Articles

Karakter Fenotip Jahe Merah (Zingiber officinale Roxb. var rubrum Rosc.) Hasil Poliploidisasi dengan Kolkisin Friska, Meiliana; Daryono, Budi Setiadi
Al-Kauniyah: Jurnal Biologi Vol 10, No 2 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (649.735 KB) | DOI: 10.15408/kauniyah.v10i2.4813

Abstract

ABSTRAKJahe merah (Zingiber officinale Roxb. var. rubrum Rosc.) merupakan salah satu tanaman jamu-jamuan yang banyak digunakan sebagai bumbu masakan, bahan obat tradisional, manisan, minuman penyegar, dan sebagai bahan komoditas ekspor nonmigas. Rimpang pada jahe memiliki manfaat untuk kesehatan kesegaran, dan campuran untuk membuat masakan. Rasa pedas yang ditimbulkan pada jahe disebabkan adanya senyawa keton ‘gingerol’. Penelitian ini bertujuan untuk mendapatkan tanaman poliploid pada jahe merah dengan mengamati perubahan karakter fenotip pada daun, batang dan rimpang hasil induksi kolkisin. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Kelompok (RAK) pola faktorial dengan dua faktor yaitu konsentrasi kolkisin (0,05%, 0,1%, and 0,2%) dan waktu perendaman (6, 12, dan 24 jam). Hasil pengamatan dianalisis dengan Analysis of Variance (ANOVA) dan pengolahan data secara statistik dilakukan dengan menggunakan uji F pada taraf α = 5%. Jika menunjukkan perbedaan nyata dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa perubahan karakter fenotip jahe merah hasil induksi kolkisin terlihat pada perlakuan K1W2 (konsentrasi 0,05% lama perendaman 12 jam). Terlihat pada tinggi tanaman, diameter batang semu, jumlah daun, berat rimpang, dan kadar klorofil sedangkan pada panjang daun, lebar daun dan luas daun tidak menunjukkan perbedaan secara signifikan. ABSTRACT Red ginger (Zingiber officinale Roxb. var. rubrum Rosc.) is one of the herb plants that often being used as a spice, traditional medicines, sweets, drinks, and material export commodities. The ginger’s rhizome is the most common because it is good for health freshness, and spice for food. The spicy from the ginger comes from ketone compounds ‘gingerol’. This research aimed to produce polyplody crops in red ginger by observing the changes of phenotype characters on the leaves, stems and rhizome resulted from colchicine induction. This research was based on experimental method with Randomized design with two factors, which were colchicine concentration (0,05%, 0,1% and 0,2%) and incubation time (6, 12 and 24 hours). The data were analyzed using Analysis of Variance (ANOVA) and statistical data processing was done using F test at α = 5%. If the result shows any significant differences then was followed with Duncan Multiple Range Test (DMRT). The results showed that the change in the phenotype characters of red ginger was seen in K1W2 (0,05 % concentration of incubation time 12 hours). Phenotype characters include plant height, stem diameter, number of leaves, rhizome weight and leaf chlorophyll content showed phenotypic characters change significantly while leaf length, leaf width and leaf area did not indicate any change in the character of phenotypes significantly. 
UJI ADAPTASI MULTIMUSIM KARAKTER FENOTIP KULTIVAR MELODI GAMA 3 (CUCUMIS MELO L.): USAHA PENGUATAN INDUSTRI BENIH NASIONAL Sidiq, Yasir; Maryanto, Sigit Dwi; Daryono, Budi Setiadi
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

