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PENGGUNAAN INTERJEKSI DALAM NOVEL NONA SEKRETARIS KARYA SUPARTO BRATA Budiarti, Sri
ADITYA - Pendidikan Bahasa dan Sastra Jawa Vol 7, No 1 (2015): ADITYA
Publisher : Pendidikan Bahasa dan Sastra Jawa

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Abstract

Abstrak: penelitian ini bertujuan: (1) mendeskripsikan bentuk interjeksi dalam novel Nona Sekretaris karya Suparto Brata; (2) mendeskripsikan fungsi interjeksi dalam novel Nona Sekretaris karya Suparto Brata, yaitu (a) bentuk primer (o, lho, ah, lo, heh, wah, la, oh, ei, ha, wo, e, hiss, huh, hee, huuuh, oo, huss, wih), (b) bentuk sekunder (ayo, buset, stop, gendheng, athoo, embuh, hebat, edan, wahdhuh, toblas, emoh, adhuh, kapok, bodho, astagfirullah, bangsat), (c) bentuk pengulangan kata (ah-ah, apik-apik, ala-la-la, his-his, ala-la-la, e-e). Bentuk interjeksi memiliki fungsi berbeda-beda: (1) (a) interjeksi keheranan, (b) interjeksi kekaguman, (c) interjeksi kekesalan, (d) interjeksi panggilan, (e) interjeksi mengeluh, (f) interjeksi kekagetan, (g) interjeksi pujian, (h) interjeksi kemarahan, (i) interjeksi kesedihan, (j) interjeksi tidak senang, (k) interjeksi kesenangan, (l) interjeksi harapan, (m) interjeksi kekecewaan, (n) interjeksi harapan, (o) interjeksi ajakan, (p) interjeksi kesakitan, (q) interjeksi makian.   Kata kunci: interjeksi, novel Nona Sekretaris
Metagenomic Analysis of Bacteria Phylum Firmicutes and Bacteroidetes in Women with Type 2 Diabetes Zulhaerati, Zulhaerati; Budiarti, Sri; Astuti, Rika Indri
HAYATI Journal of Biosciences Vol. 26 No. 3 (2019): July 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.115 KB) | DOI: 10.4308/hjb.26.3.%x

Abstract

Diabetes mellitus (DM) is a serious health problem in Indonesia. Type 2 diabetes is a metabolic disease which primarily caused by obesity-linked insulin resistance. There is a link between insulin resistance and bacterial populations in the gut. Therefore, the aims of this study was to analyze composition of bacteria belong to the phylum Firmicutes and Bacteroidetes in women with type 2 diabetes and non-diabetic persons as control. The study included five female adults type 2 diabetes patients and five healthy controls. The bacteria composition was analyzed for abundance by denaturing gradient gel electrophoresis (DGGE). Metagenomic analysis based on 16S rRNA gene was represented by 12 DGGE bands. The twelve respective bands showed the similarity ranging from 77 up to 98%. Diversity of microbial composition in women type 2 diabetes was represented by five DGGE bands are Bacteroides fragilis, Bacteroides vulgatus, uncultured Bacteroides sp. clone Lb4eF4, uncultured bacterium clone 16sms90-5g05, and uncultured bacterium clone Malaga 1F14. Four DGGE bands in women healthy controls are closely related to uncultured bacterium isolate DGGE gel band Eub42, uncultured bacterium isolate DGGE gel band K115, uncultured bacterium clone HFV04255, and Enterococcus sp. the finding shows bacterial species belong to the phylum Bacteroidetes was mostly found in women diabetic group, while those belong to the phylum Firmicutes was mostly found in healthy controls.
Potret Kebijakan Pengendalian Demam Berdarah Dengue di Kabupaten Indramayu Peranginangin, Henri; Hasim, Hasim; Pramudya, Bambang; Budiarti, Sri
Jurnal Kesehatan Masyarakat Nasional Vol. 4 No. 3 Desember 2009
Publisher : Faculty of Public Health Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.484 KB) | DOI: 10.21109/kesmas.v4i3.185

