Articles

UJI ADAPTASI MULTIMUSIM KARAKTER FENOTIP KULTIVAR MELODI GAMA 3 (CUCUMIS MELO L.): USAHA PENGUATAN INDUSTRI BENIH NASIONAL Sidiq, Yasir; Maryanto, Sigit Dwi; Daryono, Budi Setiadi
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

Tanaman melon (Cucumis melo  L.) merupakan tanaman pertanian yang banyak dibudidayakan untuk konsumsi buahnya. Salah satu permasalahan dalam budidaya melon adalah benih yang diperoleh secara impor sehingga harganya relatif mahal. Perakitan kultivar MelodiGama 3 (MG3) merupakan strategi untuk mengurangi ketergantungan terhadap benih impor. Kultivar MG3 merupakan hasil persilangan antara kultivar ? MG1 dengan ? La-3 (tahun 2011). Tujuan penelitian ini untuk mengetahui kemampuan adaptasi kultivar MG3 terhadap kondisi lingkungan pada musim kemarau (tahun 2012) dan musim penghujan (tahun 2013) dan mengamati kestabilan kultivar MG3 di kedua musim tersebut di demplot milik Kebun Pendidikan Penelitian dan Pengembangan Pertanian (KP4) Universitas Gadjah Mada (UGM), Yogyakarta. Hasil yang diperoleh menunjukkan bahwa karakter melon MG3 dari penanaman musim penghujan umumnya lebih unggul daripada penanaman musim kemarau. Melon MG3 penanaman musim penghujan unggul pada karakter berat buah, keliling buah, ukuran buah, dan berat biji. Namun terdapat penurunan karakter unggul yaitu tingkat kemanisan (brix) dari 11,53% menjadi 9,98%. Hasil tersebut terjadi karena musim penghujan yang mempengaruhi kadar air dalam buah sehingga tingkat kemanisanya menurun. Kata kunci: MG3, Karakter Fenotip, Pemuliaan Tanaman
PERFORMA FENOTIPIK KAMBRO HASIL PERSILANGAN ANTARA BETINA BROILER COBB 500 DAN JANTAN PELUNG BLIRIK HITAM Mahardhika, I Wayan Swarautama; Daryono, Budi Setiadi
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2019.v11.i02.p12

Abstract

Riset ini diadakan dengan tujuan mengukur performa fenotipik Kambro hasil persilangan antara Pelung Blirik Hitam dan Broiler Cobb 500. Berdasarkan pengukuran Bobot Tubuh (BT), rerata BT Kambro (n = 17) mencapai 1.244,14 ± 453,82 gram signifikan (p<0,001) terhadap F1 Pelung (n = 7) dengan rerata BT 602,88 ± 79,93 gram pada umur 8 minggu dengan diet pakan standar ad libitum. Performa fenotipik Kambro signifikan terhadap F1 Pelung berdasarkan parameter bobot tubuh linear, parameter vitalitas, parameter PPa-PBe dan parameter fenotipe. Kambro memiliki perpaduan fenotip indukannya berdasarkan parameter fenotipe. Parameter PPa merupakan model estimasi BT Kambro berdasarkan regresi non-linear quadratic (r = 0,956) dengan formula 1.84E3 ± 3.54E2*x+31.73*x2. Perbedaan grup antar grup signifikan (p<0,014) terhadap BT dan tidak terdapat interaksi antara grup dan parameter bobot tubuh linear berdasarkan analisis kovarian. Tingkat mortalitas Kambro lebih rendah dibandingkan F1 Pelung tanpa vaksinasi dengan sistem pemeliharaan semi-intensif. Sebagai akibat dari ukuran populasi hibrida terbatas, temuan riset harus divalidasi dengan ukuran populasi hibrida lebih besar.
POLA PEWARISAN CREST AYAM (GALLUS GALLUS DOMESTICUS, LINNAEUS 1758) BACKCROSS HASIL PERSILANGAN AYAM MAHKOTA DENGAN AYAM KAMPUNG Daryono, Budi Setiadi; Puspita, Utin Elsya
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.33 KB) | DOI: 10.22146/jsv.17884

