those the control {TO) diet. There was no mortality observed in all treatments. Using JCM 5% in the diet is safe for the kampong chickens.
LESIO ATEROSKLEROSIS PADA ARTERI ILIAK MONYET EKOR PANJANG SEBAGAI HEWAN MODEL PERIPHERAL ARTERIAL DISEASE (PAD) Laila, Sri Rahmatul; Astuti, Dewi Apri; Suparto, Irma Herawati; Handharyani, Ekowati; Sajuthi, Dondin
Acta VETERINARIA Indonesiana Vol. 7 No. 1 (2019): Januari 2019
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.568 KB) | DOI: 10.29244/avi.7.1.17-22

Abstract

Aterosklerosis pada arteri iliak berkaitan dengan kejadian peripheral arterial disease (PAD) pada manusia. Penelitian ini bertujuan untuk menganalisis lesio aterosklerosis pada arteri iliak sebagai salah satu faktor penyebab PAD. Penelitian ini menggunakan sembilan monyet ekor panjang jantan dewasa dari 3 kelompok respons yang diberi pakan aterogenik IPB-1 selama 2 tahun. Arteri common iliak diambil pada saat nekropsi, diproses menjadi slaid histologi dan diwarnai dengan Verhoeff van Giesson (VVG). Keparahan lesio dikategorikan sebagai grade I sampai VI dan dianalisis secara deskriptif. Area lesio ditentukan melalui panjang lamina elastik interna (PLEI), area intima (AI), serta ketebalan maksimum intima (KIM) dan dianalisis dengan ANOVA. Hasil penelitian menunjukkan 55,56% monyet membentuk lesio plak aterosklerosis (grade IV sampai VI) yang mana mereka berasal dari kelompok intermediet dan hiper-responsif. Kelompok hiper-responsif menunjukkan AI dan KMI paling tinggi (0,979 mm2; 0,352 mm) diikuti kelompok intermediet (0,372 mm2; 0,237 mm) dan kelompok hipo-responsif (0,049 mm2; 0,052 mm) (P&lt;0,05). Hasil ini menunjukkan semakin tinggi kolesterol plasma, semakin luas area lesio yang terbentuk. Keseluruhan hasil mendukung bahwa monyet yang diberi pakan aterogenik IPB-1 selama 2 tahun mampu membentuk lesio plak aterosklerosis pada arteri common iliak, dan berpotensi sebagai hewan model PAD, kecuali kelompok hipo-responsif.
BLOOD PROFILE OF QUAILS (COTURNIX COTURNIX JAPONICA) FED RATION CONTAINING SILKWORM PUPAE (BOMBYX MORI) POWDER EXTRACT Anggraeni, Novita; Farajallah, Achmad; Astuti, Dewi Apri
Media Peternakan Vol. 39 No. 1 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.491 KB) | DOI: 10.5398/medpet.2016.39.1.1

Abstract

The aim of this research was to assess the use of silkworm pupae (Bombyx mori) powder extract on the blood cells and hematological variables of quails (Coturnix coturnix japonica). The treatments consisted of R0= isoenergy and isoprotein ration without pupae powder extract, R1= isoenergy and isoprotein ration + 1% of pupae powder extract, R2= isoenergy and isoprotein ration + 10% of pupae powder extract, R3= isoenergy and isoprotein ration + 1% of residue of pupae powder extract, R4= isoenergy and isoprotein ration + 10% of residue of pupae powder extract, R5= isoenergy and isoprotein ration + 1% of pupae powder, and R6= isoenergy and isoprotein ration + 10% of pupae powder. The variables measured were blood cell and hematological profiles including the number of leukocytes, erythrocytes, and leukocytes differentiation. Immune response was observed at 12, 24, and 48 h by counting the white blood cells type. The results showed that feed consumption ranged 79.19-154.70 g/quail/wk and the body weight was 71.45-149 g/quail. The addition of pupae extract at a dose of 10% in the diet significantly (P&lt;0.05) increased the number of quail white blood cells. The addition of pupae extract, pupae powder residue, and pupae powder in the ration did not significantly affect the number of quail erythrocytes, hemoglobin, and PCV (packed cell volume). Mean number of white blood cells increased during 24 h after treatment of 10% pupae powder extract (R2) and decreased at the 48th h. It is concluded that the value of leukocytes, lymphocytes, monocytes, and heterophile increased in quails supplemented with 10% pupae powder extract (R2).
PENGUJIAN RANSUM KERBAU BERBAHAN BAKU SORGUM SEBAGAI SUMBER SERAT SECARA IN VITRO DAN IN SACCO Wahyono, Teguh; Astuti, Dewi Apri; Wiryawan, Komang G.; Sugoro, Irawan
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 10, No 2 (2014): Desember 2014
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jair.2014.10.2.2709

