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ANTICANCER ACTIVITY OF CALANONE ON HELA CELL LINE Ekowati, Heny; Astuti, Indwiani; Mustofa, Mustofa
Indonesian Journal of Chemistry Vol 10, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.361 KB) | DOI: 10.22146/ijc.21467

Abstract

Calanone (coumarin derivate compound), isolated from Calophyllum sp. had been shown to have cytotoxic activity on leukemia L1210 cell line with IC50 = 59.40 mg/mL. Calanone presumed have anticancer activity on HeLa cervical carcinoma cell. This study was conducted to investigate the cytotoxic and apoptotic activity of calanone and its effect to p53 and p21 expression on HeLa cervical carcinoma cell. Cytotoxic assay of calanone was performed on HeLa cell line, using MTT assay. Apoptotic assay was performed on HeLa cell line incubated with calanone for 24 h, by immunofluororescence method, using fluorochromes ethidium bromide and acridine orange. Expression of p53 was examined on HeLa cell line, by PCR with p53 wild-type primer. Expression of p21 was examined on HeLa cell line, by immunohistochemistry method. 5-fluorourasil was used as positive control in cytotoxic, apoptotic assay, and p53 expression. The result showed that calanone has cytotoxic activity on HeLa cell line, with IC50 = 22.887 mg/mL, caused cytotoxicity through apoptotic mechanism, increase p53 tumor suppressor gene expression, while the p21 expression test showed a negative result.
Pengaruh Teknik Aplikasi Pestisida terhadap Derajat Keparahan Penyakit Paru Obstruktif Kronis (PPOK) pada Petani Mahawati, Eni; Husodo, Adi Heru; Astuti, Indwiani; Sarto, Sarto
Jurnal Kesehatan Lingkungan Indonesia Vol 16, No 2 (2017): Oktober 2017
Publisher : Master of Environmental Health Study Program, Faculry of Public Health, Faculty of Public

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.728 KB) | DOI: 10.14710/jkli.16.2.37-45