Abstract

AbstrakMemelihara ayam hias merupakan salah satu kegemaran masyarakat Indonesia dan juga saranameningkatkan pendapatan. Salah satu ayam hias yang unik dan banyak menarik minat pecinta ayam hias adalah ayam Mahkota yang memiliki ciri khas bulu yang tersusun lebat di bagian kepala hingga menutupi mata. Jenis ayam hias tersebut umumnya memiliki kelemahan yaitu daya tahannya yang rendah terhadap penyakit. Ayam Kampung atau ayam buras merupakan ayam lokal asli Indonesia yang memiliki keunggulan yaitu memiliki ketahanan yang lebih tinggi terhadap penyakit dibanding ayam ras. Penggabungan keunikan crest dari ayam Mahkota dan ketahanan terhadap penyakit dari ayam Kampung dapat diwujudkan salah satunya melalui proses backcross (BC). Penelitian ini dilakukan dengan menyilangkan ayam F Mahkota betina hasil persilangan ayam 1 Mahkota dan ayam Kampung dengan ayam Mahkota jantan. Tujuan penelitian ini adalah untuk mempelajari pola pewarisan crest dan pertumbuhan bobot keturunan BC pada umur 7 minggu. Anakan yang dihasilkan diamati perkembangan crest dan pertambahan bobotnya selama 7 minggu, kemudian variasi fenotipnya dianalisis dengan chi square test. Hasil penelitian menunjukkan terdapat 5 kelas fenotip yang berbeda dari 16 individu keturunan BC yang menunjukkan gejala poligen dengan 2 gen yang mempengaruhi dan memenuhi pola pewarisan 1:4:6:4:1.
STUDI ETNOBOTANI DIOSCOREA SPP. (DIOSCOREACEAE) DAN KEARIFAN BUDAYA LOKAL MASYARAKAT DI SEKITAR HUTAN WONOSADI GUNUNG KIDUL YOGYAKARTA Purnomo, Purnomo; Daryono, Budi Setiadi; Rugayah, Rugayah; Sumardi, Issirep
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.947 KB) | DOI: 10.31258/jnat.14.3.191-198

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Dioscorea spp. were cultivated as alternative food in the garden and yard on dry season, and it?s planted by peoples in the villages. The objectives of the research are to identify the species, the tuber use, and the local wisdom to support the conservation of Dioscorea around Wonosadi forest. Samples and data collection was conducted in 15 villages around Wonosadi forest. Interview survey was done on peoples who are cultivate yam species (Dioscorea spp.) in their garden. Research were emphasized to tuber characteristic, traditional uses, and local wisdom to supporting yam conservation. Data was analyzed descriptively to illustrate the kind of local usage and local wisdom of connect with Dioscorea spp. The results showed that there were found species of gembili (D. esculenta (Lour.) Burk.), uwi (D. alata L.), and gadung (D. hispida dennst.) as cultivated plants, and tomboreso (D. pentaphylla L.), gembolo (D. bulbifera L.), and jebubuk (D. numularia Lamk.) as wild species in the forest. Tuber is raw material for many kind of food based on carbohydrate content. Gadung were used as fickle food with high diversification and insecticide for rice and corn. Peoples more consume gembili and uwi than gembolo and others species as rice substitute at dry season. The local wisdom were indicated that peoples still consume gembili and uwi as rice substitute, while tuber also used as sesaji in traditional concept jajan pasar or tukon pasar, and tuber of uwi was used as diet for diabetes complaint, and row material of uwi ungu tuber was used effectively for diarrhea complaint in the villages.
PROFIL PROTEIN STADIUM SPOROZOIT EIMERIA TENELLA ISOLAT YOGYAKARTA MELALUI ANALISIS PROTEIN SDS-PAGE (PROTEIN PROFILE OF THE SPOROZOITE OF EIMERIA TENELLA ISOLATES FROM YOGYAKARTA USING SDS-PAGE PROTEIN ANALYSIS) Tresnani, Galuh; Prastowo, Joko; Nurcahyo, Wisnu; Daryono, Budi Setiadi
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of thedisease is between 80 to 90%. A rapid and prompt diagnosis would be one of the essential steps ineradication and control of the disease. The objective of this study is to determine the protein profile ofsporozoite of Eimeria tenella isolated in Yogyakarta using sodium dodecyl sulphate polyacrilamide gelelectrophoresis (SDS-PAGE) protein analysis. Protein analysis was performed in 12% polyacrilamide geland further electrophoresis at 100 volts and over-staining with Coomasie brilliant blue. The resultsshowed that the sporozoite of E. tenella isolated in Yogyakarta contained five proteins with molecularweights of 15, 26, 32, 80, and 91 kDa, respectively.
Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon Daryono, Budi Setiadi; Aristya, Ganies Riza; Kasiamdari, Rina Sri
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.   Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5
Application of Multiplex RT-PCR for Detection of Cucurbit-infecting Tobamovirus Daryono, Budi Setiadi; Natsuaki, Keiko T.
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