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Penyakit demam berdarah dengue (DBD) masih menjadi masalah di Kabupaten Indramayu. Penelitian ini bertujuan mengidentifikasi elemen-elemen priori-tas kebijakan pengendalian penyakit DBD di Kabupaten Indramayu menurut pakar berdasarkan metode Analytical Hierarchy Process (AHP). Hasil peneliti-an menunjukkan “strategi utama” pengendalian penyakit DBD di Kabupaten Indramayu adalah peningkatan kesehatan lingkungan permukiman; “aktor utama pengendalian” ialah Pemerintah Kabupaten Indramayu; “faktor utama pengendalian” adalah lingkungan; “tujuan utama pengendalian” ialah Kabupaten Indramayu bebas penyakit DBD; dan “kriteria utama pengendalian” adalah jumlah dan mutu sumber daya manusia. Agar implementasi strategi pengendali-an itu efektif maka Pemerintah Kabupaten Indramayu perlu meningkatkan kerja sama lintas program dan sektoral; dukungan teknologi, dana dan sarana pendidikan kesehatan lingkungan; mutu layanan Puskesmas; dan pengembangan tim pengendalian penyakit DBD dari tingkat Kabupaten sampai Desa/Kelurahan.Kata kunci : Pengendalian, DBD, AHP, kesehatan, lingkungan.AbstractDengue haemorrhagic fever (DHF) still becomes health problem in Indramayu district. The objective of this research is to identify the priority elements of DHF controlling policy in Indramayu district according to 35 experts using analytical hierarchy process (AHP). The result of the research provides information that the “main strategy” of DHF controlling is the improvement of healthy living environment; the “main actor” is the Government of Indramayu district; the “main factor” is the environment; the “main objective” is zero DHF in Indramayu district; and the “main criteria” is the quantity and quality of human resources. Based on this data, in order to implement the main strategy effectively, the Government of Indramayu district should increase the interprogram and inter-institu-tional cooperation; provide technological, funding, and facilities of environment health education supports; increase the quality of Puskesmas services and develop the DHF controlling team in all administrative level. Key words : Controlling, DHF, AHP, health, environment.
Salmonella population in waste water treatment installation (IPAK) at Pulo Gebang DKI Jakarta Budiarti, Sri; Rusmana, Iman
Medical Journal of Indonesia Vol 7 (1998): Supplement 1
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

[no abstract available]
Lipase Activity of Endophytic Actinobacteria from Medicinal Plants Lestari, Yulin; Wirawan, Budi; Budiarti, Sri; Rahminiwati, Min
HAYATI Journal of Biosciences Vol. 25 No. 1 (2018): January 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.91 KB) | DOI: 10.4308/hjb.25.1.1

Abstract

Endophytic bacteria are known to reside within host plant tissue without giving a harmfull effect. The endophytes may play an important role, as they may produce similar bioactive compounds as produced by the host plant. Various medicinal plants have long been used to cure diseases. Traditionally, leaves extract of Guazuma ulmifolia, Psidium guajava, or the rhizome of Curcuma xanthorrhiza can be used to treat disease, e.g. hyperlipidemic. The mechanism can be through lipase activity, where the lipase catalyzes the hydrolysis of triacylglycerol to fatty acids and acylglycerol. The objective of this research was to assess potency of endophytic bacteria as anti-hyperlipidemic compounds producer through their lipase activity. Sixty nine endophytic bacteria which comprised of 22, 27 and 20 isolates were isolated from the leaves of G. ulmifolia, P. guajava, and the rhizome of C. xanthorrhiza, respectively. Eight out of the 69 isolates showed lipase activity, and the two selected isolates, i.e. DPG 3(2) and AJB 4(4) were considered as good lipase producers. The highest specific lipase activity of DPG 3(2) isolate was observed for 0.874 units per mg at 38 h, whereas AJB 4(4) isolates had the specific lipase activity at 1.139 units per mg after 72 h observation. These data indicate that the two selected isolates have the potency as antihyperlipidemic compounds producer through their lipase activity.
Peran Ekstrak Etanol Kulit Manggis (Garcinia mangostana L.) Dalam Menginduksi Apoptosis Sel Kanker Lidah Manusia Sp-C1 In Vitro -, SUPRIATNO; SUSANTO, HENDRI; BUDIARTI, SRI
Indonesian Journal of Cancer Vol 7, No 4 (2013): Oct - Dec 2013
Publisher : "Dharmais" Cancer Center Hospital

