MARIA BINTANG
Departement of Biochemistry, Faculty of Mathematic and Natural Science, Bogor Agricultural University, Dramaga Campus, Bogor 16680, Indonesia

Published : 43 Documents
Articles

IDENTIFIKASI KOMPONEN KIMIA DAN AKTIVITAS ANTIOKSIDAN DALAM TANAMAN TORBANGUN (COLEUS AMBOINICUS LOUR) Suryowati, Trini; Rimbawan, Rimbawan; Damanik, Rizal M; Bintang, Maria; Handharyani, Ekowati
Jurnal Gizi dan Pangan Vol. 10 No. 3 (2015)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.092 KB) | DOI: 10.25182/jgp.2015.10.3.%p

Abstract

ABSTRACTThe objective of this study was to determine the chemical compound in leaves, stem, and root by GC-MS technique and antioxidant activity of torbangun leaves (Coleus amboinicus Lour). The torbangun leaves ethanol extract were tested to antioxidant activity assay using DPPH, and á-glucosidase inhibitory effects was measured with a spectrophotometric method. The analysis of leaves revealed the presence of Carbamic acid, monoammonium salt (CAS) Ammonium carbamate (11.73%), Hexadecanoic acid (CAS) Palmitic acid (8.35%), I-Limonene (5.92%), Heptadecene-(8)- carbonic acid-(1) (4.76%), Oxacycloheptadec-8-en-2-one (CAS) Ambrettolide (4.70%). The analysis of stem revealed the presence of Formamide (CAS) Methanamide (22.48%), 12,13- Dimethyl-2,7- dioxa 5,10 diazatricyclo [4.4.4.0(1,6)] trans -tetradecan-12 (13.22%), Hexadecanoic acid (CAS) Palmitic acid (11.51%), 2-Propanone, 1-hydroxy- (CAS) Acetol (10.14%), 9-Octadecen-1-ol, (Z)- (CAS) cis-9-Octadecen-1-ol (7.09%). The analysis of roots revealed the presence of Methanamine, N-methyl- (CAS) Dimethylamine (28.45%), Acetic acid (CAS) Ethylic acid (9.78%), 3.2-Propanone, 1-hydroxy- (CAS) Acetol (6.41%), 1-Propen-2-ol, acetate (CAS) Isopropenyl acetate (5.16%), 4.73 Phenol, 2-methoxy- (CAS) Guaiacol(4.73%). The DPPH result of torbangun leaves ethanol extract obtained by IC50 247,942 ppm and ascorbic acid standard was 1 ppm. IC50 values inhibition of á-glucosidase extract was >100 ppm and glucobay standard was 0.264 ppm. This research provided a chemical compound and the torbangun leaves ethanol extract capable of acting as antioxidant based on IC50 values.Keywords: antioxidant activity, chemical compound, Coleus amboinicus LourABSTRAKPenelitian ini bertujuan untuk mengetahui komponen senyawa kimia dalam daun, dahan, dan akar menggunakan analisis Kromatografi Gas-Spektrometri Massa (GC-MS) serta aktivitas antioksidan daun torbangun (Coleus amboinicus Lour). Daun torbangun yang diekstrak etanol dianalisis aktivitas antioksidan menggunakan test DPPH, dan daya hambat enzim á-glukosidase diukur dengan metode spektrofotometer. Hasil analisis dalam daun menunjukkan komponen kimia Carbamic acid, monoammonium salt (CAS) Ammonium carbamate (11,73%), Hexadecanoic acid (CAS) Palmitic acid (8,35%), I-Limonene (5,92%), Heptadecene-(8)-carbonic acid-(1) (4,76%), Oxacycloheptadec-8-en-2-one (CAS) Ambrettolide (4,70%). Hasil analisis dalam dahan menunjukkan komponen kimia Formamide (CAS) Methanamide (22,8%), 12,13-Dimethyl-2,7-dioxa5,10diaza tricyclo [4.4.4.0(1,6)] trans-tetradecan-12 (13,22%), Hexadecanoic acid (CAS) Palmitic acid (11,51%), 2-Propanone,1-hydroxy-(CAS) Acetol (10,14%), 9-Octadecen-1-ol, (Z)- (CAS) cis-9-Octadecen-1-ol (7,09%). Hasil analisis dalam akar menunjukkan komponen kimia Methanamine, N-methyl-(CAS) Dimethylamine (28,45%), Acetic acid (CAS) Ethylic acid (9,78%), 3.2-Propanone, 1- hydroxy- (CAS) Acetol (6,41%), 1-Propen-2-ol, acetate (CAS) Isopropenyl acetate (5,16%), 4.73 Phenol, 2-methoxy- (CAS) Guaiacol (4,73%). Hasil uji antioksidan terhadap daun torbangun dengan metode DPPH didapatkan IC50 247,942 ppm dibandingkan standar vitamin C 1 ppm. Nilai IC50 dari penghambatan enzim á-glukosidase dalam ekstrak daun torbangun >100 ppm dibandingkan dengan standar glukobay 0,264 ppm. Hasil penelitian ini menunjukkan bahwa daun torbangun yang diekstrak dengan etanol mampu berperan sebagai antioksidan berdasarkan nilai IC50.Kata kunci: aktivitas antioksidan, Coleus amboinicus Lour, komponen kimia
DETEKSI ENZIM CELLOBIOSE DEHYDROGENASE (CDH) DARI FUNGI TRAMETES VERSICOLOR Desriani, Desriani; Falah, Syamsul; Bintang, Maria; Kusumawati, Dwi Endah; Amrinola, Wiwit; Hasanah, Neneng
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

