Iswari S. Dewi
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111

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INDUKSI KALUS DAN REGENERASI TIGA GENOTIPE TOMAT (SOLANUM LYCOPERSICON L.) MELALUI KULTUR ANTERA Ningsih, Ratna; Purwoko, Bambang S.; Syukur, Muhamad; Dewi, Iswari S.
Jurnal Hortikultura Indonesia Vol. 7 No. 2 (2016): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.486 KB) | DOI: 10.29244/jhi.7.2.75-82

Abstract

ABSTRACTThe aims of this research were to evaluate culture ability of 3 tomato genotypes through their androgenic response in callus induction and regeneration media. Completely randomized design with factorial arrangement and 5 replications were used. Treatments consisted of three genotypes (Tora, Ratna and hybrid variety Permata), six callus induction media in the first phase and three genotypes and two regeneration media. The result showed that hybrid variety Permata had the highest anther culture ability then others genotypes. Permata had the highest percentage of callus induction (27%) followed by Tora (14%) and Ratna (12%). The highest percentage of callus induction was shown in DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA media (39.7%) followed by DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA media (33.0%). Both genotypes and media gave low percentage of shoot induction. The percentage of shoot induction in hybrid variety Permata was 4.2% while in Tora was 2.1% and Ratna was 0%. The percentage of shoot induction in MS + 25 mg L-1 Zeatin was 2.8% while in MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA was 1.4%.Keywords: androgenesis, auxin, cytokinine, in vitro, medium, tomato ABSTRAKPenelitian ini bertujuan untuk mengetahui daya kultur antera tiga genotipe tomat melalui percobaan induksi pembentukan kalus dan regenerasi tunas. Percobaan dirancang menggunakan Rancangan Acak Lengkap faktorial dengan lima ulangan. Bahan tanam yang digunakan ialah tomat varietas Tora, Ratna dan varietas hibrida Permata. Media yang digunakan adalah 6 media induksi kalus dan 2 media regenerasi tunas. Hasil penelitian menunjukkan bahwa tomat varietas hibrida Permata memiliki daya kultur antera yang lebih baik dibandingkan genotipe lainnya. Permata memiliki persentase jumlah kalus 27% lebih tinggi dibandingkan Tora (14%) dan Ratna (12%). Media yang paling baik menginduksi kalus adalah media DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA (39%) dan DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA (33%). Baik genotipe maupun media yang digunakan menghasilkan jumlah tunas yang rendah. Persentase induksi tunas varietas hibrida Permata 4.2% lebih tinggi dibandingkan Tora (2.1%) dan Ratna (0%). Persentase induksi tunas media MS + 0.25 mg L-1 Zeatin (2.8%) lebih tinggi dibandingkan media MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA (1.4%).Kata kunci: androgenesis, auksin, in vitro, media, sitokinin, tomat
CHARACTERIZATION OF DOUBLED HAPLOID DERIVED FROM ANTHER CULTURE FOR NEW TYPE UPLAND RICE Herawati, Reny; Purwoko, Bambang Sapta; Dewi, Iswari S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 3 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.982 KB) | DOI: 10.24831/jai.v38i3.14244

Abstract

Anther culture is one of tissue culture methods which can be applied to plant breeding programs in order to accelerate the process of obtaining pure lines. The successful development of rice varieties is highly dependent on genetic diversity and desirable traits. To obtain the genetic variability of doubled haploid lines through anther culture techniques, anther F1 or F2 were used as explants sources. The objectives of the study were to select and characterize doubled haploid lines of upland rice having the characters of new plant type, and to study the genetic variability and agronomic characters of tested doubled haploid lines. A total of 58 doubled haploid lines, and four parental lines i.e. Fatmawati, SGJT-28, SGJT-36, and Way Rarem were used in this study. The experiment used completely randomized design with three replications. Results showed that the characters of the doubled haploid lines vary considerably. Selection of the character i.e. number of productive tillers, number of fi lled grain per panicle, and percentage of empty grain was more effective to be selected because they were well correlated to weight of grain per hill, possessed high heritability values, and have wide genetic variability. Based on productive tillers number, number of fi lled grains per panicle, fertility, weight of 1,000 grains, and weight of grains per hill, the lines of P3-26, P3-27, P3-28, P4-45, P5-50, P6-103, P6-105, P3-120, P3-134, P3-135, P3-150, P3-158, P3-248, P3-249, P6-271, P6-272, P6-274, P6-276, and P6-295 were potential for further selection for new type of upland rice.
ANTHER CULTURE ABILITY FROM CROSSESS BETWEEN UPLAND AND NEW PLANT TYPES OF RICE Sa?tri, Heni; Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari S.; Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.417 KB) | DOI: 10.24831/jai.v38i2.1790

