Iswari Saraswati Dewi
Balai Besar Bioteknologi dan Sumberdaya Genetika Pertanian Jl. Tentara Pelajar No. 3A Bogor 16111, Indonesia

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KULTUR ANTERA UNTUK MENDAPATKAN GALUR PADI TOLERAN SALINITAS Safitri, Heni; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Ardie, dan Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.975 KB) | DOI: 10.24831/jai.v44i3.12770

Abstract

ABSTRACTHaploid breeding through anther culture allows shortening of the breeding cycle and production of homozygous lines from a segregating population in the immediate generation. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of twelve F1s, derived from reciprocal crossing between high yielding rice variety and rice tolerance to salinity, through anther culture. Completely randomized design with 20 replications was used in this research. Medium for callus induction was based on N6 medium + 2.0 mg NAA L-1 + 0.5 mg kinetin L-1 + 1 mM putrescine, while regeneration medium was based on MS + 0.5 mg NAA L-1 + 2.0 mg kinetin L-1 + 1 mM putrescine. Rooting were done in MS medium + 0.5 mg IBA L-1 + 1 mM putrescine. The result indicated that F1 derived from IR77674/Inpari 29 (3.1% green plants/total anther) was the most responsive genotypes in rice anther culture (high anther culture ability). After greenhouse grow out 125 putative double haploid plants were obtained (41.5% from total acclimated green plantlets).  Keywords: double haploid, green planlets, indica rice, salt tolerance
PENAMPILAN GALUR HARAPAN MUTAN DIHAPLOID PADI TIPE BARU DI SULAWESI SELATAN Dewi, Iswari Saraswati; Lestari, Endang Gati; ,, Chaerani; Yunita, Rossa
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.161 KB) | DOI: 10.24831/jai.v43i2.10408

Abstract

ABSTRACTSouth Sulawesi is known as one of national rice production centers. However, average productivity of rice varieties planted in that area (4.43 ton ha-1) is lower than those of rice productivity in Java (5.25 ton ha-1). The aims of this research were to evaluate agronomic characters and adaptation of 7 dihaploid mutant advanced lines of new plant type (DH-NPT) of rice at several locations in South Sulawesi. The research was conducted in 2012 at Maros, Gowa, Barru, and Pangkep. The experiments were conducted in randomized complete block design with 3 replications nested in locations. Treatment consisted of 7 DH-NPT of rice, i.e., BIO-MF115, BIO-MF116, BIO-MF125, BIO-MF130, BIO-MF133, BIO-MF151, BIO-MF153, and control varieties i.e., Fatmawati, Ciherang, and Inpari13. The results indicated that in general the lines had medium height (102.77-110.23 cm), moderate productive tiller (9-16 tiller per hill), moderate days to flower (50%), i.e., 73-76 days after sowing (DAS), earlier days to harvest (103-110 DAS), moderate panicle length (28.35-29.31 cm), large number of grain per panicle (> 250 grains) with moderate panicle fertility (63-70%), moderate 1,000 grain weight, i.e., 26.51-27.75 g, and high yield (7.51-8.09 ton ha-1). Four lines, i.e., BIO-MF116, BIO-MF130, BIO-MF151, and BIO-MF153 were stable and had wide adaptability. Other lines, i.e., BIO-MF125 and BIO-MF133 were sensitive to environmental changes, therefore they were classified as specifically adapted to favorable environment; while BIO-MF115 was not sensitive to environmental changes, and therefore it was adapted to non-favorable environment.Keywords: adaptation, agronomic characters, rice mutant
PENAMPILAN AGRONOMI DAN UJI TOLERANSI NAUNGAN GALUR DIHAPLOID PADI GOGO HASIL KULTUR ANTERA Mara, Kartika Kirana Sangga; Purwoko, Bambang Sapta; ,, Eko Sulistyono; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.581 KB) | DOI: 10.24831/jai.v43i1.9580