Tanaman melon (Cucumis melo  L.) merupakan tanaman pertanian yang banyak dibudidayakan untuk konsumsi buahnya. Salah satu permasalahan dalam budidaya melon adalah benih yang diperoleh secara impor sehingga harganya relatif mahal. Perakitan kultivar MelodiGama 3 (MG3) merupakan strategi untuk mengurangi ketergantungan terhadap benih impor. Kultivar MG3 merupakan hasil persilangan antara kultivar ? MG1 dengan ? La-3 (tahun 2011). Tujuan penelitian ini untuk mengetahui kemampuan adaptasi kultivar MG3 terhadap kondisi lingkungan pada musim kemarau (tahun 2012) dan musim penghujan (tahun 2013) dan mengamati kestabilan kultivar MG3 di kedua musim tersebut di demplot milik Kebun Pendidikan Penelitian dan Pengembangan Pertanian (KP4) Universitas Gadjah Mada (UGM), Yogyakarta. Hasil yang diperoleh menunjukkan bahwa karakter melon MG3 dari penanaman musim penghujan umumnya lebih unggul daripada penanaman musim kemarau. Melon MG3 penanaman musim penghujan unggul pada karakter berat buah, keliling buah, ukuran buah, dan berat biji. Namun terdapat penurunan karakter unggul yaitu tingkat kemanisan (brix) dari 11,53% menjadi 9,98%. Hasil tersebut terjadi karena musim penghujan yang mempengaruhi kadar air dalam buah sehingga tingkat kemanisanya menurun. Kata kunci: MG3, Karakter Fenotip, Pemuliaan Tanaman
PERFORMA FENOTIPIK KAMBRO HASIL PERSILANGAN ANTARA BETINA BROILER COBB 500 DAN JANTAN PELUNG BLIRIK HITAM Mahardhika, I Wayan Swarautama; Daryono, Budi Setiadi
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This research was conducted to measure the phenotypic performance of Kambro crossbreeds of Pelung Blirik Hitam and Broiler Cobb 500. Based on Body Weight (BT) measurement, Kambro population (n = 17) has an average BT of 1,244.14 ± 453.82 grams significant (p <0.01) to F1 Pelung (n = 7) with an average BT of 602.88 ± 79.93 grams in 8 weeks period with ad libitum diet of standard feed. Phenotypic performance of Kambro significant to F1 Pelung based on the measurement of linear bodyweight parameter, vitality parameter, PPa-PBe parameter and phenotype parameter. Kambro has the phenotype combination of parental generation based on phenotype parameter. PPa parameter was suitable BT estimation model based on non-linear quadratic regression (r = 0.956) with formula 1.84E3 ±3.54E2*x+31.73*x2. Difference between chicken group (p<0.014) was significant to BT and interaction between group and linear bodyweight parameter was not significant based on Analysis of Covariance. The mortality rate of Kambro was lower than F1 Pelung with the absent of vaccination in semi-intensive rearing system. As the size of hybrids population was limited, research findings must be validated with larger population size of hybrids.
Pola Pewarisan Crest Ayam (Gallus gallus domesticus, Linnaeus 1758) Backcross Hasil Persilangan Ayam Mahkota dengan Ayam Kampung Daryono, Budi Setiadi; Puspita, Utin Elsya
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.33 KB) | DOI: 10.22146/jsv.17884

Abstract

AbstrakMemelihara ayam hias merupakan salah satu kegemaran masyarakat Indonesia dan juga saranameningkatkan pendapatan. Salah satu ayam hias yang unik dan banyak menarik minat pecinta ayam hias adalah ayam Mahkota yang memiliki ciri khas bulu yang tersusun lebat di bagian kepala hingga menutupi mata. Jenis ayam hias tersebut umumnya memiliki kelemahan yaitu daya tahannya yang rendah terhadap penyakit. Ayam Kampung atau ayam buras merupakan ayam lokal asli Indonesia yang memiliki keunggulan yaitu memiliki ketahanan yang lebih tinggi terhadap penyakit dibanding ayam ras. Penggabungan keunikan crest dari ayam Mahkota dan ketahanan terhadap penyakit dari ayam Kampung dapat diwujudkan salah satunya melalui proses backcross (BC). Penelitian ini dilakukan dengan menyilangkan ayam F Mahkota betina hasil persilangan ayam 1 Mahkota dan ayam Kampung dengan ayam Mahkota jantan. Tujuan penelitian ini adalah untuk mempelajari pola pewarisan crest dan pertumbuhan bobot keturunan BC pada umur 7 minggu. Anakan yang dihasilkan diamati perkembangan crest dan pertambahan bobotnya selama 7 minggu, kemudian variasi fenotipnya dianalisis dengan chi square test. Hasil penelitian menunjukkan terdapat 5 kelas fenotip yang berbeda dari 16 individu keturunan BC yang menunjukkan gejala poligen dengan 2 gen yang mempengaruhi dan memenuhi pola pewarisan 1:4:6:4:1.
Studi Etnobotani Dioscorea spp. (Dioscoreaceae) dan Kearifan Budaya Lokal Masyarakat di Sekitar Hutan Wonosadi Gunung Kidul Yogyakarta Purnomo, Purnomo; Daryono, Budi Setiadi; Rugayah, Rugayah; Sumardi, Issirep
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.947 KB) | DOI: 10.31258/jnat.14.3.191-198

