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EFEK IMUNOMODULATOR EKSTRAK AIR CINCAU HITAM (MESONA PALUSTRIS BL) TERHADAP KARSINOGENESIS MENCIT [IMMUNOMODULATORY EFFECTS OF WATER EXTRACTS OF BLACK CINCAU (MESONA PALUSTRIS BL) ON CARCINOGENESIS IN MICE] W., Tri Dewanti; Sukardiman, .; P., Djoko Agus; Darmanto, Win
Jurnal Teknologi dan Industri Pangan Vol. 23 No. 1 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Janggelan or black cincau (Mesona palustris BL) is commonly consumed as dessert and also used as herbal remedy in the folk medicine. This study aimed to test the potency of water extract of black cincau in inhibiting fibrosarcoma cancer induced by benzo(a)pyrene. Mice used in this study were divided into 6 groups. Solution of benzo(a)pyrene 0.3% was given during two days for five times to induce cancer. Water extract of black cincau at concentration of 100, 500 and 1000 mg/kg BW was given daily for 2 weeks before initiation (induction of benzo(a)pyrene) during the initiation and until 4 weeks after initiation. The IFN-? was observed with IFN-y ELISA Kit immunosurveillance parameters while the CD8+, NK cells and macrophages were observed with a flow cytometer. Cancer incidence was observed by palpation every week until 10 weeks after benzo(a)pyrene last induction. The results showed that a high dose of water extract of black cincau (1000 mg/kg BW) increased IFN-? expression and immunosurveillance of CD8+, NK cells and macrophages of the mice. The treatment also increased cell apoptosis and reduced the cancer incidence in mice by 57%.
THE EFFECTIVENESS OF HERBAL MOSQUITO COILS “MORIZENA” AGAINST Aedes Aegypti DEATH Susilowati, Rina Priastini; Darmanto, Win; Aminah, Nanik Siti
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 2 (2018)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (625.03 KB) | DOI: 10.20473/ijtid.v7i2.7324

Abstract

It has been successfully found new herbal mosquito coils called “Morizena” made from Passiflora foetida leaf extract (40%), Chrysantemum cinerariaefolium flowers seed extract (40%), essential oils of  leaves-stems of Cymbopogon nardus (20%). The herbal mosquito coils are more effective to kill Aedes aegypti compared to commercial synthetic mosquito coils made from Transfluthrin. Treatment of exposure to herbal mosquito coils “Morizena” on Aedes aegypti for 8 hours/day with concentration 500 ppm (P1), 1000 ppm (P2), 2000 ppm (P3), 3000 ppm (P4), 4000 ppm (P5) and treatment of exposure to synthetic mosquito coils Transfluthrin 2500 ppm (K1) as a positive control, and without treatment of exposure to mosquito coils (K0) as a negative control. Experimental animals used were adult Ae. aegypti mosquito with 25 mosquitoes in each treatment. Experimental design used is one-way Anova test with linear regression to calculate its LC50 and LC90. The results of test to herbal mosquito coils “Morizena” given for 8 hours/day obtained mortality of Ae. aegypti by 92% to a concentration of 3000 ppm (P4) and 100% to a concentration of 4000 ppm (P5) and 100% for test to synthetic mosquito coils transfluthrin 2500 ppm (K1). Ae. aegypti LC50 and LC90 value for treatment of exposure to herbal mosquito coils “Morizena” are 999 ppm and 2977 ppm. Treatment of herbal mosquito coils “Morizena” with graded doses up to 4000 ppm and synthetic mosquito coils Transfluthrin 2500 ppm causing an increase in the enzyme acetylcholinesterase activity of Ae. aegypti. The conclusion is based on the Ae. aegypti  LC90 value is 2977 ppm, which means the effective dose of herbal mosquito coils “Morizena” to kill Ae. aegypti is 2977 ppm.
Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa Wahyuningsih, Sri Puji Astuti; Savira, Nadyatul Ilma Indah; Darmanto, Win
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6957