Abstract

Sorgum merupakan salah satu tanaman sumber serat untuk kebutuhan ransum kerbau yang potensial dikembangkan di Indonesia. Sorgum varietas Samurai 1 dan samurai 2 masing-masing merupakan hasil pemuliaan melalui mutasi radiasi yang berasal dari indukan sorgum varietas Pahat. Penelitian ini dilakukan untuk mengetahui potensi ransum yang mengandung sorgum samurai 2 sebagai sumber serat dibandingkan dengan ransum yang mengandung sorgum pahat dan bagas sorgum samurai 1. Potensi yang diamati adalah pengaruhnya terhadap laju pertumbuhan mikroba rumen kerbau (in vitro) dan degradasi bahan pakan (in sacco). Rancangan acak lengkap dengan 6 perlakuan dan 3 ulangan diterapkan dalam percobaan ini. Enam ransum yang diuji adalah: P1 (50% jerami sorgum pahat + 50% konsentrat), P2 (50% silase jerami sorgum pahat + 50% konsentrat), P3 (50% jerami sorgum samurai 2 + 50% konsentrat), P4 (50% silase jerami sorgum samurai 2 + 50% konsentrat), P5 (50% bagas sorgum samurai 1 + 50% konsentrat) dan P6 (50% silase bagas sorgum samurai 1 + 50% konsentrat). Peubah yang diamati adalah pH, konsentrasi amonia (NH3), Total Volatile Fatty Acid (TVFA), sintesis protein mikroba (teknik radioisotop 32P), degradasi Bahan Kering (BK), karakteristik degradasi BK, degradasi Neutral Detergent Fiber (NDF) dan karakteristik degradasi NDF. Hasil yang diperoleh menunjukkan bahwa konsentrasi NH3, laju pertumbuhan bakteri rumen dan degradasi NDF tertinggi dihasilkan ransum P4 dengan nilai masing-masing 24,87 mg/100 ml; 8,11 mg/2 jam/100 ml dan 31,96%. Konsentrasi TVFA dan pH antar perlakuan tidak berbeda nyata. Keenam perlakuan ransum mampu mendukung fermentasi dan kecernaan pakan didalam rumen namun perlakuan yang terbaik adalah ransum yang mengandung silase sorgum samurai 2. Kata kunci : sorgum, kerbau, in vitro, in situ, radioisotop 32P
The effect of physic conditions of Caliandra calothyrsus and its mixing with fresh Gliricidia sepium toward the consumption and nutrient digestibility of sheep ., Firdus; Astuti, Dewi Apri; Wina, Elizabeth
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 1 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.153 KB) | DOI: 10.14334/jitv.v9i1.423

Abstract

It is reported that feedstuff preparation affects intake and digestibility of the ration. The objective of this experiment was to know the effect of physic conditions of Caliandra calothyrsus and its mixing with fresh Gliricidia sepium toward the digestibility and nutrient consumption of sheep. Fourty nine (49) thin tail sheep with the average body weight of 15.42 kg (SD 2.68) was randomly assigned into one out of five treatment groups of greenery feed which composed of different fresh Penisetum sp. (FP), fresh Caliandra calothyrsus (FC), fresh Gliricidia sepium (FG), dried C. calothyrsus (DC) and steamed C calothyrsus (SC). Treatment A (70% FP + 30% FC), B (70% FP + 30% FG), C (70% FP + 15% SC + 15% FG), D (70% FP + 15% DC +15% FG) and E (70% FP + 15% DC + 15% FG). The treatments had been carried out for 90 days. Water was given ad libitum. Parameters recorded in this experiment were nutrient consumption (dry matter/BK, crude protein, energy) and nutrient digestibility (BK, crude protein, energy). Data recorded were processed by covariant analysis with body weight of sheep at the early experiment treatment as a covariant. Results of the experiment showed that there were significant difference (P&lt;0.05) between fresh, dried, and steamed C. calothyrsus and its mixing with fresh G. sepium toward the consumption and nutrient digestibility. The average of consumption and nutrient digestibility value were high in mixing treatment of 15% fresh C. calothyrsus with 15% fresh G. sepium.   Key words: Sheep, Caliandra calothyrsus, Gliricidia sepium, consumption, digestibility
Potential and utilization of Indigofera sp shoot leaf meal as soybean meal substitution in laying hen diets Palupi, Rizki; Abdullah, Luki; Astuti, Dewi Apri; ., Sumiati .
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 3 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v19i3.1084

