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Aktivitas fosfatase dan produksi asam organik di rhizosfer dan hifosfer bibit kelapa sawit bermikoriza *) Phosphatase activity and organic acid production in rhizosphere and hyphosphere of mycorrhizal oil palm seedling WIDIASTUTI, Happy; SUKARNO, Nampiah; DARUSMAN, Latifah Kosim; GOENADI, Didiek Hadjar; SMITH, Sally; GUHARDJA, Edi
E-Journal Menara Perkebunan Vol 71, No 2: Desember 2003
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.025 KB) | DOI: 10.22302/iribb.jur.mp.v71i2.164

Abstract

SummaryStudies on the mechanism of the higher Puptake of oil palm seedling colonized witharbuscular mycorrhizal fungi throughsolubilizing of fixed P by organic acid orhydrolysis of organic P by phosphatase activityhave not been reported yet. This experiment wasaimed to examine the phosphatase activity andproduction of organic acids in rhizosphere andhyphosphere, mycorrhizal and non-mycorrhizaloil palm seedling. Oil palm seedling were grownfor 26 weeks in sterilized Cikopomayak acid soilin 20.5 cm diameter pots with three compart-ments, a central one for root growth(rhizosphere) and two adjacent on both side nextto the root compartment for hyphal growth(hyphosphere). Compartmentation was accom-plished by a 0.25 mm stainless steel filter. Allcompartment received a uniform concentration ofphosphorus (300 P mg kg -1 soil) either in organic(Na-phytate) or inorganic NH 4 HPO 4 form.Acaulospora tuberculata inoculum was establish-ed in pot culture using Pueraria phaseoloides as ahost, while Gigaspora margarita was propagatedusing maize as a host. AM fungal inoculumapplied as mixed propagules in optimum dosage.The experiment was conducted to asses ninetreatments combination between AM inoculation(without, A. tuberculata, and G. margarita) andsources of P (without P, inorganic P NH 4 HPO 4 ,and organic P Na phytate). Factorial in completerandomized design with two factors and threereplications was used in this research. In thehyphal compartment acid phosphatase activitywas much higher than alkaline phosphataseactivity, while in the rhizosphere alkalinephosphatase activity was higher compared toacid phosphatase activity. Acid phosphataseactivity in rhizosphere of oil palm seedlingsinoculated with A. tuberculata was significantlyhigher compared to uninoculated seedlings.However, both acid phosphatase activity andalkaline phosphatase activity were slightlyenhanced by mycorrhizal inoculation. In contrast,organic acid production between inoculatedseedling and uninoculated seedling was notsignificantly different. It seems that AM fungalsymbiosis with oil palm enhance mineralizationof organic P in spite of solubilization ofinorganic P.RingkasanMekanisme peningkatan pertumbuhankelapa sawit bermikoriza khususnya yangdisebabkan aktivitas pelarutan P anorganik yangterfiksasi melalui pelarutan oleh asam organikatau hidrolisis P organik oleh aktivitas fosfataseelum dilaporkan. Percobaan ini bertujuanmenetapkan aktivitas fosfatase dan produksi asamorganik di rhizosfer dan hifosfer, bibit kelapasawit bermikoriza dan tidak bermikoriza. Kelapasawit ditumbuhkan selama 26 minggu pada tanahmasam Cikopomnayak steril pada pot ber-diameter 20,5 cm yang terbagi atas tiga daerah,ruang tengah untuk pertumbuhan akar (rhizosfer)dan dua daerah di sebelahnya untuk pertumbuhanhifa (hifosfer). Penyekatan pot menggunakanfilter stainless steel berukuran lubang 0,25 mm.Semua daerah dipupuk P pada konsentrasi300 P mg kg -1 tanah baik dalam bentuk organik(Na-phytate) maupun anorganik (NH 4 HPO 4 )Inokulum CMA merupakan hasil perbanyakandengan sistem kultur pot menggunakan inangPueraria phaseoloides untuk Acaulosporatuberculata sedangkan untuk Gigasporamargarita menggunakan inang jagung. InokulumCMA berupa propagul campuran pada dosisoptimum. Percobaan dilakukan untuk mengujisembilan perlakuan yang merupakan kombinasiantara inokulasi CMA (tanpa, A. tuberculata,dan G. margarita) dan sumber P (tanpa P,anorganik P NH 4 HPO 4 , dan organik P Naphytate). Rancangan percobaan ialah rancanganacak lengkap faktorial dengan tiga ulangan untukmasing-masing perlakuan. Di hifosfer aktivitasfosfatase asam lebih tinggi daripada fosfatasealkalin, sedangkan di rhizosfer aktivitas fosfatasealkalin lebih tinggi dibandingkan dengan aktivitasfosfatase asam. Aktivitas fosfatase asam dirhizosfer bibit kelapa sawit yang diinokulasi A.tuberculata nyata lebih tinggi dibandingkandengan bibit yang tidak diinokulasi. Aktivitasfosfatase asam dan fosfatase alkalin sedikit lebihtinggi dengan inokulasi CMA. Sebaliknya,produksi asam organik antara bibit yangdiinokulasi dan bibit yang tidak diinokulasi tidakberbeda nyata. Tampak bahwa simbiosis CMAdengan kelapa sawit lebih meningkatkanmineralisasi P organik dan kurang meningkatkanpelarutan P anorganik.
POTENSI DAERAH SIDIK JARI SPEKTRUM INFRAMERAH SEBAGAI PENANDA BIOAKTOVITAS EKSTRAK TANAMAN OBAT Darusman, Latifah Kosim; Heryanto, Rudi; Rafi, Mohamad; Wahyuni, Wulan Tri
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 3 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.806 KB)