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Abstract

Sel kanker lidah mempunyai karakteristik pertumbuhan sel yang cepat, invasi, dan metastasis yang tinggi. Penatalaksanaan untuk kanker primer, metastasis, dan residif masih menunjukkan hasil yang belum memuaskan sehingga dipikirkan untuk mengombinasi dengan terapi pengobatan alternatif menggunakan bahan alam kulit manggis (Garcinia mangostana L.). Tujuan penelitian menguji induksi apoptosis sel kanker lidah Sp-C1 menggunakan ekstrak etanol kulit manggis in vitro. Induksi apoptosis sel pasca-perlakuan ekstrak etanol kulit manggis konsentrasi 0, 2,5, 5, 7,5, 10, dan 12,5 ?g/ml dilakukan menggunakan uji kolorimetrik caspase-3 dan -9 (DVED-pNA dan LEHD-pNA). Data dianalisis menggunakan Anova satu jalur, dilanjutkan dengan uji Post-hoc LSD dengan derajat kemaknaan 95%. Hasil penelitian menunjukkan ekstrak etanol kulit manggis konsentrasi 12,5 ?g/ml menginduksi apoptosis sel kanker lidah manusia Sp-C1 melalui aktivitas proteolitik caspase-3 dan caspase-9 (P=0,001). Peningkatan kelipatan aktivitas proteolitik caspase-3 dan -9 diketahui sebesar 1,39 dan 2,15 kali lipat. Kesimpulannya, ekstrak etanol kulit manggis dapat menginduksi apoptosis sel kanker lidah manusia Sp-C1.Kata Kunci: sel kanker lidah Sp-C1, kulit manggis, apoptosis, caspase-3 dan -9. 
Characterization of Bacteriophage Specific to Bacillus pumilus from Ciapus River in Bogor, West Java, Indonesia KUSMIATUN, ANIK; RUSMANA, IMAN; BUDIARTI, SRI
HAYATI Journal of Biosciences Vol. 22 No. 1 (2015): January 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2344.168 KB) | DOI: 10.4308/hjb.22.1.27

Abstract

Bacillus pumilus is a spore-forming bacteria that is rod-shaped, gram positive, and aerobic. B. pumilus produced pumilacidins, known to have toxic effects on epithelial cells. Antibiotics were usually used to treat the disease caused by bacteria. Antibiotic typing test of B. pumilus indigenous from sewage water showed that this isolate was resistant to ampicillin and clindamycin. An alternative way was by application of bacteriophages as biocontrol agents to reduce B. pumilus in environment. The aim of this study were to isolate and characterize B. pumilus bacteriophage isolated from Ciapus River in Bogor, West Java. Bacteriophages infecting B. pumilus were isolated from river water using the double agar overlay method. Phages were defined by plaque morphology, structure, host range, and characteristic of molecular weight protein phage. Phage FBa1, FBa2, and FBa3 had narrow host range and they were specific for infecting B. pumilus. Electron microscope observation showed that phage FBa1 had icosahedral head without tail (166.67 nm in diameter), so it is called phage-like particles. Characterization of phage FBa1 by SDS-PAGE showed five proteins band. Molecular weight of FBa1 proteins was 70.9, 54.9, 33.8, 28.3, and 21.4 kDa.
Phage FR38 Treatment on Sprague Dawley Rat Inferred from Blood Parameters and Organ Systems SARTIKA, DEWI; BUDIARTI, SRI; SUDARWANTO, MIRNAWATI
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.476 KB) | DOI: 10.4308/hjb.19.3.131

