Moch. Saiful Bachri
Fakultas Farmasi, Universitas Ahmad Dahlan Yogyakarta

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EFEK HEPATOPROTEKTIF SERBUK AKAR PASAK BUMI (Eurycoma longifolia Jack.) DILIHAT DARI AKTIVITAS SGPTSGOT TIKUS JANTAN YANG DIINDUKSI CCl4 Adikusuma, Wirawan; Bachri, Moch. Saiful
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.189 KB) | DOI: 10.12928/pharmaciana.v4i2.1574

Abstract

This study was designed to evaluate the hepatoprotective effect of the powder Eurycomalongifolia Jack. From the activity level of SGPT-SGOT on CCl4-induced in male rats. Twentyfive male rats (150-250 g) divide in to 5 groups. Group I treated with aquadest was kept asnormal, group II treated with a single dose of CCl4 (1 ml/ kg BW i.p), group III and IV weretreated with Eurycoma longifolia Jack. (100 mg/kg BW and 200 mg/kg BW p.o) respectivelyand CCl4 (1 ml/kg BW i.p), group V treated with a single dose of curcumin (100 mg/kg BWp.o) and CCl4 (1 ml/kg BW i.p). Blood was collected from vena porta for determination ofSGPT-SGOT. The study showed the activity level of SGPT from the rats was treated byEurycoma longifolia Jack. 100 mg/kg BW and 200 mg/kg BW, Curcumin, and control groupsare 150.0±5.099 U/L; 113.6±5.508 U/L; 60.5±2.887 U/L; and 129.0±6.055 U/L respectively. Mean while the activity level of SGOT from the rats was treated by Eurycoma longifolia Jack.100 mg/ kg BW and 200 mg/ kg BW, Curcumin, and control groups are 369.4±11.165;263.0±1.803; 194.5±7.448; and 451.5±16.759 U/L respectively. The Eurycoma longifoliaJack. powder and Curcumin significantly (p < 0.05) declines two enzymes (SGPT and SGOT)than control group. The results concluded that Eurycoma longifolia Jack. powder hashepatoprotective effect.
EFEK HEPATOPROTEKTIF SERBUK AKAR PASAK BUMI (Eurycoma longifolia Jack.) DILIHAT DARI AKTIVITAS SGPTSGOT TIKUS JANTAN YANG DIINDUKSI CCl4 Adikusuma, Wirawan; Bachri, Moch. Saiful
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.189 KB) | DOI: 10.12928/pharmaciana.v4i2.1574

Abstract

This study was designed to evaluate the hepatoprotective effect of the powder Eurycomalongifolia Jack. From the activity level of SGPT-SGOT on CCl4-induced in male rats. Twentyfive male rats (150-250 g) divide in to 5 groups. Group I treated with aquadest was kept asnormal, group II treated with a single dose of CCl4 (1 ml/ kg BW i.p), group III and IV weretreated with Eurycoma longifolia Jack. (100 mg/kg BW and 200 mg/kg BW p.o) respectivelyand CCl4 (1 ml/kg BW i.p), group V treated with a single dose of curcumin (100 mg/kg BWp.o) and CCl4 (1 ml/kg BW i.p). Blood was collected from vena porta for determination ofSGPT-SGOT. The study showed the activity level of SGPT from the rats was treated byEurycoma longifolia Jack. 100 mg/kg BW and 200 mg/kg BW, Curcumin, and control groupsare 150.0±5.099 U/L; 113.6±5.508 U/L; 60.5±2.887 U/L; and 129.0±6.055 U/L respectively. Mean while the activity level of SGOT from the rats was treated by Eurycoma longifolia Jack.100 mg/ kg BW and 200 mg/ kg BW, Curcumin, and control groups are 369.4±11.165;263.0±1.803; 194.5±7.448; and 451.5±16.759 U/L respectively. The Eurycoma longifoliaJack. powder and Curcumin significantly (p < 0.05) declines two enzymes (SGPT and SGOT)than control group. The results concluded that Eurycoma longifolia Jack. powder hashepatoprotective effect.
UJI TOKSISITAS AKUT EKSTRAK ETANOL KELOPAK ROSELLA (Hibiscus sabdariffa L.) PADA TIKUS Sprague Dawley Sari, Fita; Nurkhasanah, Nurkhasanah; Bachri, Moch. Saiful
Majalah Obat Tradisional Vol 21, No 1 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.27 KB) | DOI: 10.22146/tradmedj.10673