Abstract

Efficacy and quality of medicinal plant extracts depend on chemical composition therein. Therefore, to ensure its efficacy orquality, the chemical composition of extracts have to be analyzed based on its chemical marker or its chemical pattern. This study tried to develop a method for extracts quality assay based on infrared spectrum in fingerprint region and extracts inhibition activity to xanthine oxidase. Five extracts from kunyit (Curcuma domestica), temulawak ( C xanthorriza), jahe (Zingiber officina/e), temukunci (Boesenbergia pandurata), and cabe jawa (Piper retrofractum) were tested to inhibit xanthine oxidase activity. The bioactive data of extracts and its infrared spectrum was analyzed using PCA and PLS-DA (partial least squarediscriminant analysis). Almost all extracts that were tested showed inhibition activity to xanthine oxidase activity, except the extract of jahe. The result showed that PCA can group all of extract into each of their region. Meanwhile, PLS-DA has shown to be a good prediction model for classifying extracts based on fingerprint region of its mR Spectrum and its biological activity. Keywords: fingerprint, mR spectra, compound marker, xanthine oxidase
GEOGRAPHICAL CLASSIFICATION OF JAVA TEA (ORTHOSIPHON STAMINEUS) FROM JAVA ISLAND BY FTIR SPECTROSCOPY COMBINED WITH CANONICAL VARIATE ANALYSIS Rafi, Mohamad; Purwakusumah, Edy Djauhari; Ridwan, Taopik; Barus, Baba; Sutandi, Atang; Darusman, Latifah Kosim
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 1 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.469 KB)

Abstract

FTIR spectroscopy combined with canonical variate analysis was used for differentiation of java tea (Orthosiphon stamineus) according to their geographical origin. FTIR spectra of all java tea samples were acquired in the mid infrared region (wavenumber range 4000-400 cm-1). Preprocessing signal of FTIR spectra has been carried out prior to canonical variate analysis by standard normal variate. Combination of FTIR spectra in the region 1800-900 cm-1with canonical variate analysis has the power to differentiate java tea samples in terms of geographical origin. The developed method could be used for identification of geographical origin of java tea based on the samples used in this study.
INHIBISI LIPASE PANKREAS SECARA IN VITRO OLEH EKSTRAK AIR DAN ETANOL DAUN ASAM JAWA (TAMARINDUS INDICA) DAN RIMPANG KUNCI PEPET (KAEMPFERIAE ROTUNDAE) Pradono, Dyah Iswantini; Darusman, Latifah Kosim; Susanti, Ai
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.355 KB) | DOI: 10.31258/jnat.13.2.146-154