Abstract

Pseudomonas aeruginosa is an opportunistic bacterium that causes infections in human. The wall cell of its bacteria contains lipopolysaccharide as virulency factors to protect it from human immunity. Lipopolysaccharide can inhibit phagocytosis in the body. Polysaccharide krestin (PSK) from Coriolus versicolor extract contains ?-glucan that can increase phagocytic activity and capacity. This research aimed to identify the effect on polysaccharide krestin from C. versicolor extract on phagocytic activity and capacity of mice exposed by P. aeruginosa. The design of this research was experimental design. There were six treatment groups. The phagocytic activity and capacity were counted on slide smears of mice peritoneal fluid. The data was analyzed by using one way ANOVA. The results of the phagocytic activity and capacity showed that PSK was added before exposure (P1) or after exposure (P2) or before-after exposure (P3) had potential to increase phagocytic activity and capacity. The conclusion of the research was that adding polysaccharide krestin either before exposure or after exposure or both of them could increase phagocytic activity and capacity. The benefits of this research to development of science are expected to reduce human infection and to utilize natural ingredients as immunomodulator.How to CiteWahyuningsih, S. P. A., Savira, N. I. I, & Darmanto, W. (2016). Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa. Biosaintifika: Journal of Biology & Biology Education, 8(3), 308-313.
Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Putri, Intan Permata; Savira, Nadyatul Ilma Indah; Winarni, Dwi; Suhargo, Listijani; Darmanto, Win
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11284

Abstract

Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p < 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p < 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.
ACUTE TOXICITY TEST OF POLYSACCHARIDES KRESTIN FROM CORIOLUS VERSICOLOR EXTRACT WITH PARAMETERS OF HEPATOCYTE DAMAGES, SGPT AND SGOT ENZYME IN MICE Mandasari, Andita Ayu; Wahyuningsih, Sri Puji Astuti; Darmanto, Win
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (654.598 KB) | DOI: 10.22146/jsv.8108

Abstract

Coriolus versicolor is a mushroom that has polysaccharopeptide krestin (PSK) and polysaccharopeptide (PSP). Many reports showed that polysaccharide krestin as a compound which could reduce mutagen induction, radiation, and development of cancer spontaneusly. However, all substances entering in body could change become toxic depending on dosage. Therefore, this research was aimed to know the effects of PSK on hepatocyte damages, SGPT and SGOT enzymes. Polysaccharide krestin was given by intraperitoneal injection once with treatment groups as follow: K0, was given only saline; P1, was given 80 mg/kg BB PSK dosage; P2, was given 120 mg/ kg BB PSK dosage; P3, was given 160 mg/ kg BB PSK dosage; P4, was given 200 mg/ kg BB PSK dosage; dan P5, was given 240 mg/ kg BB PSK dosage. The results showed that PSK caused hepatic lesions, such as parenchymatous (hydrophic) degeneration of hepatocytes in groups P2 and P3, mild to moderate necrosis in group P4, and moderate to severe necrosis in group P5. Level of SGPT enzyme was not increased, butlevel of SGOT enzyme was increased in mice given 160 mg/ kg BB dosage with value 151,62 ± 26,62 IU/ L.
Expression and purification of recombinant coat protein of sugarcane mosaic virus from Indonesian isolate as an antigen for antibody production Astuti, Natalia Tri; Darsono, Nurmalasari; Widyaningrum, Suvia; Sawitri, Widhi Dyah; Astuti, Sri Puji; Darmanto, Win
Indonesian Journal of Biotechnology Article in Press
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.263 KB) | DOI: 10.22146/ijbiotech.45551

