Darmawi D
Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh

Published : 6 Documents
Articles

Found 6 Documents
Search

GOBLET CELLS PROLIFERATION OF DUODENUM, JEJUNUM, AND ILEUM OF LAYING HENS IMMUNIZED WITH PROTEIN OF EXCRETORY-SECRETORY OF ASCARIDIA GALLI Balqis, Ummu; Tiuria, Risa; Priosoeryanto, Bambang Pontjo; D, Darmawi
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3129

Abstract

This research was conducted in order to examine the goblet cells proliferation in duodenum, jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridiagalli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380µg of ES andfour hours later was challenged with 4000 L2. All treatments were given orally using stainless steelcanule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on theduodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did notsignificantly on the ileum. From this result we suggested that ES would beneficial in the strengthen thehost?s defence mechanisms in the intestinal mucosa.Keywords: Ascaridia galli, excretory/secretory, goblet cells
RESPON ANTIBODI SERUM AYAM BREAKEL SILVER TERHADAP VAKSIN AVIAN INFLUENZA d, Darmawi; Hambal, Muhammad
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i2.357

Abstract

Tujuan penelitian ini adalah menguji imunogenitas dari vaksin komersial Avian Influenza (AI) berdasarkan respon imunitas humoral ayam petelur terhadap AI. Sebanyak 20 ekor ayam petelur jenis breakel silver dibagi ke dalam dua kelompok masing-masing berjumlah 10 ekor. Pada kelompok pertama, ayam divaksinasi dengan vaksin komersial AI (H5N1). Pada kelompok kedua, ayam tidak divaksinasi. Sampel darah dari kedua kelompok ayam dikoleksi dan dievaluasi titer antibodinya dengan teknik Hemaglutination Inhibition (HI). Hasil penelitian ini menunjukkan bahwa vaksin komersial AI (H5N1) bersifat imunogen yang baik karena dapat memicu pembentukan respon humoral protektif ayam petelur yang ditandai dengan peningkatan titer antibodi serum ayam yang divaksin.
THE DEVELOPMENT OF ASCARIDIA GALLI INFECTIVE EGGS BY IN VITRO CULTURE Balqis, Ummu; D, Darmawi; Hambal, Muhammad; Tiuria, Risa
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i2.3104

Abstract

The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%).Keywords: Ascaridia galli,  embrionated eggs
L3 POPULATIONS IN LAYING HENS INFECTED WITH 6,000 L2 OF ASCARIDIA GALLI D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedono, Retno D.; Pasaribu, Fachriyan H.
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3122

Abstract

The aim of the present study was to determine the survival of L3 populations in intestine ofchickens exposed to experimental Ascaridia galli infection. Nature female adult worm were obtained fromlumen of village chickens in a comercial abattoir in Bogor. The eggs (L1) obtained from uteri female adultworms were incubated in sterile aquadestilata at room temperature for 10-20 days developed embrionatedeggs (L2). Five groups (A-D) of 80 head chickens were infected with, 6000 L2 A. galli respectively. Thechickens of group A were infected six times with dose of each 1,000 L2 with an interval of one hour. Thechickens of group B were infected three times with dose of each 2,000 L2 with an interval of two hours.The chickens of group C were infected six times with dose of each 3,000 L2 with an interval of three hours. The chickens of group D were infected one time with single dose 6,000 L2. A. galli L3 were recovered from intestines of 80 heads chickens seven days after oesophagus inoculation with 6,000 L2.The result showed that total 702,000 L1 and 628,000 L2 collected from 124 A. galli female adult worms.The percentage of L1 developed L2 is 89.46% and L2 developed L3 is 11.27%. Significant survival of L3higher populations in intestine of chickens observed only in the group D. The results indicated thatchickens infected high dose of A. galli caused the decrease of host defence against ascaridiosis. Keywords: Ascaridia galli, embrionated eggs, larvae
RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF ASCARIDIA GALLI D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedoeno, Retno Damayanti; Pasaribu, Fachriyan Hasymi; Hambal, Muhammad; Daud, Razali
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.929

Abstract

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P 0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.
KONSENTRASI PROTEIN DAN PENENTUAN BERAT MOLEKUL EKSKRETORI/SEKRETORI L3 ASCARIDIA GALLI D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedono, Retno D; Pasaribu, Fachriyan H
Jurnal Kedokteran Hewan Vol 3, No 1 (2009): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i1.3074

Abstract

Penelitian ini bertujuan menentukan konsentrasi dan berat molekul protein  ekskretori/sekretori larva (L3) Ascaridia galli (A. galli). Larva L3 diperoleh dari usus halus 100  ayam tujuh hari setelah pemberian dosis 6000 L2 melalui esofagus ayam. Sebanyak 5?10  L3 dikultur secara in vitro  dalam setiap ml medium Rosswell Park Memorial Institute (RPMI 1640), pH 6,8, tanpa merah fenol dalam inkubator pada temperatur 37 0C dan 5% CO2 selama 3 hari. Ke dalam medium ditambahkan 100 unit ml-1 penisilin G, 100 µg ml-1 streptomisin, 5 µg ml-1 gentamisin dan 0,25 µg ml-1 kanamisin. Ekskretori/sekretori dipreparasi dari produk metabolisme L3 yang dilepaskan ke dalam medium kultur. Untuk mendapatkan protein ekskretori/sekretori, medium kultur dipekatkan dengan vivaspin 30.000 MWCO, dan kuantitas protein dihitung dengan metode Bradford. Berat molekul protein ekskretori/sekretori divisualisasikan dengan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Hasil penelitian  menunjukkan bahwa konsentrasi protein ekskretori/sekretori adalah 0,595 mg/ml dengan berat molekul 28 kDa.