Abstract

Judul: Pengaruh Teknik Aplikasi Pestisida Terhadap Derajat Keparahan Penyakit Paru Obstruktif Kronis (PPOK) Pada PetaniLatar Belakang: Kabupaten Grobogan  sebagai sentra utama pertanian di Jawa Tengah yang banyak menggunakan pestisida mengalami peningkatan kasus PPOK sejak tahun 2010, dimana  etiologi PPOK berasal dari gene-enviroment interaction dan salah satu penyakit akibat kerja utama terkait paparan zat toksik di tempat kerja. Persentase terbesar (71%) penderita PPOK berdasarkan data rekam medis 2009-2012 di RS PKU Muhammadiyah Gubug Kabupaten Grobogan adalah petani. Penelitian ini bertujuan menganalisis  pengaruh teknik aplikasi pestisida terhadap derajat keparahan PPOK pada petani.Materi dan Metode: Penelitian ini merupakan studi kasus kontrol yang dilakukan di  Kabupaten Grobogan dengan 100 petani sebagai responden yang dipilih secara purposive. Pengumpulan data melalui wawancara, observasi, pemeriksaan klinis dan spirometri. Analisis data menggunakan uji korelasi rank spearman dan  regresi logistik ordinal.Hasil: Hasil penelitian menunjukkan bahwa 23% responden termasuk PPOK Sedang, 17% PPOK sangat berat, 7% PPOK ringan dan 3% PPOK berat. Sebagian besar responden menyatakan lama penyemprotan pestisida lebih dari 5 jam per hari (69%), seminggu lebih dari 1 kali (72%), waktu penyemprotan pada pagi/sore hari (95%). Sebagian besar responden (85%) sering melakukan penyemprotan pestisida tanpa memperhatikan arah angin dan melakukan penyemprotan pestisida melawan arah angin (42%),  saat angin kencang (36%) dan menggunakan baju/kain yang sudah terpapar pestisida untuk menyeka keringat (42%). Hasil uji statistik menunjukkan ada hubungan signifikan antata derajat keparahan PPOK dengan lama paparan pestisida per hari (p value=0,003) dan kesesuaian arah penyemprotan pestisida (0,000). Ada pengaruh signifikan lama paparan pestisida per hari (p value=0,004) dan kesesuaian arah penyemprotan pestisida (p value=0,000) terhadap derajat keparahan PPOK.Simpulan: Temuan baru dalam penelitian ini adalah teknik aplikasi pestisida yang terbukti berpengaruh terhadap derajat PPOK adalah lama paparan per hari dan kesesuaian arah penyemprotan pestisida. Implikasi hal tersebut terhadap kesehatan masyarakat yaitu diperlukannya sosialisasi prosedur kerja aman petani dalam menggunakan pestisida dengan menyempurnakan teknik aplikasi pestisida secara baik dan benar dalam pencegahan  PPOK. Disarankan lama paparan pestisida disarankan tidak melebihi 5 jam per hari dan dihindarkan penyemprotan pestisida melawan arah angin. AbstractTitle: Effect of Pesticide Application Technical to The Severity Degree of Chronic Obstructive Pulmonary Disease (COPD) on FarmersBackground: Chronic Obstructive Pulmonary Disease (COPD) is a disease whose etiology derives from gene-enviroment interaction and has been one of the major occupational diseases associated with exposure to toxic substances in the workplace. Result of analysis of medical record data 2009-2012 in PKU Muhammadiyah Gubug Sub-Province of Grobogan Regency, it is known that the biggest percentage of COPD patients (71%) are farmers. This research aimed to analyze the effect of technical pesticide application on the severity degree of COPD to farmers.Methods: This research is a case-control study which was conducted in Grobogan District with 100 farmers repondents, had been selected by purposive method sampling. Data was collected by interviews, observation, clinical examination and spirometry. Data was analysed by rank spearman correlation and ordinal logistic regression.Results: The results showed that were 23% respondents in moderate COPD, 17% very severe COPD, 7% mild COPD and 3% severe COPD. Most respondents had spraying time pesticide  more than 5 hours daily (69%), more than 1 time weekly (72%), mostly in morning/afternoon (95%). Most of respondents (85%) frequently sprayed pesticides regardless of wind direction, sprayed pesticides against wind direction (42%), sprayed pesticides when high speed winds (36%) and used “pesticide-exposed clothes” to wipe sweat ( 42%). The result of statistical test showed significant correlation of the severity degree of COPD with pesticide exposure daily (p value = 0,003) and suitability of pesticide spraying direction (0,000). There were significant effect of pesticide exposure daily (p value = 0,004) and suitability of pesticide spraying direction (0,000) to the severity degree of COPD.Conclusion: The new findings in this study are the technical application of pesticide that has been shown to affect the severity degree of COPD is the length of exposure daily and the suitability of the pesticides spraying direction. The implications of this to public health is the need for socialization of safe work procedures of farmers in using pesticides by perfecting technical aspects of pesticide applications properly and correctly in the prevention of COPD. It was recommended not to exceed 5 hours daily and avoid spraying pesticides against the wind direction. 
PENGARUH VITAMIN D TERHADAP EKSPRESI COX-2, KADAR CAMP, KADAR RENIN DARAH DAN TEKANAN DARAH SISTOLIK DALAM SISTEM RENIN ANGIOTENSIN ALDOSTERON Hermawan, Dessy -; Soejono, Sri Kadarsih; Sunarti, '; Astuti, Indwiani; Nang Agus, Zainal Arifin
Jurnal Kedokteran Brawijaya Vol 29, No. 2 (2016)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2016.029.02.5