Abstract

Sugarcane mosaic virus (SCMV, genus Potyvirus, familia Potyviridae) is a prominent pathogen of sugarcane (Saccharum sp. hybrids). It can cause losses in susceptible varieties, in crop as well as sugar production economically. Although it has been studied in major sugar production countries, researches on the definement of SCMV from Indonesian isolate based on molecular study are very limited. This study aimed to obtain a proper recombinant antigens emanating from coat protein of SCMV from Indonesian isolate for polyclonal antibody production that can be used for immunodiagnosis purposes. A gene encodes the coat protein of SCMV (CP-SCMV) was amplified by the reverse transcription polymerase chain reaction and cloned into vector pJET1.2. The cDNA was inserted into 6X His-tag expression plasmid of pET28a(+) and overexpressed in Escherichia coli BL21(DE3) to produce recombinant protein. The highest expression had performed on 0.1M IPTG induction media for 5 hours at 37oC. SDS-PAGE analysis clarified that recombinant CP-SCMV remains as an insoluble fraction. The purifications were performed by the affinity Ni-NTA resin which followed by electroelution to obtain highly purified proteins. To meet quality requirements of a proper antigen the highly purified protein was concentrated. Molecular weight of rCP-SCMV (approximately 40 kDa) was clearly observed by 10% SDS-PAGE at the concentration of 16.184 mg/mL. 
EFFECT OF SAPONIN-PODS EXTRACT ACACIA (ACACIA MANGIUM) TO HEMATOCRIT, HEMOGLOBIN AT TILAPIA (OREOCHROMIS NILOTICUS) Yuniar, Is; Darmanto, Win; Soegianto, Agoes
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Problem of aquaculture shrimp, one of which is wild fish, competitor. Usually to eliminate the use of saponin from tea seed. saponin to kill fish competitors, (tilapia) are entered when filling water. Saponin to kill fish competitors, (tilapia) are entered when filling water. Saponin used from tea seed meal is imported. Indonesia is rich in biological resources of plants / substances that secondary metabolites naturally untapped optimal. The study was conducted in a laboratory scale with a completely randomized factorial design, using a series of six concentrations and three replicates for each species. LC50 analysis using probit analysis. Unfortunately, there was no scientific information for it. It has been done a laboratory work to study chemical compounds of pod Acacia (Acacia mangium) by phytochemical screening and measure those quantitatively. The result of these were that pod Acacia (Acacia mangium) contain alkaloid, flavonoid, tanin, saponin, steroid, and terpenoid. The results of this study indicate LC50-96h values for Pod Acacia mangium at salinity 0, 5, 10 and 15 promil is 5.487 ppm, 4.313 ppm, 3.985 ppm and 2.944. Value hemoglobin at 0promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 11.353±0.63)g/dL,10.607±0.18g/dL, 9.860±0.36 g/dL and 8.80±0.60. Value hemoglobin at 5promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 8.86±1.02 g/dL,7.76±0.943 g/dL, 7.42±0.71 g/dL and 4.68±0.53g/dL. Value hemoglobin at 10 promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 7.34±1.95 g/dL,6.07±0.755 g/dL, 5.23±1.34 g/dL and 3.23±035 g/dL. Value hemoglobin at 15promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 8.570±0.478 g/dL,5.18±0.876 g/dL, 3.42±0386 g/dL and 3.817±0.355g/dL. Value hematocrit at 0promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 35.033±3.32g/dL, 31.2±0.90 g/dL, 29.367±0.97 g/dL and26.60±1.345. Value hematocrit at 5promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 25.633±1.24 g/dL, 23.90±2.68 g/dL, 18.367±2.554 g/dL and 12.867±2.38 g/dL. Value hematocrit at 10 promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 21.567±6.134 g/dL,18.567±1.48 g/dL,14.767±3.46 g/dL and 10.53±3.38 g/dL. Value hematocrit at 15promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 26.100±1.42 g/dL, 15.667±2.79 g/dL,9.933± 2.04 g/dL and 11.933±1.19 g/dL. Saponin of acacia may lower hematocrit and hemoglobin an indication of damage to the red blood cells occurs hemolysis.