Agung Budiyanto
Bagian Reproduksi dan Kebidanan Fakultas Kedokteran Hewan Universitas Gadjah Mada Yogyakarta

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GANGGUAN REPRODUKSI SAPI BALI PADA POLA PEMELIHARAAN SEMI INTENSIF DI DAERAH SISTEM INTEGRASI SAPI - KELAPA SAWIT Budiyanto, Agung; Tophianong, Tarsisius Considus; Triguntoro, .; Dewi, Henny Kusuma
Acta VETERINARIA Indonesiana Vol. 4 No. 1 (2016): Januari 2016
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.12 KB) | DOI: 10.29244/avi.4.1.14-18

Abstract

Pemeliharaan sapi Bali di Kotamadya Bengkulu dengan sistem Sistem Integrasi Sapi - Kelapa Sawit (SISKA) sudah berjalan beberapa tahun. Salah satu parameter keberhasilan program ini adalah performa reproduksi sapi Bali. Performa reproduksi sapi Bali menggambarkan kualitas dari sistem manajemen pemeliharaan yang telah dilakukan. Kajian performa reproduksi sapi Bali tersebut sudah dilakukan dengan pemeriksaan reproduksi secara per rektal dan analisa data recording peternak dan petugas. Tujuan utama dari program manajemen reproduksi adalah mendapatkan produksi yang optimal dan keuntungan yang maksimal. Efisiensi reproduksi menentukan produktivitas, profitabilitas dan keberlanjutan dari setiap usaha peternakan. Adanya gangguan reproduksi menyebabkan inefisiensi reproduksi. Kondisi ini akan menyebabkan kerugian ekonomi. Tujuan penelitian ini adalah untuk mengetahui kondisi gangguan reproduksi dan respon kesembuhannya. Sebanyak 333 ekor sapi Bali betina dengan umur minimal 2 tahun dilakukan pemeriksaan reproduksi. Metode penelitian dilakukan melalui beberapa tahap yaitu anamnesa, pemeriksaan klinis dan pemeriksaan reproduksi secara per rektal. Penanganan gangguan reproduksi dinyatakan sembuh apabila timbulnya respon klinis berupa estrus. Data yang diperoleh kemudian dicatat dan dianalisa secara deskriptif. Berdasarkan hasil pemeriksaan diketahui bahwa 193 (57,95 %) sapi betina mengalami gangguan reproduksi yang meliputi delayed pubertas, hipofungsi ovarium, metritis, endometritis dan anestrus postpartum. Sedangkan sebanyak 80 (41,45 %) sapi sudah menunjukan gejala estrus. Adanya interaksi yang kompleks antara faktor lingkungan atau manajemen (nutrisi), respon individual, jenis gangguan reproduksi dan derajat keparahan gangguan reproduksi akan menimbulkan respon kesembuhan yang bervariasi dari setiap penanganan gangguan reproduksi.Kata kunci: gangguan reproduksi, sapi Bali, estrus, Bengkulu. (Bali Cattle Reproductive Disorders with Semi Intensive Management in The Area of Cattle - Oil Palm Integration System)The maintenance of Bali cattles in Bengkulu regency with cattle - oil palm integration system (SISKA) has been running several years. The one parameters of the success this program is the reproductive performance of Bali cattle. Bali cattle reproductive performance describe the quality of the maintenance management system that has been done. Bali cattle reproductive performance study has been conducted with reproductive rectal examination and analysis of the data recording breeders and farmer. The main purpose of the reproductive management program was getting the optimal production and maximum benefit. Reproductive efficiency determines the productivity, profitability and sustainability of each farm. The inefficiency reproductive was caused by existence of reproductive disorders. These conditions cause economic losses. The purpose of this study was to determine the condition of reproductive disorders and recovery response. A total of 333 cows Bali females with at least 2 years of age has been reproductive examination. The research methods were done through classification for several stages, anamnesis, clinical examination and reproductive examination by rectally palpation. Treatment of reproductive disorders declared cured if the onset of clinical response in the form of estrus. The data obtained then were recorded and analyzed descriptively. Based on the results of the examination reported that 193 (57.95%) of female Bali cattles experiencing reproductive disorders which include delayed puberty, ovarian hypofunction, metritis, endometritis and postpartum anestrus. While as many as 80 (41.45%) of female Bali cattles were showing signs of estrus. The existence of complex interactions between environmental factors or management (nutrition), individual responses, the type and severity of reproductive disorders were affected of varies healing response from each treatment of reproductive disorders.Keywords: reproductive disorders, bali cattle, oestrus, Bengkulu.
TINGKAT KERUSAKAN DNA SPERMATOZOA MEMENGARUHI PROFIL PROTEIN SPERMATOZOA PADA SEMEN BEKU SAPI BRAHMAN (LEVEL OF SPERMATOZOA DNA DAMAGES AFFECTS SPERMATOZOA PROTEIN PROFILES IN BRAHMAN BULLS FROZEN SEMEN) Priyanto, Langgeng; Budiyanto, Agung; Kusumawati, Asmarani; Kurniasih, Kurniasih
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.73 KB) | DOI: 10.19087/jveteriner.2018.19.4.512