Abstract

Tamarind and kunci pepet has been used traditionally as herbal medicine to reduce body weight or used asantiobesity. But the mechanism in vitro of this herbal in reducing body weight has not been known yet. Theobjective of this research is to evaluate these herbal as antiobesity by their water and ethanol extracts capabilityin inhibiting pancreatic lipase activity in vitro at pH 8, incubation time 45 minutes, and temperature 400C. Pancreaticlipase used in this research was human pancreatic lipase with concentration of 1.4 × 10-5 ?g/?l and the substratewas sesame oil with concentration of 16.2 ?g/?l. The water and ethanol extracts of tamarind leaves containedalkaloids, flavonoids, saponins, steroids, and tannins. Water extract of kunci pepet contained alkaloids, flavonoids,saponins, and tannins, while ethanol extract contained alkaloids, flavonoids, saponins, and triterpenoids. Theresults of tamarind leaves extracts showed that ethanol extract in concentration of 150 ppm had the highestinhibitory effect, with the value of 49.0%. Water extract of kunci pepet at concentration of 200 ppm had the highestinhibition, with the value of 65.1%. These values were higher than inhibitory effect of Xenical® 100 ppm as thepositive control, with the inhibition value of 10.6%.
THE SIDE EFFECT OF THE MELASTOMA MALABATHRICUM L ETHANOL EXTRACT ON THE GONAD MATURATION OF FEMALE ORANGE MUD CRAB (SCYLLA OLIVACEA) Farizah, Nuril; Zairin JR, Muhammad; Darusman, Latifah Kosim; Boediono, Arief; Suprayudi, Muhammad Agus
HAYATI Journal of Biosciences Vol. 25 No. 4 (2018): October 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (944.578 KB) | DOI: 10.4308/hjb.25.4.188

Abstract

The medicinal plants in aquaculture are currently being studied quite extensively by researchers. Medicinal plants can act as an inducer or aphrodisiac and an antifertility agent in gonad maturation. The aimed of the experiment was to known side effects of the herb M. malabathricum L. on maturation process in female mud crab (S. olivacea). This study used 40 crabs with an average weight of 200-250g. The experiment consists of two treatment groups. The controls group (A) that received 100 ?l of aquadest and group treatment (B) received 100 ?l of 2 mg/g of ethanol extract M. malabathricum L. were studied. For 5, 10, 15, and 20 days of experiment, crabs were anesthetized and sacrified. The ovary was collect for GSI and histology. The end of experiment days (20 day), the hemolymph was collect to used ELISA assay. The result showed, significant differences in treatment group and control groups (p<0.05) in terms the macroscopic morphology of ovary, the mean GSI and the diameter of oocytes. Histological showed the influence of extract M. malabathricum L. on inhibited the ovary development compared with the control groups. Based on this results, it is concluded that ethanol extract of M. malabathricum L. leaves at a dose of 2 mg/g act as an antifertility agent for ovary maturation of S. olivacea.
SENYAWA PENCIRI EKSTRAK DAUN JATI BELANDA (GUAZUMA ULMIFOLIA LAMK) SEBAGAI ANTI-KOLESTEROL Batubara, Irmanida; Husnawati, .; Darusman, Latifah Kosim; Mitsunaga, Tohru
Jurnal Ilmu Pertanian Indonesia Vol. 22 No. 2 (2017): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.675 KB) | DOI: 10.18343/jipi.22.2.87

Abstract

Jati belanda (Guazuma ulmifolia Lamk) leaves is traditionally used as slimming and cholesterol reducing agent. This research aims to determine the active component on jati belanda leaves as marker compound for cholesterol reducing agent. The quality of leaves was checked prior to use for next step of analysis. Jati belanda leaves with good quality was extracted by ethanol 30% and the extract was separated by chromatography technique such as open column chromatography and preparative high performance liquid chromatography. The results showed that the quality of samples is meet to the requirement of Indonesian Food and Drug Agency (Badan Pengawas Obat dan Makanan (BPOM)) based on moisture content, ash content, and heavy metal content. Based on the separation results showed that the cholesterol reducing compound from jati belanda is quercetin
CHARACTERIZATION OF AN ENDOPHYTIC BACTERIUM G062 ISOLATE WITH BENEFICIAL TRAITS AKHDIYA, ALINA; WAHYUDI, ARIS TRI; MUNIF, ABDUL; DARUSMAN, LATIFAH KOSIM
HAYATI Journal of Biosciences Vol. 21 No. 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1475.961 KB) | DOI: 10.4308/hjb.21.4.187

