Latifah K. Darusman
Departemen Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Jl. Meranti, Kampus IPB Dramaga, Bogor, 16680, Indonesia Pusat Studi Biofarmaka, Institut Pertanian Bogor, Jl. Taman Kencana No. 3, Bogor, Indonesia

Published : 22 Documents
Articles

Found 22 Documents
Search

KEANEKARAGAMAN JENIS TUMBUHAN OBAT DARI HUTAN KERANGAS Kissinger, Mr.; Zuhud, Ervizal AM.; Darusman, Latifah K.; Siregar, Iskandar Z.
Jurnal Hutan Tropis Vol 1, No 1 (2013)
Publisher : Jurnal Hutan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In this study, identification of medicinal plant from kerangas forest was performed by semi structural interview. Villagers who lived around kerangas forest were interviewed for etnobotanical knowledges. This study was conducted in one main locations of kerangas forest, namely Guntung Ujung District Banjar South Kalimantan. Three locations were selected as reference locations of study:i) Kotawaringin Timur Central Kalimantan ii) Nyaru Menteng Central Kalimantan iii) Tanjung-Muara Kelanis South Kalimantan- Central Kalimantan. Results showed that kerangas forest contained 36 species of plants which used for medicine. Some potential biocativities could be improved based on traditional knowledge for antibacterial, antioxidant, antiplasmodium, antihipertensi, and antidiabetes Beside that, there were another beneficial using from species plants of kerangas forest. Villagers have used various species plants of kerangas forest for furniture, food, spiritual using, ornamental crops, dye, handicraft, and fire wood. It can be concluded that plants diversity of kerangas forest had potential functions as source of beneficial using plant products.
SUPLEMENTASI STEROL LEMBAGA GANDUM (TRITICUM SP.) PADA MARGARIN (SUPPLEMENTATION OF MARGARINE WITH WHEAT GERM STEROL) Marliyati, Sri Anna; Syarief, Hidayat; Muchtadi, Deddy; Darusman, Latifah K.; ., Rimbawan
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.419 KB)

Abstract

Margarine is a water in oil (w/o) emulsion product which is widely used for household cooking and baking industry. Consuming of margarine, which contains trans fatty acid may cause health problem due to the increase of LDL cholesterol. Since margarine is also a good carrier of phytosterol which prevent the absorption of cholesterol, there is a possibility to formulate a healthier margarine. In this research formulation and characteristics of products was investigated. The research work consisted of two steps: (1) supplementation of wheat germ sterol into margarine (two methods) and  (2) analysis of physical, chemical characteristics and hedonic score. Parameters of physical characteristics were melting point and emulsion stability, whereas chemical characteristics were water and oil contents. The hedonic test was carried out based on product?s color, odor, taste, texture, and spreadability. Results showed that method II of supplementation produced better margarine than method I, in which the concentration of sterol in the margarine was higher with a melting point similar to that of control, better emulsion stability, and higher hedonic score. Supplementation process was carried out by mixing sterol into fat phase melted at 50 0C, followed by mixing with aqueous phase at 4 0C. Sterol used for method II was extracted using mixed solvent of hexane and ethanol at the ratio of 1:2 (v/v), which was resulted from previous experimentation.
BIOLOGICAL ACTIVITIES AFFORDED BY THE EXTRACT FROM RARU BARK TO INHIBIT ACTION OF ALPHA- GLUCOSIDASE ENZYMES Pasaribu, Gunawan; Syafii, Wasrin; Darusman, Latifah K.
Indonesian Journal of Forestry Research Vol 8, No 1 (2011): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Raru (Shoreabalanocarpoides Sym) signifies one of the tree species that grows widespread in Sumatra Island.  Its bark portion is commonly used by local villagers as additional ingredient mixed to nira (sugar palm juice).  This addition is intended to make the juice more durable and also to enrich its taste after the juice is previously fermented to become traditional toddy beverage or the so-call “tuak”.  Local villagers believe that raru bark can reduce the level of blood sugar. As the relevance, the research was conducted to confirm that the extract from raru bark could afford its biological activities to inhibit alpha-glucosidase enzyme through its characterization, quantification, and isolation of its boactive compound. The extraction was performed using two methods (i.e.reflux and maceration techniques). Result revealed that the bark extract obtained from both techniques contained polyphenol compounds: flavonoid, saponin and tannin. Further, raru-bark extract from the reflux and maceration techniques could inhibit the action of alpha glucosidase enzymes on carbohydrate substrate ( i.e. p -nitrophenil-α-D-glucopyranose), at respectively 90.67% and 97.33%. Meanwhile, the inhibition activities afforded by the patented drug as a control (i.e. glucobay) equaled to 97.05%.  Assesment using UV-VIS spectroscopy, showed that the maximum spectrum of bioactive compound in the extract was at the wave length of 288.6 nm. Scrutiny using FTIR spectroscopy could identif y the presence of aromatic groups in the compound, containing -OH, C-H, C=C, C-O and C-H bond types. Analysis using GC-MS exhibited that the compound had molecular weight of 390 with molecular structure as C20H22O8. Ultimately, data analysis scrutiny with the aid of NMR judged the most plausible compound as bioactive was 4-Glucosyl-3, 4’, 5-trihydroxystilbene.
RESPON EKSPRESI MOLEKUL ADHESI PADA KULTUR SEL ENDOTEL YANG DIINDUKSI LDL TEROKSIDASI DARI MACACA FASCICULARIS TERHADAP EKSTRAK KURKUMINOID TEMUMANGGA Susmiati, Trini; ., Sulistiyani; Sajuthi, Dondin; Darusman, Latifah K.
Jurnal Primatologi Indonesia Vol. 6 No. 2 (2009)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.298 KB)