Abstract

ABSTRACTShifting of upland rice cultivation to marginal land faces drought stress, while planting under young plantation faces shading stress. There should be lines or varieties with good agronomic character and tolerant to the shading stress. Lines used in this experiment were obtained  from anther culture, i.e., III3-4-6-1, I5-10-1-1, WI-44, GI-7, O18-b-1, IW-67, IG-19, IG-38, IW-56, B13-2-e. Two experiments were conducted, i.e., agronomic performance experiment and shading tolerance experiment with a quick test method at seedling phase. Batutegi and Way Rarem were used as check varieties in the agronomic experiment. In shading tolerance experiment, Kalimutu was used as sensitive check and Jatiluhur was tolerant check varieties. The results of experiment indicated that B13-2-e (4.64 ton ha-1) and WI-44 line (4.05 ton ha-1) dihaploid lines showed high grain yield and comparable to Way Rarem (4.95 ton ha-1). B13-2-e and WI-44 lines were tolerant to shading. Another tolerant lines to shading, i.e., I5-10-1-1, O18-b-1, and IW-56. B13-2-e and WI-44 lines were recommended to advanced verification on multiple cropping system.Keywords: dry land, multiple cropping
REGENERASI DAN AKLIMATISASI KULTUR ANTERA ENAM PERSILANGAN F1 PADI SAWAH Gunarsih, Cucu; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Syukur, dan Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.596 KB) | DOI: 10.24831/jai.v44i2.13479

Abstract

ABSTRACTThe breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing:  INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM  putresin, and media for rooting was based on  MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and  Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%,  respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants),  G5 (21 plants), and G6 (26 plants).Keywords: Callus induction, doubled haploid, rice
POTENSI DAN STABILITAS HASIL, SERTA ADAPTABILITAS GALUR-GALUR PADI GOGO TIPE BARU HASIL KULTUR ANTERA ., Purbokurniawan; Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1517.935 KB) | DOI: 10.24831/jai.v42i1.8142

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ABSTRACTThe objectives of the research were to obtain information on yield potential, adaptability and stability of the upland rice lines. Ten lines and two cultivars were planted at five different locations in November 2010-March 2011. In each location, the experimental design was randomized complete block design with four replications. Observation was done on grains weight per hectar. The results showed that FM1R-1-3-1 achieved the highest productivity (5.65 ton ha-1). Genotype FG1R-36-1-1 was classified as stable genotype by four yield stability analysis as followed: Francis-Kannenberg, Finlay-Wilkinson, Eberhart-Russell and AMMI. Genotypes FG1-70-2-1, FG1R-30-1-5, FG1R-30-1-4 and FG1R-30-1-3 were classified as stable genotypes by three yield stability analysis: Finlay-Wilkinson, Eberhart-Russell and AMMI. AMMI  analysis showed that FG1-6-1-2, FG1-65-1-2, FG1R-30-1-1, FM1R-1-3-1, Fat-4-1-1, Situ Bagendit and Towuti as specific genotypes in certain environment.Keywords: dynamic, new plant type, specific, static, yield
PENENTUAN INDEKS SELEKSI UNTUK GALUR DIHAPLOID PADI SAWAH TADAH HUJAN BERDAYA HASIL TINGGI Akbar, Miftahur Rizqi; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Sugiyanta, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 2 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.905 KB) | DOI: 10.24831/jai.v47i2.25032

Abstract

Rainfed rice breeding for high yield is an alternative to increase national rice production. The breeding can beaccelerated using anther culture technique. The selection of high productivity lines may be more effective if it also involvesthe traits contributing to or affecting the yield traits. This study aimed at determining suitable characters as selection criteriaand obtain a selection index model for high yielding doubled haploid rainfed rice lines. The experiment was conductedin a greenhouse of ICABOGRAD, Bogor and Sawah Baru Experimental Station, Bogor Agricultural University using arandomized complete block design (RCBD) with three replications. Thirty doubled haploid lines derived from anther cultureand two check varieties namely Ciherang and Inpari 18 were used. The results showed that plant height (TT), number of filledgrain per panicle (GI), and productivity (PRD) could be used as suitable selection characters. The determination of selectionindex obtained a selection model which was Selection index = 0.48 PRD + 0.31 JGI - 0.31 TT. This model can be used toselect high yielding doubled haploid rainfed rice lines.
PEMILIHAN KARAKTER AGRONOMI UNTUK SELEKSI PADA GALUR-GALUR PADI DIHAPLOID HASIL KULTUR ANTERA Akhmadi, Gerland; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Wirnas, dan Desta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (732.425 KB) | DOI: 10.24831/jai.v45i1.13681

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Anther culture technique is able to accelerate plant breeding activities. The objectives of this research was to determine the agronomic traits that could be used for selection of the dihaploid rice line population through the calculation of heritability, genotypic correlation, path analysis and selecting dihaploid rice lines. The plant material used was 65 dihaploid rice lines DH 1 of F1 anther culture plants and Ciherang and Inpari 13 as check varieties. Agronomic characters that could be used as selection character are generative plant height, number of filled grain per panicle, the total number of grains per panicle, weight of 1,000 grains, and grain per hill. Twenty three dihaploid lines were selected based good agronomic characters with criteria generative plant height between 80-120 cm, number of filled grains per panicle > 100, number of grains per panicle > 120, weight of 1,000 grains > 20 g, and grain per hill > 25 g.Keywords: anther culture, heritability, agronomic characters
SKRINING AWAL TOLERANSI GALUR-GALUR DIHAPLOID PADI GOGO TERHADAP CEKAMAN KEKERINGAN PADA STADIA BIBIT Kartina, Nita; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Wirnas, Desta; Nindita, dan Anggi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.62 KB) | DOI: 10.24831/jai.v47i1.22766

