Articles

Found 15 Documents
Search

THE CONTENT OF FATTY ACIDS IN INDONESIA’S FISH OIL Maulana, Indra T; Sukraso, Sukraso; Damayanti, Sophi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v6i1.8635

Abstract

Study of fatty acid content was conducted in six Indonesian fish oils taken from Surabaya, Sorong, Garut, Banyuwangi, and  fish oil standard. The acid and peroxide values were a main parameters which affecting the quality of the fish oil. These parameters were set using the method stated on the SNI No. 01-3555-1998. The oil containing omega-3 such as EPA and DHA is beneficial for health. The study of fatty acid content in six fish oils were analyzed by GCMS Shimadzu QP 2010 ULTRA with FID Detector. RTX-5 were used as a column (diphenyl dimethyl polysiloxane  as a solid buffer, size length 30 m, diameter 0,25 mm, and He as a gas mobile phase).  The results showed that acid values for oil 1, 2, 3, 4, 5, and 6 were 0.55%, 0.50%, 0.48%, 0.55%, 0.48%, and 0.58%, respectively. While the peroxide values were 5.67, 4.72, 4.45,5.01, 4.85, and 5.27 meq/kg, respectively. GCMS analysis showed that fish oil 1, 2, 3, and 4  very dominant containing squalene of  29.45%, 32.34%, 21.07%, and 43.49%, respectively. While oil 6 contained EPA of 8.97% and DHA 6.56%, and that was the highest compared with other oils. However, oil 6 also contained a trans fatty acids i.e., elaidic acid of 26.8% and trans-13-docosanoic acid of 0.9%. For comparison, natural oil 5 was rich of linoleic acid  (39.58%).  The GCMS's analysis results proved that the oil 6 made from lemuru had a big potency to be developed for an Indonesian fish oil export comodity. Keywords: fish oil, EPA, DHA, squalena, GCMS, acid value, peroxide value
SYNTHESIS AND CHARACTERIZATION MOLECULARLY IMPRINTED POLYMERS FOR ANALYSIS OF DIMETHYLAMYLAMINE USING ACRYLAMIDE AS MONOMER FUNCTIONAL Amin, Saeful; Damayanti, Sophi; Ibrahim, Slamet
Jurnal Kefarmasian Indonesia VOLUME 8, NOMOR 2, AGUSTUS 2018
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.25 KB) | DOI: 10.22435/jki.v8i2.330

Abstract

A selective separation techniques with Molecularly Imprinted Polymer (MIP) for High-Performance Liquid Chromatography (HPLC) has been developed for the assay of Dimethylamylamine (DMAA) doping compounds. Molecular imprinted polymer (MIP) is a technique to produce a polymer having the cavity due to the disposal of the templates, in which the cavity serves to recognize the molecules of the same size, structure, chemical and physical properties. The selectivity and affinity of the templates itself will increase, while the concentration value is increasing. MIP is made by DMAA as template, acrylamide as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross linking, azobisisobutyronitrile (AIBN) as the initiator and chloroform as a porogen solvent; using bulk method. The aim of research are conduct the MIP for the DMAA compound analysis, then the formed MIP is characterized by using Fourier Transform Infra Red (FTIR) and Scanning Electron Microscopy (SEM) to find out the polymer complexes formed and the morphological form of the MIP. The MIP formed then was analyzed by using High-Performance Liquid Chromatography (HPLC) to know the amount of the DMAA, the adsorption capacity, and the adsorption condition found in the MIP. The result of analysis on the content of DMAA in the MIP by using UV-Vis Spectrophotometer is 1.957 mg. Scanning Electron Microscopy (SEM) shows that the MIP has irregular and rough morphological structure; while the NIP has irregular morphology structures and smooth surfaces shape
SIMULTANEOUS DETERMINATION OF PARACETAMOL AND IBUPROFENE MIXTURES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Damayanti, Sophi; Ibrahim, Slamet; Firman, Kurnia; Tjahjono, Daryono H
Indonesian Journal of Chemistry Vol 3, No 1 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21899

