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PERTUMBUHAN SAWI YANG BERASOSIASI DENGAN BAKTERI SYNECHOCOCCUS SP. PADA BERBAGAI KONDISI MEDIA SALINITAS Asmuni, -; Avivi, Sholeh; Winarso, Sugeng
Agrovigor Vol 10, No 1 (2017): Maret
Publisher : University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v10i1.2844

Abstract

Sawi hijau merupakan salah satu jenis sayur yang di konsumsi oleh masyarakat Indonesia yang memiliki banyak manfaat diantaranya untuk mencegah kanker, hipertensi, penyakit jantung, membantu kesehatan sistem pencernaan, mencegah dan mengobati penyakit pellagra, serta menghindarkan ibu hamil dari anemia. Tanaman sawi pada umumnya dapat hidup di dataran rendah maupun dataran tinggi, akan tetapi lebih baik penanamannya dilakukan di dataran tinggi. Penelitian tentang resistensi sawi di media salin belum banyak dilakukan. Tujuan dari penelitian ini adalah untuk mengetahui respon tanaman sawi hijau yang telah berasosiasi dengan bakteri Synechococcus spterhadap berbagai kondisi salinitas. Penelitian ini dilakukan mulai bulan November 2016 sampai Januari 2017 di Desa Bendoarum Kecamatan Wonosari Kabupaten Bondowoso. Adapun bahan tanam yang digunakan adalah sawi, biakan bakteri fotosintetik (Synechococus sp), air, tanah, pasir, garam NaCl, pupuk (urea, SP-36 dan KCl). Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan 4 kali ulangan. Perlakuan salinitas diberikan berdasarkan penambahan konsentrasi garam (NaCl) yaitu kontrol (0 ppm), 2500 ppm, 5000 ppm, 7500 ppm, dan 10.000 ppm. Respon ta tanaman terhadap perlakuan didasarkan pada variabel jumlah daun, jumlah klorofil, panjang akar, volume akar, berat basah, dan berat kering. Data tersebut dianalisis menggunakan uji Anova dan Jarak Berganda Duncan (DMRT) dengan signifikansi pada taraf 5%. Respon tanaman sawi terhadap penambahan garam menunjukkan hasil yang berbeda nyata pada semua parameter pertumbuhan yang meliputi jumlah daun, jumlah klorofil, panjang akar, volume akar, berat basah, dan berat kering. Respon tanaman yang tercekam garam baik yang diberikan bakteri Synechococcus sp atau yang tidak diberikan menunjukkan hasil menurun dan berbeda nyata pada parameter panjang akar, berat basah, dan berat kering. Pemberian bakteri Synechococcus sp pada tanaman yang ditanam dilahan salin tidak berpengaruh nyata pada parameter pertumbuhan tanaman, tapi cenderung meningkatkan berat basah tanaman, berat kering tanaman, dan panjang akar tanaman.Kata kunci : Salinitas, Sawi Hijau, Synechococcus sp, pertumbuhan tanaman
REGENERASI EMBRIOGENESIS SOMATIK PADA BEBERAPA KLON KAKAO INDONESIA DARI EKSPLAN BUNGA Avivi, Sholeh; Prawoto, Adi; Oetami, Reny Fauziah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.211 KB) | DOI: 10.24831/jai.v38i2.1798

Abstract

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> This research was aimed to observe the response of different clones and speci?  c organs due to the somatic embryogenesis regeneration. It was arranged in factorial randomized completely design with three replication. The ?  rst factor was cocoa clones i.e. ICCRI 01, ICCRI 02, ICCRI 03, ICCRI 04, KW 514, RCC72, and Sca 6. The second factor was ?  ower parts i.e. petal, staminode and anther. Every explant was regenerated on initiation, induction, multiplication and rooting media. Almost all treatments showed high response of embryogenic calli which range 89.5 to 100% at initial stage, but different results were found at the following process of somatic embryogenesis. The experiment showed that each clones and each different part of ?  ower had different response to somatic embryogenesis. The highest response of the explant number resulted from Sca 6 clone, which produce 35.8% embryo with average number of embryo per explant (1.34) followed by RCC 72 (28.4%, averaged 0.7) ICCRI 03 (24.7%, averaged 1.3) ICCRI 04 (18.6%, averaged 0.6). While ICCRI 02 showed the lowest responsive clone. Especially for ICCRI 01, 55.8% explant was rooted and  only 1.3% explant producing embryo. The highest response of somatic embryo was resulted form petal. Keywords: somatic embryogenesis, ? ower explant, Cocoa
MORPHOLOGICAL DIVERSITY AND MOLECULAR RAPD MARKERS OF SUGARCANE MUTANE (SACCHARUM OFFICINARUM L.) IN INUNDATION TOLERANCE Avivi, Sholeh; Suliswanto, Eko Nur; Restanto, Didik Pudji; Miswar, Miswar; Syamsunihar, Anang; Soeparjono, Sigit; Hartatik, Sri
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.1304