Abstract

The objective of this study was to determine the potential of Indigofera sp shoot leaf meal to substitute soybean meal in poultry diet. One hundred and sixty laying hens of Isa Brown strain, at 30 weeks old were used in this study and kept in individual cages. A Randomized Completely Design was applied with four treatments and four replications. The treatments were four levels of soybean meal protein substitution by Indigofera sp shoot meal protein in the diets: The level were 0% (R0), 15% (R1), 30% (R2) and 45% (R3). Eggs were collected daily and were evaluated on: weight, shell, albumen, yolk, intensity of yolk and haugh unit. Results showed that the nutrients content of Indigofera sp shoot leaf meal were crude protein 28.98%, crude fat 3.30%, crude fiber 8.49%, calcium 0.52% and phosphorus content was 0.34%. Indigofera sp shoot leaf meal contained a complete amino acids. The vitamin A and ß-carotene were high, i.e 3828.79 IU/100g and 507.6 mg/kg, respectively. It is concluded that Indigofera sp shoot leaf meal is potential to be used as an alternative source of protein. Substitute 45% soybean meal protein with Indigofera sp shoot leaf meal in laying hen diets increase egg quality and increase intensity of yolk colour to 55.88%. Key Words: Egg Quality, Soybean Meal, Nutrients, Indigofera sp Shoot Leaf Meal
Effect of Sapindus rarak powder as feed additive on performance and lipid profile of broiler chicken infected by Eimeria tenella Pasaribu, Tiurma; Wina, Elizabeth; ., Sumiati; Setiyono, A; Astuti, Dewi Apri
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 4 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.383 KB) | DOI: 10.14334/jitv.v19i4.1099

Abstract

Sapindus rarak fruits have bioactive compound named saponin which are potential to inhibit growth of protozoa and reduce cholesterol. This study was conducted to evaluate effect of lerak (Sapindus rarak) powder as microparticle on performance and lipid profile of chicken broiler that was infected by Eimeria tenella. A total of150headsof DOCwere assign to recive on of5 treatments: T1 (K+, TAk, TSRa, TIE), T2 (K-, TAk, TSRa, IE), T3 (Sal, IE), T4 (Sra 2,5, IE), and T5 (Sra 1,25, IE); each treatment consisted of 6replications with 5birds per replications in battery cage for 34 days. All chickens except those in control positive treatment (non-infected, non-medicated) were inoculated orally with 6000 oocysts E. tenella on the 14th day of age. Variables measured were body weight gain, carcass, OPG (oocysts pergram of faeces), lipid profile, and mortality. Treatment with S. rarak, dose of 1.25g/kg was not significantly different from the salinomycin treatment on BWG and feed conversion. Percentage of carcassand organs and blood cholesterol concentration were not significantly differentin all treatments, but blood triglyceride at S. rarak addition with doseof 2.5, 1.25g/kg and salinomycin addition with dose of 0.5g/kg, and negative control were lower than positive control. While number of OPG in faeces (days 14-34) showed that S. rarak powder with dose of 2.5 and 1.25g/kg suppressed the development of oocystsof E. tenella. In conclusion, S. rarak microparticle (75µm) can be used as feed additive to replace salinomycinas coccidiostat. Key Words: Sapindusrarak Powder, Chicken, Performance, Blood Lipid Profile, Eimeria tenella