Abstract

The ability of phage FR38 to lysis indigenous Salmonella P38 from feces of diarrheal patient has been studied. However, effects of phage FR38 on organ system were not revealed as yet. This study was conducted to observe the effect of phage FR38 on blood chemistry, kidney functions, and liver functions. Twelve Sprague-Dawley rats were used as a model for this study that were divided into two groups; (i) control and (ii) treated group with phage FR38. For treated phage group, each rat was administered by 5 ml/kg bw of 1.59·107 pfu/ml of phage intragastric. The blood parameters were analysed on day 16. The results revealed that body and organs weight, erythrocyte, hematocrit, hemoglobin, leukocyte, total protein, creatinine, SGOT, and SGPT of phage treatment rats were not significantly different with the control rats on day 16 (P > 0.05). Therefore, this study showed was no effect of phage FR38 on body weight, blood chemistry, kidney and liver functions of the rat (P > 0.05).
Association of Sexual Maturation and Body Size of Arfak Children KAWULUR, ELDA IRMA JEANNE JOICE; SURYOBROTO, BAMBANG; BUDIARTI, SRI; HARTANA, ALEX
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.56 KB) | DOI: 10.4308/hjb.19.3.124

Abstract

Gonad maturation in pubertal girls and boys is accompanied with somatic growth spurt, changes in quantity and distribution of body fat (BF), development of secondary sex characters, and relevant physiological events. Menarche (first event of menstruation) and spermarche (first event of nocturnal sperm emission) are usually used as indicators of gonad maturation. We found that median age at menarche of Arfak girls in Manokwari, West Papua is 12.2 years, while median age at spermarche of boys is 13.6 years. A possible factor causing young age at menarche is due to adaptation to unstable environmental conditions because of high risk of mortality by malaria disease during childhood. The events of menarche and spermarche achieved one year after the peak body height (BH) velocity, and just before or at the same time with the time of maximum growth rate of body weight (BW), body mass index (BMI), and BF. The average BMI of Arfak girls was big at 21.9 kg/m2 at the time of their menarche. Bigger average BMI might be caused by prepubertal slowing down of BH growth compare to growth of BW whichis still increasing. Girls accumulate BF before puberty to be used as an energy reserve for the occurrence of menarche. At the time of development of secondary sexual characters girls use the fat reserve so it decline sharply after puberty. In boys, growth rate of BF was stopped at 11 years old, and then growing negatively presumably because boys use fat mass for the occurence of spemarche. BF growth rate reached the lowest point at the age 16 years old, and then increase linearly with age through adolescence until adulthood at age 23 years old.
Growth of body size (body height and body weight) and somatotype in 363 girls and 299 boys aged 4 to 20 years of ethnic Javanese lived in Magelang Regency Indonesia were studied cross-sectionally. Over half of them were categorized in the well-off family, therefore underweight or underfat prevalence in our subjects was low (14.3%) but overweight and obesity prevalence was also low (14%). They were shorter and lighter than reference children from U.S., Japan and Yogyakarta but they improved when WIDIYANI, TETRI; SURYOBROTO, BAMBANG; BUDIARTI, SRI; HARTANA, ALEX
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.182

Abstract

Growth of body size (body height and body weight) and somatotype in 363 girls and 299 boys aged 4 to 20 years of ethnic Javanese lived in Magelang Regency Indonesia were studied cross-sectionally. Over half of them were categorized in the well-off family, therefore underweight or underfat prevalence in our subjects was low (14.3%) but overweight and obesity prevalence was also low (14%). They were shorter and lighter than reference children from U.S., Japan and Yogyakarta but they improved when compared with those of the same ethnic of Bantul and with the different ethnic of rural India. There was a clear age-related change of their somatotype. At age 4 years, the physique of children subjects in both sexes is found to be mesomorph-endomorph. Thereafter it is transformed into ectomorphic-endomorph in girls and to mesomorph-ectomorph in boys at the age of 20 years. In girl subjects, the onset of puberty was characterized by an acceleration of endomorphy component at age 8 years. While in our boys it was characterized by an acceleration of ectomorphy since age 9 years. The different growth pattern of somatotype components showed that the use of BMI as an indicator of fatness in children should be reassessed.