Abstract

Curcuminoids of Curcuma manggo believed to have antioxidant properties that can prevent the occurrence of atherosclerosis.  However, the mechanism at the molecular level is not clearly known. Increase in intracellular adhesion molecule-1 (ICAM-1) will result in endothelial cell dysfunction that initiates athersclerosis. The expression of ICAM-1 can be triggered by oxidized LDL molecules. The purpose of this study is to elucidate the effects of curcuminoids temumangga (Curcuma mango) on the expression of ICAM-1 induction in endothelial cell culture. Prior to the induction of the expression of ICAM-1, endothelial cell culture was pre-incubated with curcuminoids extract for 48 hours with concentration of 2 ppm and 8 ppm, then further incubated with oxidized LDL for 24 hours. The treated cells were compared to 5 µM Cu2+ induced endothelial cell culture with or without LDL  from Macaca fascicularis.  ICAM-1 expression was determined by immunohistochemistry using anti-ICAM-1 antibodies and visualized with staining by 3.3-diaminobenzidin tetrahidrochloride (DAB). Results showed that surface-induced endothelial cell culture with ion Cu2+ stained well by DAB as brown yellow, which indicates ICAM-1 expression. Curcuminoids extract of  8 ppm reduced induction of ICAM-1 response. Induction of ICAM-1 expression by oxidized LDL caused foam cell accumulation in addition to ICAM-1 expression.Key words: Curcuminoid, low density lipoprotein (LDL), Macaca fascicularis, intracellular adhesion molecule-1 (ICAM-1), immunohistochemistry
PENGATURAN PEMBUNGAAN TANAMAN MANGGIS (GARCINIA MANGOSTANA L.) DI LUAR MUSIM DENGAN STRANGULASI, SERTA APLIKASI PAKLOBUTRAZOL DAN ETEPON Rai, I Nyoman; Poerwanto, Roedy; Darusman, Latifah K.; Purwoko, Bambang S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 32 No. 2 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1787.566 KB) | DOI: 10.24831/jai.v32i2.1438