Abstract

The study aimed to identify variations in morphological and molecular character of sugarcane mutants from the mutation of Ethyl Methane Sulphonate (EMS). It used 21 sugarcane mutants and two non-mutant PS865 plants as controls. The treatments with inundation were carried out on 1-17 mutants and non-mutants, while the treatments without inundation were carried out on 18-21 mutant plants and non-mutants. The tolerance characteristics base on the agronomic characters. The RAPD molecular character was observed to detect changes in genotypes and kinship relationships of the plants tested. The results showed that the characteristics of tolerance to the best inundation were found in mutants 1, mutants 3 and mutants 6 which were characterized by the root volume (cm3), fresh root weight (g), sucrose content and brix value (%). Mutant plants treated with inundation showed higher levels of sucrose and brix values. Whereas the control plants in fats, showed lower levels of sucrose and brix. Changes in sugarcane mutant genotypes from non-mutants based on RAPD markers ranged from 14.7 - 56.7 % which resulted in an average polymorphic band of 35.1 % from 37 DNA bands and produced four main groups based on dendrogram analysis.
REGENERATION OF COCOA ZYGOTIC EMBRYO USING KINETIN IN B5 MEDIA Avivi, Sholeh
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.974 KB)

Abstract

The objective of this research was intended to identify the influence of kinetin application on zygotic embryo regeneration of several cocoa plants and examine the most appropriate kinetin concentration for regeneration of several cocoa clones. This research was conducted at Laboratory of Plant Tissue Culture, Agronomy Department, Faculty of Agriculture,Jember University. The research was designed by Completely Randomized Factorial Design within four replications. The first factor was 4 cocoa clones which consisted of DR 1, DR 2, ICS13 and ICS60.The second factor was 5 methods of zygotic embryo regeneration. Result showed that the best response of cocoa clone to kinetin concentration of all examined parameters was shown by DR 1 clone. Morever, on initiation stage, the most appropriate kinetin concentration for regeneration of several cocoa clones was 2 ppm.
TOLERANSI BERBAGAI VARIETAS TEBU TERHADAP PENGGENANGAN PADA FASE BIBIT BERDASARKAN KARAKTER MORFOLOGI DAN ANATOMI Avivi, Sholeh; Syamsunihar, Anang; Soeparjono, Sigit; Chozin, dan Muhammad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (596.239 KB) | DOI: 10.24831/jai.v46i1.14081

Abstract

Identification of Indonesia sugarcane varieties tolerant to waterlogging has not been done extensively. Information on varieties tolerant to waterlogging is required for seedling establishment in waterlogged areas. The purpose of this research was to identify the sugarcane varieties responses to several duration levels of waterlogging at seedling stage. The planting materials used were collection of Sugar Factory Semboro including VMC 76-16, BL, PS 862, PS 864, and PS 881. This research was arranged in randomized complete block design (RCBD) with two factors and three replications. The first factor was 5 varieties; the second factor was waterlogging treatment with four levels, i.e. without waterlogging, 2, 4, and 6 weeks of waterlogging in the bucket. The results showed different level of tolerance to water logging among the varities. PS 881 and VMC 76-16 varieties were the most tolerant to waterlogging supported by its ability to maintain plant height, root volume, root and shoot dry weight after been waterlogged for 6 weeks. These varieties were also able to establish aerenchyme tissue and increased the stomatal density. PS 862 was the least tolerant variety to waterlogging. Keywords: aerenchyme, morphology, stomatal density, waterlogging, water stress
EFISIENSI SERAPAN UNSUR 15N-UREA DAN PROPORSI FIKSASI N OLEH TANAMAN KEDELAI TIDAK BERKOTILEDON PADA BUDIDAYA JENUH AIR Avivi, Sholeh; Mugnisjah, Wahju Q.; Idris, Komarudin; Sisworo, Elsye L.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 25 No. 1 (1997): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1282.858 KB) | DOI: 10.24831/jai.v25i1.1654