Abstract

Anther culture provides rapid route in obtaining pure lines in a single generation through producing green haploid plants that may be spontaneously doubled. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of eight F1s derived from crossess between upland and new plant types of rice and from their four parents through anther culture. Completely randomized design with 25 replications was used in this research. Treatments consisted of four parent lines/varieties i.e. P1 (Fatmawati and BP360E-MR-79-2), P2 (Fulan Telo Gawa and Fulan Telo Mihat) and eight F1s obtained from reciprocal crosses of P1 and P2. Callus induction medium was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1  kinetin + 10-3  M Putrescine, while regeneration medium was based on MS + 0.5 mg L-1  NAA + 2.0 mg L-1  kinetin + 10-3  M Putrescine. The result indicated that F1 derived from Fatmawati x Fulan Telo Gawa (5.00% green plants per total anther) and their reciprocal (3.80% green plants per total anther) crosses were the most responsive genotypes in rice anther culture (had high anther culture ability). The F1 genotypes were more effective to produce green and doubled haploid plants in rice anther culture than their parents. From this research, 161 double haploid plants (29.81%) from total acclimated green plantlets were obtained.  Keywords: anther culture, upland rice, new plant type of rice
KERAGAMAN GENETIK DAN KARAKTER AGRONOMI GALUR HAPLOID GANDA PADI GOGO DENGAN SIFAT-SIFAT TIPE BARU HASIL KULTUR ANTERA Herawati, Reny; Purwoko, Bambang S.; Dewi, Iswari S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 2 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.979 KB) | DOI: 10.24831/jai.v37i2.1399

Abstract

The development  of new plant type of upland rice in relatively short time can be done by using anther culture technique. The technique has been recognized as a rapid and efficient technology for plant improvement. Plant materials  used in this research were 320 s doubled haploid lines derived from anther culture and their 4 parents   namely Fatmawati, SGJT-28, SGJT-36 and Way Rarem. Observation was conducted on  plant height, number of productive tillers, days of flowering and maturity, length of panicle, number of grains per panicle,  number of filled grains per panicle, percentage of unfilled grains, weight of 1000 grains and weight of grains per hill. The result showed that there were broad variations in the agronomic characters of doubled haploid lines. There were 58 upland rice lines can be obtained with new plant type characters (NPT).  Several lines showed superior characters. Line P6-105, P3-134, P3-135, P3-175 had productive tiller more than that of parental, P3-160, P3-196, P6-274  had long panicle, number of grain per panicle and low percentage of unfilled grain,  P3-135, P6-271, P6-274, and P6-276 had weight of grains per hill. These lines had potential as new plant type of upland rice. Length of panicle, number of grains per panicle,  number of filled grains per panicle, and weight of grains per hill had high heritability and wide genetic variability.   Key words: Genetic variability,  doubled haploid, anther culture, upland rice  
EVALUASI GALUR HAPLOID GANDA PELESTARI HASIL KULTUR ANTERA UNTUK PERAKITAN GALUR MANDUL JANTAN PADA PADI Rumanti, Indrastuti A.; Dewi, Iswari S.; Purwoko, Bambang S.; Aswidinnoor, Hajrial
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (64.008 KB) | DOI: 10.24831/jai.v37i1.1387