Abstract

Analytical method for the determination of paracetamol and ibuprofene mixtures has been developed by High Performance Liquid Chromatography using C-18 column and acetinitrile - phosphate buffer pH = 4.5 (75:25) containing 0.075% sodium hexanesulfunate as a mobile phase. The detector was set at 215 nm. Using such conditions, retention time for paracetamol and ibuprofen was 4.89 and 7.11 min, respectively. The recovery for paracetamol and ibuprofen was found to be 101.07± 0.73% and 102.02 ± 1.58%, respectively. The detector limits of the method was 1.30 and 1.60 ?g/mL with the relative standard deviation (RSD) 0.74 and 1.52% for paracetamol and ibuprofen, respectively.
IN SILICO STUDY ON INTERACTION AND PRELIMINARY TOXICITY PREDICTION OF ELEUTHERINE AMERICANA COMPONENTS AS AN ANTIFUNGAL AND ANTITOXOPLASMOSIS CANDIDATE Damayanti, Sophi; Martak, Nadiyah Athifah Salim; Permana, Benny; Suwandi, Adi; Hartati, Rika; Wibowo, Indra
Indonesian Journal of Chemistry ARTICLE IN PRESS
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.48570

Abstract

Red bulbs of Eleutherine americana (Aubl.) Merr. ex K. Heyne has been known for its high content of naphthoquinones that have antifungal and antiparasitic activities. In this research, in silico interaction study was performed between 31 compounds reported to be found in E. americana with the selected target proteins for antifungal and antitoxoplasmosis activity using the molecular docking method. An ORPs (OSBP-related proteins), Osh4 (PDB ID: 1ZHX), and N-myristoyltransferase (Nmt, PDB ID: 1IYL) were used as the antifungal target proteins. Toxoplasma gondii purine nucleoside phosphorylase (TgPNP, PDB ID: 3MB8) and calcium-dependent protein kinase-1 (TgCDPK1, PDB ID: 4M84) were used as antitoxoplasmosis target proteins. Three-dimensional structures of the test compounds were made and optimized using GaussView 6.0 and Gaussian 09W. The target proteins were prepared using the Discovery Studio 2016 Program. Aquatic toxicity prediction as the preliminary assessment of the safety of the compounds was performed using ECOSAR v2.0. The results suggest that the compound having both the smallest free binding energy compared with positive control and other test compounds and low predicted toxicity is ?-sitosterol with a free binding energy of ?11.55 kcal/mol and ?11.18 kcal/mol towards Osh4 and Nmt and ?8.06 kcal/mol and ?10.29 kcal/mol towards TgPNP and TgCDPK1, respectively.
StudiIn SilicoSenyawaTurunan8-Tersubtitusi-7-Methoxy-2h-Chromen-2-OneSebagaiPenghambatEnzim Telomerase Tahir, Masdiana; Damayanti, Sophi; Hadi, Daryono; Tjahyono, Tjahyono
Jurnal Farmasi Indonesia Vol 10, No 2 (2018)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v10i2.588

Abstract

Adjustment of telomere length by telomerase is considered as a biological marker which determine the proliferation of cancer cells. The telomerase activity in cancer cells is potential to be targeted for cancer therapies. The aim of this study was QSAR studies to determine the descriptors that affect the anticancer activity of 8-substituted-7-methoxy-2H-chromen-2-one derivatives. The descriptors were calculated by using software MOE® 2009.10. to design new derivative compounds that have anticancer potency, to predict pharmacokinetic properties by using preADMET, and to predict tocixity by ADMET predictorTM and interaction predict to telomerase by using Autodock 4.2.6  The results showed QSAR equation was log IC50 = -1.358 (± 1.149) - 2.957 (± 1.019) × AM1_LUMO + 0.041 (± 0.011) × E_vdw + 2.367 (± 0.748) × glob + 0.487 (± 0.100) × log S. Fourteen of the new derivative compounds were designed based on QSAR equation revealed higher activity prediction than the lead compound and performed good absorption in the intestine. Compound (33) is predicted to have low permeability in Caco-2 cells and weakly bound to plasma proteins. Compound (50) predicted weakly bound to plasma proteins as well. Toxicity prediction showed that 9 derivatives compounds were predicted to have lower nearly equal toxicity to the toxicity of the lead compound. The study of the interaction of compounds with the receptor showed that derivatives (2), (4), (40) and (41) have the highest affinity to the receptor TERT with the binding free energy is −9.60 kcal / mol, −9.39 kcal / mol, −9.20 kcal / mol and -9.08 kcal / mol. Based on QSAR study, pharmacokinetic profile, toxicity, and the study of the interaction, four compounds have the potential to be develop as anticancer with telomerase inhibition.
Lactobacillus plantarum as Biopreservative Agent in Paneer for Dietary Food of Diabetic and Coronary Heart Disease Patients Singgih, Marlia; Ahsogan, Kanaka L.; Puspitasari, Irma M.; Damayanti, Sophi
Indonesian Journal of Clinical Pharmacy Vol 6, No 1 (2017)
Publisher : Indonesian Journal of Clinical Pharmacy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.595 KB) | DOI: 10.15416/ijcp.2017.6.1.38