Trametes versicolor merupakan salah satu fungi pendegradasi kayu dari golongan white rot fungi yang memiliki kemampuan dalam menghasilkan enzim ekstraseluler, yaitu cellobiose dehydrogenase (CDH). Enzim CDH memiliki dua domain, yaitu domain flavin dan domain heme yang dihubungkan oleh 25 asam amino yang kaya akan serin dan treonin. Penelitian ini bertujuan mendeteksi CDH yang dihasilkan oleh T. versicolor baik secara kualitatif maupun kuantitatif. Deteksi CDH dilakukan melalui empat  tahap, yaitu: uji kualitatif enzim ekstraseluler, produksi CDH pada media cair, pengukuran aktivitas CDH menggunakan DCIP sebagai akseptor elektron dan selobiosa sebagai substrat, serta analisis bobot molekul CDH dengan elektroforesis SDS-Page. Hasil penelitian menunjukkan terbentuknya zona bening disekitar fungi setelah perlakuan congo red dan NaCl yang mengindikasikan bahwa T. versicolor menghasilkan enzim ekstraseluler. Produksi CDH dilakukan dengan menumbuhkan fungi di media YPD dan media minimal guna menginduksi produksi CDH. Pengukuran aktivitas enzim CDH belum berhasil dilakukan dan berdasarkan hasil SDS-PAGE, sebagian besar enzim CDH dari T. versicolor dihasilkan dalam bentuk domain flavin.  Kata kunci : Cellobiose dehydrogenase, SDS-Page, Trametes versicolor
Isolation of anticancer compound of Artemisia cina hairy root and its inhibition activity on cervix cancer cells. ., Aryanti; Ermayanti, Tri Muji; Mariska, Ika; Bintang, Maria
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 4, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.69 KB) | DOI: 10.14499/indonesianjpharm0iss0pp192-196

Abstract

The research of isolation of anticancer agent of A.cina hairy roots and its inhibition activity on cervix cancer cells have been conducted. Hairy roots transformed by Agrobacterium rhizogenes strains A4 and ATCC-15834 were then extracted by n-hexane and separated by column chromatography with variation of n-hexane/ethyl acetate as eluent. All samples include hexane extract and result of column chromatography tested to cervix HeLa Ohio cells with concentration of 50 μg/ml for hexane extract and 10 μg/ml for column chromatography respectively. The most active fraction was then tested by the concentration of 1 to 5 μg/ml. Confirmation of transformed root of A.cina was conducted by PCR analysis. The result of experiment shown that hexane extract of hairy root, normal root ( in vitro ), leaves of plant from green house as a control gave the inhibition value were about 84 %. The most active fraction from column chromatography was fraction E with IC50 at the concentration of 1 μg/ml and inhibition value was 95 %, the identification compound of this fraction was terpenoid group. The confirmation result showed that TL-DNA was transferred by 780 kb.Key words : anticancer, hairy root, Artemisia cina.
Delignifikasi Batang Kayu Sengon oleh Trametes versicolor Azhari, Azmi; Falah, Syamsul; Nurjannah, Laita; Suryani, Suryani; Bintang, Maria
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

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Abstract

Delignification is a lignin degradation, a preliminary process in industries that used cellulose containingsubstrates. Sengon logs are often used for the material in pulp industry because it has high levels of celluloseand low level of lignin. The aim of this study was delignification of sengon logs by using T.versicolor. Themethods used include observation growth of T.versicolor compared with Phanerochaete chrysosporium, therate of of lignin degradation (black liquor), delignification of sengon logs using T.versicolor and the chemicalassay of sengon logs before and after delignification. The results of this study showed that delignification byT.versicolor was faster compared to P.chrysosporium based on the rate of lignin degradation (black liquor).The result showed that delignification by T.versicolor at room temperature reduced lignin of sengon logs by37.31 % within 20 days. Chemical assay performed on delignified sengon wood showed decreased level ofethanol benzene, soluble extractive substances, holocellulose, and cellulose and an increase of hemicelluloselevel.
Induksi dan Karakter Pertumbuhan Kalus Triploid dari Endosperma Avokad (Persea americana Mill.) Sukmara, Edy; Sukamto, Lazarus Agus; Bintang, Maria
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