Abstract

Hybrid rice has been proven to be the key factor to increase food supply in highly populated countries such as China and India. The success has encouraged the government of Indonesia to intensify research and development on hybrid rice using cytoplasmic-genetic male sterility system. The use of good male sterile line is a prerequisite for commercial seed production of hybrid rice.  The objective of this research was to evaluate several F1 plants to develop completely sterile cytoplasmic male sterile line (CMS) with good agronomic characters.  This research was conducted at screen house of Bogor Agricultural University during dry season (DS) of 2007.  Fourty eight F1s, derived from testcross between cytoplasmic male sterile sources with doubled-haploid (DH) maintainer lines, were evaluated in randomized complete design using three replications.  Observation was done on pollen sterility, panicle and stigma exsertion, plant height, and tillering ability.  The results showed 14 F1s were completely sterile (100 % pollen sterility).  They were A-2/H36-3-Ma, A-1/H36-3-Mb, A-2/H36-3-Mb, A-3/H36-3-Mb, A-1/H36-4-M, A-2/B1-1-Mb, A-3/B1-1-Mb, A-1/B1-2-Pa, A-2/B1-2-Pb, A-3/B2-1-M, A-1/B2-4-Pb, A-3/B4-1-Da, A-1/B4-1-Dc, and A-3/B4-1-Dc.  They also had early flowering date, averaged from 66 - 90 days after planting (DAP).  There were other 14 F1s with stigma exsertion more than 70 % and good panicle exsertion, i.e.  A-2/H36-3-Mb, A-1/H36-3-Mc, A-2/H36-3-Mc, A-1/H36-4-M, A-2/B1-2-Pa, A-2/B1-2-Pb, A-3/B2-1-Db, A-1/B2-1-Dc, A-1/B2-1-M, A-1/B2-4-Pb, A-3/B2-4-Pb, A-2/B4-1-Da, A-1/B4-1-Dc and A-3/B4-1-Dc.  However, only five F1s had all desirable characteristics as male sterile line candidates, such as 100% pollen sterility, good panicle exsertion, high stigma exsertion, semi-dwarf in plant height and good tillering ability.  They were A-1/H36-4-M, A-2/B1-2-Pb, A-1/B2-4-Pb, A-1/B4-1-Dc, A-3/B4-1-Dc.   Key words:  Doubled-haploid maintainer lines, CMS, pollen sterility, hybrid rice
KARAKTER MORFOLOGI DAN KIMIA 18 KULTIVAR PAMELO (CITRUS MAXIMA (BURM.) MERR.) BERBIJI DAN TANPA BIJI Susanto, Slamet; Rahayu, Arifah; Sukma, Dewi; Dewi, Iswari S.
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Pamelo is one of the oranges species which have variety of form, size, colour and taste. Most of the pamelo cultivars with seeds, while part of it is seedless. The concumen prefer to chose seedles than with seed because they could consume more. The research proposed to know the morphologycal and chemical characteristics of pamelo with seed and seedless. Characteristication done in RGCI and port harvesting laboratory at IPB to the Pamelo come from Sumedang, Pati, Kudus, Magetan, Aceh and Pangkep (South Sulaewsi) in the period of April 2009 until July 2010. The result of research shown that several pamelo seedless cultivars have pyriform, while other with seed have spheroid form. A few fruit from pamelo seedless cultivars have sweet taste until less taste, with pH of its juice vary from 6.2-6.3, except Jawa cultivar which have pH 4 which its total tertiration acid is 0.47- 0.50 g/g, PTT 9.8- 11.0 ( 0brix), PTT/ATT 19,5-25,3. Vitamine C content 38-48.2 mg/100 g and narigin content from 118,3-1063,2 mg/ml, while pamelo with seed have taste sweet acid, with fruit juice pH 3.7 - 4.7, except "red bali 1", which have pH 6.0, ATT 0,35 - 0,59 g/g, PTT 8.7 - 11.3 ( 0brix), PTT/ATT 16.9 - 24.6, vita mine C content 28.6 - 43.8 and narigin content 55.2 - 461.2 mg/ml. 
ROLE OF POLYAMINES IN INHIBITION OF ETHYLENE BIOSYNTHESIS AND THEIR EFFECTS ON RICE ANTHER CULTURE DEVELOPMENT Dewi, Iswari S.; Purwoko, Bambang S.
Indonesian Journal of Agricultural Science Vol 9, No 2 (2008): October 2008
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