Abstract

Banyak penelitian yang melaporkan hubungan antara kadar vitamin D dalam darah dengan hipertensi, hanya saja mekanismenya dalam sistem renin angiotensin belum dapat dijelaskan dengan pasti. Hal ini yang menarik untuk mempelajari mekanisme aksi vitamin D dalam dalam pengaturan tekanan darah di sistem renin angiotensin aldosteron. Rancangan penelitian ini adalah eksperimen laboratorik dengan pre-post test group design, yang di bagi menjadi dua tahap. Tahap pertama untuk mempelajari pengaruh vitamin D terhadap ekspresi COX-2 di sel macula densa ginjal dan tahap kedua untuk mempelajari mekanisme aksi vitamin D dalam pengaturan tekanan darah. Hasil yang di dapat bahwa sasaran aksi vitamin D pada sistem renin angiotensin aldosteron kemungkinan ada di dua bagian, yaitu ada yang di bagian up-stream cAMP dengan menekan ekspresi COX-2 di sel macula densa ginjal dan ada yang di bagian down-stream cAMP, yaitu dengan menekan langsung gen yang menyandi renin. Hal ini didukung dengan data bahwa tidak terjadinya akumulasi cAMP, peningkatan kadar renin dan TDS walaupun hewan coba telah diberi teofilin bersamaan dengan vitamin D.Kata Kunci: hipertensi, vitamin D, renin, cAMP, COX-2 dan tekanan darah sistolik
Peningkatan Jumlah Mikronukleus pada Mukosa Gingiva Kelinci Setelah Paparan Radiografi Panoramik Shantiningsih, Rurie Ratna; Suwaldi, Suwaldi; Astuti, Indwiani; Mudjosemedi, Munakhir
Majalah Kedokteran Gigi Indonesia Vol 20, No 2 (2013)
Publisher : Majalah Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mikronukleus merupakan salah satu tanda awal terjadinya kerusakan DNA yang ditemukan pada mukosa gingiva manusia setelah paparan radiografi dental panoramik.   Peningkatan jumlah mikronukleus terjadi paling tinggi pada hari ke-10 dan selanjutnya mengalami penurunan sampai dengan hari ke-14. Kelinci memiliki karakter dan periode turn-over mukosa gingiva yang hampir sama dengan manusia berkisar antara 10-12 hari. Tujuan penelitian ini untuk mengevaluasi apakah peningkatan jumlah mikronukleus pada mukosa gingiva kelinci setelah paparan radiografi panoramik.Sembilan ekor kelinci dibagi menjadi 3 kelompok untuk mewakili hari ke-3, 6 dan 9 setelah paparan radiografi panoramik. Sebelum dan sesudah diberikan paparan radiografi panoramik,  setiap  hewan  coba  dilakukan  apusan  pada  mukosa  gingiva  anterior  rahang  bawah  menggunakan cervical brush. Hasil apusan dilakukan pewarnaan dengan modifikasi Feulgen-Rossenbeck dan dihitung jumlah mikronukleus menggunakan mikroskop yang disambungkan dengan optilab. Analisis statistik dilakukan menggunakan paired t-test. Analisis statistik menunjukkan adanya perbedaan yang signifikan (p<0,05) antara jumlah mikronukleus sebelum dan 9 hari sesudah paparan radiografi panoramik. Akan tetapi tidak ditemukan perbedaan yang signifikan (p>0,05) antara sebelum paparan dibandingkan hari ke-3 dan ke-6 setelah paparan radiografi panoramik. Kesimpulang dari hasil penelitian ini sejalan dengan penelitian sebelumnya pada manusia bahwa peningkatan jumlah mikronukleus terjadi pada hari ke-9 setelah paparan radiografi panoramik. Hasil ini mengindikasikan bahwa pada kelinci juga menunjukkan peningkatan jumlah mikronukleus di mukosa gingiva akibat paparan radiografi panoramik.ABSTRACT: Micronucleus Increase After Panoramic Radiography Exposure In Rabbit’s Gingival Mucosa. Micronucleus is one of the early states of DNA damage found in human gingival mucosa after dental panoramic radiography exposure. The increasing amount of micronucleus will reach a peak in the tenth day after the exposure, and it will continuously decrease right after the fourteenth day. Rabbit has almost the same gingival mucosa and turn-over period with human for about 10-12 days. The purpose of this research is to evaluate the increasing amount of micronucleus in rabbit’s gingival mucosa after panoramic radiography exposure. A total of nine New Zealand rabbits were divided into 3 groups to represent day of 3rd, 6th  and 9th after the panoramic radiography exposure. The mandibular anterior gingival mucosa of each animals was swabbed using a cervical brush before and after panoramic radiography exposure. The samples were stained with Feulgen-Rossenbeck modification, and the amount of micronucleus was counted using a microscope that is connected to Optilab. Statistical analysis was performed using paired t-test. The statistical analysis showed that there was significant difference (p <0.05) between the number of micronucleus before exposure and 9th day after panoramic radiography exposure. Moreover, there was no significant difference (p> 0.05) between the amount of micronucleus before exposure compared with 3rd  and 6th  day after panoramic radiography exposure. Based on the experiment, it is concluded that the result is consistent with previous studies conducted in human that there was increasing amount of micronucleus at the 9th  day after panoramic radiography exposure. This result   indicates that rabbit   performs the increasing amount of micronucleus in gingival mucosa because of panoramic radiography exposure
The effect of active compound isolated from the leaves of kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line Mardihusodo, Hajid Rahmadianto; Hartati Wahyuningsih, Mae Sri; Astuti, Indwiani
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (908.662 KB) | DOI: 10.19106/JMedScie004503201301