Abstract

The purpose of this study was to investigate the relationship between spermatozoa DNA damages with spermatozoa protein profiles of after freezing. The rate of spermatozoa DNA damages was measured by Sperm-Bos-Halomax from two Brahman bull straw samples and the spermatozoa protein was isolated by separating the upper and lower fractions of the centrifugation results. The protein profiles were then analyzed using SDS PAGE with a 12.5% separating gel concentration. The data obtained were analyzed descriptively by comparing level of spermatozoa DNA damages with spermatozoa protein profiles of upper and lower fractions. The results of the analysis showed that in the upper fractions at 37.11% level of spermatozoa damages, one protein band (29 kDa) and at 10.66% level of DNA spermatozoa damages 9 protein bands (128 kDa, 110, 91, 55, 44, 29, 27, 25 and 20 kDa) were found, respectively. Meanwhile, in the lower fractions of frozen semen, at 37.11% level of spermatozoa DNA damages 4 protein bands (105, 82, 56 and 25 kDa), and at 10.66% level of spermatozoa DNA damages 8 protein bands (109, 95, 79, 69, 50, 44, 24 and 18 kDa) were found, respectively. It can be concluded that there are differences in the spermatozoa protein profiles between different levels of spermatozoa damages. 
OPTIMALISASI PEMBEKUAN SPERMA LIMBAH KAUDA EPIDIDIMIS KAMBING LOKAL DENGAN METODE BERTAHAP DAN STABILISASI Dalimunthe, Naela Wanda Yusria; Ridlo, M. Rosyid; Budiyanto, Agung
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (11263.13 KB) | DOI: 10.22146/jsv.34663

Abstract

Buck slaugthering produce waste such as testicles including epididymis which contain fertile sperm. Utilization of cauda epididymis as the sources of sperm for producing goat frozen sperm was not reported yet. The aims of this study were improving the frozen-thawed sperm using stabilization and multistep methods which recovered from the waste of buck slaughtering as the source of sperma. Cauda epididymis spermatozoa which was washed then diluted using extender 1 (Tris-citrate-antibiotics) and extender 2 (extender 1- glycerol-egg yolk). The extender 2 addition was performed by single or multistep methods then freezed. Modification in the pre freezing proces were performed by comparing the conventional equilibration and stabilization methods. The sperm suspension was incubated in 4°C for 2 hours after filling-sealing into straws on the equilibration group whether the stabilization group was cooled in tube 15 mL. All cooled straws from both groups were placed 4 cm horizontally on liquid nitrogen surface for 10 minutes and then plunged into liquid nitrogen for storage. The evaluation of motility parameters such as pattern of the movement and motility percentation were done followed the standard methodology. The student t-test, correlation and one-way ANOVA were used for data analysis with P<0.05. The results showed that multistep dilution method could increase the motility (25.0 ± 1.8 %) compared with single step (18.3 ± 1.7 %). Pre freezing method with stabilization also resulted higher motility (24.2 ± 2.0 %) than equilibration method (17.5 ± 2.8 %). The pattern of the movement were not different between all methods and its combination. The multistep dilution method and stabilization cooling method as well as its combination seems could increase the quality of frozen-thawed cauda epididymis spermatpzoa of local buck.
TINGKAT MATURASI IN VITRO PADA OOSIT SAPI SILANGAN SIMMENTAL PERANAKAN ONGOLE DAN LIMOUSIN PERANAKAN ONGOLE Parera, Hermilinda; Budiyanto, Agung
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.529 KB) | DOI: 10.21157/j.ked.hewan.v9i2.2821