Abstract

Dioscorea spp. were cultivated as alternative food in the garden and yard on dry season, and it’s planted by peoples in the villages. The objectives of the research are to identify the species, the tuber use, and the local wisdom to support the conservation of Dioscorea around Wonosadi forest. Samples and data collection was conducted in 15 villages around Wonosadi forest. Interview survey was done on peoples who are cultivate yam species (Dioscorea spp.) in their garden. Research were emphasized to tuber characteristic, traditional uses, and local wisdom to supporting yam conservation. Data was analyzed descriptively to illustrate the kind of local usage and local wisdom of connect with Dioscorea spp. The results showed that there were found species of gembili (D. esculenta (Lour.) Burk.), uwi (D. alata L.), and gadung (D. hispida dennst.) as cultivated plants, and tomboreso (D. pentaphylla L.), gembolo (D. bulbifera L.), and jebubuk (D. numularia Lamk.) as wild species in the forest. Tuber is raw material for many kind of food based on carbohydrate content. Gadung were used as fickle food with high diversification and insecticide for rice and corn. Peoples more consume gembili and uwi than gembolo and others species as rice substitute at dry season. The local wisdom were indicated that peoples still consume gembili and uwi as rice substitute, while tuber also used as sesaji in traditional concept jajan pasar or tukon pasar, and tuber of uwi was used as diet for diabetes complaint, and row material of uwi ungu tuber was used effectively for diarrhea complaint in the villages.
Profil Protein Stadium Sporozoit Eimeria tenella Isolat Yogyakarta Melalui Analisis Protein SDS-PAGE (PROTEIN PROFILE OF THE SPOROZOITE OF Eimeria tenella ISOLATES FROM YOGYAKARTA USING SDS-PAGE PROTEIN ANALYSIS) Tresnani, Galuh; Prastowo, Joko; Nurcahyo, Wisnu; Daryono, Budi Setiadi
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of thedisease is between 80 to 90%. A rapid and prompt diagnosis would be one of the essential steps ineradication and control of the disease. The objective of this study is to determine the protein profile ofsporozoite of Eimeria tenella isolated in Yogyakarta using sodium dodecyl sulphate polyacrilamide gelelectrophoresis (SDS-PAGE) protein analysis. Protein analysis was performed in 12% polyacrilamide geland further electrophoresis at 100 volts and over-staining with Coomasie brilliant blue. The resultsshowed that the sporozoite of E. tenella isolated in Yogyakarta contained five proteins with molecularweights of 15, 26, 32, 80, and 91 kDa, respectively.
Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon Daryono, Budi Setiadi; Aristya, Ganies Riza; Kasiamdari, Rina Sri
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.   Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5
Application of Multiplex RT-PCR for Detection of Cucurbit-infecting Tobamovirus Daryono, Budi Setiadi; Natsuaki, Keiko T.
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

Cucumber green mottle mosaic virus (CGMMV) and Kyuri green mottle mosaic virus (KGMMV) are seed borne viruses and they are also transmitted mechanically during agricultural practice and through water. Hence, these viruses have potential diseases widely distributed throughout the world. To detect different strains of CGMMV and KGMMV, several specific primers for each virus were designed for single and multiplex RT-PCR. The results of single and multiplex RT-PCR showed that CGMMV was detected in zucchini isolated in Bali-Indonesia, while KGMMV was detected both in zucchini isolated in Bali-Indonesia and Cucumis metuliferus isolated in Thailand. Furthermore, artificial co-infection of these two viruses was prepared and carried out using two different ways of viral RNAs extraction. Based on the results, it could be reported that viral RNAs for cDNA amplification by multiplex RT-PCR could be extracted from a mixture of infected leaves or separate extraction of each viruses infected leaves. In addition, results presented in this study demonstrated the application of multiplex RT-PCR to simultaneously detect CGMMV and KGMMV from cucurbit leaves using a mixture of four primers and its feasibility as a sensitive and rapid laboratory assay. Since, no multiplex RT-PCR technique has been described for the detection of CGMMV and KGMMV, this technique can be a good option for sensitive and reliable tool for detection of two major cucurbit infecting Tobamoviruses.Keywords : Cucurbit infecting Tobamovirus, multiplex RT-PCR, seed borne viruses
Genetic Variation Analysis of Mold (Magnaporthe oryzae B.Couch) Using Random Amplified Polymorphic DNA Pramono, Ajeng Kusumaningtyas; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Magnaporthe oryzae B.Couch is a host-specific fungi, certain strain only infect certain host plant species. Genetic variety among M. oryzae isolates was explained by dendogram which was constructed using similarity data of Random Amplified Polymorphic DNA (RAPD). Dendogram construction was achieved by computer software, Numerical Taxonomy System (NTSYS). The aim of the research were to study the genetic variation among M. Oryzae using RAPD and to construct a dendogram of genetic similarities among the ten isolates from green foxtail (Setaria viridis L.), finger millet (Eleusine coracana L.) and rice (Oryza sativa L.).RAPD was performed in 30 cycles using 5 primers (OPA-02, OPA-03, OPA-04, OPA-05, OPA-07). Polymorphism data was used to constructed dendogram using Dice index and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) in NTSYS software. There were 68 polymorphism fragments from 74 amplified fragments.Three clusters were formed in the dendrogram, based on host pathotype: foxtail millet type, finger millet type and rice type. There were two subclusters in foxtail millet type based on mating type, MAT1-1 dan MAT1-2. Thus, RAPD could be used as a method for genetic variation analysis of Magnaporthe oryzae to show host-specific specificity.Key words: Magnaporthe oryzae, RAPD, mating type
The genetic variations and relationship of Madura tobacco (Nicotiana tabacum L.) based on molecular characteristics Nadifah, Fitri; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 21, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1026.99 KB) | DOI: 10.22146/ijbiotech.10582