Abstract

This pot experiment was to evaluate the influences of cotyledons detachment at seedling stage on the efficiency of labelled N-urea uptake and proportion of N-fixation by soybean cv. Wilis grown under saturated soil culture. Based on the result of preliminary experiment, cotyledons detachment was held at 7 days after sowing (das). The cotyledons detachment significantly reduced N-urea uptake efficiency and N-fixed proportion. The N-urea uptake efficiency at 21 days, R4, R5, R6, and R8 stages by cotyledons-detached plant were 30.5 %, 24.6 %, 23.7 %, 24.0 %, and 22.45 of the total N assimilated. Those of the cotyledons undetached-plant (control plant), the value were 31.6 %, 24.05, 24.7 %, 2.7 %, and 23.6 % respectively. At R8 stage, the cotyledons-detached plant has the amount of N-fixed proportion of 54.2 and N-soil of 23.4 %, whereas those of the control one had the amount of 49.7 and 26.7 % respectively. N-urea uptake, N-fixed, and N-soil uptake by the cotyledons- detached plant were lower than that of the control one (i.e. 69, 80, and 645 of the control respectively). The cotyledons detachment also result in inferior vegetative and productive growth of the plant in terms of decreased root dry weight (21.1 %), leave dry weight (18.8 %), plant dry weight (26.7 %), pot dry weight (23.8 %), grain number/pot (32.8 %), and grain dry weight (26.9 %). It seems that the lower growth and yield of the cotyledons-detached soybean paint were caused by the lower total N-uptake due to cotyledons detachment.
PENGARUH UKURAN EMBRIOZIGOT TERHADAP REGENERASI BEBERAPA KLON KAKAO Avivi, Sholeh; Restanto, Didik Pudji; Widyastuti, Tri
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.245 KB) | DOI: 10.31258/jnat.13.3.237-243

Abstract

This sesearch identified the responses of size of zygotic embryo of cocoa and to identify the responses of cocoaclone types toward ability to produce somatic embryo. The research was designed by Completely RandomizedFactorial Design within five replications. The first factors were the sizes of used explants, namely small (2-5 mm),average (6-9 mm) and big (10-15 mm). The second factors were cocoa clones, namely RCC 72, Sca 6, KW 162, KW163 and KW 165. The result showed that the best explant was the small size (2-5 mm) of cocoa zygotic embryo. Ithad significantly on number of rooting embryos and number of embryos with buds on rooting media. Clone RCC 72was the most responsive clone and could generate to be plantlet. Clone KW 165 performed the lowest response.
MULTIPLIKASI TUNAS DAN AKLIMATISASI TIGA VARIETAS PISANG: RAJA NANGKA, KEPOK, DAN MAS Avivi, Sholeh; Hardjo Soedarmo, Soetilah; Andi Prasetyo, Priyanto
Jurnal Hortikultura Indonesia Vol. 4 No. 2 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.657 KB) | DOI: 10.29244/jhi.4.2.83-89