Abstract

Penelitian ini bertujuan mengetahui tingkat maturasi oosit sapi Simmental Peranakan Ongole (SimPO) dan Limousin Peranakan Ongole (LimPO) secara in vitro. Oosit dari Ovarium sapi yang berasal dari rumah potong hewan (RPH), dikelompokkan berdasarkan jenis sapi PO (kontrol), SimPO dan LimPO. Oosit diaspirasi dari ovarium dengan syringe 5 ml dan jarum 18 G. Oosit dengan kualitas A dan B yang digunakan dalam penelitian ini. Oosit dikultur dalam media maturasi TCM 199 100 µl drop dan dilapisi minyak mineral, diinkubasi pada suhu 38,5° C, CO2 5% dan kelembaban 95% selama 24 jam. Tingkat maturasi in vitro ditentukan dengan pewarnaan aceto orcein 1% untuk melihat tahapan maturasi dengan adanya perubahan konfigurasi kromosom dan membran inti berupa germinal vesicle, germinal vesicle breakdown, metafase I, anafase/telofase I, dan metafase II. Hasil penelitian menunjukkan tingkat maturasi in vitro oosit yang mencapai metafase II dari oosit sapi PO lebih tinggi secara signifikan dibandingkan sapi SimPO dan LimPO.
REVIEW OF THE ARTIFICIAL INSEMINATION RESULT BASED ON ANESTRUS POST INSEMINATION IN BALI CATTLE HERDS AT THE REGENCY OF SIKKA, EAST NUSA TENGGARA Topianong, Tarsisius Considus; Budiyanto, Agung; Setyawan, Erif Maha Nugraha
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.101 KB) | DOI: 10.22146/jsv.5422

Abstract

Anestrus is one of indirect clinical signs of cattle pregnancy. Based on the present of sufficient progesterone on the blood,  the cattle with pregnancy condition will not show the signs of estrus.  The success of artificial insemination (AI) is the occurrence of fertilization followed by pregnancy and parturition. This involves a complex relationship between semen quality and oocytes, estrus detection, AI punctuality, inseminator and farmers ability. Artificial insemination should be performed at the relative optimum time to ovulation. Estrus detection is an important factor to determine the time of AI. Estrus Detection of Bali cattles of the farmer is often difficult observed or not observed, especially in semi-intensive system maintenance. From this review it can be seen that there is the different proportion of inseminators on the incidence of estrus after AI. However, determination of  the role of inseminator on the success of AI and  then the other factors that affect the success of the AI should be under ideal conditions. Straw from the different bulls breed gave the same of AI result. The estimate of pregnancy rate of 80 cows after AI is 55 %.  Estrus detection on 21st days after AI can be used as a detection method for early diagnosis of pregnancy, especially for the  farmers who did not have any ability or authority to perform  medical acts of reproduction which is only owned by a veterinarian. Observation, recording and evaluation of the implementation of the AI should be made until the end of pregnancy  followed by the process of parturition.
MOLECULAR EXPRESSION OF WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER 4 GENE USING ESCHERICHIA COLI BL21 Sitasiwi, Agung Janika; Artama, Wayan Tunas; Budiyanto, Agung; Dharmana, Edy
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.407 KB) | DOI: 10.21157/j.ked.hewan.v11i1.5891

Abstract

This research was conducted to find out the Wnt4 recombinant proteins which expressed by Escherichia coli (E. coli) BL21 carrying the recombinant DNA wnt4 (E. coli transformation). Research materials were E. coli BL21 transformation and E. coli BL21 non-transformation (negative control). The expression of recombinant protein was conducted by culturing E. coli for 24 hours in Luria-Bertani (LB) media with isopropyl ?-D-1-thiogalactopyranoside (IPTG) induction. Recombinant protein was isolated by sonication of pellet bacteria. Protein analysis performed by 15% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that recombinant protein with a molecular weight of 33 kDa has been expressed by E. coli BL21 transformation successfully. 
KERUSAKAN DEOXYRIBONUCLEIC ACID (DNA) SPERMATOZOA MEMENGARUHI TINGKAT KEBUNTINGAN SAPI BRAHMAN (DAMAGE TO DEOXYRIBONUCLEIC ACID (DNA) SPERMATOZOA AFFECTING THE LEVEL OF PREGNANCY IN BRAHMAN CATTLE) Priyanto, Langgeng; Budiyanto, Agung; Kusumawati, Asmarani; Kurniasih, Kurniasih
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.284 KB) | DOI: 10.19087/jveteriner.2019.20.1.119