Abstract

Rosella (Hibiscus sabdariffa L.) digunakan sebagai obat tradisional karena memiliki aktivitas antioksidan. Obat tradisional digunakan untuk terapi penyakit harus memperhatikan aspek keamanan dengan dilakukan uji toksisitas agar tidak menimbulkan efek toksik. Penelitian ini bertujuan untuk mengetahui toksisitas akut ekstrak etanol kelopak rosella aktivitas aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) dan histopatologi organ hepar pada tikus Sprague Dawley (SD). Uji toksisitas akut dilakukan dengan pemberian sekali dosis ekstrak etanol kelopak rosella secara oral dalam dosis 40, 200, 1000 dan 5000 mg/kg BB. Pengamatan toksisitas di lakukan dalam rentang 14 hari setelah pemberian. Aktivitas AST, ALT dan ALP dianalisis menggunakan kit Diasys®. Hasil penelitian menunjukkan bahwa ekstrak etanol kelopak rosella ditemukan LD50 pada 850,90 mg/kg BB, aktivitas AST, ALT, ALP tidak berbeda signifikan pada kelompok perlakuan dengan kelompok kontrol (p<0,05) dan histopatologi ditemukan beberapa perubahan struktur sel dan jaringan pada organ hepar serta efek toksik yang tertunda hingga hari ke 14 juga tidak ditemukan. Kesimpulan dari penenlitian ini adalah ditemukan efek toksik secara akut dari ekstrak etanol kelopak rosella terhadap tikus SD.
PENGARUH PEMBERIAN SUBKRONIK EKSTRAK KELOPAK BUNGA ROSELLA (Hibiscus Sabdariffa L.) TERHADAP KADAR SGPT SGOT DAN ALP Nurkhasanah, Nurkhasanah; Bachri, Moch. Saiful; Azis, Nofa Risma
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 13, No 2 (2016)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.151 KB) | DOI: 10.24071/jpsc.132192

Abstract

Roselle (Hibiscus sabdariffa L.) has been known as traditional medicinal plants. It needed to evaluate the safety of roselle extract on long-term oral administration. The research aimed to determine the savety of ethanolic extract of roselle calyx. Subchronic toxicity study of ethanolic extract of roselle calyx had been carried out on 60 Sprague Dawleys (SD) rats for 28 days. The roselle extract was administered orally every day, with doses of 50,100 and 200 mg/kgBW. There are 2 satellite groups, kept for another 14 days after the treatment in order to detect a delayed occurrence of toxic effect. Satellite group was given extract 200 mg/kgBW for 28 days and followed aquadest treatment for 14 days. At the end of experiment the blood was collected for meansuring SGPT, SGOT and ALP activity. The result showed that treatment of roselle calyx extract had no significant changes in SGPT SGOT, ALP activity. The delayed effect was not also observed. The ethanolic extract of roselle calyx didn’t show toxic effects on the liver on subchronic administration.
PENGARUH EKSTRAK AKAR SENGGANI (Melastoma affine D.Don) TERHADAP MOTILITAS DAN JUMLAH SPERMA TIKUS Bachri, Moch. Saiful; Donatus, Imono Argo; Wahyuono, Subagus
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 4, No 2 (2008)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Spermatogenesis effect of  ethanol extract senggani root (Melastoma affine D. Don) was proved. Furthermore, the extract was fractionated with ethyl acetate and was investigated toward spermatogenesis effect to explore natural product  as male  contraception. The subject of this research were five groups of thirty two fertile male Wistar rat,. Four groups were treated with ethyl acetate fraction of senggani root in gradually 500; 250; 125; and 62,5 mg/kg body weight dose. The fifth group as negative control was treated by CMC Na 0,5 %. Every groups was treated by orally administration for twenty two days period of time, once a day. In twenty third day, the rats were killed then observed toward the motility, sperm count, and testis histopathology. Results of the research indicated that there were influence toward spermatogenesis effect from ethyl acetate fraction of senggani root, especially at sperm motility and sperm count. Decrease of sperm motility after treatment was compared with negative control group. The percentage of the sperm count decreased at 500; 250 mg/kg body weight dose from ethyl acetate fraction was 49,74 % and 48,21.This decreased probably were caused by seminiferous tubules necrotic.   ABSTRAK Pemberian ekstrak etanol akar senggani (Melastoma affine D. Don) telah terbukti dapat menyebabkan berkurangnya jumlah dan motilitas sperma tikus. Berdasarkan hasil penelitian tersebut dilakukan fraksinasi ekstrak etanol akar senggani dengan etil asetat kemudian fraksi itu di uji cobakan pada tikus dengan tujuan untuk eksplorasi bahan alam yang dapat dipakai sebagai bahan kontrasepsi pria. Penelitian ini menggunakan 32 ekor tikus jantan fertil galur Wistar, umur 2,5-4 bulan dengan bobot 145-350 g dan dibagi menjadi 5 kelompok. Empat kelompok merupakan kelompok perlakuan fraksi etil asetat akar senggani dengan dosis 500; 250; 125; dan 62,5 mg/kgBB. Kelompok V merupakan kelompok kontrol negatif dengan perlakuan CMC Na 0,5 %. Semua kelompok diberi perlakuan secara per oral selama 22  hari dengan frekuensi pemberian sekali sehari. Pada hari ke-23 tikus dikorbankan dan diamati motilitas, dan jumlah atau angka sperma. Dilakukan juga pengamatan histopatologi organ testis. Hasil penelitian menunjukkan ada pengaruh pemberian fraksi etil asetat akar senggani terhadap motilitas dan jumlah (angka) sperma. Motilitas sperma menjadi berkurang pada semua dosis perlakuan jika dibandingkan dengan kelompok kontrol. Penurunan motilitas sperma terbesar pada dosis 250 mg/kgBB. Jumlah sperma juga mengalami penurunan untuk semua dosis perlakuan. Persentase penurunan jumlah sperma terbesar pada dosis 500 ; 250 mg/kgBB sebesar 49,74 % , dan 48,21 %. Penurunan ini kemungkinan diakibatkan oleh rusaknya tubulus seminiferus testis yang mengalami nekrosis
The Gastroprotective Activity of Ethanol Extract of Curcuma domestica Val. on Mice Induced Ethanol - HCl Farikha, Fitri Rahmania; Bachri, Moch. Saiful
Indonesian Journal of Cancer Chemoprevention Vol 7, No 3 (2016)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev7iss3pp74-78