Abstract

Development of doubled haploid lines of upland rice through anther culture is one way to obtain superior upland rice varieties tolerant to drought. The objectives of this research were to determine the response of doubled haploid lines to drought stress at seedling stage and to select tolerant doubled haploid lines. The research was conducted at a screenhouse of Muara Research Station, Indonesia Center of Rice Research (ICRR) from November to December 2017. Fourteen doubled haploid lines and 4 check varieties, namely Inpago 10 and Limboto, Salumpikit (drought tolerant check) and IR 20 (drought sensitive check) were used in this study. The experiment was arranged in a randomized complete block design with three replications. The observations were conducted on leaf rolling, leaf drought, and recovery ability. Based on Friedman nonparametric analysis, HR-5-7-1-1 and HR-7-15-2-1 had moderate response with estimated median values for leaf rolling of 3.44 and 3.00, respectively. Three doubled haploid lines namely HR-2-27-2-7, HR-2-34-1-3 and HR-7-15-2-1 had moderate response to drought with estimated median value of leaf drought of 3.56. Nine lines of doubled haploid had tolerant response with estimated median value of recovery ability of 1.00-1.56. Based on weighted selection index, nine doubled haploid lines with positive and high selection index were identified; however, only two lines (HR-7-15-2-1 and B3-2) had selection index higher than Inpago 10 and Limboto.Keywords: drought tolerance, leaf rolling, recovery, weighted selection index
PENENTUAN INDEKS SELEKSI TOLERANSI KEKERINGAN GALUR DIHAPLOID PADI SAWAH TADAH HUJAN PADA FASE PERKECAMBAHAN Akbar, Miftahur Rizqi; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, dan Willy Bayuardi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.866 KB) | DOI: 10.24831/jai.v46i2.19086

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Rainfed rice tolerant to drought and high yielding would be an alternative to rainfed lowland areas prone to drought stress. Selection in the early phase of plant growth will accelerate the effort to obtain rainfed varieties. The objective of this experiment was to identify characters that significantly affect the selection of drought tolerance using PEG 6000 concentration of 25% in the germination phase. The experiment was conducted in greenhouse of ICABIOGRAD, Bogor, using a factorial randomized complete block design with three replications. The first factor was 32 rice genotypes while the second factor was 2 levels of PEG 6000, i.e., concentrations of 0 and 25%. The results showed that the correlation analysis and principal component analysis obtain the important characters namely the germination percentage, seminal root length, and the dry weight of seminal roots. Based on discriminant analysis, the index matching value of 96.77% was suitable to distinguish tolerant and sensitive genotypes using PEG 6000 concentration of 25% in the germination phase.
HERITABILITAS, KARAKTERISASI, DAN ANALISIS CLUSTERGRAM GALUR-GALUR PADI DIHAPLOID HASIL KULTUR ANTERA Anshori, Muhammad Fuad; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Ardie, Sintho Wahyuning; Suwarno, Willy Bayuardi; Safitri, dan Heni
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.968 KB) | DOI: 10.24831/jai.v46i2.18377

Abstract

Doubled haploid rice formation through anther culture becomes one of the alternatives in the plant breeding. The lines need to be characterized, clustered, and selected. This study aimed at determining heritability, characters, and cluster of doubled haploid lines obtained from anther culture. This experiment was conducted at IPB Rice Field Experiment Station, Darmaga, Bogor from October 2016 until January 2017. The experiment was managed according to randomized complete block design (RCBD) with 3 replications and genotype as the single factor treatment. Genotypes used were 60 genotypes consisting of 56 doubled haploid lines and 4 control varieties. The controls were Ciherang, Inpara 5, Inpari 29, and Inpari 34 Salin Agritan. The results showed that all characters have significant effect and high heritability (above 60%), so they could be used as a selection character. The tested genotypes could be grouped into three main groups. The first group consisted of 11 genotypes, the second group consisted of 44 genotypes, and the third group consisted of 5 genotypes. The main group determinant characters were productive tillers, percentage of filled grain number, plant height, panicle length, and weight of 1,000 grains. The number of filled grain and productive tillers can be used as selection characters together with yield potential.