Abstract

The relationship among of sperm DNA damage in cows with pregnancy rates has not been widely studied. The purpose of this study to determine the relationship of sperm DNA damage with pregnancy rate on Brahman cows. The sperm DNA damage rate was measured by Sperm-BosHalomax® from 2 samples of male Brahman bull straw (40002 and 40885) and pregnancy rate was measured from the success rate of artificial insemination. In 14 female Brahman cows divided into two groups. One group of 7 in the artificial insemination with 40002 males with 37.11% sperm DNA damage and one in artificial insemination with 40885 with 10.65% sperm DNA damage. The data obtained were analyzed descriptively by comparing sperm DNA damage with pregnancy rate.&nbsp;The results showed that at 37.11% sperm DNA damage level was found pregnancy rate 57.11% with ultrasound on 30 day and pregnancy rate 42.80% with ultrasound to 45 day. Result of research on sperm DNA damage level of 10.66% found pregnancy rate 57.11% with ultrasound to 30 day and level pregnancy 57.11% with ultrasound 45 days. The results of this study have concluded that there is a difference in the rate of sperm DNA damage with pregnancy rate in Brahman cows. The sperm DNA damage has an effect on pregnancy rate on Brahman cows.&nbsp;&nbsp;
KORELASI BERAT BADAN DAN UMUR SAPI TERHADAP BERAT, VOLUME CAIRAN DAN KONSENTRASI PROSTAGLANDIN F2α PADA VESIKULA SEMINALIS Dalimunthe, Naela Wanda Yusria; Budiyanto, Agung; Setyowati, Erna Prawita; Wijayanti, Agustina Dwi
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (756.727 KB) | DOI: 10.22146/jsv.29291

Abstract

Seminal vesicles were collected from 60 heads of Bulls which butchered in slaughter house (RPH) Yogyakarta. The aims of this study are knowing the relationship between body weight, age, fluids volume and concentration of prostaglandin F2 ? (PGF2?) in seminal vesicle of beef cattle. Those seminal vesicles were gathered from bulls which recorded its body weight and age then measured its seminal vesicles for weight, fluids volume and PGF2? levels. The PGF2? level was measured by Enzyme-Linked Immunosorbent Assay. Statistical analysis was performed using one way ? analysis of varian, regression and correlation with P<0.05. Body weight of bulls showed positive correlation with the weight of seminal vesicle and its fluids volume. However, PGF2? levels were not correlated with the body weight of cattle. Weight of seminal vesicles also exhibited positive correlation with volume of vesicle fluids but no correlation with PGF2? levels. Based on the age of bulls, there were no correlation withthe weight of seminal vesicles, seminal fluids volume and PGF2? levels. Those result indicated that the weight and fluids volume were affected by the body weight of bulls altough the PGF2? levels have a standard of developmentwhich seems affected by other factors such as concentration of androgen hormone.
The Effects of Reciprocal Teaching Learning Strategy and Self efficacy on Learning Outcomes of Early Childhood (AUD) Mathematical Logic Erwanto, Erwanto; Maryatmi, Anastasia Sri; Budiyanto, Agung
Al-Jabar: Jurnal Pendidikan Matematika Vol 9, No 1 (2018): Al-Jabar: Jurnal Pendidikan Matematika
Publisher : Raden Intan State Islamic University of Lampung, INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/ajpm.v9i1.2567

Abstract

The purpose of this research is to learn about the influence of Reciprocal Teaching and Self Efficacy on learning outcomes of AUD mathematical logic. This research is experimental research designed using treatment by level 2 × 2. The research method used is a variance analysis of two different cell paths. The results of this research are: (1) learning outcomes of students using Reciprocal Teaching strategy is higher than Ekspository strategy, (2) there is an interaction influence between learning strategy and self efficacy on learning outcomes of AUD mathematical logic, (3) there is an influence of self efficacy on learning outcomes of AUD mathematical logic. 
UJI ORGANOLEPTIK NUGGET DAGING DADA ITIK AFKIR DENGAN JENISDAN DOSIS TEPUNG YANG BERBEDA Rahmawati, Nurina; Budiyanto, Agung
Jurnal Ilmiah Fillia Cendekia Vol 2 No 1 (2017): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One of the resilience and diversification program of food were supplying and using local meat. Duck’s meat was soource of highly animal protein, but less of idea to process that meats. Nuggets was the produst processed meat that well liked many people. So expcted an be increase the economic of society. This research conducted at the campus of the Islamic University Kadiri on 29 april 2015 and was herped by 30 panelist. The purpose of this study to determine the species and levels or the different flour in nuggets ducks so that consumers prefer. The data obtained were analized using a completely randomized design analysis and if there is a noticeable difference then proceed to test LSD. The result showed that the species and levels or the different flour in nuggets ducks gave highly significant (P&lt;0,01) for organoleptic tests include color, aroma, taste and texture. The conclusion of this research was the panelis liked the use of &nbsp;20% level of flour in nuggets ducks