Abstract

Curcuma domestica Val. (CD) is one of plant used as traditional medicine in around the world. One of the benefit of CD is to treat  peptic ulcers. The aim of preset study to prove that the ethanol extract of CD  has gastroprotective  activity. This study used 25 male mice were divided into 5 groups. Group I as a control group were given suspension of 0.5% Na-CMC, groups II, III, and IV were each given ethanol extract of CD suspense with 0.5% Na-CMC at a dose of 50 mg/kg; 100 mg/kg; 200 mg/kg respectively, and group V was given ranitidine 10 mg/kg. All groups were treated orally for 6 days, then  fasted for 24 hours, on the seventh day all mice induced 3M HCl - ethanol 60% (1:1) 0.2 ml/25 gBW. One hour later, mice were sacrificed by cervical dislocation, then dissected and taken  gastric. Then performed an ulcer scoring and the curative ratio is calculated later in the histopathologic test. Data were analyzed using Kolmogorov-Smirnov test and the Levene test and ANOVA followed by LSD test level of 95%. The results showed there was no significant difference between the control with dose 50 mg/kgBW (p>0.05) and showed significant difference between control with dose 100 mg/kgBW (p<0.05),dose of 200 mg/kgBW (p<0,05), and ranitidine 10 mg/kgBW (p<0.05). Curative ratio percentage dose of 100 mg/kgBW; 200 mg/kgBW, and ranitidine are 38.89%, 61.11% and 66.67 % respectively. The conclusion of this research, the ethanol extract of  Curcuma domestica Val at the dose 100 mg/kgBW and 200 mg/kgBW showed  have gastroprotective activity.Keywords: Gastroprotective, Curcuma domestica Val., ethanol-HCl, Gastric ulcer
Combination Effects of Moringa oleifera Leaf Ethanol Extract and Andrographis paniculata Herb on Blood Glucose Levels and Pancreas Histopathology of Diabetic Rats Induced by Streptozotocin Fatmawati, Annisa; Bachri, Moch. Saiful; Nurani, Laela Hayu
Majalah Obat Tradisional Vol 24, No 2 (2019): IN PRESS
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2639.634 KB) | DOI: 10.22146/mot.39401