Abstract

ABSTRACTRaja  Nangka,  Kepok,  and  Mas  are  local  popular  cultivar  of  banana  in Indonesia.  Mass propagation is needed for extended area of planting. The aims of this research was to determine the optimum  concentration  of  plant  growth regulator  for  planlet  regeneration.  The  research  was conducted at Department of Agronomy, Faculty of Agriculture, University of Jember.  We used three stages regeneration process: multiplication, rooting, and acclimatization. The multiplication stage was arranged in factorial experiment in a  completely  randomized  design with five replications. The first factor was kinetin concentration (3.5  ppm, 4.0  ppm, 4.5 ppm, 5.0 ppm, 5.5  ppm,  and 6.0 ppm) and the second factor was kind of banana (Raja Nangka, Kepok, and Mas). The rooting stage was also  arranged in factorial   completely  randomized design with five replication s  with the first factor was NAAconcentration  (0, 0.5, 1.0, and 1.5 ppm)  and the second factor was kind of banana. The result showedthat the best kinetin concentration on the multipliction stage was 5.0 ppm (the number of shoot 2.6). Raja  Nangka variety gave the highest number of shoot (2.7 shoot per  explant). The best concentration  atrooting stage was 0.5 ppm NAA which gave the number of root 7.5 and 3.2 cm  in  length. Most of planlet (90%) could be acclimatized on acclimatization media.Key words: acclimatization, kinetin, multiplicatin, NAAABSTRAKPisang Raja Nangka,  Kepok, dan  Mas  merupakan jenis pisang lokal yang terdapat di Indonesia dan digemari mas yarakat. Penelitian ini bertujuan untuk mengetahui konsentrasi zat pengatur tumbuh yang sesuai untuk regenerasi hingga menghasilkan planlet. Penelitian dilaksanakan di Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Jember. Penelitian terdiri ata s 3 tahap yaitu tahap m ultiplikasi tunas, tahap per akaran dan tahap aklimatisasi. Tahap multiplikasi  dilakukan  dengan  Rancangan  Acak  Lengkap faktorial  dengan  2  faktor.   Faktor  pertama  adalah konsentrasi  kinetin (3. 5 ppm ,   4. 0   ppm ,  4.5   ppm , 5.0   ppm , 5. 5   ppm ,  dan 6. 0 ppm) dan faktor kedua adalah jenis pisang ( Raja Nangka, Kepok, dan Mas) . Pada  tahap perakaran tunas hasil multiplikasi digunakan  Rancangan Acak Lengkap faktorial dengan dua  faktor. Faktor pertama adalah konsentrasi  NAA (0  ppm , 0.5 ppm , 1.0  ppm , dan  1. 5 ppm) ,   s edangkan  faktor  kedua  adalah  jenis  pisang dengan  taraf  yang  sama.  Ulangan dilakukan 5 kali. Uji lanjut dilakukan dengan uji Duncan 5%. Hasil penelitian menunjukkan bahwa konsentrasi kinetin terbaik diperoleh pada perlakuan  5. 0   ppm   (rata - rata  ju mlah  tunas 2.6). Jenis  pisang  Raja  Nangka  memberikan  rata- rata  ju mlah  tunas tertinggi sebanyak 2. 7 tunas  per - eksplan. Pada tahap perakaran, panjang akar opt imal diperoleh pada perlakuan 0. 5 ppm  NAA,  dengan  rata - rata  panjang akar  3. 2  cm  dengan  jumlah  akar  rata - rata  sebanyak   7. 5   buah . Tingkat keberhasilan aklimatisasi mencapai hingga 90%.Kata kunci: aklimatisasi, kinetin, multiplikasi, NAA
Efektivitas Gen CP PSTV dalam Memproteksi Nicotiana benthamiana Transgenik TO terhadap Serangan Peanut Stripe Virus Avivi, Sholeh; Sudarsono, Sudarsono; Ilyas, Satriyas; Aswidinnoor, Hajrial
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12103

Abstract

The aims of this research were: (1) to obtain the transgenic Nicotiana benthamiana T0 which have various cp Peanut stripe virus (PStV) genes construct (pBINRCP 1, pBINRCP2, pBINRCP3, and pBINRCP4); (2) to investigate the resistance of transgenic N. benthamianaT0 toward PStV infection; (3) to investigate the effectiveness of those construct to protect N. benthamiana T0 toward PStV infection. To achieve those objectives N. benthamiana T0 (70 plants) were regenerated, PCR tested and infected with PStV using biological analysis methods. The result showed that all of PStV construct gave the resistancy toward PStV infection. The phenotypic respon of those plants were the PBINRCPl plants 35.7% resistance, 0% recovery, and 64.3% susceptible, the PBINRCP2 plants 41.7%, resistance, 0% recovery, and 58,3% susceptible, the PBINRCP3 plants 71.4% resistance, 0% recovery, and 28.6% susceptible, and the PBINRCP4 plants 25.0% resistance, 25.0% recovery, and 50.0% susceptible.
CORRELATION BETWEEN SECONDARY METABOLITES OF LEAF AND THE RESISTANCE TO LEAF RUST (HEMILEIA VASTATRIX) ON SEVERAL ARABICA COFFEE CLONES Subroto, Gatot; Kusbianto, Dwi Erwin; Avivi, Sholeh; Slameto, Slameto; Setiyono, Setiyono
Jurnal Ilmu Pertanian Vol 4, No 2 (2019): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.61 KB) | DOI: 10.22146/ipas.42124

Abstract

Indicator of coffee resistance to leaf rust attack (Hemileia vastatrix) is needed to select superior coffee plants resistant to biotic stress. This study aims to find the relationship between the content of secondary metabolites and the intensity of leaf rust attack, so that it becomes a reference in the selection of future coffee plants. The experimental design used was a completely randomized design (CRD) with several coffee clones as a treatment. This test tested 5 Arabica coffee clones consisting of Komasti, Maragogik, Usda, Andong sari, and HDT clones. Each consists of 3 replications, and each replication consists of 2 sample plants. Observations were made by observing the intensity of the attack, and plant metabolites such as phenolic content, flavonoids and antioxidant activity were observed when the leaves had been attacked by Hemileia vastatrix. Correlation of leaf rust attack levels with phenolic content, flavonoids and antioxidant activity showed a relationship between each observation variable. Flavonoid content in certain conditions can be used as an indicator to get Arabica coffee plants that are resistant to the attack of leaf rust.