The polyamines such as putrescine, spermidine, and sperminewere reported to increase green plant regeneration in rice antherculture. Low response of anther culture of rice sub-speciesindica may be improved with the addition of putrescine in theculture media. Four experiments were conducted to study therole of polyamines in inhibition of ethylene biosynthesis andtheir effects on rice anther culture development. Anthers oftwo subspecies of rice, indica (IR64, Krowal, Jatiluhur) andjaponica (Taipei 309) were cultured onto media supplementedwith putrescine (N6P) and without putrescine (N6). Youngpanicles containing the anthers at mid-to-late nucleatemicrospores were cold pretreated at 5 + 2°C and incubated inthe dark for 8 days before the anthers were cultured. Resultsshowed that medium without putrescine produced an earliersenescence of indica rice anther than that of japonica. Theaddition of 10-3 M putrescine into the culture media inhibitedethylene biosynthesis as anther senescence delayed, increasedthe three polyamines contents, and decreased the ACC contentas well as ACC oxydase activity in anther-derived calli. In theanther and anther-derived calli of subspecies indica, the totalpolyamines content was lower (10.14 nM g-1 anther and 8.48 nMg-1 calli) than that of subspecies japonica (12.61 nM g-1 antherand 10.16 nM g-1 calli), whereas the ethylene production washigher (32.31 nM g-1 anther and 2.48 nM g-1 calli) than thejaponica (31.68 nM g-1 anther and 1.76 nM g-1 calli). This studysuggests that application of 10-3 M putrescine in anther cultureof rice subspecies indica improves androgenesis by inhibitingearly senescence of cultured anthers and enhancing embryo orcallus formation from microspores.
PEMBENTUKAN GALUR HAPLOID GANDA PADI GOGO DENGAN SIFAT-SIFAT TIPE BARU MELALUI KULTUR ANTERA Herawati, Reny; Purwoko, Bambang S.; Khumaida, Nurul; Dewi, Iswari S.; Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.735 KB) | DOI: 10.24831/jai.v36i3.1375

Abstract

The breeding of upland rice with New Plant Type characters in relatively short time can be done by using anther culture technique. The technique has been recognized as a rapid and efficient technology for crop improvement. Plant materials used in this research were F1 crossing P1 (Fatmawati x Way Rarem), P2 (Fatmawati x SGJT-28),  P3 (Fatmawati x SGJT-36), P4  (Way Rarem x Fatmawati), P5 (SGJT-28 x Fatmawati), and P6  (SGJT-36 x Fatmawati). Media for calli induction (N6) and regeneration (MS) were according to Dewi methods (2003). The results of this study indicated that P3 (Fatmawati x SGJT-36) and P6 (SGJT-36 x Fatmawati)  from resiprocal crosses gave better response in anther culture than the others crosses for their calli induction and green plant regeneration. From the six F1 crosses, 348 (53.5%) spontaneous doubled haploid (DH) pure lines were obtained, six lines from Farmawati x Way Rarem, 13 lines from Fatmawati x SGJT-28, 187 lines from Fatmawati x SGJT-36, three lines from Way Rarem x Fatmawati, five lines from SGJT-28 x Fatmawati, and 134 lines from SGJT-36 x Fatmawati.  These lines should be evaluated and characterized for their agronomical and morphological  traits.    Key words: Anther culture, doubled haploid, callus induction, green plant regeneration        
THERAPY CYCLING TO ELIMINATE HIGH-TITERED, MULTIPLE VIRUS INFECTION IN VITRO POT A TO PLANTLETS Dewi, Iswari S.; Slack, Steven A.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 22 No. 2 (1994): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1260.867 KB) | DOI: 10.24831/jai.v22i2.1640

Abstract

A protocol for treatment of in vitro potato plantlets to eliminate systemic viruses was established. Efficiency decreased, however, for selected genotypes when a virus was high-titered and/or multiple viruses existed Modified nodal cutting (Mncs =  
ANALISIS KORELASI DAN KOEFISIEN LINTAS ANTAR BEBERAPA SIFAT PADI GOGO PADA MEDIA TANAH MASAM Bakhtiar, Bakhtiar; Purwoko, Bambang S.; Trikoesoemaningtyas, Trikoesoemaningtyas; Dewi, Iswari S.
Jurnal Floratek Vol 5, No 2 (2010)
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

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Abstract

A study was conducted to study correlation between some characters of upland rice and path coefficient analysis, which will be helpful for further selection process to improve upland rice in acid soils. The results of evaluation of lines for Al-tolerant in acid soils revealed that number of tillers and filled grain per panicle were inherited with high heritability and directly affected grain weight and contributed indirectly to the correlation between grain weight and other characters. However, indirect selection through number of tillers and filled grain per panicle was less efficient than direct selection through grain weight.