Abstract

An endophytic bacterium isolate G062 was characterized base on its molecular genetic potents, morphology, physiology, and biochemistry reactions. Analysis of 16S rDNA sequences of G062 showed the highest similarity to Paracoccus halophilus (98%). Detection of the phlD and prnC genes occurrence indicated that the bacterium had this antibiotic-like genes of Diacethylphloroglucinol (DAPG) and pyrrolnitrin. The cells are rod shaped (0.59-0.89 x 1.85-3.3 µm), aerobic, Gram negative, non motile, non spore forming,  positive catalase, positive oxydase, could reduce NO3 to N2, nitrogen fixing, producing siderophore and plant growth hormones-like compounds (IAA, Gibberellin, and zeatin), and solubilizing phosphate. The G062 isolate could grow on media containing 2.5% NaCl. Range of the temperature and pH growth were 15-40 and 5.0-9.5 oC, respectively. The bacterium did not cause red blood cells lysis. There was no hypersensitive response when it was injected into tobacco leaves, and it was not pathogenic against potato plantlets.  Moreover, the bacterium promoted the growth of the potato plant and had high colonization ability. These results suggested that the bacterium had beneficial and good traits as biological agent candidate to promote potato plant growth.
DIVERSITY OF ENDOPHYTIC FUNGI FROM RED GINGER (ZINGIBER OFFICINALE ROSC.) PLANT AND THEIR INHIBITORY EFFECT TO FUSARIUM OXYSPORUM PLANT PATHOGENIC FUNGI GINTING, ROHANI CINTA BADIA; SUKARNO, NAMPIAH; WIDYASTUTI, UTUT; DARUSMAN, LATIFAH KOSIM; KANAYA, SIHEGIKO
HAYATI Journal of Biosciences Vol. 20 No. 3 (2013): September 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.046 KB) | DOI: 10.4308/hjb.20.3.127

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Indonesia has been known as a country with high medicinal plant diversity. One of the most common medicinal plant from Indonesia is red ginger (Zingiber officinale Rosc.). Nevertheless, limited studies of endophytic fungi associated with these medicinal plants are hitherto available. The objectives of this research were to study the diversity of endophytic fungi on red ginger and to analyze their potential as a source of antifungal agent. All parts of plant organs such as leaf, rhizome, root, and stem were subjected for isolation. Fungal identification was carried out by using a combination of morphological characteristic and molecular analysis of DNA sequence generated from ITS rDNA region. Thirty endophytic fungi were successfully isolated from leaf, rhizome, root, and stem of red ginger plant. Antagonistic activity was tested against Fusarium oxysporum, a pathogenic fungus on plants, using an antagonistic assay. Based on this approach, the fungi were assigned as Acremonium macroclavatum, Beltraniella sp., Cochliobolus geniculatus and its anamorphic stage Curvularia affinis, Fusarium solani, Glomerella cingulata, and its anamorphic stage Colletotrichum gloeosporoides, Lecanicillium kalimantanense, Myrothecium verrucaria, Neonectria punicea, Periconia macrospinosa, Rhizopycnis vagum, and Talaromyces assiutensis. R. vagum was found specifically on root whereas C. affinis, L. kalimantanense, and M. verrucaria were found on stem of red ginger plant.  A. macroclavatum was found specifically in red ginger plant?s organ which located under the ground, whereas C. affinis was found from shoot or organ which located above the ground. The antagonistic activity of isolated endophytic fungi against F. oxysporum varied with the inhibition value range from 1.4 to 68.8%. C. affinis (JMbt7), F. solani (JMd14), and G. cingulata (JMr2) had significantly high antagonistic activity with the value above 65%; and R. vagum (JMa4) and C. geniculatus (JMbt9) had significantly low antagonistic activity with the range value 0-10%.
ZINGIBER CASSUMUNAR ROXB. (BANGLE), GUAZUMA ULMIFOLIA LAMK. (JATI BELANDA), AND MURRAYA PANICULATA (KEMUNING) HAVE BEEN USED AS SLIMMING AGENTS IN JAMU. A FEW RESEARCHES HAVE PERFORMED STUDIES ON THEIR POTENCY AS ANTIOBESITY. THE AIM OF THIS RESEARCH WAS TO INVESTIGATE THE POTENCY OF Z. CASSUMUNAR RHIZOME, G. ULMIFOLIA, AND M. PANICULATA LEAF EXTRACTS AS ANTIOBESITY AGENT BASED ON IN VITRO INHIBITION ACTIVITY OF THE EXTRACTS ON PANCREATIC LIPASE ACTIVITY. IN THIS RESEARCH, WATER CONTENT DETERMIN ISWANTINI, DYAH; SILITONGA, RHOITO FROISTA; MARTATILOFA, ELIZABETH; DARUSMAN, LATIFAH KOSIM
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.6