Abstract

Paneer is a food product that is obtained by heating milk followed by acid coagulation. It is known asa diet food that is recommended for diabetic and coronary heart disease patients. A relatively shortershelf life of paneer is considered to be a major hurdle in its production. The present preliminary studywas conducted to determine antibacterial activity of Lactobacillus plantarum against Eschericia coliand Bacillus cereus, to develop it as biopreservative agent in paneer before using it for dietary food ofdiabetic and coronary heart disease patients in next clinical study. The antibacterial activities were testedthrough Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)using microdilution well method. Biopreservative activity in paneer was tested using total plate countmethod based on time and temperature variations. Result showed that Lactobacillus plantarum inhibitedBacillus cereus and Eschericia coli with MIC of 3125 and 1562.5 μg/mL whereas MBC was in a value of>6250 and >3125 μg/mL, respectively. As a biopreservative agent, addition of Lactobacillus plantarumto paneer showed no bacterial growth until 7 days in room temperature and 9th day in cold temperature. Itis concluded that Lactobacillus plantarum could be used as a natural biopreservative agent for extendingthe shelf life of paneer. The paneer with addition of Lactobacillus plantarum as biopreservative will thenbe consumed by diabetic patient and coronary heart patients in next clinical study.
Molecular Docking, Dynamics Simulation, and Scanning Electron Microscopy (SEM) Examination of Clinically Isolated Mycobacterium tuberculosis by Ursolic Acid: A Pentacyclic Triterpenes Pitaloka, Dian Ayu Eka; Damayanti, Sophi; Artarini, Aluicia Anita; Sukandar, Elin Yulinah
Indonesian Journal of Chemistry Article in press
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.332 KB) | DOI: 10.22146/ijc.33731

Abstract

The purpose of this study was to analyze the inhibitory action of ursolic acid (UA) as an antitubercular agent by computational docking studies and molecular dynamics simulations. The effect of UA on the cell wall of Mycobacterium tuberculosis (MTB) was evaluated by using Scanning Electron Microscopy (SEM). UA was used as a ligand for molecular interaction and investigate its binding activities to a group of proteins involved in the growth of MTB and the biosynthesis of the cell wall. Computational docking analysis was performed by using autodock 4.2.6 based on scoring functions. UA binding was confirmed by 30 ns molecular dynamics simulation using gromacs 5.1.1. H37Rv sensitive strain and isoniazid-resistant strain were used in the SEM study. UA showed to have the optimum binding affinity to inhA (Two-trans-enoyl-ACP reductase enzyme involved in elongation of fatty acid) with the binding energy of -9.2 kcal/mol. The dynamic simulation showed that the UA-inhA complex relatively stable and found to establish hydrogen bond with Thr196 and Ile194. SEM analysis confirms that UA treatment in both sensitive strain and resistant strain affected the morphology cell wall of MTB. This result indicated that UA could be one of the potential ligands for the development of new antituberculosis drugs.
ISOLATE OF HETEROTROPHIC MICROALGAE AS A POTENTIAL SOURCE FOR DOCOHEXAENOIC ACID (DHA) Julianti, Elin; Fathurohman, Mochamad; Damayanti, Sophi; Kartasasmita, Rahmana Emran
Marine Research in Indonesia Vol 43, No 2 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2269.65 KB) | DOI: 10.14203/mri.v43i2.264