Abstract

Madura has at least 22 genotypes of local tobaccos (Nicotiana tabacum L.). This diversity could potentially produce new genotype of tobaccos with superior characters. However, information of the genetic diversity of Madura tobaccos is still limited. The aim of this study was to determine the genetic variation and relationship of 24 genotypes of Madura tobaccos with Random Amplified Polymorphic DNA (RAPD) analysis. In this research we were used 6 single primers for amplification: (OPA-18, OPB-12, OPB-14, OPC-1, OPC-8 and OPC-19) and 2 mixture primers ((OPB-12+OPC-8) and (OPC-1+OPC-19)). Genetic similarity and clustering was analyzed with Unweighted Pair Group Method Arithmetic (UPGMA) method with Numerical Taxonomy and Multivariate Analysis System (NTSYS) version 2.10 software. From this research we found that OPA18425, OPB12450, OPC8500, (OPC19+OPC1)550 and OPC8800 can be used as specific markers. Polymorphic bands percentage with mixture primers was relatively equal with single primers (<60%). The dendogram showed that Madura tobacco genotypes consist of 2 main clusters: cluster A (22 genotypes) and cluster B (2 genotypes: Bukabu Sa’ang and Prancak-95). Madura tobaccos had high genetic similarity between genotypes ranging from 0.80-1.00.
Co-Authors Agus Hery Susanto Agus Nuryanto Ajeng Kusumaningtyas Pramono, Ajeng Kusumaningtyas Al-Mughni, Eka Wasi? Al-Mughni, Eka Wasi’ Alin Liana, Alin Anak Agung Gde Raka Swastika, Anak Agung Gde Raka Arfa, Namira Nur Ariestya, Ganies Riza Bani, Polikarpia Wilhelmina Damayanti, Putu Ayu DIAN ARUNI KUMALAWATI E. Suharyanto, E. Endang Semiarti Faizatul Fitriyah, Faizatul Fitri Nadifah Friska, Meiliana Galuh Tresnani Ganies Riza Aristya, Ganies Riza Ganies Rizaa Aristya, Ganies Rizaa Hanini, Hanifa Husnudin, Uni Baroroh I Wayan Suardana Ignatius Julijantono, Ignatius Indraningsih, Estiyani Irma Nofitahesti, Irma Ishak, Muhammad Alif Issirep Sumardi Iwan Roosdianto Jang, Soenghoe Joko Prastowo Keiko T. Natsuaki, Keiko T. Keiko T. Natsuaki`, Keiko T. KENJI WAKUI Lawrie, Muhammad Dylan Linda Oktavianingsih, Linda Mahardhika, I Wayan Swarautama Mahfut Mahfut, Mahfut Maryani Maryani Miftahul Mushlih, Miftahul Muhammad Taufiq Qurrohman, Muhammad Taufiq Nofriarno, Nugroho Nofriarno, Nugroho Parazulfa, Anisa Perdamaian, Ayudha Bahana I. Prabawani, Ratri Lila Purnomo . Purnomo Purnomo Puspita, Utin Elsya RABBANI, ANNAS REFLINUR REFLINUR Ridesti Rindyastuti Rina Sri Kasiamdari Rugayah Rugayah Saputri, Avia Purnama Saragih, Hendry Tri Sakti Sedyo Hartono Sigit Dwi Maryanto Siti Nur Azizah Fauziati Rahma, Siti Nur Azizah Fauziati Sri Wahyuningsih Sudarsono . SUHARYANTO SUHARYANTO Sujadmiko, Heri Sukarti Moeljopawiro Supriyadi Supriyadi Susamto Somowiyarjo Tri Joko Trijoko Trijoko Tuty Arisuryanti, Tuty Ummah, Isna Mustafiatul Wayan Tunas Artama Wenny Deisshinta Rahmadani, Wenny Deisshinta Widya Asmara Wijaya, I Made Saka Wisnu Nurcahyo Y. Andi Trisyono Yambise, Hani Christin Yasir Sidiq Yuanita Rachmawati, Yuanita