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Colorectal cancer is the tenth most common form of malignant tumor of hospital inpatients inIndonesia. Advance approaches in anticancer development is discovery molecular-targeted drugs.Molecular targets for anticancer drug have been identified including genes associated with cellcycle control and angiogenesis. Previously, an active and selective compound against WiDr fromTithonia diversifolia (Hemsley) A. has been isolated. The aim of this study was to evaluate theeffect of the isolated active compound fromT. diversifolia on the WiDr cell cycle and angiogenesis.Isolation of the active compound was performed by preparative thin layer chromatography (TLC)method. WiDr cell cycle was analyzed by flowcytometry using propidium iodide (PI).Antiangiogenesis effect was evaluated by immunocytochemistry method using anti-human VEGFmonoclonal antibody. The results showed that the effect of the isolated active compound onthe WiDr cell cycle depended on the concentration and the incubation time periods. Atconcentration of 4 μg/mL, it inhibited the WiDr cell cycle SubG1 phase after 36 and 48 hoursincubation and G1 phase after 72 hours incubation. While at concentration of 8 μg/mL, it clearlyinhibited the WiDr cell cycle G1 phase after 36, 48 and 72 hours incubation. Furthermore, theisolated active compound at concentration of 4 μg/mL significantly inhibited the VEGF expressionuntil 47.38% compared to control. In conclusion, the isolated active compound fromT. diversifoliainhibited cell cycle and angiogenesis of WiDr cell.
CYP3A4*1G gene Polymorphism on Javanese People Sutrisna, Em; Dwiprahasto, Iwan; Astuti, Indwiani; Kristin, Erna
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.035 KB)

Abstract

AbtractMost of drugs are metabolized by cytochrome P 450 (CYP) enzyme. Cytochrome P450 3A4 is the cytochrome that is involved in metabolizing more than 60% of all medicine used in human. The variation of this CYP3A4 gene will affect the catalytic activity of this enzyme. Recently, CYP3A4*1G in intron 10 was found in Chinese and Japanese population. There is a substitution of G to A at position 82266 in intron 10. The purpose of this research was to investigate the frequency of allele and genotype CYP3A4*1G. Samples were taken from bloods of the subjects of the research. The examination of CYP3A4*1G was conducted by RTLP-PCR method.As the results of this research, the frequency of CYP3A4*1G in Javanese people is CYP3A4*1/*1 0.25, CYP3A4*1/*1G 0.55 and CYP3A4*1G/*1G 0.20. Frequency of allele G: 0.53, allele A: 0.47. The Fisher’s exact- test shows that the allele and genotype frequencyis p. 1.000. The allele and genotype frequency of Javanese people isstill in Hardy-Weinberg equilibrium.Keywords : CYP3A4*1G gene, polymorphism, Javanese people
Determinants Levels of Urinary 8-Hydroxydeoxyguanosine among Chromium Electroplating Workers Setyaningsih, Yuliani; Astuti, Indwiani; Husodo, Adi Heru
International Journal of Public Health Science (IJPHS) Vol 5, No 4: December 2016
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.261 KB) | DOI: 10.11591/ijphs.v5i4.4845