Abstract

The objective of the research was to study the application of strangulation, packlobutrazol and ethephon to stimulate flowering of mangosteen trees. The experiment was conducted from June 2002 to September2 003, located at Farmer's Mangosteen Orchards, Leuwiliang, Bogor. A randomized block design with two factors was used in thisexperiment. The first factor was treatments to stimulate flowering consisted of three levels i.e strangulation (8), (S) application of paclobubtrazol (P) and control (K). The second factor was treatments for dormancy breaking consisted of two levels i.e without ethephon (Eo) and with ethephon (EiJ. Each treatment was replicated four times. The resultshowed that strangulation and application of paclobutrazol were effective in stimulating flowering of mangosteen trees. Those treatments decreased gibberellic acid content and increased total sugar and C: N ratio. Interaction effect was found between treatments to stimulate flowering and ethephon on number of flowers, number of fruits and weight of fruits per tree. The highest fruits weight (2.,50 kg/tree) was obtained on the combination of paclobutrazol with ethephon while the lowest was in control without ethephon( 6.05 kg/tree).Key words: Mangosteen, Strangulation, Paclobutrazol, Ethephon, Flowering;
A LABORATORY SCALE RESEARCH TO OBTAIN ENVIRONMENTALLY SAFE EFFLUENT FROM FINAL WASTE DISPOSAL SITE LEACHATE IN GALUGA OWNED BY REGIONAL GOVERNMENT OF BOGOR CITY WAS CONDUCTED FROM JULY 2006 THROUGH TO APRIL 2007. THE EXPERIMENT OF PRODUCTION TOLERABLE THRESHOLD EFFLUENT WAS CARRIED OUT BY AERATING THE LEACHATE IN 4 DIFFERENT AERATION RATES (0, 10, 30 AND 70 LITERS/MINUTE).  THE RESEARCH FOUND THAT THE MOST EFFECTIVE METHOD TO REDUCE POLLUTANT WAS AERATING AT THE RATE OF 70 LITERS/MINUTES.  HOW ., Nurhasanah; Darusman, Latifah K.; Sutjahjo, Surjono Hadi; Lay, Bibiana Widiati
Forum Pasca Sarjana Vol. 34 No. 1 (2011): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A laboratory scale research to obtain environmentally safe effluent from Final Waste Disposal Site leachate in Galuga owned by Regional Government of Bogor City was conducted from July 2006 through to April 2007. The experiment of production tolerable threshold effluent was carried out by aerating the leachate in 4 different aeration rates (0, 10, 30 and 70 liters/minute).  The research found that the most effective method to reduce pollutant was aerating at the rate of 70 liters/minutes.  However, only some of pollutant (Cu, Zn, Pb and E.coli) can be reduced until below tolerable threshold.   Keywords: effluent, leachate, tolerable threshold
BIOLOGICAL ACTIVITIES AFFORDED BY THE EXTRACT FROM RARU BARK TO INHIBIT ACTION OF ALPHA- GLUCOSIDASE ENZYMES Pasaribu, Gunawan; Syafii, Wasrin; Darusman, Latifah K.
Indonesian Journal of Forestry Research Vol 8, No 1 (2011): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2011.8.1.32-49

Abstract

Raru (Shoreabalanocarpoides Sym) signifies one of the tree species that grows widespread in Sumatra Island.  Its bark portion is commonly used by local villagers as additional ingredient mixed to nira (sugar palm juice).  This addition is intended to make the juice more durable and also to enrich its taste after the juice is previously fermented to become traditional toddy beverage or the so-call “tuak”.  Local villagers believe that raru bark can reduce the level of blood sugar. As the relevance, the research was conducted to confirm that the extract from raru bark could afford its biological activities to inhibit alpha-glucosidase enzyme through its characterization, quantification, and isolation of its boactive compound. The extraction was performed using two methods (i.e.reflux and maceration techniques). Result revealed that the bark extract obtained from both techniques contained polyphenol compounds: flavonoid, saponin and tannin. Further, raru-bark extract from the reflux and maceration techniques could inhibit the action of alpha glucosidase enzymes on carbohydrate substrate ( i.e. p -nitrophenil-α-D-glucopyranose), at respectively 90.67% and 97.33%. Meanwhile, the inhibition activities afforded by the patented drug as a control (i.e. glucobay) equaled to 97.05%.  Assesment using UV-VIS spectroscopy, showed that the maximum spectrum of bioactive compound in the extract was at the wave length of 288.6 nm. Scrutiny using FTIR spectroscopy could identif y the presence of aromatic groups in the compound, containing -OH, C-H, C=C, C-O and C-H bond types. Analysis using GC-MS exhibited that the compound had molecular weight of 390 with molecular structure as C20H22O8. Ultimately, data analysis scrutiny with the aid of NMR judged the most plausible compound as bioactive was 4-Glucosyl-3, 4’, 5-trihydroxystilbene.
KARAKTERISTIK HABITAT MIKRO TABAT BARITO (Ficus deltoidea Jack) PADA TUMBUHAN INANGNYA Agustina, Anisa; Zuhud, Ervizal A.M.; Darusman, Latifah K.
Jurnal Penelitian Hutan dan Konservasi Alam Vol 12, No 1 (2015): Jurnal Penelitian Hutan dan Konservasi Alam
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tabat barito (Ficus deltoidea Jack.) merupakan tumbuhan yang berpotensi sebagai obat dan memiliki sebaran luas  di Indonesia,  meliputi  Jawa  Barat,  Jawa  Tengah,  Sumatera,  Kalimantan  dan  Sulawesi.  Di  Taman Nasional Gunung Gede Pangrango (TNGGP), tabat barito tumbuh sebagai epifit yang berpotensi sebagai sumber plasma nutfah bagi tujuan pengembangan budidaya. Tujuan dari penelitian ini adalah untuk memperoleh informasi tentang karakteristik habitat mikro tabat barito di Resort Mandalawangi TNGGP. Hasil penelitian  menunjukkan  bahwa  sebaran tabat  barito  hingga elevasi 1.800  m dpl.  Penelitian yang dilakukan memperoleh hasil, yaitu 178 individu tabat barito yang berasal dari 100 individu tumbuhan inang. Tabat barito terletak pada kisaran suhu 18,3°C-23,1°C, kelembaban udara relatif 80-84% dan kelerengan 4%-24% dengan arah lereng bervariasi. Tumbuhan inang memiliki karakteristik fisik yang bervariasi dalam hal tinggi pohon, diameter batang maupun luas tajuknya, namun memiliki persamaan dalam hal karakteristik kulit  batangnya,  yaitu  memiliki  permukaan  yang  kasar  dengan  kulit  batang  yang  mengelupas  maupun beralur.  Media  tumbuh  tabat  barito  pada  tumbuhan  inang  bervariasi,  mulai  dari  akar  yang  menempel langsung pada kulit batang hingga media tumbuh berupa hasil dekompisisi
STUDIES ON DORMANCY PERIODS AND GROWTH RHYTHM OF SHOOT AND ROOT OF MANGOSTEEN (GARCINIA MANGOSTANA L.) Hidayat, Ramdan; Surkati, Achmad; Poerwanto, Roedhy; Darusman, Latifah K.; Purwoko, Bambang S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 2 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.163 KB) | DOI: 10.24831/jai.v33i2.1516