Abstract

Zingiber cassumunar Roxb. (Bangle), Guazuma ulmifolia Lamk. (Jati belanda), and Murraya paniculata (Kemuning) have been used as slimming agents in jamu. A few researches have performed studies on their potency as antiobesity. The aim of this research was to investigate the potency of Z. cassumunar rhizome, G. ulmifolia, and M. paniculata leaf extracts as antiobesity agent based on in vitro inhibition activity of the extracts on pancreatic lipase activity. In this research, water content determination, phytochemical assay, toxicity assay and in vitro assay of inhibition activity on pancreatic lipase were performed toward single and mixture extracts of Z. cassumunar, G. ulmifolia, and M. paniculata resulted by water, ethanol, and saponin extractions. The results indicated that 100 ppm of ethanol extraction of Z. cassumunar had highest inhibition effect on the activity of pancreatic lipase (29.17%), followed by 100 ppm of water extraction of M. paniculata  (25.66%), 60 ppm of ethanol extraction of G. ulmifolia leaves (25.13%) and ethanol extraction mixture of Z. cassumunar, G. ulmifolia, and M. paniculata  leaves with ratio of 25:25:25 (21.58%). These inhibition effects were higher than inhibitory effect of 100 ppm of Xenical®/orlistat as the positive control, with the inhibition value of 17.53%. Saponin crude extracts had lower inhibitory effect than the other extractions. It was suggested that ethanol extraction of Z. cassumunar, and G. ulmifolia and water extraction of M. paniculata had potency as antiobesity agent
AKTIVITAS PENGHAMBATAN α-GLUCOSIDASE OLEH KOMBINASI EKSTRAK ETANOLIK BUAH Phaleria macrocarpa (SCHEFF.) BOERL. DAN DAUN Annona muricata LINN. Rahmi, Era; Wahyuni, Wulan Tri; Darusman, Latifah Kosim; Suparto, Irma Herawati
Majalah Obat Tradisional Vol 21, No 2 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (703.714 KB) | DOI: 10.22146/tradmedj.12819

Abstract

Buah Phaleria macrocarpa dan daun Annona muricata telah dilaporkan memiliki aktivitas antidiabetes dengan menginhibisi aktivitas enzim α-glukosidase. Masing-masing tanaman herbal tersebut menunjukkan aktivitas inhibisi yang rendah terhadap enzim α-glukosidase. Kombinasi dua tanaman ini diharapkan dapat memberikan efek sinergis. Penelitian ini dilakukan untuk mengevaluasi aktivitas inhibisi enzim α-glukosidase gabungan ekstrak etanol buah P. macrocarpa dan daun A. muricata dan mengidentifikasi profil kromatografi lapis tipis (KLT) masing-masing buah P. macrocarpa, daun A. Muricata dan gabungan ekstrak yang memiliki aktivitas terbaik. Ekstraksi dilakukan dengan teknik maserasi menggunakan etanol 96%. Kombinasi ekstrak gabungan diformulasi berdasarkan nilai IC50 dari masing-masing ekstrak. Hasil analisis menunjukkan bahwa aktivitas inhibisi bergantung pada konsentrasi, dengan nilai IC50 masing-masing ekstrak buah P. macrocarpa dan daun A. Muricata secara berturut-turut adalah 261.343 dan 428.790 µg/mL. Gabungan ekstrak yang memiliki aktivitas inhibisi tertinggi (84.52±0.79%) terdiri dari gabungan ekstrak buah mahkota dewa pada konsentrasi IC50 dan ekstrak daun sirsak pada konsentrasi 2/3 IC50. Berdasarkan profil KLT, terdeteksi adanya senyawa bioaktif pada ekstrak gabungan yang memiliki aktivitas paling baik, yang mana senyawa tersebut juga terdeteksi pada masing-masing ekstrak. Hal ini dapat diasumsikan bahwa dengan meningkatnya kandungan senyawa fitokimia yang terkandung dalam gabungan ekstrak dapat dihasilkan suatu efek sinergis.