Abstract

Type 2 diabetes mellitus is a non-contagious disease, can damage the glucose metabolic system in the body, and is characterized by hyperglycemic conditions. Moringa leaves (Moringa oleifera) and Andrographis herbs (Andrographis paniculata) have been studied to have antihyperglycemic activity in diabetic rats. The purpose of this study was to determine the effect of the combination of Moringa leaf ethanol extract (MLEE) and ethanol extract of Andrographis herbs (AHEE) in streptozotocin-induced rats (45 mg/kgBW). The subjects consisted of 32 rats, divided into 8 groups (1 healthy group and 7 type 2 DM groups). The study was conducted for 4 weeks by measuring blood glucose levels in pre-treatment, day 0, 14 and 28 with a single MLEE treatment dose of 300 mg/kgBW, single AHEE 300 mg/kgBW, combination of MLEE and AHEE 150+150 mg/kgBW, 200+100 mg/kgBW, 100 + 200 mg/kgBW, and gliclazide 5 mg/kgBW orally. The results of measurement of fasting blood glucose levels on day 28 showed that administration of gliclazide 5 mg/kg BW, single dose MLEE and AHEE, as well as its combination, had significant differences (p<0.05) compared to the hyperglycemic control group. Pancreatic organ histopathology features in the extract dose group showed that there was a change in the repair of insula Langerhans compared to the hyperglycemic control group which showed necrotic damage due to streptozotocin induction. Combination administration has the same antihyperglycemic effect by single dose extract in diabetic rats within 28 days, which also restore weight loss to normal.
PENGARUH EKSTRAK AKAR SENGGANI (Melastoma affine D.Don) TERHADAP MOTILITAS DAN JUMLAH SPERMA TIKUS Bachri, Moch. Saiful; Donatus, Imono Argo; Wahyuono, Subagus
Jurnal Farmasi Indonesia Vol 4, No 2 (2008)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v4i2.15

Abstract

Spermatogenesis effect of  ethanol extract senggani root (Melastoma affine D. Don) was proved. Furthermore, the extract was fractionated with ethyl acetate and was investigated toward spermatogenesis effect to explore natural product  as male  contraception. The subject of this research were five groups of thirty two fertile male Wistar rat,. Four groups were treated with ethyl acetate fraction of senggani root in gradually 500; 250; 125; and 62,5 mg/kg body weight dose. The fifth group as negative control was treated by CMC Na 0,5 %. Every groups was treated by orally administration for twenty two days period of time, once a day. In twenty third day, the rats were killed then observed toward the motility, sperm count, and testis histopathology. Results of the research indicated that there were influence toward spermatogenesis effect from ethyl acetate fraction of senggani root, especially at sperm motility and sperm count. Decrease of sperm motility after treatment was compared with negative control group. The percentage of the sperm count decreased at 500; 250 mg/kg body weight dose from ethyl acetate fraction was 49,74 % and 48,21.This decreased probably were caused by seminiferous tubules necrotic.   ABSTRAK Pemberian ekstrak etanol akar senggani (Melastoma affine D. Don) telah terbukti dapat menyebabkan berkurangnya jumlah dan motilitas sperma tikus. Berdasarkan hasil penelitian tersebut dilakukan fraksinasi ekstrak etanol akar senggani dengan etil asetat kemudian fraksi itu di uji cobakan pada tikus dengan tujuan untuk eksplorasi bahan alam yang dapat dipakai sebagai bahan kontrasepsi pria. Penelitian ini menggunakan 32 ekor tikus jantan fertil galur Wistar, umur 2,5-4 bulan dengan bobot 145-350 g dan dibagi menjadi 5 kelompok. Empat kelompok merupakan kelompok perlakuan fraksi etil asetat akar senggani dengan dosis 500; 250; 125; dan 62,5 mg/kgBB. Kelompok V merupakan kelompok kontrol negatif dengan perlakuan CMC Na 0,5 %. Semua kelompok diberi perlakuan secara per oral selama 22  hari dengan frekuensi pemberian sekali sehari. Pada hari ke-23 tikus dikorbankan dan diamati motilitas, dan jumlah atau angka sperma. Dilakukan juga pengamatan histopatologi organ testis. Hasil penelitian menunjukkan ada pengaruh pemberian fraksi etil asetat akar senggani terhadap motilitas dan jumlah (angka) sperma. Motilitas sperma menjadi berkurang pada semua dosis perlakuan jika dibandingkan dengan kelompok kontrol. Penurunan motilitas sperma terbesar pada dosis 250 mg/kgBB. Jumlah sperma juga mengalami penurunan untuk semua dosis perlakuan. Persentase penurunan jumlah sperma terbesar pada dosis 500 ; 250 mg/kgBB sebesar 49,74 % , dan 48,21 %. Penurunan ini kemungkinan diakibatkan oleh rusaknya tubulus seminiferus testis yang mengalami nekrosis