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The concentration of the Plant Growth Regulators (PGR) medium as well as endosperm viabilitydetermines the success of triploid avocado callus. This study was aimed to explore the best size of diameterfruit and the most responsive concentration for the induction of endosperm avocado callus. The studyconsisted of two phases. The first stage is to determine the best diameter size of avocado and concentrationof the culture medium that gave the fastest response to be endosperm callus. The second stage usedflow cytometer to obtain triploid of avocado endosperm callus. This study used four size of fruit groupdiameter, that A=(0.30-0.50), B=(0.51-1.00), C=(1.01-1.50) and D=(1.51-2.20) in cm. Factorial experimentwith four replications was arranged in a completely randomized design. Murashige and Skoog medium(MS) was used in this culture with two PGR that the Picloram and 2.4- D. Each with concentration :0.5, 1.0 , 2.0 and 4.0 in mg/l. The most responsive diameter fruit size was group B (0.30 – 0.50 cm) withthe fastest callus growth average was 7.67 weeks after culture (WAC). The most responsive concentrationfor endosperm callus induction was Picloram 2 mg/l, growth in 5.08 WAC. The endosperm callus growthsuccessfully t done by the inclusion of avocados embryos. The best interaction between fruits diameter andconcentration for callus avocado induction used fruits diameter A with picloram 2 mg/l. The endospermcallus were measured by using flow cytometer resulted in avocado triploid callus with region range (RN)Mean value wer 302.01, 296.60, and 298.51 respectively. while the avocado leaf control resulted in 198.82diploid plant.
Aktivitas antibakteri isolat bakteri endofit dari tanaman miana (Coleus scutellariodes [L.] Benth.) terhadap Staphylococcus aureus dan Escherichia coli Kusumawati, Dwi Endah; Pasaribu, Fachriyan Hasmi; Bintang, Maria
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

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Coleus scutellarioides [L.] Benth. known as medicinal plant which contains antidiarhea and antimicrobialcompounds. Extraction of bioactive compound from plants is not efficient because it needs a large biomass,therefore the one of appropiate ways is using endophytic bacteria. The purpose of this research was to isolateand test of antibacterial activity of endophytic bacteria from Coleus scutellarioides [L.] Benth. againsttwo pathogenic bacteria i.e Eschericia coli and Staphylococcus aureus. A total of 22 isolates of endophyticbacteria were obtained from Coleus scutellarioides [L.] Benth. Based on antibacterial activity test, 13 isolatesof endophytic bacteria were able to inhibit the growth of E. coli and 15 isolates were able to inhibit S. aureus,whereas endophytic bacteria which can inhibit both types of pathogens are 10 isolates.
Isolasi Bakteri Endofit dari Tanaman Sirih Hijau (Piper betle L.) dan Potensinya sebagai Penghasil Senyawa Antibakteri Purwanto, Ukhradiya MS; Pasaribu, Fachriyan Hasmi; Bintang, Maria
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

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Abstract

Endophytic bacteria are beneficial microorganisms that interact with host plants without causingdisruption or damage to the host. Some studies suggest that certain endophytic bacteria can produce chemicalcompounds that have an effect on health, especially endophytic bacteria isolated from medicinal plants. Greenbetel (Piper betle L.) is a medicinal plant that has been used for years and has many benefits. The purposesof this study are to isolating and screening of endophytic bacteria from green betel against four pathogenicbacteria (Staphylococcus aureus, Escherichia coli, Bacillus cereus and Salmonella enteritidis). The numberof endophytic bacteria that has been isolated are 14 isolates. Based on the screening results, three isolatesof endophytic bacteria have potential activity (characterized by the formation of inhibition zone) against S.aureus. The inhibition zone may indicate that those isolates produce compounds that have antibacterial effects. Those isolates are AS1 , BS1 and BS2. The biggest inhibition zone showed by BS1, so it can be concluded thatBS1 is the most potential isolate as a novel source of antibacterial compound.
Pupuk Hayati Berbasis Bakteri Endofit Bintang, Maria; Kusumawati, Dwi Endah
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2013
Publisher : PROSIDING SEMINAR KIMIA