Abstract

Mangosteen has a good prospectus for international market. Many people in the world like this fruit because it is delicious, has high nutrient contents, and it can be consumed as a fresh fruit. The growth of mangosteen is very slow with a very long juvenile period because of its long dormancy period. The objective of this research was to study the dormancy periods and growth rhytm of shoot and root of young and adult mangosteen seedlings. The research used completely randomized design and consisted of two experiments. The first experiment was to study the dormancy and flushing periods. The second experiment was to study the growth patterns of shoot and root foom several ages of mangosteen seedlings. This research was conducted at Mekarsari Fruit Park, Cileungsi, Bogor. The result of the experiment showed that two years old of mangosteen seedling had 5 flushes per year, four years old of mangosteen had 3-4 flushes per year, and eight years old of mangosteen only had 2 flushes. Dormancy periods were significantly different between 2,4 and 8 year of mangosteen seedling. After the seedlings have branches, the dormancy periods become twice as many as that before branching. The roots grew rapidly two weeks before flush and slower after flush occurred.   Key words: Garcinia mangostana, dormancy, flush, seedling
SINTESIS ADSORBEN ZEOLIT@AuNPs@MET MENGGUNAKAN EKSTRAK DAUN BINAHONG (Anredera cordifolia) SEBAGAI BIOREDUKTOR PREKURSOR Au DAN KARAKTERISASINYA (Synthesis Zeolite@AuNPs@MET Adsorbent by Binahong (Anredera cordifolia) Leaf Extract as Au Precursor Bioreduc Nurdiani, Nurdiani; Darusman, Latifah K.; Rohaeti, Eti
Jurnal Hasil Penelitian Industri Vol 28, No 1 (2015)
Publisher : Baristand Industri Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.353 KB)

Abstract

Modifying zeolite with Au nanoparticles and mercaptoethanol ligand(zeolite@AuNPs@MET) has been developed as an adsorbent of heavy metal ions. The preparation ofzeolite@AuNPs@MET composite material was done in three steps. Firstly, incorporating gold precursorin to zeolite cavity. Secondly, reduction of gold nanoparticles by binahong leaf extract. Thirdly, modifyingzeolite@AuNPs with mercaptoethanol ligand. Binahong was used as bioreductor agent in the synthesis ofAu nanoparticles since it has large contents of flavonoids, saponins, tannins, and steroids which containreducing functional group. The zeolite@AuNPs@MET composite material was characterized by XRD,EDX, PSA, TEM, FTIR and UV Visible Spectrophotometer. EDX measurements showed Au content of0.88%, TEM and PSA measurement showed Au nanoparticle size in the range of 7.12 nm to 14.45 nmwith an average size distribution of 110.6 nm while gold nanoparticles immobilized in the pores ofzeolites have sizes ranging from 4.98 nm to 9.50 nm with an average size distribution of 279 nm. UVVisible absorption spectrum revealed a new formed peak at 537 nm, indicating formation of AuNPs. The characteristic peaks (at 526 nm and 532 nm) were also found in the UV-Vis absorption spectrum ofAuNPs immobilized in the zeolite pores and AuNPs in the zeolite@AuNPs@MET composite, respectively.FTIR measurements of mercaptoethanol ligand showed the presence of a peak at 2550 cm-1 regionindicating SH functional groups, which disappeared after modification of zeolite@Au with MET ligand,indicated the –SH bond was broken and the remained –S was attached to AuNPs. All of thecharacterization revealed the success of the composite material preparation, which is an interestingmaterial expected to have highly potential as a heavy metal ion adsorbent.