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Electroplating workers were exposed by particulate and fog of chromium from electrolysis process during work. Chromium is highly carcinogenic when inhaled.  Chromium can be reduced in the cells in the body. This process may generate Reactive Oxygen Species (ROS) causing lipid peroxidation and oxidative DNA damage. This research was aimed to analyse the influence of chromium to the levels of urinary 8-OHdG as an indicator to support early diagnosis of occupational disease. 66 electroplating workers in Tegal District were taken purposively as samples for this research. There were association between chromium level in urine, malondialdehyde (MDA) level, Body Mass Index, smoking habit, alcohol and supplement drink consumption and urinary 8-OHdG (p<0.05). There was not association between BMI and urinary 8-OHdG (p>0.05). Multivariate analysis using logistic regression showed the alcohol consumption had the most powerful influence to the level of urinary 8-OHdG. The levels of urinary 8-OHdG could be used as an indicator of oxidative DNA damage and early diagnosis of occupational disease among electroplating workers who were exposed by chromium.
Dust Exposures, IgE Levels, History of Allergy, and Symptoms of Allergy in Poultry Workers Wijayanti, Yuni; Sutomo, Adi Heru; Astuti, Indwiani; Asmara, Widya
KEMAS: Jurnal Kesehatan Masyarakat Vol 14, No 1 (2018)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v14i1.10232

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Poultry dust exposure may increase workers’ health risks, particularly in the form of respiratory allergic reactions. This study aimed to identify mold content of the dust and to analyze the association between dust exposure, IgE level, history of allergy, and symptoms of allergy. This study used cross sectional design with total samples of 33 workers. The data were analyzed using chi-square test and multivariate logistic regression. This study found 93.33% growth of Aspergillus sp., 69.7% work duration > 3 years, 84.8% high IgE levels, 18.2% history of allergy, and 69.7% symptoms of allergy. Dust exposure and history of allergy did not show significant correlation with symptoms of allergy while IgE levels had significant correlation with p-value of 0.036. Workers with high IgE-level were 15.986 times more likely to have symptoms of allergy (p-value 0.028). Aspergillus sp. as dust allergen potentially increased IgE levels and might become the base for facilitation of early and independent preventive and promotive efforts of workers’ health.
Polymorphism of Transcription Factor 7-Like 2 Gene and HOMA-β Level of Individuals With and Without Type 2 Diabetes Mellitus Family History Sahrani, Waode Astria; Astuti, Indwiani; Sadewa, Ahmad Hamim
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.526 KB) | DOI: 10.22146/ijbiotech.9312

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Family history has considered as a risk factor of type 2 diabetes. Transcription factor-7 like 2 (TCF7L2) hasrole to regulates insulin secretion and blood glucose homeostasis. The aim of current study was to determine thers7903146 polymorphism of TCF7L2 gene and homeostatic model assessment-β (HOMA-β) level on individual withand without type 2 Diabetes Mellitus (DM) family history. This work is a case-control study. Thirty six subjectswith type 2 DM family history and 36 subjects without type 2 DM family history were recruited. HOMA-βmeasure to analyze the insulin secretion. Polymorphisms of TCF7L2 gene was analyzed by using PCR-RFLPmethod. Statistical analysis was performed by using T-test, Mann-Whitney and Chi-square with signifi cancelevel 0.05. The frequency of the T allele of the cases were 4.2% and the controls were 2.8% (p=0.500). The oddratio was 0.649 (CI;95%:0.106-4.055). The HOMA-β levels of the cases were signifi cant low (132.56±62.48)compared with the controls (266.09±1.68) with p=0.000. The subjects with type 2 DM family history have asimilar frequency of having T alleles and CT/TT genotypes. The subjects with type 2 DM family history hassignifi cantly lower HOMA-β levels than subject without DM family history.
Systemic IL-1β and TNF-α Productions of E. coli Lipopolysaccharide-Induced Periodontitis Model on Rats Jonarta, Alma Linggar; Asmara, Widya; Astuti, Indwiani; Tandelilin, Regina TC
The Indonesian Journal of Dental Research Vol 1, No 1 (2010)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (690.622 KB) | DOI: 10.22146/theindjdentres.9988