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Cucumber green mottle mosaic virus (CGMMV) and Kyuri green mottle mosaic virus (KGMMV) are seed borne viruses and they are also transmitted mechanically during agricultural practice and through water. Hence, these viruses have potential diseases widely distributed throughout the world. To detect different strains of CGMMV and KGMMV, several specific primers for each virus were designed for single and multiplex RT-PCR. The results of single and multiplex RT-PCR showed that CGMMV was detected in zucchini isolated in Bali-Indonesia, while KGMMV was detected both in zucchini isolated in Bali-Indonesia and Cucumis metuliferus isolated in Thailand. Furthermore, artificial co-infection of these two viruses was prepared and carried out using two different ways of viral RNAs extraction. Based on the results, it could be reported that viral RNAs for cDNA amplification by multiplex RT-PCR could be extracted from a mixture of infected leaves or separate extraction of each viruses infected leaves. In addition, results presented in this study demonstrated the application of multiplex RT-PCR to simultaneously detect CGMMV and KGMMV from cucurbit leaves using a mixture of four primers and its feasibility as a sensitive and rapid laboratory assay. Since, no multiplex RT-PCR technique has been described for the detection of CGMMV and KGMMV, this technique can be a good option for sensitive and reliable tool for detection of two major cucurbit infecting Tobamoviruses.Keywords : Cucurbit infecting Tobamovirus, multiplex RT-PCR, seed borne viruses
Genetic Variation Analysis of Mold (Magnaporthe oryzae B.Couch) Using Random Amplified Polymorphic DNA Pramono, Ajeng Kusumaningtyas; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Magnaporthe oryzae B.Couch is a host-specific fungi, certain strain only infect certain host plant species. Genetic variety among M. oryzae isolates was explained by dendogram which was constructed using similarity data of Random Amplified Polymorphic DNA (RAPD). Dendogram construction was achieved by computer software, Numerical Taxonomy System (NTSYS). The aim of the research were to study the genetic variation among M. Oryzae using RAPD and to construct a dendogram of genetic similarities among the ten isolates from green foxtail (Setaria viridis L.), finger millet (Eleusine coracana L.) and rice (Oryza sativa L.).RAPD was performed in 30 cycles using 5 primers (OPA-02, OPA-03, OPA-04, OPA-05, OPA-07). Polymorphism data was used to constructed dendogram using Dice index and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) in NTSYS software. There were 68 polymorphism fragments from 74 amplified fragments.Three clusters were formed in the dendrogram, based on host pathotype: foxtail millet type, finger millet type and rice type. There were two subclusters in foxtail millet type based on mating type, MAT1-1 dan MAT1-2. Thus, RAPD could be used as a method for genetic variation analysis of Magnaporthe oryzae to show host-specific specificity.Key words: Magnaporthe oryzae, RAPD, mating type
The genetic variations and relationship of Madura tobacco (Nicotiana tabacum L.) based on molecular characteristics Nadifah, Fitri; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 21, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1026.99 KB) | DOI: 10.22146/ijbiotech.10582

Abstract

Madura has at least 22 genotypes of local tobaccos (Nicotiana tabacum L.). This diversity could potentially produce new genotype of tobaccos with superior characters. However, information of the genetic diversity of Madura tobaccos is still limited. The aim of this study was to determine the genetic variation and relationship of 24 genotypes of Madura tobaccos with Random Amplified Polymorphic DNA (RAPD) analysis. In this research we were used 6 single primers for amplification: (OPA-18, OPB-12, OPB-14, OPC-1, OPC-8 and OPC-19) and 2 mixture primers ((OPB-12+OPC-8) and (OPC-1+OPC-19)). Genetic similarity and clustering was analyzed with Unweighted Pair Group Method Arithmetic (UPGMA) method with Numerical Taxonomy and Multivariate Analysis System (NTSYS) version 2.10 software. From this research we found that OPA18425, OPB12450, OPC8500, (OPC19+OPC1)550 and OPC8800 can be used as specific markers. Polymorphic bands percentage with mixture primers was relatively equal with single primers (<60%). The dendogram showed that Madura tobacco genotypes consist of 2 main clusters: cluster A (22 genotypes) and cluster B (2 genotypes: Bukabu Sa’ang and Prancak-95). Madura tobaccos had high genetic similarity between genotypes ranging from 0.80-1.00.
CHROMOSOME CHARACTERIZATION OF THREE VARIETIES OF GINGER (Zingiber officinaleRosc.) Daryono, Budi Setiadi; Rahma, Siti Nur Azizah Fauziati; ., Purnomo; ., Sudarsono
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.764 KB) | DOI: 10.14499/indonesianjpharm0iss0pp54-59

Abstract

Giant  ginger  (Zingiber  officinale Rosc.  var.  officinale),  red ginger  (Zingiber  officinale Rosc.  var.  rubra)  and  small  ginger (Zingiber  officinale Rosc.  var.  amarum)  are  three  varieties  of Zingiber officinale Rosc. They have a lot of benefit and often used by  Indonesian  as  a  traditional  drug.  Moreover,  they  have  a  big chance to be use as a flavor in world wide. Therefore, research for their  quality,  quantity  and  continuity  of  supplyare  needed. Characterization  of  their  chromosomes  is  one  effort for  improving ginger  cultivation.  The  objective  of  this  research  was  to  study mitotic  time  and  chromosome  characters  of  three  varieties  of ginger.  Squashing method was used for chromosome preparation. The  results  showed  that  mitotic  time  of  giant  ginger  is  09.00-10.05  am,  red  ginger  is  09.00-10.30  am,  while  small  ginger  is 08.45-11.00  am.  Chromosome  number  of  giant  ginger  and  small ginger are 2n=2x=30, while red ginger is 2n=2x=22. Giant ginger has R= 3,109, Red ginger has R = 3,206 and small ginger has R = 4,065.  Based  on  chromosome  characters  it  is  revealed  that relationship  between  giant  ginger  and  red  ginger  is  closer  that  of compare  to  small  ginger.  This  result  is  important  as  basic information for improving the gingers production through breeding program.Key  words:  Zingiber  officinale Rosc.,  mitotic  time,  chromosome characterization, squashmethod