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Problem of low agricultural production can be caused by a reduction in agricultural area and the presence of pests and diseases. The use of inorganic fertilizers in the long term can cause negative impacts, so we need new alternative sources of fertilizer that environmentally friendly and effective against pathogens, especially on agricultural crops.Endophytic bacteria as a biocontrol agent should be developed to be biological fertilizers because of some specific strains have been shown to produce antibacterial as well as stimulate plant’s growth. Development of endophytic bacteria as a biological fertilizer would be very beneficial, good for the environment, farmers and industry.
HIDROLISIS ENZIMATIS LIGNOSELULOSA TANDAN KOSONG KELAPA SAWIT MENJADI GULA PEREDUKSI Bintang, Maria; Gayang, Faizal; Richana, Nur
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2014
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

he processing of palm oil into crude palm oil (CPO) produced solid waste such as oil palm empty fruit bunches (OPEFB) with an average of 10 million tons / year which has not been used optimally and can pollute the environment. The main co mponents of OPEFB is cellu lose and lignin called lignocellulose waste. The content of cellulose and hemicellulose in OPEFB potential to be used as a source of reducing sugar by acid hydrolysis or enzyme. Hydrolysis of OPEFB performed using HCl followed by enzy matic hydrolysis using cellulase 0.5% and xy lanase 0.5%. The results showed a reducing sugar level produced with xylanase was 50.5 mg / L and hydrolysis with cellulase was 37.05 mg/L.
Kandungan Fitokimia, Total Fenol, dan Total Flavonoid Ekstrak Buah Harendong (Melastoma affine D. Don) Syafitri, Novilia Eka; Bintang, Maria; Falah, Syamsul
Current Biochemistry Vol 1, No 3 (2014)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/%p

Abstract

Melastoma affine plant has effect on health such as curing wound and toothache, also as an antimalarial drug. The fruit of this plant is purple and probably contain secondary metabolite compounds such as phenols and flavonoids. The total amount of both compounds may be different in unripe and ripe fruit. The aims of this research were to analyze secondary metabolite compounds and determine total phenol and total flavonoid of Melastoma affine fruit extract. The samples used in this study were unripe and ripe Melastoma affine fruits. Both of samples were extracted by three different solvents (water, 70% ethanol, and 96% ethanol) and obtained 6 extracts. Based on phytochemical test, each extract from unripe and ripe Melastoma affine fruit contained alkaloid, triterpenoid, flavonoid, phenol, and tanin. The extract with highest total phenols was 70% ethanolic extract from unripe fruit (189.56 mg/g GAE), while the extract with highest total flavonoids was 96% ethanolic extract from unripe fruit (225.50 mg/g CE). Based on this result, we conclude that unripe fruit of Melastoma affine has more total phenols and total flavonoids than ripe fruit.
Co-Authors . SURYANI Agus Setiawan Akhmad Rivai ANNA SETIADI RANTI Ardi Kapahang Aryanti . Azmi Azhari, Azmi Bawamenewi, Faoziduhu Chaidir Chaidir D.D. Sastraatmadja D.T.H. Sihombing Daniel R Monintja Daniel R. Monintja Dedy Duryadi Solichin Desriani Desriani Djihan Ryn Pratiwi, Djihan Ryn Dwi Endah Kusumawati Edy Sukmara, Edy Ekowati Handharyani Fachriyan H Pasaribu Fachriyan Hasmi Pasaribu Faizal Gayang, Faizal Hami Seno, Djarot Sasongko I MADE ARTIKA I Nyoman Suarsana Ifa Manzila Ika Mariska Iriani Setyaningsih Iwan Harjono Utama Kartiarso . Lailati, Nispi Laita Nurjannah, Laita Lazarus Agus Sukamto Leonita, Shinta LINAWATI HARDJITO M Hubeis M. Fedi A. Sondita Mas Tri Djoko Sunarno, Mas Tri Djoko MEGA SAFITHRI Mega Safithri Hasibuan, Mega Safithri Melati, Irma MIFTAHUDIN MIFTAHUDIN Misgiyarta, nFN Monisa, Fitriana S MUHAMAD SYUKUR MUHAMMAD ALFARABI Mulyasari, Mulyasari Naibaho, Frans Grovy Neneng Hasanah Ni Gusti Agung Ayu Suartini, Ni Gusti Agung Ayu Nur Richana Nurul Khumaida Panggabean, Lily Partomuan Simanjuntak Priyatno, Tri Puji PUSPITA LISDIYANTI Rahmawati, Fri Rimbawan Rimbawan Rizal M Damanik, Rizal M Ruqiah Ganda Putri Panjaitan Saadah, Susi Safira, Ukhradia Maharaniq Safira, Ukhradiya Magharaniq Saputri, Dina Dyah Sauland Sinaga SEDARNAWATI YASNI Siswa Setyahadi Suryani Suryani Syafitri, Novilia Eka Syamsul Falah Titin Kurniasih Tri Muji Ermayanti Tri Panji Tri Puji Priyatno Trini Suryowati, Trini Ukhradiya MS Purwanto, Ukhradiya MS Vinsentricia, Artha Waras Nurcholis Winangsih, Fitriani Winangsih, Fitriani Wini Trilaksani Wiwit Amrinola