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Journal : Biosaintifika: Journal of Biology

TINGKAT KEGANASAN KANKER SERVIKS PASIEN PRA-RADIASI MELALUI PEMERIKSAAN AgNORs, MIB-1 DAN Cas- 3 Kurnia, Iin; Bintari, Siti Harnina; Khaisuntaha, Mafaza
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2269

Abstract

Kanker serviks sering ditemukan di negara berkembang. Pengobatan kanker melalui radioterapi untuk mengetahui tingkat proliferasi dan mengurangi tingkat keganasan. Biomarker proliferasi dan apoptosis berupa AgNORs, MIB-1, dan Caspase 3. Namun belum dijelaskan mengenai korelasi ketiga biomarker dalam kaitannya dengan proliferasi dan apoptosis pada sel kanker serviks. Tujuan penelitian untuk mengetahui korelasi antara AgNORs, MIB-1, dan apoptosis pada kanker serviks. Penelitian observasional laboratoris menggunakan metode pewarnaan dengan menekankan kontras warna antara sitoplasma dan inti sel. Objek berupa sediaan mikroskopis dari 30 biopsi pasien kanker serviks. Pengambilan data dengan metode crocker dan blind manner. Analisis data menggunakan uji korelasi, dari ju mlah 21 pasien yang diamati menunjukkan. AgNORs dan MIB-1 memiliki angka relatif tinggi. Angka yang diperoleh ini berbanding terbalik dengan apoptosis yang relatif rendah. Korelasi antara AgNORs dengan MIB-1 menunjukkan r= 0,33 dan p= 0,15. AgNORs dengan apoptosis memiliki korelasi negatif yakni, r=-0,08 dan p= 0,73. MIB-1 dengan apoptosis memiliki korelasi negatif pula r= -0,18 dan p= 0,43. Kesimpulannya korelasi AgNORs dengan apoptosis memiliki kecenderungan lebih baik dari pada MIB-1 dengan apoptosis.Cervical cancer is often found in the developing countries. The treatment of cancer through radiotherapy was performed to determine the proliferation level and to reduce the malignancy level of cancer. The proliferation and apoptotic biomarkers were AgNORs, MIB-1, and Cas- 3. However, the correlation between the three biomarkers in relation to the proliferation and apoptosis in cervical cancer cells was not clear. The purpose of the study was to determine the correlation between AgNORs, MIB-1 and apoptosis in cervical cancer. This study was an observational research laboratory using a staining method to emphasize the color contrast between the cytoplasm and the nucleus of the cells. The microscopic preparations of the 30 patients with cervical cancer biopsies had been used as the study objects. Data was collected using the Crocker and Blind method and was then analyzed using correlation test. Data from 21 patients with AgNORs and MIB-1 showed a relatively high value. The figure obtained was inverse proportionally to the relatively low apoptotic value. The correlation between AgNORs and MIB-1 showed r = 0.33 and p = 0.15. There was negative correlation between AgNORs and apoptosis at r = -0.08 and p = 0.73. Meanwhile, between MIB-1 and apoptosis has also a negative correlation at r = -0.18 and p = 0.43. It was concluded that the correlation between AgNORs and apoptosis tended to be better than the MIB-1 and apoptosis.
ISOLASI DAN IDENTIFIKASI KHAMIR SECARA MORFOLOGI DI TANAH KEBUN WISATA PENDIDIKAN UNIVERSITAS NEGERI SEMARANG -, Jumiyati; Bintari, Siti Harnina; Mubarok, Ibnul
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2265

Abstract

Tujuan penelitian untuk mengisolasi dan mengidentifikasi khamir secara morfologi di tanah Kebun Wisata Pendidikan Unnes. Penelitian ini merupakan penelitian eksplorasi yang dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Unnes. Populasi penelitian adalah khamir di tanah Kebun Wisata Pendidikan Unnes. Sampel penelitian adalah isolat khamir yang diambil dengan teknik Purposive Sampling yaitu membagi kebun menjadi lima zona dan setiap zona diambil secara acak lima titik pengambilan kemudian dihomogenkan. Sampel dibawa ke Laboratorium untuk dilakukan isolasi, purifikasi dan identifikasi secara morfologi koloni dan sel. Simpulan penelitian ditemukan tujuh isolat khamir dan termasuk ke dalam lima genus yaitu Saccharomyces, Candida, Debaromyces, Brettanomyces dan Saccharomycodes. The purpose of the research was to isolate and to identify morphologically the individually-isolated yeasts from the soil of Biology Educational Garden Semarang State University. The exploration research was conducted in the Microbiology Laboratory Department of Biology, FMIPA Semarang State University. The yeasts were collected using purposive sampling technique in five zones and from each zone five random plots were selected to obtain the samples. Samples were isolated, purified and identified morphologically in terms of the colonies and the cells. Result revealed that seven isolated yeasts from five generas (Saccharomyces, Candida, Debaromyces, Brettanomyces, and Saccharomycodes) had been collected from the soil of Biology Educational Garden Semarang State University.
Efek Interaksi Ragi Tape dan Ragi Roti terhadap Kadar Bioetanol Ketela Pohon (Manihot Utilissima, Pohl) Varietas Mukibat Kurniawan, Tri Budi; Bintari, Siti Harnina; Susanti, R.
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3783