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Periodontal disease, a common inflammatory oral disease involved periodontal tissues, has been linked with the evidence of some systemic disorders. Recently, periodontal disease has been suspected as a trigger of systemic disorders. Penetration of bacterial products, such as lipopolysaccharide (LPS) may reach into deeper periodontal tissues. Therefore there may affect systemic blood and cytokines production. Interleukin-1β (IL-1β) and Tumour Nuclear Factor-α (TNF-α) are known as pro-inflammatory cytokines. The production of systemic IL-1β and TNF-α of E. coli lipopolysaccharide-induced periodontitis model on rats was investigated in this research. Fifteen male Wistar rats, aged 6-8 weeks used for this study were divided into 3 groups. For group 1 and 2, silk ligature 3/0 were inserted in interdental area between upper right molar 1 and 2. First and second group received solution containing 10μg/ml and 1mg/ml E. coli lipopolysaccharide, respectively, mixedwith 2% carboxymethylcellulose (CMC) diluted in 100μl of phosphate buffer saline (PBS). The solution was topically applied on gingival tissues around the gingival sulcus, a single topical application of solution onceper 2 days for 14 days. Untreated subjects were used as negative control. On day 15, the blood was collected from vena orbitalis, and rats were sacrificed. The blood serum of each group was divided into 2 groups andcultured for 4 hours with or without 20μl of 100ng/ml of E. coli LPS. ELISA techniques were used to measure the cytokine productions of the supernatant. The data was analysed using Repeated Measure ANOVA. This study showed that there was a significant increase of IL-1β production on low dose of LPS compared to control and high dose of LPS groups (p<0.05). Whereas TNF-α not significantly showed increasing trend. The increasing trend of pro-inflammatory cytokine productions, such as IL-1β and TNF-α, on LPS-induced periodontitis model in this experiment supports the previous studies about the contribution of periodontal disease in the pathogenesis of systemic diseases.
Co-Authors . Jumina . Mustofa ., Ysrafil Adi Heru Husodo Adi Heru Sutomo Agus, Zaenal Arifin Nang Ahmad Hamim Sadewa Ahmad Husain Asdie Akhmad Edy Purwoko, Akhmad Edy Alma Linggar Jonarta, Alma Linggar Ana Lucia Ekowati, Ana Lucia Arief Budiyanto, Arief Arifin Nang Agus, Zainal Aryandono, Teguh Aryandono, Teguh Awal Prasetyo Bambang Hariwiyanto Bambang hermani Brilliana Nur Rohima, Brilliana Nur Daniel Ardian Soeselo, Daniel Ardian Devi Usdiana Rosyidah, Devi Usdiana Dibyo Pramono Didik Setyo Heriyanto, Didik Setyo Djaswadi Dasuki Em Sutrisna, Em Endang Mahati Eni Mahawati Erna Kristin GF, Torizal Hadi, Cahyono Hajid Rahmadianto Mardihusodo, Hajid Rahmadianto Hari Kusnanto Harijadi, . Hatmansjah, Hatmansjah HENY EKOWATI Herawati, Cita Hermawan, Dessy Hermawan, Dessy Hermawan, Dessy - Hery Suwito, Hery Isadora Gracia, Isadora Iwan Dwiprahasto Jumina Jumina Kadarsih, Sri Kadarsih, Sri Kartika, Yolanda Dyah Kartika, Yolanda Dyah Laksono Trisnantoro Lorettha Wijaya Lukman Anwar, Sumadi Lutfan Lazuardi Mae Sri Hartati Wahyuningsih, Mae Sri Mohammad Juffrie, Mohammad Muhammad Ghufron Munakhir Mudjosemedi Mustofa Mustofa N, Sa’adah Novilla, Arina Novilla, Arina Nyoman Kertia Oktriani, Risky Pramono, Zacharias Aloysius Dwi Pratiwi, Woro Rukmi Purba, Abdul Khairul Rizki Purba, Abdul Khairul Rizki R, Oktriani Rasuane Noor, Rasuane Regina TC Tandelilin, Regina TC Rianto, Bambang Uji Djoko Rina Susilowati Roy David Sarumpaet, Roy David Rurie Ratna Shantiningsih, Rurie Ratna Sagung Rai Indrasari Sarto Sarto Sitarina Widyarini Soenarto Sastrowijoto, Soenarto Soesaty, Marsetyawan Heparis Nur Ekandaru Sofia Mubarika Sofia Mubarika Haryana Sri Kadarsih Soejono Sugiyanto Sugiyanto Sunarti . Sunarti Sunarti Sunarti, ' Supriatno S., Supriatno Suprihati ., Suprihati Sutjipto Sutjipto suwaldi suwaldi T, Wardana Totok Utoro Waode Astria Sahrani, Waode Astria wardana, Tirta Widya Asmara Widyasari, Anis Yudanti Riastiti Yuliani Setyaningsih Yuni Wijayanti Zainal Arifin Nang Agus