Abstract

Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA were conducted by using gas chromatography. The results were four isolates of heterotropic microalgae, namely MTKC1, MTKC2, MTKC3, and MTKC4. The extract of MTKC2 that only showed the content of DHA with value of 9.2 % w/w. Therefore MTKC2 is a potential source for DHA. The MTKC2 was further identified by using molecular biology method and confirmed as Thraustochytrium aureum.
EVALUASI FORMULA KRIM MINYAK BIJI DELIMA (PUNICA GRANATUM L.) DAN UJI AKTIVITAS ANTIOKSIDAN DENGAN METODE β-CAROTENE BLEACHING Mita, Nur; Tarini D., Sasanti; Damayanti, Sophi
Journal of Tropical Pharmacy and Chemistry Vol 3 No 2 (2015): Journal of Tropical Pharmacy and Chemistry
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.73 KB) | DOI: 10.25026/jtpc.v3i2.97

Abstract

Minyak biji delima memiliki aktivitas antioksidan kuat, sehingga berpotensi untuk diformulasi menjadi sediaan antioksidan topikal. Tujuan penelitian ini adalah untuk mengevaluasi formula sediaan krim minyak biji delima dan menguji aktivitas antioksidan dari sediaan tersebut. Krim a/m diformulasi menggunakan emulgator Tween 80- Span 80. Evaluasi sediaan meliputi pemeriksaan pH dan viskositas sediaan yang disimpan selama 4 minggu pada suhu kamar, stabilitas fisik dengan metode Freeze Thaw sebanyak 4 siklus dimana 1 siklus terdiri dari 48 jam pada suhu 4°C dan 48 jam pada suhu 40°C. Potensi antioksidan diuji dengan metode β-Carotene Bleaching. Formula krim yang mengandung minyak biji delima 1% stabil secara fisik. Hasil uji efek antioksidan menunjukkan persen penghambatan minyak biji delima murni dan minyak biji delima dalam bentuk krim terhadap penurunan warna β-Carotene berturut-turut adalah 73,41% dan 52,80%. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa aktivitas antioksidan minyak biji delima dalam bentuk krim mengalami penurunan 0,3 kali.Kata kunci: krim, minyak biji delima, antioksidan, β-Carotene BleachingABSTRACTPomegranate seed oil has a potent antioxidant activity that is potential to be formulate into topical antioxidant dosage form. The purpose of this study is to formulate cream of pomegranate seed oil that physically stable, however have antioxidant activity. Cream w/o was formulated using Tween 80 - Span 80 as emulsifier. Evaluation of cream products includes determining pH and viscosity of the preparations stored for 4 weeks at room temperature, physical stability test by Freeze Thaw method which was carried out for 4 cycles in where 1 cycle consists of 48 hours at 4°C and 48 hours at 40°C. Antioxidant activity was tested by β-carotene bleaching method. The results of the research showed that all cream formulas containing pomegranate seed oil 1% were physically stable and can increase the comfortness in use on the skin. The antioxidant test results showed antioxidant activity of pure pomegranate seed oil and pomegranate seed oil as formulated in creams have antioxidant capacity with inhibition percentage of 73.41% and 52.80%, respectively. Based on these results it can be concluded that the antioxidant activity of pomegranate seed oil in cream decreased 0,3 times.Keywords: cream, pomegranate seed oil, antioxidant, β-Carotene Bleaching
ISOLATE OF HETEROTROPHIC MICROALGAE AS A POTENTIAL SOURCE FOR DOCOHEXAENOIC ACID (DHA) Julianti, Elin; Fathurohman, Mochamad; Damayanti, Sophi; Kartasasmita, Rahmana Emran
Marine Research in Indonesia Vol 43 No 2 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2269.65 KB) | DOI: 10.14203/mri.v43i2.264

Abstract

Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA were conducted by using gas chromatography. The results were four isolates of heterotropic microalgae, namely MTKC1, MTKC2, MTKC3, and MTKC4. The extract of MTKC2 that only showed the content of DHA with value of 9.2 % w/w. Therefore MTKC2 is a potential source for DHA. The MTKC2 was further identified by using molecular biology method and confirmed as Thraustochytrium aureum.