Abstract

Ketela pohon (Manihot utilissima, Pohl) varietas mukibat berpotensi sebagai bahan baku bioetanol. Penelitian bertujuan untuk mengetahui efek interaksi ragi tape dan ragi roti terhadap kadar bioetanol ketela pohon mukibat dan menentukan interaksi terendah yang efektif menghasilkan bioetanol yang dapat terbakar. Sampel yang digunakan adalah ketela pohon mukibat umur 7 bulan dengan berat rata-rata 500 gram. Penelitian menggunakan Rancangan Acak Lengkap pola faktorial, yaitu konsentrasi ragi tape (0%, 0,3%, 0,6% dan 0,9%) dan konsentrasi ragi roti (0%, 0,3%, 0,6% dan 0,9%) dengan 3 kali ulangan. Parameter yang diukur meliputi jumlah sel khamir (data pendukung) dan kadar bioetanol (data utama). Data kadar bioetanol yang diperoleh dianalisis menggunakan anava dua arah dan dilanjutkan uji Duncan (DMRT) pada taraf kesalahan 5%. Hasil penelitian menunjukkan ada pengaruh interaksi ragi tape dan ragi roti terhadap kadar bioetanol ketela pohon mukibat p>0,05. Konsentrasi ragi tape dan ragi roti yang tinggi menghasilkan kadar bioetanol yang tinggi. Interaksi ragi tape 0,6% dan ragi roti 0,3% (T2R1) merupakan interaksi terendah yang efektif menghasilkan kadar bioetanol yang dapat terbakar (36%). Interaksi ragi tape 0,9% dan ragi roti 0,9% (T3R3) menghasilkan kadar bioetanol tertinggi dengan rata-rata mencapai 49,8%.Cassava (Manihot utilissima, Pohl) var Mukibat is potential for bioethanol feedstock. The study aimed to determine the effects of the interaction of bread yeast and tape yeast on the concentration of mukibat cassava bioethanol and to determine the lowest effective interaction can produce flammable bioethanol. The samples used were mukibat cassava aged 7 months with an average weight of 500 grams. Research design was completely randomized design factorial, tape yeast concentration (0%, 0.3%, 0.6% and 0.9%) and the concentration of yeast bread (0%, 0.3%, 0.6% and 0.9%) with 3 replications. Parameters measured include the number of yeast cells (supporting data) and ethanol concentration (primary data). Bioethanol content data obtained were analyzed using two-way ANAVA and Duncan continued test (DMRT) at the level of 5% error. The results showed that no effect of the interaction of breads yeast and tape yeast on levels of cassava bioethanol p> 0.05. The concentration of bread yeast and tape yeast yielded in high concentration of ethanol. The interaction of yeast tape 0.6% and 0.3% bread yeast (T2R1) gave the lowest effective interaction produced 36% ethanol. The interaction of tape yeast 0.9% and 0.9% bread yeast (T3R3) resulted in the highest ethanol concentration with an average reached 49.8%.
Efek Inokulasi Bakteri Micrococcus luteus Terhadap Pertumbuhan Jamur Benang dan Kandungan Isoflavon pada Proses Pengolahan Tempe (Effect Inoculation of Micrococcus luteus to Growth of Mold and Content Isoflavone at Tempe Processing) Bintari, Siti Harnina; P, Anisa Dyah; J, Veronika Eka; R, Rivana Citra
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.43

Abstract

Tempe content of aglikon isoflavone which have potency to as anticancer compound. During processing of tempe, beside tempe mold (Rhizopus oligosporus) share other microbial namely bacterium. One of the bacterium residing at tempe ecosystem is Micrococcus luteus, having the character of gram positive and can conduct genistein biotransformation and daidzein become factor II compound (6,7,4-tri hydroksi isoflavone). Its problems how amount of R.oligosporus cell and M. luteus at processing of tempe associated is full scale of yielded tempe isoflavone. The study aimed to improve full scalely tempe aglikon isoflavone through inoculation of M.luteus bacteria. Used complete random designd, with M.luteus bacteria, as independent variable and variable depended is the amount of mold cell (R..oligosporus), amount of full scale M.luteus cell and total of isoflavone. Result of one way Anava indicate that inoculation of M.luteus bacteria do not degrade the amount of R.oligosporus cell with F call (53,28) > F tab (3,10) at significancy level 5%. Amount of M.luteus cell from 1,3 x 109 cell / gr [at] fermentation first becomes 3,4 x 106 cell/gr and decrease after day fermentation second becoming 1,75 x 106 cell/gr. Proportion of increasing isoflavone content at day fermentation 1-2 from 19,1 becoming 52,7 mg/100gr. Inoculation of M. luteus bacteria increased to count isoflavone aglicone and no trouble at growth of tempe mold and it relate to the increasing of is full scale of yielded tempe isoflavone.
Isolasi dan Identifikasi Virus Avian Influenza Subtipe H5N1 pada Unggas di Pasar Tradisional Semarang Ulum, Farikhul; Susanti, R.; Bintari, Siti Harnina
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.3914

Abstract

Meningkatnya kasus infeksi virus Avian Influenza (AI) subtipe H5N1 atau lebih dikenal dengan flu burung yang menyebabkan kematian pada manusia sangat dikhawatirkan dapat menular dari manusia ke manusia. Penelitian ini bertujuan untuk mendapatkan isolat virus Avian Influenza subtipe H5N1 pada unggas yang diperjualbelikan di pasar tradisional di Semarang. Sebanyak 55 sampel usap kloaka diambil dari unggas sehat dan belum divaksin di 6 pasar tradisional Kota Semarang. Inokulum ditumbuhkan pada telur ayam berembrio specific pathogen free (TAB-SPF) umur sembilan hari. Kemudian telur diinkubasikan selama 4 hari. Cairan alantois dipanen dan diuji kemampuannya mengaglutinasi sel darah merah. Cairan alantois yang menunjukkan aktivitas hemaglutinasi, selanjutnya diekstraksi RNA-nya dan diidentifikasi VAI subtipe H5N1 dengan metode Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) menggunakan primer spesifik H5 dan primer N1. Kemudian DNA hasil RT-PCR dianalisis dengan teknik elektroforesis. Hasil penelitian menunjukkan bahwa terdapat 4 isolat positif VAI subtipe H5N1 dengan sebaran 2 isolat dari sampel yang berasal dari pasar Mangkang, 1 isolat dari pasar Rejomulyo dan 1 isolat dari pasar Karimata. Berdasarkan hasil penelitian dan pembahasan dapat disimpulkan bahwa unggas yang diperjualbelikan di pasar tradisional di Kota Semarang ada yang terinfeksi VAI subtipe H5N1.The increasing cases of viral infection of Avian Influenza (AI) H5N1 subtype or more commonly known as bird flu that causes death in humans very feared to spread from human to human. The aim of this research was to obtain isolates of Avian Influenza virus (AIV) subtype H5N1 that marketable in traditional markets in Semarang. A total of 55 cloacal swab samples taken from healthy and unvaccinated fowl in the 6 traditional market in Semarang. Inoculum was grown in embryonated chicken eggs specific pathogen free (SPF TAB) nine days. Then the eggs were incubated for 4 days. Allantoic fluids were harvested and tested for their ability to agglutinate red blood cells. Allantoic fluid that showed hemagglutination activity, further their RNA was extracted and AIV subtype H5N1 identified with Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) method using specific primers primary H5 and N1. Then the results of RT - PCR were analyzed by electrophoresis technique. The results showed that there are 4 positive isolates with the distribution of the H5N1 subtype AIV 2 isolates samples derived from market Mangkang, 1 isolate from market Rejomulyo and 1 isolate from market Karimata. Based on the results of research and discussion, it can be concluded that the fowl that marketable in traditional markets in Semarang there were AIV infected with subtype H5N1.
The Potential of Microbial Symbionts Macrotermes gilvus Hagen Termite Gut as Degrading Agents of Cellulose in Bioethanol Production Susilowati, Dewi; Subekti, Niken; Bintari, Siti Harnina
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14965

Abstract

Water hyacinth is a potential feedstock for bioethanol production because of their high cellulose. The microbial symbionts of the Macrotermes gilvus termite’s gut have a high endoglucanase enzyme activity. This research was aimed to analyze the pH, temperature and agitation effects towards cell density, endoglucanase enzyme activity and reducing sugar, and to determine the effective optimum condition that can produce maximum reducing sugar. This research used central composite design (CCD) with the total number of run was . The independent variables were including pH (5.9, 6.4, 7.0, 7.6, 8.0), temperature (30 0C, 33 0C, 37 0C, 41 0C, 44 0C) and agitation (90 rpm,114 rpm, 150 rpm,185 rpm, 210 rpm), with six replications at central points. Parameters measured were cell density, endoglukanase enzyme activity and reducing sugar, thus analyzed by the statistical software package MINITAB 18.0. The Student’s t-test result showed the primary sequence influencing cell density as pH ˃ agitation ˃ temperature and towards endoglucanase enzyme activity and reducing sugar as pH ˃ temperature ˃ agitation, P ˂ 0.05. The maximum reducing sugar (60.13 ± 3.16 mmolL-1) was obtained at pH 6.95, temperature 37 0C and agitation 150 rpm. The results of this research can be used to explore the more potential microbial symbionts of the Macrotermes gilvus Hagen termite’s gut.